LOCUS       NM_001410463            9852 bp    mRNA    linear   ROD 13-JUN-2024
DEFINITION  Mus musculus heat shock protein 4 like (Hspa4l), transcript variant
            2, mRNA.
ACCESSION   NM_001410463 XM_006500766
VERSION     NM_001410463.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  AUTHORS   Nagahori,K., Hirai,S., Hatayama,N., Kuramasu,M., Omotehara,T.,
            Kawata,S., Li,Z., Miyaso,H., Ogawa,Y., Qu,N., Terayama,H.,
            Hayashi,S., Yi,S.Q., Naito,M. and Itoh,M.
  TITLE     Heat shock protein A4L is a potent autoantigen for testicular
            autoimmunity in mice
  JOURNAL   J Reprod Immunol 145, 103318 (2021)
   PUBMED   33894646
  REMARK    GeneRIF: Heat shock protein A4L is a potent autoantigen for
            testicular autoimmunity in mice.
REFERENCE   3  (bases 1 to 9852)
  AUTHORS   Itoh,K., Kondoh,G., Miyachi,H., Sugai,M., Kaneko,Y., Kitano,S.,
            Watanabe,H., Maeda,R., Imura,A., Liu,Y., Ito,C., Itohara,S.,
            Toshimori,K. and Fujita,J.
  TITLE     Dephosphorylation of protamine 2 at serine 56 is crucial for murine
            sperm maturation in vivo
  JOURNAL   Sci Signal 12 (574) (2019)
   PUBMED   30914484
  REMARK    GeneRIF: Hspa4l-null mice showed male infertility and the
            malformation of sperm heads. Hspa4l enhanced the release of Ppp1cc2
            from complexes with Hsp70 and Hsc70. Introduction of a Prm2 S56A
            mutation to Hspa4l-null mice restored the malformation of sperm
            heads and the infertility of Hspa4l (-/-) mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9852)
  AUTHORS   Nagy,M., Fenton,W.A., Li,D., Furtak,K. and Horwich,A.L.
  TITLE     Extended survival of misfolded G85R SOD1-linked ALS mice by
            transgenic expression of chaperone Hsp110
  JOURNAL   Proc Natl Acad Sci U S A 113 (19), 5424-5428 (2016)
   PUBMED   27114530
REFERENCE   5  (bases 1 to 9852)
  AUTHORS   Mohamed,B.A., Barakat,A.Z., Held,T., Elkenani,M., Muhlfeld,C.,
            Manner,J. and Adham,I.M.
  TITLE     Respiratory distress and early neonatal lethality in Hspa4l/Hspa4
            double-mutant mice
  JOURNAL   Am J Respir Cell Mol Biol 50 (4), 817-824 (2014)
   PUBMED   23980576
  REMARK    GeneRIF: HSPA4L and HSPA4 collaborate in embryonic lung maturation,
            which is necessary for adaptation to air breathing at birth.
REFERENCE   6  (bases 1 to 9852)
  AUTHORS   Held,T., Paprotta,I., Khulan,J., Hemmerlein,B., Binder,L., Wolf,S.,
            Schubert,S., Meinhardt,A., Engel,W. and Adham,I.M.
  TITLE     Hspa4l-deficient mice display increased incidence of male
            infertility and hydronephrosis development
  JOURNAL   Mol Cell Biol 26 (21), 8099-8108 (2006)
   PUBMED   16923965
  REMARK    GeneRIF: These results provide evidence that Hspa4l is required for
            normal spermatogenesis and suggest that Hspa4l plays a role in
            osmotolerance.
REFERENCE   7  (bases 1 to 9852)
  AUTHORS   Kojima,R., Randall,J.D., Ito,E., Manshio,H., Suzuki,Y. and
            Gullans,S.R.
  TITLE     Regulation of expression of the stress response gene, Osp94:
            identification of the tonicity response element and intracellular
            signalling pathways
  JOURNAL   Biochem J 380 (Pt 3), 783-794 (2004)
   PUBMED   15018608
REFERENCE   8  (bases 1 to 9852)
  AUTHORS   Kaneko,Y., Kimura,T., Nishiyama,H., Noda,Y. and Fujita,J.
