LOCUS       NM_028306               2945 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus heat shock protein 12B (Hspa12b), mRNA.
ACCESSION   NM_028306
VERSION     NM_028306.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Fan,M., Yang,K., Wang,X., Zhang,X., Xu,J., Tu,F., Gill,P.S., Ha,T.,
            Williams,D.L. and Li,C.
  TITLE     LACTATE IMPAIRS VASCULAR PERMEABILITY BY INHIBITING HSPA12B
            EXPRESSION VIA GPR81-DEPENDENT SIGNALING IN SEPSIS
  JOURNAL   Shock 58 (4), 304-312 (2022)
   PUBMED   36256626
  REMARK    GeneRIF: LACTATE IMPAIRS VASCULAR PERMEABILITY BY INHIBITING
            HSPA12B EXPRESSION VIA GPR81-DEPENDENT SIGNALING IN SEPSIS.
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Fan,M., Yang,K., Wang,X., Wang,Y., Tu,F., Ha,T., Liu,L.,
            Williams,D.L. and Li,C.
  TITLE     Endothelial cell HSPA12B and yes-associated protein cooperatively
            regulate angiogenesis following myocardial infarction
  JOURNAL   JCI Insight 5 (18), 139640 (2020)
   PUBMED   32790647
  REMARK    GeneRIF: Endothelial cell HSPA12B and yes-associated protein
            cooperatively regulate angiogenesis following myocardial
            infarction.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2945)
  AUTHORS   Tu,F., Wang,X., Zhang,X., Ha,T., Wang,Y., Fan,M., Yang,K.,
            Gill,P.S., Ozment,T.R., Dai,Y., Liu,L., Williams,D.L. and Li,C.
  TITLE     Novel Role of Endothelial Derived Exosomal HSPA12B in Regulating
            Macrophage Inflammatory Responses in Polymicrobial Sepsis
  JOURNAL   Front Immunol 11, 825 (2020)
   PUBMED   32457753
  REMARK    GeneRIF: Novel Role of Endothelial Derived Exosomal HSPA12B in
            Regulating Macrophage Inflammatory Responses in Polymicrobial
            Sepsis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2945)
  AUTHORS   Zhang,X., Wang,X., Fan,M., Tu,F., Yang,K., Ha,T., Liu,L.,
            Kalbfleisch,J., Williams,D. and Li,C.
  TITLE     Endothelial HSPA12B Exerts Protection Against Sepsis-Induced Severe
            Cardiomyopathy via Suppression of Adhesion Molecule Expression by
            miR-126
  JOURNAL   Front Immunol 11, 566 (2020)
   PUBMED   32411123
  REMARK    GeneRIF: Endothelial HSPA12B Exerts Protection Against
            Sepsis-Induced Severe Cardiomyopathy via Suppression of Adhesion
            Molecule Expression by miR-126.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2945)
  AUTHORS   Zhao,Y., Liu,C., Liu,J., Kong,Q., Mao,Y., Cheng,H., Li,N.,
            Zhang,X., Li,C., Li,Y., Liu,L. and Ding,Z.
  TITLE     HSPA12B promotes functional recovery after ischaemic stroke through
            an eNOS-dependent mechanism
  JOURNAL   J Cell Mol Med 22 (4), 2252-2262 (2018)
   PUBMED   29411514
  REMARK    GeneRIF: HSPA12B promoted functional recovery and survival after
            stroke in an eNOS-dependent mechanism.
REFERENCE   6  (bases 1 to 2945)
  AUTHORS   Zhang,R., Wan,X.J., Zhang,X., Kang,Q.X., Bian,J.J., Yu,G.F.,
            Wang,J.F. and Zhu,K.M.
  TITLE     Plasma HSPA12B is a potential predictor for poor outcome in severe
            sepsis
  JOURNAL   PLoS One 9 (6), e101215 (2014)
   PUBMED   24977412
  REMARK    GeneRIF: Plasma extracellular HSPA12B is elevated in both septic
            mice and patients.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2945)
  AUTHORS   Li,J., Zhang,Y., Li,C., Xie,J., Liu,Y., Zhu,W., Zhang,X., Jiang,S.,
            Liu,L. and Ding,Z.
  TITLE     HSPA12B attenuates cardiac dysfunction and remodelling after
            myocardial infarction through an eNOS-dependent mechanism
  JOURNAL   Cardiovasc Res 99 (4), 674-684 (2013)
   PUBMED   23729663
  REMARK    GeneRIF: inhibition of eNOS abrogated the protection of HSPA12B
            against cardiac dysfunction and remodelling after myocardial
            infarction
REFERENCE   8  (bases 1 to 2945)
  AUTHORS   Coulpier,F., Le Crom,S., Maro,G.S., Manent,J., Giovannini,M.,
            Maciorowski,Z., Fischer,A., Gessler,M., Charnay,P. and Topilko,P.
