LOCUS       NM_010477               2347 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus heat shock protein 1 (chaperonin) (Hspd1), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_010477
VERSION     NM_010477.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Xu,L., Tan,C., Barr,J., Talaba,N., Verheyden,J., Chin,J.S.,
            Gaboyan,S., Kasaraneni,N., Elgamal,R.M., Gaulton,K.J., Lin,G.,
            Afshar,K., Golts,E., Meier,A., Crotty Alexander,L.E., Borok,Z.,
            Shen,Y., Chung,W.K., McCulley,D.J. and Sun,X.
  TITLE     Context-dependent roles of mitochondrial LONP1 in orchestrating the
            balance between airway progenitor versus progeny cells
  JOURNAL   Cell Stem Cell 31 (10), 1465-1483 (2024)
   PUBMED   39181129
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Shen,T., Wang,S., Huang,C., Zhu,S., Zhu,X., Li,N., Wang,H.,
            Huang,L., Ren,M., Han,Z., Ge,J., Chen,Z. and Ouyang,K.
  TITLE     Cardiac-specific deletion of heat shock protein 60 induces
            mitochondrial stress and disrupts heart development in mice
  JOURNAL   Biochem Biophys Res Commun 710, 149883 (2024)
   PUBMED   38588611
  REMARK    GeneRIF: Cardiac-specific deletion of heat shock protein 60 induces
            mitochondrial stress and disrupts heart development in mice.
REFERENCE   3  (bases 1 to 2347)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 2347)
  AUTHORS   Schraps,N., Tirre,M., Pyschny,S., Reis,A., Schlierbach,H.,
            Seidl,M., Kehl,H.G., Schanzer,A., Heger,J., Jux,C. and
            Drenckhahn,J.D.
  TITLE     Cardiomyocyte maturation alters molecular stress response
            capacities and determines cell survival upon mitochondrial
            dysfunction
  JOURNAL   Free Radic Biol Med 213, 248-265 (2024)
   PUBMED   38266827
REFERENCE   5  (bases 1 to 2347)
  AUTHORS   Radzishevsky,I., Odeh,M., Bodner,O., Zubedat,S., Shaulov,L.,
            Litvak,M., Esaki,K., Yoshikawa,T., Agranovich,B., Li,W.H.,
            Radzishevsky,A., Gottlieb,E., Avital,A. and Wolosker,H.
  TITLE     Impairment of serine transport across the blood-brain barrier by
            deletion of Slc38a5 causes developmental delay and motor
            dysfunction
  JOURNAL   Proc Natl Acad Sci U S A 120 (42), e2302780120 (2023)
   PUBMED   37812701
REFERENCE   6  (bases 1 to 2347)
  AUTHORS   Brudzynski,K., Martinez,V. and Gupta,R.S.
  TITLE     Immunocytochemical localization of heat-shock protein 60-related
            protein in beta-cell secretory granules and its altered
            distribution in non-obese diabetic mice
  JOURNAL   Diabetologia 35 (4), 316-324 (1992)
   PUBMED   1516759
REFERENCE   7  (bases 1 to 2347)
  AUTHORS   Ikawa,S. and Weinberg,R.A.
  TITLE     An interaction between p21ras and heat shock protein hsp60, a
            chaperonin
  JOURNAL   Proc Natl Acad Sci U S A 89 (6), 2012-2016 (1992)
   PUBMED   1347942
REFERENCE   8  (bases 1 to 2347)
  AUTHORS   Elias,D., Reshef,T., Birk,O.S., van der Zee,R., Walker,M.D. and
            Cohen,I.R.
  TITLE     Vaccination against autoimmune mouse diabetes with a T-cell epitope
            of the human 65-kDa heat shock protein
  JOURNAL   Proc Natl Acad Sci U S A 88 (8), 3088-3091 (1991)
   PUBMED   1707531
REFERENCE   9  (bases 1 to 2347)
  AUTHORS   Lotscher,E. and Allison,J.P.
  TITLE     Nucleotide and deduced amino acid sequence of a murine cDNA clone
            encoding one member of the hsp65 multigene family
  JOURNAL   Nucleic Acids Res 18 (23), 7153 (1990)
   PUBMED   2263486
REFERENCE   10 (bases 1 to 2347)
  AUTHORS   Venner,T.J. and Gupta,R.S.
  TITLE     Nucleotide sequence of mouse HSP60 (chaperonin, GroEL homolog) cDNA
  JOURNAL   Biochim Biophys Acta 1087 (3), 336-338 (1990)
   PUBMED   1979012
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY014148.1, AK143882.1 and BF607107.1.