  TITLE     Developmentally regulated expression of APG-1, a member of heat
            shock protein 110 family in murine male germ cells
  JOURNAL   Biochem Biophys Res Commun 233 (1), 113-116 (1997)
   PUBMED   9144406
REFERENCE   9  (bases 1 to 9852)
  AUTHORS   Kaneko,Y., Nishiyama,H., Nonoguchi,K., Higashitsuji,H.,
            Kishishita,M. and Fujita,J.
  TITLE     A novel hsp110-related gene, apg-1, that is abundantly expressed in
            the testis responds to a low temperature heat shock rather than the
            traditional elevated temperatures
  JOURNAL   J Biol Chem 272 (5), 2640-2645 (1997)
   PUBMED   9006898
REFERENCE   10 (bases 1 to 9852)
  AUTHORS   Kojima,R., Randall,J., Brenner,B.M. and Gullans,S.R.
  TITLE     Osmotic stress protein 94 (Osp94). A new member of the Hsp110/SSE
            gene subfamily
  JOURNAL   J Biol Chem 271 (21), 12327-12332 (1996)
   PUBMED   8647834
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160757.3.
            
            On Aug 8, 2022 this sequence version replaced XM_006500766.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13948564.2883708.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-554               AC160757.3         199862-200415       c
            555-728             AC160757.3         198863-199036       c
            729-786             AC160757.3         193915-193972       c
            787-927             AC160757.3         191357-191497       c
            928-1050            AC160757.3         187524-187646       c
            1051-1150           AC160757.3         184184-184283       c
            1151-1284           AC160757.3         183732-183865       c
            1285-1529           AC160757.3         177591-177835       c
            1530-1606           AC160757.3         177368-177444       c
            1607-1758           AC160757.3         176526-176677       c
            1759-1865           AC160757.3         175789-175895       c
            1866-1999           AC160757.3         174003-174136       c
            2000-2196           AC160757.3         171904-172100       c
            2197-2295           AC160757.3         165214-165312       c
            2296-2433           AC160757.3         162950-163087       c
            2434-2559           AC160757.3         160293-160418       c
            2560-2667           AC160757.3         159920-160027       c
            2668-2787           AC160757.3         159249-159368       c
            2788-2949           AC160757.3         157762-157923       c
            2950-9852           AC160757.3         148541-155443       c
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 19.51 cM"
     gene            1..9852
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /note="heat shock protein 4 like"
                     /db_xref="GeneID:18415"
                     /db_xref="MGI:MGI:107422"
     exon            1..554
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            555..728
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     CDS             622..3138
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /note="isoform 1 is encoded by transcript variant 2; heat
