LOCUS       NM_001163434            2534 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus heat shock protein 5 (Hspa5), transcript variant 1,
            mRNA.
ACCESSION   NM_001163434
VERSION     NM_001163434.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Drazic Maras,E., Kelam,N., Racetin,A., Haque,E., Drazic,M.,
            Vukojevic,K., Katsuyama,Y., Saraga-Babic,M. and Filipovic,N.
  TITLE     Autophagy markers expression pattern in developing liver of the
            yotari (dab1-/-) mice and humans
  JOURNAL   Acta Histochem 127 (1), 152224 (2025)
   PUBMED   39647211
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Xie,D., Ma,Y., Gao,C. and Pan,S.
  TITLE     Piezo1 activation on microglial cells exacerbates demyelination in
            sepsis by influencing the CCL25/GRP78 pathway
  JOURNAL   Int Immunopharmacol 142 (Pt A), 113045 (2024)
   PUBMED   39236454
  REMARK    GeneRIF: Piezo1 activation on microglial cells exacerbates
            demyelination in sepsis by influencing the CCL25/GRP78 pathway.
REFERENCE   3  (bases 1 to 2534)
  AUTHORS   Nedomova,M., Haberecht-Muller,S., Moller,S., Venz,S.,
            Prochazkova,M., Prochazka,J., Sedlak,F., Chawengsaksophak,K.,
            Hammer,E., Kasparek,P., Adamek,M., Sedlacek,R., Konvalinka,J.,
            Kruger,E. and Grantz Saskova,K.
  TITLE     DDI2 protease controls embryonic development and inflammation via
            TCF11/NRF1
  JOURNAL   iScience 27 (10), 110893 (2024)
   PUBMED   39328932
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2534)
  AUTHORS   Kot,A., Chun,C., Martin,J.H., Wachtell,D., Hudson,D., Weis,M.,
            Marks,H., Srivastava,S., Eyre,D.R., Duran,I., Zieba,J. and
            Krakow,D.
  TITLE     Loss of the long form of Plod2 phenocopies contractures of Bruck
            syndrome-osteogenesis imperfecta
  JOURNAL   J Bone Miner Res 39 (9), 1240-1252 (2024)
   PUBMED   39088537
REFERENCE   5  (bases 1 to 2534)
  AUTHORS   Borisova,E., Newman,A.G., Couce Iglesias,M., Dannenberg,R.,
            Schaub,T., Qin,B., Rusanova,A., Brockmann,M., Koch,J., Daniels,M.,
            Turko,P., Jahn,O., Kaplan,D.R., Rosario,M., Iwawaki,T.,
            Spahn,C.M.T., Rosenmund,C., Meierhofer,D., Kraushar,M.L.,
            Tarabykin,V. and Ambrozkiewicz,M.C.
  TITLE     Protein translation rate determines neocortical neuron fate
  JOURNAL   Nat Commun 15 (1), 4879 (2024)
   PUBMED   38849354
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2534)
  AUTHORS   Haas,I.G., Simon-Chazottes,D. and Guenet,J.L.
  TITLE     The gene coding for the immunoglobulin heavy chain binding protein
            BiP (Hsce-70) maps to mouse chromosome 2
  JOURNAL   Mamm Genome 3 (11), 659-660 (1992)
   PUBMED   1450517
REFERENCE   7  (bases 1 to 2534)
  AUTHORS   Gaskins,H.R., Prochazka,M. and Leiter,E.H.
  TITLE     Chromosomal localization of the murine stress protein gene encoding
            glucose-regulated protein 78 (BiP)
  JOURNAL   Mamm Genome 3 (12), 709-711 (1992)
   PUBMED   1362103
REFERENCE   8  (bases 1 to 2534)
  AUTHORS   Kim,S.K., Kim,Y.K. and Lee,A.S.