  TITLE     Novel features of boundary cap cells revealed by the analysis of
            newly identified molecular markers
  JOURNAL   Glia 57 (13), 1450-1457 (2009)
   PUBMED   19243017
REFERENCE   9  (bases 1 to 2945)
  AUTHORS   Steagall,R.J., Rusinol,A.E., Truong,Q.A. and Han,Z.
  TITLE     HSPA12B is predominantly expressed in endothelial cells and
            required for angiogenesis
  JOURNAL   Arterioscler Thromb Vasc Biol 26 (9), 2012-2018 (2006)
   PUBMED   16825593
REFERENCE   10 (bases 1 to 2945)
  AUTHORS   Han,Z., Truong,Q.A., Park,S. and Breslow,J.L.
  TITLE     Two Hsp70 family members expressed in atherosclerotic lesions
  JOURNAL   Proc Natl Acad Sci U S A 100 (3), 1256-1261 (2003)
   PUBMED   12552099
  REMARK    GeneRIF: HSPA12A and HSPA12B may play roles in genetic
            susceptibility to atherosclerosis
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AL831736.16.
            
            On Nov 17, 2006 this sequence version replaced NM_028306.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC011103.1, AK012548.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               AL831736.16        59034-59136
            104-163             AL831736.16        65685-65744
            164-261             AL831736.16        67417-67514
            262-386             AL831736.16        68562-68686
            387-573             AL831736.16        70051-70237
            574-678             AL831736.16        70603-70707
            679-795             AL831736.16        71027-71143
            796-970             AL831736.16        72521-72695
            971-1057            AL831736.16        73454-73540
            1058-1162           AL831736.16        74272-74376
            1163-1421           AL831736.16        74475-74733
            1422-1525           AL831736.16        75244-75347
            1526-2945           AL831736.16        76188-77607
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 63.27 cM"
     gene            1..2945
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /note="heat shock protein 12B"
                     /db_xref="GeneID:72630"
                     /db_xref="MGI:MGI:1919880"
     exon            1..103
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            104..163
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             121..2178
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /codon_start=1
                     /product="heat shock 70 kDa protein 12B"
                     /protein_id="NP_082582.1"
                     /db_xref="CCDS:CCDS16755.1"
                     /db_xref="GeneID:72630"
                     /db_xref="MGI:MGI:1919880"
                     /translation="MLTVPEMGLQGLYISSSPERSPVPSPPGSPRTQESCGIAPLTPS
                     QSPKPEARALQQASFSVVVAIDFGTTSSGYAFSFASDPEAIHMMRKWEGGDPGVAHQK
                     TPTCLLLTPEGIFHSFGYTARDYYHDLDPEEARDWLYFEKFKMKIHSATDLTLKTQLE
                     AVNGKKMLALEVFAHALRFFKEHALQELREQSECMLEKGAVRWVLTVPAIWKQPAKQF
                     MREAAYLAGLVSREDAEKLLIALEPEAASVYCRKLRLHQLMDLSSRTAGRGRLGERRS
                     IDSSFRHAREQLRRSRHSRTFLVEAGVGELWAEMQEGDRYMVADCGGGTVDLTVHQLE
                     QPHGTLKELYKASGGPYGAVGVDLAFEQLLCRIFGEDFIAKFKRQRPAAWVDLTIAFE
                     ARKRTAGPHRAGALNISLPFSFIDFYRKQRGHNVETALRRSSVNLVKWSSQGMLRMSC
                     EAMNELFQPTVSGIIQHIEMLLAKPEVQGVKLLFLVGGFAESAVLQHAVQEALGTRGL
                     RVVVPHDVGLTILKGAVLFGQAPGVVRVRRSPLTYGVGVLNRFVPGHHPPEKLLVRDG
                     RRWCTDVFERFVAAEQSVALGEEVRRSYCPARPGQRRVLINLYCCAAEDARFITDPGV
                     RKCGALSLELEPEGCPENTGTSPSRREIRAAMQFGDTEIKVTAVDVSTNRSVRAAIDF
                     LSN"
     exon            164..261
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            262..386
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            387..573
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            574..678
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            679..795
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            796..970
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            971..1057
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1058..1162
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1421
