LOCUS       NM_010481               3067 bp    mRNA    linear   ROD 28-JAN-2025
DEFINITION  Mus musculus heat shock protein 9 (Hspa9), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_010481
VERSION     NM_010481.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Zhao,F., Cui,Z., Wang,P., Zhao,Z., Zhu,K., Bai,Y., Jin,X., Wang,L.
            and Lu,L.
  TITLE     GRP75-dependent mitochondria-ER contacts ensure cell survival
            during early mouse thymocyte development
  JOURNAL   Dev Cell 59 (19), 2643-2658 (2024)
   PUBMED   38981469
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Zhang,J.R., Shen,S.Y., Zhai,M.Y., Shen,Z.Q., Li,W., Liang,L.F.,
            Yin,S.Y., Han,Q.Q., Li,B., Zhang,Y.Q. and Yu,J.
  TITLE     Augmented microglial endoplasmic reticulum-mitochondria contacts
            mediate depression-like behavior in mice induced by chronic social
            defeat stress
  JOURNAL   Nat Commun 15 (1), 5199 (2024)
   PUBMED   38890305
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3067)
  AUTHORS   Jang,S., Jang,S., Ko,J., Bae,J.E., Hyung,H., Park,J.Y., Lim,S.G.,
            Park,S., Park,S., Yi,J., Kim,S., Kim,M.O., Cho,D.H. and Ryoo,Z.Y.
  TITLE     HSPA9 reduction exacerbates symptoms and cell death in DSS-Induced
            inflammatory colitis
  JOURNAL   Sci Rep 14 (1), 5908 (2024)
   PUBMED   38467701
  REMARK    GeneRIF: HSPA9 reduction exacerbates symptoms and cell death in
            DSS-Induced inflammatory colitis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3067)
  AUTHORS   Bidooki,S.H., Sanchez-Marco,J., Martinez-Beamonte,R.,
            Herrero-Continente,T., Navarro,M.A., Rodriguez-Yoldi,M.J. and
            Osada,J.
  TITLE     Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6 and HSPA9
            to Regulate Glutathione Metabolism and Lipid Peroxidation in the
            Hepatic AML12 Cell Line
  JOURNAL   Int J Mol Sci 24 (24), 17131 (2023)
   PUBMED   38138960
  REMARK    GeneRIF: Endoplasmic Reticulum Protein TXNDC5 Interacts with PRDX6
            and HSPA9 to Regulate Glutathione Metabolism and Lipid Peroxidation
            in the Hepatic AML12 Cell Line.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3067)
  AUTHORS   Tscherner,A.K., McClatchie,T., Macaulay,A.D. and Baltz,J.M.
  TITLE     Relationship of quantitative reverse transcription polymerase chain
            reaction (RT-PCR) to RNA Sequencing (RNAseq) transcriptome
            identifies mouse preimplantation embryo reference genesdagger
  JOURNAL   Biol Reprod 109 (5), 601-617 (2023)
   PUBMED   37669129
REFERENCE   6  (bases 1 to 3067)
  AUTHORS   Dvorak,P., Dvorakova,D., Yoshiki,A., Ohashi,T., Kitamura,K. and
            Kusakabe,M.
  TITLE     Expression of paternal and maternal mitochondrial HSP70 family,
            hsc74, in preimplantation mouse embryos
  JOURNAL   Int J Dev Biol 39 (3), 511-517 (1995)
   PUBMED   7577442
REFERENCE   7  (bases 1 to 3067)
  AUTHORS   Merrick,B.A., Patterson,R.M., Witcher,L.L., He,C. and Selkirk,J.K.
  TITLE     Separation and sequencing of familiar and novel murine proteins
            using preparative two-dimensional gel electrophoresis
  JOURNAL   Electrophoresis 15 (5), 735-745 (1994)
   PUBMED   7523108
REFERENCE   8  (bases 1 to 3067)
  AUTHORS   Michikawa,Y., Baba,T., Arai,Y., Sakakura,T., Tanaka,M. and
            Kusakabe,M.
  TITLE     Antigenic protein specific for C3H strain mouse is a mitochondrial
            stress-70 protein
  JOURNAL   Biochem Biophys Res Commun 196 (1), 223-232 (1993)
   PUBMED   7692847
REFERENCE   9  (bases 1 to 3067)
  AUTHORS   Domanico,S.Z., DeNagel,D.C., Dahlseid,J.N., Green,J.M. and
            Pierce,S.K.