  TITLE     Expression of the glucose-regulated proteins (GRP94 and GRP78) in
            differentiated and undifferentiated mouse embryonic cells and the
            use of the GRP78 promoter as an expression system in embryonic
            cells
  JOURNAL   Differentiation 42 (3), 153-159 (1990)
   PUBMED   1692794
REFERENCE   9  (bases 1 to 2534)
  AUTHORS   Parfett,C.L., Hofbauer,R., Brudzynski,K., Edwards,D.R. and
            Denhardt,D.T.
  TITLE     Differential screening of a cDNA library with cDNA probes amplified
            in a heterologous host: isolation of murine GRP78 (BiP) and other
            serum-regulated low-abundance mRNAs
  JOURNAL   Gene 82 (2), 291-303 (1989)
   PUBMED   2583523
REFERENCE   10 (bases 1 to 2534)
  AUTHORS   Kozutsumi,Y., Normington,K., Press,E., Slaughter,C., Sambrook,J.
            and Gething,M.J.
  TITLE     Identification of immunoglobulin heavy chain binding protein as
            glucose-regulated protein 78 on the basis of amino acid sequence,
            immunological cross-reactivity, and functional activity
  JOURNAL   J Cell Sci Suppl 11, 115-137 (1989)
   PUBMED   2559088
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL929106.5 and AK148539.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK151647.1, AK148539.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AL929106.5         63570-63571
            3-2470              AK148539.1         2-2469
            2471-2534           AL929106.5         67946-68009
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 22.94 cM"
     gene            1..2534
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /note="heat shock protein 5"
                     /db_xref="GeneID:14828"
                     /db_xref="MGI:MGI:95835"
     exon            1..57
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            58..275
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..2118
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /EC_number="3.6.4.10"
                     /note="78 kDa glucose-regulated protein; GRP-78; heat
                     shock 70 kDa protein 5; immunoglobulin heavy chain-binding
                     protein; glucose regulated protein, 78 kDa; XAP-1 antigen;
                     heat shock 70kD protein 5 (glucose-regulated protein,
                     78kD); HSP70 family protein 5; binding-immunoglobulin
                     protein; heat shock protein family A member 5; heat shock
                     protein 70 family protein 5; endoplasmic reticulum
                     chaperone BiP"
                     /codon_start=1
                     /product="endoplasmic reticulum chaperone BiP precursor"
                     /protein_id="NP_001156906.1"
                     /db_xref="CCDS:CCDS15950.1"
                     /db_xref="GeneID:14828"
                     /db_xref="MGI:MGI:95835"
                     /translation="MMKFTVVAAALLLLGAVRAEEEDKKEDVGTVVGIDLGTTYSCVG
                     VFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGR
                     TWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEA
                     YLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGE
                     KNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTG
                     KDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMD
                     LFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPD
                     EAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQ
                     IFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNG
                     ILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNEL
                     ESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKEL
                     EEIVQPIISKLYGSGGPPPTGEEDTSEKDEL"
     exon            276..507
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            508..645
