LOCUS       NM_031165               2119 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus heat shock protein 8 (Hspa8), transcript variant 1,
            mRNA.
ACCESSION   NM_031165 NR_015570
VERSION     NM_031165.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Drazic Maras,E., Kelam,N., Racetin,A., Haque,E., Drazic,M.,
            Vukojevic,K., Katsuyama,Y., Saraga-Babic,M. and Filipovic,N.
  TITLE     Autophagy markers expression pattern in developing liver of the
            yotari (dab1-/-) mice and humans
  JOURNAL   Acta Histochem 127 (1), 152224 (2025)
   PUBMED   39647211
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Gan,S., Zhou,S., Ma,J., Xiong,M., Xiong,W., Fan,X., Liu,K., Gui,Y.,
            Chen,B., Zhang,B., Wang,X., Wang,F., Li,Z., Yan,W., Ma,M. and
            Yuan,S.
  TITLE     BAG5 regulates HSPA8-mediated protein folding required for sperm
            head-tail coupling apparatus assembly
  JOURNAL   EMBO Rep 25 (4), 2045-2070 (2024)
   PUBMED   38454159
  REMARK    GeneRIF: BAG5 regulates HSPA8-mediated protein folding required for
            sperm head-tail coupling apparatus assembly.
REFERENCE   3  (bases 1 to 2119)
  AUTHORS   Hu,L., Tang,D., Qi,B., Guo,D., Wang,Y., Geng,J., Zhang,X., Song,L.,
            Chang,P., Chen,W., Fu,F. and Li,Y.
  TITLE     Mfn2/Hsc70 Complex Mediates the Formation of Mitochondria-Lipid
            Droplets Membrane Contact and Regulates Myocardial Lipid Metabolism
  JOURNAL   Adv Sci (Weinh) 11 (14), e2307749 (2024)
   PUBMED   38311582
  REMARK    GeneRIF: Mfn2/Hsc70 Complex Mediates the Formation of
            Mitochondria-Lipid Droplets Membrane Contact and Regulates
            Myocardial Lipid Metabolism.
REFERENCE   4  (bases 1 to 2119)
  AUTHORS   Li,B., Ming,H., Qin,S., Zhou,L., Huang,Z., Jin,P., Peng,L., Luo,M.,
            Zhang,T., Wang,K., Liu,R., Liou,Y.C., Nice,E.C., Jiang,J. and
            Huang,C.
  TITLE     HSPA8 Activates Wnt/beta-Catenin Signaling to Facilitate BRAF V600E
            Colorectal Cancer Progression by CMA-Mediated CAV1 Degradation
  JOURNAL   Adv Sci (Weinh) 11 (3), e2306535 (2024)
   PUBMED   37973552
  REMARK    GeneRIF: HSPA8 Activates Wnt/beta-Catenin Signaling to Facilitate
            BRAF V600E Colorectal Cancer Progression by CMA-Mediated CAV1
            Degradation.
REFERENCE   5  (bases 1 to 2119)
  AUTHORS   Subramanian,M., Mills,W.T. 4th, Paranjpe,M.D., Onuchukwu,U.S.,
            Inamdar,M., Maytin,A.R., Li,X., Pomerantz,J.L. and Meffert,M.K.
  TITLE     Growth-suppressor microRNAs mediate synaptic overgrowth and
            behavioral deficits in Fragile X mental retardation protein
            deficiency
  JOURNAL   iScience 27 (1), 108676 (2023)
   PUBMED   38235335
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2119)
  AUTHORS   Leverette,R.D., Andrews,M.T. and Maxwell,E.S.
  TITLE     Mouse U14 snRNA is a processed intron of the cognate hsc70 heat
            shock pre-messenger RNA
  JOURNAL   Cell 71 (7), 1215-1221 (1992)
   PUBMED   1282084
REFERENCE   7  (bases 1 to 2119)
  AUTHORS   Shanab,G.M. and Maxwell,E.S.