                     shock 70kDa protein 4 like; heat shock 70 kDa protein 4L;
                     osmotic stress protein 94; heat shock 70-related protein
                     APG-1"
                     /codon_start=1
                     /product="heat shock 70 kDa protein 4L isoform 1"
                     /protein_id="NP_001397392.1"
                     /db_xref="GeneID:18415"
                     /db_xref="MGI:MGI:107422"
                     /translation="MSVVGIDLGFLNCYIAVARSGGIETIANEYSDRCTPACISLGSR
                     TRAIGNAAKSQIVTNVRNTIHGFKKLHGRSFDDPIVQTERIRLPYELQKMPNGSTGVK
                     VRYLEEERPFAIEQVTGMLLAKLKETSENALKKPVADCVISIPSFFTDAERRSVMAAA
                     QVAGLNCLRLMNETTAVALAYGIYKQDLPSLDEKPRNVVFIDMGHSAYQVSVCAFNKG
                     KLKVLATTFDPYLGGRNFDEALVDYFCDEFKTKYKINVKENSRALLRLYQECEKLKKL
                     MSANASDLPLNIECFMNDLDVSSKMNRAQFEQLCASLLARVEPPLKSVMDQANLQRED
                     INSIEIVGGATRIPAVKEQVTRFFLKDISTTLNADEAVARGCALQCAILSPAFKVREF
                     SITDLVPYSVTLRWKTSFEEGTGECEVFSKNHPAPFSKVITFHKKEPFELEAFYTNLH
                     EVPYPDPRIGNFTIQNVFPQSDGDSSKVKVKVRINIHGIFSVASASVIEKQNLEGDHN
                     DAAMETEAPKSEGKEDVDKMQVDQEEGGHQKCHAEHTPEEEIDHTGAKAKAPPSDKQD
                     RINQTIKKGKIKSIDLPIQSSLYRQLTQDLLNSYIENEGKMIMQDKLEKERNDAKNAV
                     EEYVYDFRDKLGTVYEKFITPEDMNKLSAMLEDTENWLYEEGEDQPKQVYVDRLQELK
                     KYGQPIQMKYVEHEERPKALNDLGKKIQLVLKVIEAHRNKDERYDHLDPAEMERVEKY
                     ISDSMNWLNSKMNAQNKLSLTQDPVVKVSEIVTKSKELDNFCNPIVYKPKPKVEAPED
                     KAKTGSEHNGPMDGQSGSETSPDPPKGSSQHTDSGEMEVD"
     exon            729..786
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            787..927
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            928..1050
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1150
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1151..1284
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1529
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1606
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1758
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1865
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1999
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2196
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2295
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2296..2433
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2434..2559
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2560..2667
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2787
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2788..2949
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..9852
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4166..4171
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /note="hexamer: ATTATA"
     polyA_site      4186
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /note="major polyA site"
     regulatory      9827..9832
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /note="hexamer: AATACA"
     polyA_site      9852
                     /gene="Hspa4l"