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            646..758
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            759..1149
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1387
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1555
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..2534
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2443..2448
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /note="hexamer: AATAAA"
     polyA_site      2461
                     /gene="Hspa5"
                     /gene_synonym="baffled; Bip; D2Wsu141e; D2Wsu17e; Grp78;
                     Hsce70; mBiP; SEZ-7; Sez7"
                     /note="major polyA site"
ORIGIN      
        1 tcgatactgg ccgagacaac actgacctgg acacttgggc ttctgcgtgt gtgtgagacc
       61 agaaccgtcg tcgcgtttcg gggccacagc ctgttgctgg actcctaaga ctcctgcctg
      121 actgctgagc gactggtcct cagcgccggc atgatgaagt tcactgtggt ggcggcggcg
      181 ttgctgctgc tgggcgcggt gcgggccgag gaggaggaca agaaggagga tgtgggcacg
      241 gtggtcggca tcgacttggg gaccacctat tcctgcgtcg gtgtgttcaa gaacggccgc
      301 gtggagatca tagccaacga tcagggcaac cgcatcacgc cgtcgtatgt ggccttcact
      361 cctgaagggg agcgtctgat tggcgatgcg gccaagaacc aactcacgtc caaccccgag
      421 aacacggtct tcgatgccaa gcgcctcatc ggacgcactt ggaatgaccc ttcggtgcag
      481 caggacatca agttcttgcc attcaaggtg gttgaaaaga aaactaaacc gtacattcaa
      541 gttgatattg gaggtgggca aaccaagaca tttgccccag aagaaatttc tgccatggtt
      601 ctcactaaaa tgaaggagac tgctgaggcg tatttgggaa agaaggttac ccatgcagtt
      661 gttactgtac cagcttactt caatgatgcc cagcgacaag caaccaaaga tgctggcact
      721 attgctggac tgaatgtcat gaggatcatc aatgagccta cagcagctgc tattgcatat
      781 ggcctggata agagagaggg agagaagaac atccttgtgt ttgacctggg tggcggcacc
      841 ttcgatgtgt ctcttctcac cattgacaat ggtgtctttg aagtggtggc cactaatgga
      901 gatactcacc tgggtgggga agactttgat cagcgggtca tggaacactt catcaagttg
      961 tacaaaaaga aaactggtaa agatgttagg aaagacaaca gagctgtgca gaaactccgg
     1021 cgtgaggtag aaaaggctaa gagagccttg tcttctcagc atcaagcaag gattgaaatt
     1081 gagtccttct tcgaaggaga agacttctca gagaccctta ctcgggccaa atttgaagag
     1141 ctgaacatgg acctgttccg ctctaccatg aagcctgtcc agaaagtgtt ggaagactct
     1201 gatctgaaga aatctgatat tgatgaaatt gttctggttg gtggatctac tcgaattcca
     1261 aagattcagc aactggtgaa agagttcttc aatggcaagg agccatcccg tggcataaac
     1321 cccgatgagg ctgtagccta tggtgccgct gtccaggctg gtgtcctctc tggtgatcag
     1381 gatacaggtg atctggtact gcttgatgtt tgtcccctta cacttggtat tgaaactgtg
     1441 ggaggagtca tgacaaaact gattccaagg aacactgtgg tacccaccaa gaagtctcag
     1501 atcttctcca cggcttccga taatcagcca actgtaacaa tcaaggtcta tgaaggtgaa
     1561 cgacccctaa caaaagacaa tcatcttctg ggtacatttg atctgactgg aattcctcct
     1621 gctccccgtg gagttcccca gattgaagtc acttttgaga tagatgttaa tggtattctc
     1681 cgagtgacag ctgaagacaa aggtacagga aacaaaaaca aaatcacaat taccaatgac
     1741 caaaaccgcc tgacacctga agaaattgaa aggatggtta atgatgctga gaagtttgct
     1801 gaggaagaca aaaagctcaa agagcgcatt gacaccagga atgaattgga aagctatgct
     1861 tattctctca agaaccagat tggagataaa gaaaagctgg gaggtaaact ttcttctgaa
     1921 gataaagaaa ccatggaaaa agctgtagag gaaaagattg aatggctgga aagccaccag
     1981 gatgcggaca ttgaagactt taaagccaaa aagaaggaac tagaagaaat tgttcagcca
     2041 attatcagca aactctatgg aagtggaggc cctcccccaa ctggtgaaga ggatacatca
     2101 gaaaaagatg agttgtaggt gcactgatct gctagagctg taatattgta aatactggac
     2161 tcaggaactt tcgttgggag aaaattgaga gaacttaagt ctcgaatgta attggaatct
     2221 tcacctcaga gtggagttga aaatgctata gcccaagtgg ctgtttactg cttttcatta
     2281 gcagttgctc acatgtcttg gggtcaggga gaggaggaat tggctatttt aaaaattgag
     2341 aaaaaggtgg gtcagggtgt gtgttcacct tggatatggt ctatttaaca attgggtcat
     2401 gcacatgtgg tgtaggaact tttttctacc ataagtgaca ccaataaatg tttgttattt
     2461 acactggtct aatttttgtg aaaagtttgt aattatatcc atcttttatt ttagataact
     2521 aaaatttaag actg
//