  TITLE     Cloning of the gene encoding peptide-binding protein 74 shows that
            it is a new member of the heat shock protein 70 family
  JOURNAL   Mol Cell Biol 13 (6), 3598-3610 (1993)
   PUBMED   7684501
REFERENCE   10 (bases 1 to 3067)
  AUTHORS   Kusakabe,M., Yokoyama,M., Sakakura,T., Nomura,T., Hosick,H.L. and
            Nishizuka,Y.
  TITLE     A novel methodology for analysis of cell distribution in chimeric
            mouse organs using a strain specific antibody
  JOURNAL   J Cell Biol 107 (1), 257-265 (1988)
   PUBMED   3292542
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC057343.1 and BP769296.1.
            
            On Dec 14, 2007 this sequence version replaced NM_010481.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052727.1, AK145965.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2824              BC057343.1         1-2824
            2825-3067           BP769296.1         1-243               c
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 18.8 cM"
     gene            1..3067
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /note="heat shock protein 9"
                     /db_xref="GeneID:15526"
                     /db_xref="MGI:MGI:96245"
     exon            1..182
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..2141
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /note="heat shock protein 9A; heat shock protein, A;
                     GRP-75; p66 MOT; peptide-binding protein 74; heat shock 70
                     kDa protein 9; 75 kDa glucose-regulated protein;
                     C3H-specific antigen; heat shock protein, 74 kDa, A; heat
                     shock protein cognate 74"
                     /codon_start=1
                     /product="stress-70 protein, mitochondrial"
                     /protein_id="NP_034611.2"
                     /db_xref="CCDS:CCDS29138.1"
                     /db_xref="GeneID:15526"
                     /db_xref="MGI:MGI:96245"
                     /translation="MISASRAAAARLVGTAASRSPAAARPQDGWNGLSHEAFRFVSRR
                     DYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVG
                     MPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDTKNVPFKIVRASNGDAWVEAHGKL
                     YSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLR
                     VINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGE
                     DFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTM
                     DASGPKHLNMKLTRAQFEGIVTDLIKRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRM
                     PKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETL
                     GGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGI
                     PPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAE
                     KYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRALLAG
                     KDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ"
     exon            183..241
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            242..329
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            330..511
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            512..636
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            637..710
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            711..817
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            818..980
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1073
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1283
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1511
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1616
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1734
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1735..1829
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1830..1922
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2063
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..3043
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2801..2806
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /note="hexamer: AATAAA"
     polyA_site      2824
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /note="major polyA site"