                     /gene_synonym="94kDa; APG-1; Osp94"
                     /note="major polyA site"
ORIGIN      
        1 aaggtaggtg gtctcctaag aaagtgctga gtgggtcaga gcatgccccc tgcagactct
       61 gaagtcaaaa aagattcttt agagacacta gagttggaac aatagtggcg tcccagttga
      121 cttggtatag ttcctaaaat gtattttgta ttcattatag caacagcggg ggctattgcc
      181 acacttgctg ctgccgccct aacagcagtg ttaatagtga agcgattgag taacttaaga
      241 aggagcacaa atacggacta tgagagtact gaatatgcgg ataaaaattc cgtgtcaaat
      301 tccaaggtga aacaggaagc acacaaaaac ttggacgagc tggaagaggg attacacaag
      361 gaattggatg agaagggtca ccagacacag gaactggacg agaagggtca gaatgtacat
      421 gaagaactag ataagaagag cccacagata catgaagaac tggaccctaa ggaacgtggg
      481 acacaggggc tggataaaaa agagccaaat tcacactggg atgtgcatgg aaaggaccag
      541 gaccgccaga cttcgagtcg cagcgcccgc agtggggacc cgcagcggga cgcggtctcc
      601 gcagcccgag ccttaagcgg gatgtcggtg gtgggcattg acctcggctt cctcaactgc
      661 tacatcgctg tagcgaggag cggcggcatc gagaccatcg ccaacgagta cagcgacagg
      721 tgcacgccgg cctgtatatc tttgggatcc agaactcgag ccattggaaa tgcagctaag
      781 agccagatag tcacaaatgt aagaaataca attcatggct tcaaaaagct tcatgggcga
      841 tcatttgatg accccattgt gcaaacggag aggatcaggc ttccgtacga gctgcagaag
      901 atgcctaatg gaagtacagg tgttaaggtg cggtacctgg aagaagagcg gccctttgca
      961 attgagcaag tcactgggat gttgctggct aagcttaaag agacctcaga aaatgctctg
     1021 aagaagccag tggctgactg tgtgatctcg atcccgagct ttttcaccga cgcagagaga
     1081 agatccgtga tggccgcagc ccaggttgca ggcctaaact gtctgaggct gatgaatgaa
     1141 accactgcag ttgcactggc atatggaatt tataagcagg atcttccctc attagatgag
     1201 aaaccaagga atgttgtgtt tatcgacatg ggacattctg cctaccaggt ctctgtttgt
     1261 gcttttaaca aaggaaaact gaaagtgttg gctactacct ttgacccata tttgggtggc
     1321 aggaactttg atgaggcttt agtagactac ttctgcgatg aattcaagac caaatataag
     1381 ataaatgtca aagagaactc gcgggccttg ttgcggctgt atcaggagtg tgaaaaacta
     1441 aagaagctga tgagtgcaaa cgcgtcagac cttcccctga acatcgagtg tttcatgaat
     1501 gaccttgatg tttctagtaa gatgaacagg gctcaatttg agcagttgtg tgcttccctc
     1561 ttagccaggg ttgaaccacc tttaaaatca gtaatggatc aagctaactt acaacgtgaa
     1621 gacataaaca gcatagagat tgtgggaggg gccacacgga ttcctgcagt caaggagcag
     1681 gtgactaggt tctttctgaa agacatcagt accaccctga atgctgatga agctgtcgcc
     1741 cgaggatgtg cgttgcagtg tgcgattctc tcaccagcat ttaaagtacg tgaattttcc
     1801 ataactgacc ttgttcctta ctcagtcaca ttaaggtgga agacttcttt tgaagaaggg
     1861 actggggaat gtgaagtctt ctctaagaac cacccggccc cattctcaaa ggtcataact
     1921 ttccacaaga aggaaccatt tgaactagaa gcattttata ctaatttgca tgaagtgcct
     1981 tatcctgatc caagaattgg aaacttcact attcagaatg ttttcccaca gtctgatggt
     2041 gacagttcta aagtaaaagt taaagttcgt attaatatcc atggaatctt cagtgtggcc
     2101 agtgcgtcag taattgagaa gcagaatctg gaaggtgatc ataacgatgc cgctatggag
     2161 acggaagctc ctaagagtga aggcaaagag gatgtggaca aaatgcaggt tgaccaagaa
     2221 gaaggaggtc atcagaaatg tcatgctgag cacaccccag aagaggagat tgaccacacc
     2281 ggggccaaag caaaggcacc tccttcagat aagcaagatc gcataaatca aactattaaa
     2341 aaagggaaaa tcaagagtat tgatctacct atccagagta gcctctacag acagctgact