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1525
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..2945
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2293..2298
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      2314
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
     regulatory      2921..2926
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2945
                     /gene="Hspa12b"
                     /gene_synonym="2700081N06Rik"
                     /note="major polyA site"
ORIGIN      
        1 gagacgggcc tggtgcacgt tgaggtccac tgtaagctcc gctgcgggac tctgagctag
       61 cacccaggcg acaagacccg gaggagctgt ccgaggggcg gagctacaga gactgcaagg
      121 atgctgactg tcccggaaat gggcctgcaa gggctgtata tcagctctag cccagagagg
      181 tccccggtgc ccagccctcc cggctccccg aggacccagg aaagctgtgg tatcgctccc
      241 ctcacgcctt cacagtctcc aaagccagag gcccgagctc tacagcaggc ttccttctct
      301 gtggttgtgg ccatcgactt tggaaccaca tccagtggct atgccttcag ctttgctagt
      361 gaccctgagg ccattcacat gatgaggaaa tgggagggag gggacccagg cgtggcccac
      421 cagaagaccc ccacttgcct gctgctgacc ccagaaggca tctttcacag ttttggctac
      481 actgcccgtg actactacca cgacctagac cctgaggagg ctcgggactg gctctacttt
      541 gagaagttta agatgaagat tcacagtgcc actgatctca ccttgaagac ccagctcgag
      601 gcggtaaatg ggaagaagat gctggctctg gaagtgtttg cccatgccct ccgtttcttc
      661 aaggagcacg cccttcagga gctgagagag cagagcgagt gtatgcttga gaagggtgct
      721 gtgcgctggg tgctgacagt acctgccatc tggaaacaac cggctaaaca gttcatgaga
      781 gaggccgcct acctggctgg cttggtgtct cgagaggatg cagagaaact cctcattgcc
      841 ctggagcctg aggctgcctc tgtctactgt cggaaacttc gcctgcacca gctcatggac
      901 ctgagtagcc ggactgcagg ccgtgggcgc ctgggtgagc ggaggtctat cgactccagc
      961 tttcgacatg cccgggagca gctgcgtagg tctcgccaca gccgaacttt cctggtggag
     1021 gccggtgtgg gagagctgtg ggcagagatg caagaaggag accgctacat ggtggccgac
     1081 tgcgggggtg gcactgtgga cctgacggta caccagttgg agcagcctca cgggaccctc
     1141 aaggagctct acaaggcttc tggcggtccc tatggagcag taggcgtgga cttggccttt
     1201 gagcagctgc tctgccgcat attcggggag gacttcattg ccaaattcaa aaggcaacgg
     1261 ccagcagctt gggtggatct aaccattgcc ttcgaggccc gcaaacgcac ggcaggcccg
     1321 caccgtgcgg gggcgctcaa catttcgctt cctttctctt tcattgactt ctaccgcaag
     1381 caacgagggc acaatgtgga gaccgccctg cgcaggagca gtgtgaacct cgtgaagtgg
     1441 tcctcacagg ggatgctccg gatgtcctgt gaggctatga atgagctgtt tcaacccacg
     1501 gtcagtggga tcatccaaca catagagatg ctgctggcta agcctgaggt gcagggtgtg
     1561 aagttgctat tcctggtggg cggctttgcg gagtcggcgg tgctgcagca cgcggtgcag
     1621 gaagccctgg gcactcgtgg cctgcgcgtt gtggtcccgc atgacgtggg cctcaccatc
     1681 ctcaagggag cagtgctctt cggccaggca cccggtgtgg tgcgagtgcg ccgctccccc
     1741 ctcacttatg gtgtgggcgt gctcaatcgt tttgtgcctg gccaccaccc acccgagaaa
     1801 ttgctggttc gggatgggcg ccgctggtgc acggatgtct ttgagcgctt cgtagcagca
     1861 gagcagtcgg tggccctagg agaggaggta aggcgcagct actgtcctgc gcgcccgggc
     1921 cagcggcgtg tgctcatcaa tctgtactgc tgcgctgcgg aggatgcgcg cttcatcact
     1981 gaccctggtg tgcgcaagtg tggtgcactc agcctggagc ttgagcctga aggctgcccg
     2041 gaaaacacag gcacatcccc cagccgccgt gagatccgtg ctgccatgca gtttggcgat
     2101 actgagatta aggtcactgc cgtggatgtc agcaccaatc gctctgtgcg ggcagccatc
     2161 gactttcttt ccaactgagg gtgcaccctt cggtggggac acaagaacgt gctagttttc
     2221 acagcagcac ccctttctcg gggtaaggac acatggagac ccgctttagg gctgtgcctt
     2281 gatcactgga acactaaaag tccccttacc ccaagaggcg aggtggtgta atagctaaga
     2341 agtttgctta gagggtgagc ctggcttggc gagtgtgtga ccacgaagat agactgcaac
     2401 agaggactaa acactgacct gaacttgagg ttaggtgaag ctgctgagga gccacggggg
     2461 tgggggtggg attgggaggg ggtgcagaac cgggactgaa ggttagagtt ggctaacttt
     2521 gagttcaagc cacaggaagc ccaagagagt aatctcatgc agggggagta gggaagggag
     2581 tgttaggccc atgctagagg gacaggtgtg agtagcacct tcctggaggt gtgactgtta
     2641 attgtggctg cactgccaca gtgaaagcag tttggagatg tggacacagc atgctcaaag
     2701 gacctacagg aaagctataa atgactcatt tgcccccaac ccagtcacag gaggacagag
     2761 ttactgtggc aagggggcgg gggtttccag ctctgccaca tatcagtata ccattaggaa
     2821 atctgaccaa tcagtttctc caagtgtgaa atgtcagaca tttcctgcca accatggcgt
     2881 aagacagctt tccctgcctg agaacttatc ctaaggtgaa aataaagttt gcaaggtaag
     2941 gaaaa
//
