LOCUS       NM_024255               2632 bp    mRNA    linear   ROD 01-OCT-2024
DEFINITION  Mus musculus hydroxysteroid dehydrogenase like 2 (Hsdl2),
            transcript variant 1, mRNA.
ACCESSION   NM_024255
VERSION     NM_024255.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Samson,N., Bosoi,C.R., Roy,C., Turcotte,L., Tribouillard,L.,
            Mouchiroud,M., Berthiaume,L., Trottier,J., Silva,H.C.G.,
            Guerbette,T., Plata-Gomez,A.B., Besse-Patin,A., Montoni,A.,
            Ilacqua,N., Lamothe,J., Citron,Y.R., Gelinas,Y., Gobeil,S.,
            Zoncu,R., Caron,A., Morissette,M., Pellegrini,L., Rochette,P.J.,
            Estall,J.L., Efeyan,A., Shum,M., Audet-Walsh,E., Barbier,O.,
            Marette,A. and Laplante,M.
  TITLE     HSDL2 links nutritional cues to bile acid and cholesterol
            homeostasis
  JOURNAL   Sci Adv 10 (22), eadk9681 (2024)
   PUBMED   38820148
  REMARK    GeneRIF: HSDL2 links nutritional cues to bile acid and cholesterol
            homeostasis.
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2632)
  AUTHORS   Steinmetz,C.C., Tatavarty,V., Sugino,K., Shima,Y., Joseph,A.,
            Lin,H., Rutlin,M., Lambo,M., Hempel,C.M., Okaty,B.W., Paradis,S.,
            Nelson,S.B. and Turrigiano,G.G.
  TITLE     Upregulation of mu3A Drives Homeostatic Plasticity by Rerouting
            AMPAR into the Recycling Endosomal Pathway
  JOURNAL   Cell Rep 16 (10), 2711-2722 (2016)
   PUBMED   27568566
REFERENCE   4  (bases 1 to 2632)
  AUTHORS   Calvo,S.E., Pagliarini,D.J. and Mootha,V.K.
  TITLE     Upstream open reading frames cause widespread reduction of protein
            expression and are polymorphic among humans
  JOURNAL   Proc Natl Acad Sci U S A 106 (18), 7507-7512 (2009)
   PUBMED   19372376
  REMARK    GeneRIF: Includes the study of an upstream ORF in this gene, and
            shows that it functions to reduce protein levels by ~35%.
REFERENCE   5  (bases 1 to 2632)
  AUTHORS   Dai,J., Li,P., Ji,Ch., Feng,C., Gui,M., Sun,Y., Zhang,J., Zhu,J.,
            Dou,Ch. and Gu,Sh.
  TITLE     [Cloning and characterization of a novel mouse short-chain
            dehydrogenase/reductases cDNA mHsdl2#, encoding a protein with a
            SDR domaid and a SCP2 domain]
  JOURNAL   Mol Biol (Mosk) 39 (5), 799-805 (2005)
   PUBMED   16240713
  REMARK    GeneRIF: Hsdl2 is widely expressed in normal mouse tissues and its
            expression level in liver increases after inducement with
            cholesterol food.
REFERENCE   6  (bases 1 to 2632)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   7  (bases 1 to 2632)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY327952.1, AY725196.1, AA545425.1 and BE944922.1.
            
            On May 5, 2010 this sequence version replaced NM_024255.2.
            
            Transcript Variant: This variant (1) represents the shorter
            transcript but encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136773.1, AK085899.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            regulatory uORF        :: PMID: 19372376
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-143               BY327952.1         2-144
            144-1989            AY725196.1         1-1846
            1990-2372           AA545425.1         42-424
            2373-2632           BE944922.1         1-260               c
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 32.69 cM"
     gene            1..2632
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /note="hydroxysteroid dehydrogenase like 2"
                     /db_xref="GeneID:72479"
                     /db_xref="MGI:MGI:1919729"
     exon            1..213
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      119..184
                     /regulatory_class="uORF"
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /experiment="DESCRIPTION:regulatory uORF[PMID:19372376]"
                     /note="this uORF is predicted to encode a 21 aa peptide
                     (PMID:19372376)"
     CDS             197..1669
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hydroxysteroid dehydrogenase-like protein 2"
                     /codon_start=1
                     /product="hydroxysteroid dehydrogenase-like protein 2
                     isoform 1"
                     /protein_id="NP_077217.2"
                     /db_xref="CCDS:CCDS38772.1"
                     /db_xref="GeneID:72479"
                     /db_xref="MGI:MGI:1919729"
                     /translation="MLPNTGKLAGCTVFITGASRGIGKAIALKAAKDGANIVIAAKTT
                     QKHPKLLGTIYTAAEEIEAAGGTALPCVVDVRDEQQINSAVEKAVEKFGGIDILVNNA
                     SAISLTNTLDTPTKRVDLMMNVNTRGTYLTSKACIPFLKKSKVGHILNLSPPLNLNPL
                     WFKQHCAYTIAKYGMSMCVLGMAEEFRGEIAVNALWPRTAIHTAAMDMLGGSGVENQC