  TITLE     Determination of the nucleotide sequences in mouse U14 small
            nuclear RNA and 18S ribosomal RNA responsible for in vitro
            intermolecular base-pairing
  JOURNAL   Eur J Biochem 206 (2), 391-400 (1992)
   PUBMED   1375913
REFERENCE   8  (bases 1 to 2119)
  AUTHORS   Manejwala,F.M., Logan,C.Y. and Schultz,R.M.
  TITLE     Regulation of hsp70 mRNA levels during oocyte maturation and
            zygotic gene activation in the mouse
  JOURNAL   Dev Biol 144 (2), 301-308 (1991)
   PUBMED   2010034
  REMARK    Erratum:[Dev Biol 1991 Nov;148(1):402]
REFERENCE   9  (bases 1 to 2119)
  AUTHORS   Liu,J. and Maxwell,E.S.
  TITLE     Mouse U14 snRNA is encoded in an intron of the mouse cognate hsc70
            heat shock gene
  JOURNAL   Nucleic Acids Res 18 (22), 6565-6571 (1990)
   PUBMED   2251119
REFERENCE   10 (bases 1 to 2119)
  AUTHORS   Maxwell,E.S. and Martin,T.E.
  TITLE     A low-molecular-weight RNA from mouse ascites cells that hybridizes
            to both 18S rRNA and mRNA sequences
  JOURNAL   Proc Natl Acad Sci U S A 83 (19), 7261-7265 (1986)
   PUBMED   2429302
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK151775.1 and CD774246.1.
            
            On Jun 19, 2018 this sequence version replaced NM_031165.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.212272.1, BC085486.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2101              AK151775.1         4-2104
            2102-2119           CD774246.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 21.55 cM"
     gene            1..2119
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /note="heat shock protein 8"
                     /db_xref="GeneID:15481"
                     /db_xref="MGI:MGI:105384"
     exon            1..73
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            74..283
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     CDS             79..2019
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /EC_number="3.6.4.10"
                     /note="isoform 1 is encoded by transcript variant 1; heat
                     shock protein cognate 70; heat shock cognate 71 kDa
                     protein; heat shock cognate hsc73; heat shock 70 kDa
                     protein 8"
                     /codon_start=1
                     /product="heat shock cognate 71 kDa protein isoform 1"
                     /protein_id="NP_112442.2"
                     /db_xref="CCDS:CCDS23083.1"
                     /db_xref="GeneID:15481"
                     /db_xref="MGI:MGI:105384"
                     /translation="MSKGPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAF
                     TDTERLIGDAAKNQVAMNPTNTVFDAKRLIGRRFDDAVVQSDMKHWPFMVVNDAGRPK
                     VQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGKTVTNAVVTVPAYFNDSQRQATKD
                     AGTIAGLNVLRIINEPTAAAIAYGLDKKVGAERNVLIFDLGGGTFDVSILTIEDGIFE
                     VKSTAGDTHLGGEDFDNRMVNHFIAEFKRKHKKDISENKRAVRRLRTACERAKRTLSS
                     STQASIEIDSLYEGIDFYTSITRARFEELNADLFRGTLDPVEKALRDAKLDKSQIHDI
                     VLVGGSTRIPKIQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAILSGDKSENVQDLL
                     LLDVTPLSLGIETAGGVMTVLIKRNTTIPTKQTQTFTTYSDNQPGVLIQVYEGERAMT
                     KDNNLLGKFELTGIPPAPRGVPQIEVTFDIDANGILNVSAVDKSTGKENKITITNDKG
                     RLSKEDIERMVQEAEKYKAEDEKQRDKVSSKNSLESYAFNMKATVEDEKLQGKINDED
                     KQKILDKCNEIISWLDKNQTAEKEEFEHQQKELEKVCNPIITKLYQSAGGMPGGMPGG