            
            On Apr 17, 2008 this sequence version replaced NM_010477.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146831.1, SRR1660819.118464.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                BY014148.1         2-97
            97-2329             AK143882.1         2-2234
            2330-2347           BF607107.1         760-777
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 28.01 cM"
     gene            1..2347
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /note="heat shock protein 1 (chaperonin)"
                     /db_xref="GeneID:15510"
                     /db_xref="MGI:MGI:96242"
     exon            1..154
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            155..330
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     CDS             157..1878
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /EC_number="5.6.1.7"
                     /note="heat shock 60kDa protein 1 (chaperonin); chaperonin
                     60; 60 kDa chaperonin; heat shock protein 60;
                     mitochondrial matrix protein P1; heat shock protein, 60
                     kDa"
                     /codon_start=1
                     /product="60 kDa heat shock protein, mitochondrial"
                     /protein_id="NP_034607.3"
                     /db_xref="CCDS:CCDS35569.1"
                     /db_xref="GeneID:15510"
                     /db_xref="MGI:MGI:96242"
                     /translation="MLRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGV
                     DLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVA
                     NNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSK
                     PVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMK
                     FDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAE
                     DVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNL
                     EDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNE
                     RLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPA
                     LDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLG
                     DFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGG
                     GMGGGMF"
     exon            331..583
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            584..666
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            667..762
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            763..856
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            857..1025
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1125
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1371
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            1372..1546
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1725
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..2332
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2311..2316
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /note="hexamer: AATAAA"
     polyA_site      2332
                     /gene="Hspd1"
                     /gene_synonym="60kDa; CPN60; HSP-60; HSP-65; Hsp60"
                     /note="major polyA site"
ORIGIN      
        1 gagagcgggg ggcgcgcgcg cgcacacgcg gggccgcggg gcggggcggg gagggagggg
       61 actcgggctc attgcggtgc gcgcccagag ctgggtccct cactcgccgc agacggcctg
      121 cctcgcctcg tgctctcctg ccgccgcccc gcagaaatgc ttcgactacc cacagtcctt
      181 cgccagatga gaccagtgtc ccgggcactg gctcctcatc tcactcgggc ctatgccaaa
      241 gatgtaaaat ttggtgcgga cgctcgagcc ttaatgcttc aaggtgtaga ccttttagca
      301 gatgctgtag ctgttacaat ggggccaaag ggaagaactg tgattattga acagagttgg
      361 ggaagtccca aagtaacaaa agatggggtc actgttgcaa agtcaattga tttaaaggat
      421 aaatacaaaa atattggagc taaacttgtt caggacgttg ccaataacac aaacgaagag
      481 gctggggatg gcaccaccac tgccactgtt ctggcacgat ctattgccaa ggagggcttt
      541 gagaagatca gcaaaggggc taatccagtg gaaatccgga gaggtgtgat gttggctgtg
      601 gatgctgtaa ttgctgaact taagaaacag tctaaacctg tgacaacccc tgaagaaatt
      661 gctcaggttg ctacaatttc tgcaaatgga gacaaagaca ttgggaacat catttctgat
      721 gcaatgaaaa aggttggaag aaagggtgtc atcacagtga aggatggaaa aaccctgaat
      781 gatgagctag aaattattga aggcatgaag tttgatagag gatatatttc cccgtatttt
      841 attaacacat caaaaggtca aaagtgtgaa ttccaagatg cctatgtctt gttgagtgaa
      901 aagaaaattt ccagtgttca gtccattgtc cctgctcttg aaattgctaa tgctcatcgg
      961 aagccattgg tcataatcgc cgaagacgtt gacggagaag ctctaagcac gctggttttg
     1021 aacaggctaa aagttggtct tcaggttgtg gcagtcaaag ctccaggatt tggggacaat
     1081 aggaagaacc agcttaaaga tatggctatt gctactggtg gtgcagtgtt tggagaagag
     1141 gggttgaatc taaatcttga agatgttcaa gctcatgact taggaaaagt tggggaggtc
     1201 attgtcacca aagatgatgc catgcttttg aaaggaaaag gtgacaaagc tcacattgaa
     1261 aaacgtattc aagaaatcac tgagcagcta gacatcacaa ctagtgaata tgaaaaagaa
     1321 aagctgaacg agcgacttgc taaactttca gatggagtag ctgtgttgaa ggttggagga
     1381 acaagtgatg ttgaagtgaa tgagaaaaaa gacagagtta ctgatgctct caatgctaca
     1441 agagcagctg ttgaagaagg cattgttcta ggagggggct gcgctctgct tcggtgcatc
     1501 ccagccttgg attcattaaa gcctgctaat gaagaccaga aaataggtat agaaattatt
     1561 aaaagagcac ttaaaattcc tgcaatgacg attgctaaga atgcaggtgt tgaaggatct
     1621 ttgatagttg agaaaattct gcagagttcc tcagaagttg gttatgacgc catgcttgga
     1681 gattttgtga acatggtgga aaaagggatc attgatccaa caaaggttgt gagaactgcc
     1741 ttactggatg ctgctggggt ggcctccttg ctaactacag ccgaagctgt agtgacagaa
     1801 attcctaaag aagagaagga ccctggaatg ggtgcaatgg gtggcatggg agggggtatg
     1861 ggaggcggca tgttctaact cctagagtag tgctttgccc ttatcaatga actgtgacag
     1921 gaagctcaag gcaggttcct caccaataac ttcagagaag tcacctggag aaaatgactg
     1981 aagagaaggc tggctgacca ctgtaatcat cagttactgg tttcctttga cgatatataa
     2041 tggtttactg ctgtcattgt ccatgcctac agataattta ttttgtattt ttgaataaag
     2101 acatttgtac attcctgatg ctgggtgcaa gagccatata ccagtgtcct gctttcaact
     2161 taaatcactg aggcatctct actgttctgt gagcatcagg actgtagcgc tgtgtcacca
     2221 catgagagtt cagaagcagc ctttctgtgg agggtgggaa tgattgtgta caaagtagag
     2281 aagtatccaa ttatgtgaca acctttgtgt aataaaattt tgtttaaagt ttaaaaaaaa
     2341 aaaaaaa
//