     2401 caagaccttc tcaatagtta cattgaaaat gaggggaaga tgataatgca ggataaatta
     2461 gagaaagaaa gaaatgatgc taaaaatgct gttgaagaat acgtctatga tttcagagac
     2521 aaattgggca ctgtctacga aaagttcatc actccagaag acatgaataa gctgtctgca
     2581 atgttagaag acacagaaaa ttggctgtat gaagaaggag aagaccagcc taaacaagtt
     2641 tatgtggata ggctgcagga attaaagaaa tatggccagc ccattcaaat gaagtacgtg
     2701 gagcatgaag agagaccaaa agctttaaat gacttgggga aaaagattca gcttgtcctg
     2761 aaagtgatag aagcacacag aaacaaggat gaaagatatg atcatctgga tcctgctgaa
     2821 atggaaagag ttgaaaagta catcagtgac tccatgaact ggctaaacag taagatgaat
     2881 gcacagaata aattaagtct cactcaagat cccgtggtaa aagtgtcaga aatagttaca
     2941 aagtcaaagg aactggataa tttctgtaac cccatcgttt ataagcccaa accaaaagta
     3001 gaagctcctg aagacaaagc aaaaactggt agtgagcaca atggaccaat ggacggacag
     3061 agtggttcag agaccagccc agatccaccc aaaggaagct cacagcacac cgactccgga
     3121 gagatggaag tggactaagt gtcatgttat ccaagcagtg ggttaactaa agggcccatt
     3181 catcctttat gcccggtaca cacaacatat gttcagttgt tcttaactac ttttgtcatt
     3241 tgttttttgg agtagttttg aaaagtgtct atattgagta cactattgct gtccattgct
     3301 gctgtgaagc cctagctgaa tatagatgta caaatcagtt tgagctctcc ttacatactt
     3361 tgtgtcagta tgcaggactg ctatgtactc ggcaaccatc cactgtagtc acagcttcta
     3421 cttgcatagc cctcagctcc tcgatgcagg aagggggcac tgcattgctc ttttgttgca
     3481 gaggcagggg gtaactgcat tattattgtg aaacaaaccg atgtggttta gagccaccag
     3541 cactgaccct ttcctagttt ccctaatgct atcacttaaa tattacaaca aatgggaggt
     3601 tctgacatcc tagaccatct cactgatgcg ccaccaggaa tccagtcttg tcattacata
     3661 aataatctaa atgacactca gagagggaca gtgcagtttg acattcagat aacagcagca
     3721 ttggctgctt ttgttggtag catggattca tgttttctca gaggaacttt tcacttgaaa
     3781 ctgttgatct acttgtccaa aagaccagct gctgcgacac taatgctaag cctatgggga
     3841 agcactgttg acctgctcag gtgcacatct gtgatgccga cagacttggt tcagttttta
     3901 tagttgtgtt ggttttctta attgattttt gttaataata ccttaatcca gggtagggag
     3961 acgttgcaca atgcattgca ttatatatac ctatcactga tttaatgttc caatgtagat
     4021 agacactatt aaataagcag taattccttg aaaggagtac ctgtaccaag tcctaggaaa
     4081 taatgcttgt aataaactta actgtgttac atcagtaggt aaaatacata atgttacata
     4141 gtgtaatatg atatgatgtg attaaattat agttcctgct gttaaattgc cttttgaaaa
     4201 cttggctcca gggttggtgc ttcttaaatg gggaaaggca cagtgaactt cagccatatt
     4261 aaggacgtgg tctgtggttt atcaccagac ttgttcactc cattcaatta tgtcctgagc
     4321 cttgaaactg ttttatttaa ccatggggta ggctggggtg atgtttcagt tttcaaggga
     4381 cagaagcaag acaaaatgca tgggcccttg tgttcctaat ggtcttaact gaagtccaac
     4441 ttcagttaca attttcagag gctcgttgtt ttcccaaagc taaatcttta tactaaatgc
     4501 aatactgact ttttcttaag tagctaaaga ccgagagtat actatcttta gggatagtca
     4561 tttctcaaat gagttctcta atagagattt tgttcctaca atgtagtctg cactgaaaag
     4621 agaatagagg agggtagggt ggtcattttg caggaatttt tagtcagatt ttgggggtag
     4681 ataccatccc tttttttatt aagaaaatca tatatttcca acatatttta aatgtctata
     4741 tcttttatta tagagaaacc attagatcca ataaatgaag aaatacattt taagtagata
     4801 tcagttactt ttaatttgta tggtaaatat atacataggt ccatttcaga aaaaaaatca