                     FPGGGAPPSGGASSGPTIEEVD"
     exon            284..489
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            490..642
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            643..1198
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1401
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1600
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            1601..1833
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..2104
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2078..2083
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /note="hexamer: AATAAA"
     polyA_site      2099
                     /gene="Hspa8"
                     /gene_synonym="2410008N15Rik; Hsc70; Hsc71; Hsc73; Hsp73;
                     Hspa10"
                     /note="major polyA site"
ORIGIN      
        1 ctcattgaac gcggaggcag ctgcctggca tttgtgtggt ctcgtcgtca gcgcagctgg
       61 gcctacacac aagcaaccat gtctaaggga cctgcagttg gcattgatct cggcaccacc
      121 tactcctgtg tgggtgtctt ccagcatgga aaggtggaaa ttattgccaa tgaccagggt
      181 aaccgcacca cgccaagcta tgttgctttc acggacacag agagattaat tggggatgcg
      241 gccaagaatc aggttgcaat gaaccccacc aacacagttt ttgatgccaa acgtctgatc
      301 gggcgtaggt ttgatgatgc tgttgttcag tctgatatga agcactggcc cttcatggtg
      361 gtgaatgatg caggcaggcc caaggtccaa gtggaataca aaggggagac aaaaagtttc
      421 tacccagagg aagtgtcctc catggttctg acaaagatga aggaaattgc agaagcgtac
      481 ctcggaaaga ccgttaccaa cgctgtggtc acagtgcccg cttacttcaa tgactctcag
      541 cgacaggcaa caaaagatgc tggaactatt gctggcctca atgtacttcg aatcatcaat
      601 gaaccaactg ctgctgctat tgcttacggc ttagataaga aggtcggagc tgaaaggaat
      661 gtgctcattt ttgacttggg aggtggcact tttgatgtgt caatcctcac tattgaggat
      721 ggaatttttg aggtcaaatc aacagctgga gacacccact taggtggaga agattttgac
      781 aaccgaatgg tcaatcattt cattgctgag ttcaagcgaa agcacaagaa agacatcagt
      841 gagaacaaga gagctgtccg ccgtctccgc acggcctgcg agcgggccaa gcgcaccctc
      901 tcctccagca cccaggccag tattgagatt gattctctct atgagggaat tgacttctat
      961 acctccatta cccgggctcg atttgaggag ttgaatgctg acctgttccg tggcacactg
     1021 gaccctgtag agaaggccct tcgagatgcc aagctggaca agtcacagat ccatgatatt
     1081 gtcttggtgg gtggttctac cagaatcccc aagattcaga aacttctgca agacttcttc
     1141 aatggaaaag agctgaacaa gagcattaac cccgatgaag ctgttgccta tggtgcagct
     1201 gtccaggcag ccattctatc tggagacaag tctgagaacg ttcaggattt gctgctcttg
     1261 gatgtcactc ctctttccct tggtattgaa actgctggcg gagtcatgac tgtcctcatc
     1321 aagcgcaata ccaccatccc caccaagcag acacagactt tcaccaccta ctctgacaac
     1381 cagcctggtg tactcattca ggtgtatgaa ggtgaaaggg ccatgaccaa ggacaacaac
     1441 ctgcttggaa agttcgagct cacaggcatc cctccagcac cccgtggggt ccctcagatt
     1501 gaggttactt ttgacatcga tgccaatggc atcctcaatg tttctgctgt agataagagc
     1561 acaggaaagg agaacaagat caccatcacc aatgacaagg gccgcttgag taaggaagat
     1621 attgagcgca tggtccaaga agctgagaag tacaaggctg aggatgagaa gcagagagat
     1681 aaggtttcct ccaagaactc actggagtcc tatgccttca acatgaaagc aactgtggaa
     1741 gatgagaaac ttcaaggcaa gatcaatgat gaggacaaac agaagattct tgacaagtgc
     1801 aatgaaatca tcagctggct ggataagaac cagactgcag agaaggaaga atttgagcat
     1861 cagcagaaag aactggagaa agtctgcaac cctatcatta ccaagctgta ccagagtgca
     1921 ggtggcatgc ctgggggaat gcctggtggc ttcccaggtg gaggagctcc cccatctggt
     1981 ggtgcttctt caggccccac cattgaagag gtggattaag tcagtccaag aaggtgtagc
     2041 tttgttccac agggacccaa aacaagtaac atggaataat aaaactattt aaattggcac
     2101 cataaaaaaa aaaaaaaaa
//