                     RKVDIIADAAYSIFKRPKSFTGNFIIDENILKEEGIKNFDVYAIAPGHPLLPDFFLDE
                     HPDAVMEEKESNDSVPEVKEEKLQLQEESQLQKQPQLQEQPQLQEKPQLQEKPQLQEQ
                     PQLQEKPQLQEQPQQREQPQLQQQPRPRQQPQPFVQSMLPQKPHFGAVEETFRIVKDS
                     LSDEVVRATQAVYQFELSGEDGGTWFLDLKSKGGKVGHGEPSDRADVVMSMATDDFVK
                     MFSGKLKPTMAFMSGKLKIKGNIALAIKLEKLMTQMNSRL"
     exon            214..377
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            378..476
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            477..591
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            592..695
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            696..794
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            795..989
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1061
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1062..1427
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1556
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..2616
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1967..1972
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1989
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /note="major polyA site"
     regulatory      2585..2590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2611
                     /gene="Hsdl2"
                     /gene_synonym="2610207I16Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtgcccggt gtgcgcaggc gcacaccgtc ttgtaagctc tcggtcttgt aggatctcgg
       61 tcttgtagga gggccggtcc ccgagcgggt ctcggggcgg ggcccggggc gcggctaaat
      121 gcggagagaa agttcgcctg tcactcacag ctcgctgctg ttccactgcc accaagttct
      181 ctgaactgcg aaggtcatgc tgcccaacac tgggaagcta gcgggatgca cagtttttat
      241 cacaggtgca agccgaggca ttggcaaagc aattgccttg aaagccgcaa aggatggagc
      301 caatattgtc attgctgcga agaccaccca aaagcacccg aaactcctcg gcacaatcta
      361 cacggctgct gaagaaattg aagcagctgg agggacggcc ttgccttgtg ttgttgatgt
      421 gagagatgaa cagcaaatca acagtgcagt ggagaaagct gtggagaaat ttggaggaat
      481 tgatattttg gtgaacaatg ccagtgctat tagcttgacc aacacgttgg acactccgac
      541 caagagagtg gacttgatga tgaatgtgaa caccaggggc acctacctta catccaaagc
      601 atgtattcct tttttaaaaa agagcaaagt aggtcatatt ctcaatctca gcccacccct
      661 gaacctaaac ccactgtggt tcaaacagca ctgtgcttat accattgcca aatatggcat
      721 gtctatgtgt gtgcttggga tggctgaaga atttagaggt gaaattgcag tcaatgcctt
      781 atggcctaga acagccattc acactgctgc tatggatatg ctgggaggat ctggtgttga
      841 aaaccaatgt agaaaagttg acatcattgc ggacgctgca tattccattt tcaaaaggcc
      901 aaaaagtttt actggcaact ttattattga tgaaaatatc ttaaaagaag aaggaataaa
      961 aaattttgat gtctacgcaa ttgcaccagg tcatcccttg ttaccggatt tcttcttaga
     1021 tgaacaccca gatgcagtta tggaggagaa agaatcaaat gactctgtgc cagaagtgaa
     1081 agaagagaag ctgcagctgc aggaggagtc acagctacag aagcagccac agctgcagga
     1141 gcagccacag ctgcaggaga agccacagct gcaggagaag ccacagctgc aggagcagcc
     1201 acagctgcag gagaagccac agctgcagga gcagccacag caacgggaac agccacagct
     1261 gcagcagcag ccacggccac ggcaacagcc acagccattt gtgcagtcaa tgctaccaca
     1321 aaaaccacat tttggagctg tggaagaaac atttagaatt gttaaggact ctctcagtga
     1381 tgaagttgtt agagcgacac aagctgtcta tcagtttgaa ctctcaggtg aagatggtgg
     1441 tacgtggttt cttgatctaa agagcaaagg cgggaaggtt ggacacggag agccctctga
     1501 ccgggcagac gtggtgatga gtatggccac tgatgatttt gtcaaaatgt tttcagggaa
     1561 attaaagcca accatggcat ttatgtcagg aaagttgaag attaaaggaa acatagccct
     1621 agcaatcaaa ttggagaagc taatgaccca gatgaattct agactatgaa ggacaagaag
     1681 taacaatgtt gactctcctc agaagtaaaa agctcagcag ttagaaatct gttgtctccc
     1741 ccactgtcat gtaaggacat ataagtttgt tctagaaaga agtagaattt ctgtatctat
     1801 aagatttgaa actgtaacta aaacaactaa ccaatcacat acaagtttaa ttaagtgaca
     1861 tcctaatata ttgtggtttt tccaagtttt cctctctatg cctttatttc attcattgct
     1921 ttcttgcaga aaagtatgta tcatgaacaa ccaattggta tcaagcaata aaattagctg
     1981 tttacaaaac ttcttatgta aaatgaagct actttatctc cacaaagaca gttgcatgag
     2041 ggttgggtga agaactgcag tatcatttct ttctgtaacc ctgactggct tgggactcac
     2101 cctgtagact aggctgggca cgaactcaca gaggtctgcc tgcccctgcc tcctgagtgc
     2161 tttgactgaa ggtgtgctcc accaccacct ggcggttttt ttttttttaa ggacttttat
     2221 tttgcctggc tgtgttttat gaacttcagt gcataatact tgcaggcgaa atcttgttta
     2281 acttattata agcatttata ctattgatac tattctagat atcttaaaat tttcagcttt
     2341 tctttttttt ccaaatgggt taacccaact ggccttaaac tggggtcagt cctcctgcct
     2401 cagttttctt agtgctatta ttatagacat gagacacaat gcctggcttt attgagcttt
     2461 caagtactaa catatagaaa tataattaat tttgtgagta tgccttatgc cctacagtag
     2521 tcagatctac tcattacctt tagcagcttc ttaaaaacag attctttaaa tcgtgttatc
     2581 ttcaaataaa gatgaattta tcttttttcc attctaaaaa aaaaaaaaaa aa
//