     4861 tgattgtaaa gggaatatat aaccacacag attttatagc tgcttcctgg ttatcactta
     4921 cattccgtaa ggagctgttc cttaggaccg gttggaagta gaagaaacat ctctccctgc
     4981 acccgaatgt ccagtggagc ctcaagtttt ccatcctgct gttaagttac tttcaaatag
     5041 tttaatgttt tagtcagaga aaaaaaaata tacaattata taatatctca tttaataagt
     5101 ttaatacata caaagataat ctatatattt taccaattta atgtgaattt taaaaaatcc
     5161 ctatgctatt taaaagaaag aaaatgtatt ttccatagta gttttgtgta aatcgtatga
     5221 ttcacctcat ctttaatatg cattgaaata ggtaatacta gatgcaagtg tttccaaagc
     5281 aaaaaaggtg caattttaaa gaatacatag ccaaagcaat gttactaccc atcatactgt
     5341 ttaaaaatgg atagaaaata aaaatataag ttagtatggt ttaattctga aaatacaaat
     5401 tatcttcctt taagaaacct accattaagc caggtgtgga ttgacacacc tttagtctca
     5461 ggactgagga ggcgaggcaa gcagatccct gagtttgagg taagcctgat ttacagagca
     5521 agttccaaga caccaggcaa tacagagaaa cctgcctcaa gccatctgct ccctgaaaaa
     5581 agagataaat ccttaataac caactataca aagactgtta aatgatacag aaaggaaaaa
     5641 gagaaaggaa ggaaaggagg gaggaaggga agaaaggaac tcatgctaca agatagagag
     5701 taggtaccca ggagtggaga tggaaggagt ctgaactttc ctgtggccat gctgacttac
     5761 actaagattc taccccagaa aacaattctt tagcagtgga cagaaccctt aaattctaaa
     5821 aagctacaaa catcaagcaa ttcaggtact aggataacat gtcagttttg ctgggttgga
     5881 ggtgggtatc aagtgattat ttcctatcaa gacatcttta aattatagag ctcagtaatc
     5941 aatttaaaga cagtgttaag ctactcacag tggcaaatgc cttttatcct agcactcctt
     6001 aagcagaggc aaacaggttt cttgagttca ggccagtcag gtctgcataa ggagctttag
     6061 accagtcaat gatacatagt gaaaccctac ctcaaaaaca aaacaaaaaa aatgttttta
     6121 cgcattttat caaaattagc acatctgtgg ctgaggagaa atccacctga aaatgtggga
     6181 gaccttgaca gagtggtcag ctggatcagt gtgccctaaa ctaagtaaaa tcattgttcc
     6241 attcttatac atttgaatgt gatggtgacc tttgaaacaa ttccaggggc attctcaagt
     6301 atttgatacc taggggttta gtaactcaga caaattaatc cctttgtact aattttatga
     6361 aacagagctg ggggtgggga cagctctggc atagggggcc tcattaacac tcttaactct
     6421 gggatagaac cctaagcaac aacaacaaca aaagtgtcat attttagaag gcctagaggt
     6481 gactcatttt agatatatat actttgtttt ggaatatttg gttgtataaa atgtaatgag
     6541 tgagctaatt gattttttag accatatttg aagatgtgga tgacttacaa gaaacacaaa
     6601 agaaaggtac ttatagagtc tggttctaat aattggtacc ctctttctcg gttccatatt
     6661 tttaacaacc ctttaacaaa ctaaataatt tttaagcagc caggaaattt tagaggacag
     6721 atatttgacc aaaagactat caactttaaa catttatttt atggtttctt gtcaagattt
     6781 aatctaaata ttttaaaagc aattccaagg aagcatacaa gattttcttt taaaataaca
     6841 aatttaatat aaatcaaaga ccagcatttt aactacaaat ggtagttgtt taaccatcat
     6901 atttctcttt aaagtacaca aaattcttgg atctggaaag ataactcaac agtcaagagt
     6961 actggttgtt cttccccaag gaccaaggtt caattcctag cacccatatg actgctcaca
     7021 ggcacctgta actccattcc cagaggaatc agcaccatct tttagcccca gcaggcacct
     7081 caggcaaaca ttcattcaca taaaataaac atacaatatc caaaaatata ttcttttcac
     7141 atattcagtg tctcctaaca aactagtact ggacttacag tactgccaaa tctttgttaa
     7201 gtttcatgat gtgttagtac actgttctat tgctgtaaag agacaccatg acaaagacaa
     7261 ctcatataaa aggaagtgtt gagttggggg cttgcttata gtttcagagt tgagtttatt
     7321 tgcatggtgt caggcaggca ggatggtgcc ggagaagtag ctgagagata catcctgatc