     regulatory      3020..3025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
                     /note="hexamer: AATAAA"
     polyA_site      3043
                     /gene="Hspa9"
                     /gene_synonym="74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a;
                     Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74"
ORIGIN      
        1 cgtggttgga ggtttccaga agcgtagcca ccaccgtgca cgcagctccg ggcccgtggg
       61 gtgttggttc ttgccctcgt aaccccctct gtccagccac catgataagc gccagcagag
      121 ccgcggccgc gcgtctcgtg ggcaccgctg cgtcccggag ccccgcagcc gcccgtcccc
      181 aggatggctg gaatggcctt agccatgagg cttttagatt tgtttcaaga agagattatg
      241 catcagaagc aatcaagggt gcagtggttg gtattgattt gggtactact aactcctgtg
      301 tggctgttat ggagggcaaa caagcaaagg tcctggagaa tgctgaaggt gccagaacta
      361 ccccttctgt ggttgccttt acagcagatg gagaacgact tgttggtatg ccagcaaaac
      421 ggcaagctgt caccaatcca aacaatacct tctatgctac taagcgtctt attggacgac
      481 gatatgatga ccctgaagta cagaaagaca ctaagaatgt tccttttaaa attgtccgtg
      541 cctccaatgg tgatgcttgg gttgaggctc atggaaaact ctattctcca agtcagattg
      601 gagcatttgt gttgatgaag atgaaagaga ctgcagaaaa ttacttgggc cacacagcaa
      661 aaaatgctgt gatcacagtc cctgcttatt tcaatgattc acagcgacag gccactaagg
      721 atgctggcca gatatctggg ctaaatgtgc ttcgagtgat caatgagcct acagctgctg
      781 ctctagctta cggtctggac aaatctgaag ataaagtcat tgctgtgtat gatttaggtg
      841 gtggaacctt tgacatttct atcctggaaa ttcagaaagg agtgtttgag gtgaaatcta
      901 ccaatgggga cactttctta ggaggggaag actttgacca agctttgttg cggcacattg
      961 tcaaggagtt caagagagag acaggggttg atttgaccaa agacaacatg gcgcttcaga
     1021 gggttcggga agctgctgag aaggctaaat gtgaactttc ctcatctgtg cagactgaca
     1081 tcaacttgcc ataccttacc atggatgctt ctggaccaaa gcatttgaat atgaagctga
     1141 ctcgagctca gtttgaaggc attgtcacag atctaatcaa gagaactatt gctccgtgtc
     1201 agaaagctat gcaggatgca gaagtcagca agagtgacat aggagaagtg attctggttg
     1261 gtggcatgac aaggatgccc aaggttcagc agactgtaca agatcttttt ggcagagccc
     1321 cgagtaaagc tgttaatcct gatgaggctg tagccatcgg agctgccatc cagggaggtg
     1381 tgttggctgg tgacgttaca gacgtgctgc tcctggatgt cactcccctc tctctgggta
     1441 ttgagactct gggaggcgtc tttaccaaac ttattaatag gaacaccact attccaacca
     1501 aaaagagcca ggtgttttct actgctgctg atggacaaac tcaagtagag attaaagtgt
     1561 gtcaggggga acgagagatg gctggagaca acaaacttct aggacagttc actttgattg
     1621 gaattccccc agcccctcgt ggagtgcccc agattgaagt tacatttgac attgatgcca
     1681 atgggattgt gcacgtttct gccaaagata aaggcactgg tcgtgagcaa cagattgtaa
     1741 tccagtcttc tggtggatta agcaaagatg atattgaaaa tatggttaaa aatgcagaga
     1801 agtacgctga ggaagaccgc aggaagaagg aacgtgttga agcagttaat atggctgaag
     1861 gaattattca tgacacagaa accaagatgg aagaatttaa ggaccagttg cctgctgatg
     1921 agtgcaacaa gctaaaggaa gagatttcca aaatgagagc gctccttgct ggaaaggaca
     1981 gtgagacagg agagaacatc aggcaggcag catcttccct acagcaggcg tcattgaaac
     2041 tcttcgaaat ggcgtacaaa aagatggcat ctgaacggga aggttctgga agttctggca
     2101 ctggggaaca gaaggaagat cagaaggaag agaaacagta atcgtggcag tgcattgtgg
     2161 agccagaagg acatactatg aagcttggga ctaaagggac ttcctgagca gaaaaggggc
     2221 agacttcagt ctttttactg tatttttgca gtattctata tataatttcc ttaatatata
     2281 aacttagtga caattgctaa ctcatttaat gggtaataaa gtcagcaata gcaggttcat
     2341 actgttctgt cactagcctg ttattttcag ctgcatgtaa aggggtggga tggggctgtg
     2401 aaccaatcat taaggtagat ttggtttgtg ctgaaatggc tgtgatttca aggtgggaag
     2461 cccatttcac atgcagtgga ggtagtctgt cattgacctt gagttgagat catatgcaga
     2521 tgcttgttgg ccaagagcac tactataaag aatgacctct gtatatttgc tcctacaact
     2581 gatgccttta agactgagct acctgtacca tggtctgtag gtgcagaagc taggtcagtg
     2641 gatagcagtt gtgttagcca tagcttaaag tatgatatga gaatgatata agcctctcat
     2701 gggcctgagg catacttctc tagccaccct cttggttggc caatgtctgg catctgtatt
     2761 cttgatgatt gttccttttt catccactct ggatttttta aataaaattc tgaaagcctc
     2821 ttgatctcct ttgtgaatgg tgatagctca aggattatga ctgctatcag ttttgtaggg
     2881 agaaaaatca ctggctaaaa ggttgaacaa atgaaacatg gggagtgact aataaaatgc
     2941 tggcatatat gctggatgtg aaagtccact caggaagcag tttgaagcca ggcagggctg
     3001 tacagttaga ctctgtctta ataaaagtac tggcataaac aaaaaaaaaa aaaaaaaaaa
     3061 aaaaaaa
//