     7381 tacaggcgtg agagacacag agagagatag acactgggcc ttatatgctc ttttaaaact
     7441 tcaaggtcca cccccagtaa tgcacttcct tcaaaagacc ccacctccta atccttgtaa
     7501 tcttcctcaa accagttctg ggctctagtg actagacatt caaatatatg agcctatggg
     7561 aaccattctc attcaaacca ctaaacatag ccaaattctt agttatttta gacagtaaag
     7621 gggcttctag aatgttaaga atgttgaaaa tcaaactgct tcaaaggaaa gtaaaaaaaa
     7681 aaaaaaaaaa aaaaaagaaa aactgtggaa gtagccagcc ttgtacgact gcaaaagcag
     7741 cctcgctgac tggatttttt ttttttttaa agatttattt atatgagtac actgtggctg
     7801 tctcagacac accagaagag ggcatcagat cccattacag atggttgtga gccaccatgt
     7861 ggttgcttta agatttatgt atatgagtac actgtggctg tctcagacac accagaagag
     7921 ggcatcagat cccattacag atggttgtga gccaccatgt ggttgctggg aattgaactc
     7981 aggacctttg gataagaaac cagtgttctt aactgctgag ccatctctcc agttagctaa
     8041 ctggattctt aaggatgatt ctagcaaatt ataagaataa atagtaaaaa gaggcaggga
     8101 catatttgtt aacaatatat tgaaatggga gctttcaagt caaggagcca aacatagtaa
     8161 ctaaatcatg accatctgat gcaagatgca gtcatatcca tggtactgaa aataacatga
     8221 tgcttgcttg gagtatgatg ctatgtcacc caagtcacag gatgattatt aaaagaacac
     8281 acacaccaaa aaacatactt atcctaaaga gatggtgaga tataacatgc cttaaattat
     8341 attatagaat ttaaataact agctttcaca atcagtttgt gtgtaaaatg tcatttaaag
     8401 tgttttcaaa tgcattgctc ttacaattta ttttccttta actcttctat gatatttaaa
     8461 gcgtgcatct gccacattga tgattaaatt agcagtttag ataacttgag tccctttccc
     8521 tttatatttg agatgaggtc tagatatgta gtcctgcctg gcctccaatt tgtaatcctc
     8581 ctgcctcagc cttgagtgct ggaattgagt gtgtgccacc ttgaccagcc ttttgtttcc
     8641 tttttaatac taaaccttac agtcaggatt tggagttctt acatcatata taatatgtaa
     8701 taaaccagga tgaatattgc atattcaata tctccagttt taatagtggc attgaagtgg
     8761 ttctggacca aaagaagtca gtttgtgaat gttgtctgtt aagtattaca aaataacaag
     8821 gaaaaaaaaa caaaataaca agggttagaa gttcaaagta aaaaacagac atctttgatt
     8881 tctgattaag aaaaagcact ctacatcttt tttttttaag ttttctaagt ttgttccttc
     8941 tgagagatta agtaaaaaac acctttaaaa tcatgtcagc ctgcttagaa tgtacatgtt
     9001 aaaaagcaat ttaagatgaa aaaactagct ttacagtgtg tacacttttt ctctaaaata
     9061 aacttttgtg gctttagtgt cttagaagga gagtcctaac atgaaatggt catctcttag
     9121 atttgggagg aaggaagcca ctgttaactg tgtagtgtta cagcatattg cacaaaaggt
     9181 tttggttttg gtctggtgta gctttggctg tcctgaaatg aaacactcac tatataaacc
     9241 acacttgcct cacagagatc ctcctgcctc tgtctcctga atgttgggac aaaagtatgt
     9301 gccaccccta cccagcgaga acagttttta atgaaaactg agctagattg tccagttctg
     9361 gtaaacagtc ttccagtcat ctcacaacca ttattacaac catgtacaaa gggactccac
     9421 tccactgtcc ctgtacaagg acttttgaca gtttagtgct tagtaactag tcttagtttt
     9481 cttcctttta gaaaaactgt acatttgatc tctcaattct gtttgcaaag cagtgcagaa
     9541 aactataatc aaatgtgaca tgtctataaa ataattttga cataacagac atgaacagac
     9601 ataagaacgg cttccaatgt taattgataa tctataaata tcattttgga atttgactgg
     9661 ttcacaaata tatccacaaa atgtttgcat atggaataat agaagcatat aaaacattgt
     9721 atgtaaattg catcttcata ctagcaaaca agttaacaaa tgataataaa tgcaattcag
     9781 ttttggatgt tggtccacat tgtcaagaag tcatgtaata ataataaata catattctac
     9841 ctgctacttg aa
//
