LOCUS       NM_133943               1773 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and
            steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, mRNA.
ACCESSION   NM_133943
VERSION     NM_133943.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Li,J., Lu,E., Yi,T. and Cyster,J.G.
  TITLE     EBI2 augments Tfh cell fate by promoting interaction with
            IL-2-quenching dendritic cells
  JOURNAL   Nature 533 (7601), 110-114 (2016)
   PUBMED   27147029
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Yi,T. and Cyster,J.G.
  TITLE     EBI2-mediated bridging channel positioning supports splenic
            dendritic cell homeostasis and particulate antigen capture
  JOURNAL   Elife 2, e00757 (2013)
   PUBMED   23682316
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1773)
  AUTHORS   Yi,T., Wang,X., Kelly,L.M., An,J., Xu,Y., Sailer,A.W.,
            Gustafsson,J.A., Russell,D.W. and Cyster,J.G.
  TITLE     Oxysterol gradient generation by lymphoid stromal cells guides
            activated B cell movement during humoral responses
  JOURNAL   Immunity 37 (3), 535-548 (2012)
   PUBMED   22999953
  REMARK    GeneRIF: Findings indicate that CYP7B1 and HSD3B7, as well as
            CH25H, have essential roles in controlling oxysterol production in
            lymphoid tissues.
REFERENCE   4  (bases 1 to 1773)
  AUTHORS   Shea,H.C., Head,D.D., Setchell,K.D. and Russell,D.W.
  TITLE     Analysis of HSD3B7 knockout mice reveals that a 3alpha-hydroxyl
            stereochemistry is required for bile acid function
  JOURNAL   Proc Natl Acad Sci U S A 104 (28), 11526-11533 (2007)
   PUBMED   17601774
  REMARK    GeneRIF: Catalyzes two reactions required for inversion of the
            cholesterol 3beta-hydroxyl group to the 3alpha-hydroxyl of bile
            acids; its stereochemical alteration eliminates cholesterol
            absorption in the gut and enterohepatic circulation feedback
            regulation.
REFERENCE   5  (bases 1 to 1773)
  AUTHORS   Schwarz,M., Wright,A.C., Davis,D.L., Nazer,H., Bjorkhem,I. and
            Russell,D.W.
  TITLE     The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid
            oxidoreductase is mutated in progressive intrahepatic cholestasis
  JOURNAL   J Clin Invest 106 (9), 1175-1184 (2000)
   PUBMED   11067870
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC149222.4.
            
            On Dec 21, 2021 this sequence version replaced NM_133943.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF277718.1, BC138589.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                AC149222.4         153348-153404       c
            58-229              AC149222.4         152816-152987       c
            230-385             AC149222.4         152446-152601       c
            386-494             AC149222.4         152120-152228       c
            495-594             AC149222.4         151949-152048       c
            595-757             AC149222.4         151664-151826       c
            758-1773            AC149222.4         150261-151276       c
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 69.74 cM"
     gene            1..1773
                     /gene="Hsd3b7"
                     /note="hydroxy-delta-5-steroid dehydrogenase, 3 beta- and
                     steroid delta-isomerase 7"
                     /db_xref="GeneID:101502"
                     /db_xref="MGI:MGI:2141879"
     exon            1..57
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     exon            58..229
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     CDS             64..1173
                     /gene="Hsd3b7"
                     /EC_number="1.1.1.181"
                     /note="isoform a is encoded by transcript variant 1; 3
                     beta-hydroxysteroid dehydrogenase type 7; 3-beta-HSD VII;
                     c(27) 3-beta-HSD; 3 beta-hydroxysteroid dehydrogenase type
                     VII; 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase;
                     cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase"
                     /codon_start=1
                     /product="3 beta-hydroxysteroid dehydrogenase type 7
                     isoform a"
                     /protein_id="NP_598704.2"
                     /db_xref="CCDS:CCDS40145.1"
                     /db_xref="GeneID:101502"
                     /db_xref="MGI:MGI:2141879"
                     /translation="MADSAQVPTLVYLVTGGCGFLGEHIVRMLLEREPRLRELRVFDL
                     HLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIH
                     KVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYP
                     CSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFR
                     AVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFL
                     SPCGLRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVS
                     TNKAQRHFGYKPLFSWEESRTRTIQWVQAMEGSAR"
     exon            230..385
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     exon            386..494
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     exon            495..594
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     exon            595..757
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     exon            758..1773
                     /gene="Hsd3b7"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1755..1760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hsd3b7"
                     /note="hexamer: AATAAA"
     polyA_site      1773
                     /gene="Hsd3b7"
                     /note="major polyA site"
ORIGIN      
        1 agtccaggac acaacctcct ccagtggcca gtttgtgcgc cctgggactt actacagctg
       61 ggcatggcag actctgcaca ggtgccaaca ctggtatacc tggtcacagg tggctgcggc
      121 ttcctggggg aacatattgt tcggatgctg ctggaacggg agcccaggct ccgggagctg
      181 cgtgtctttg acctgcacct gagttcttgg ctggaggagc tgaaagcagg gcctgtgcag
      241 gtgactgcca tccaggggga tgtgactcag gcccatgagg tggcagcagc catgtctgga
      301 tcacatgtgg tcatccatac agctgggttg gtggatgtgt ttgggaaggc cagtccaaag
      361 accatccaca aagtcaacgt gcagggcaca cagaatgtga ttgatgcttg tgtgcagact
      421 ggcactcagt acctggtcta cacgagcagc atggaagtgg tggggcctaa catcaagggc
      481 caccccttct acaggggcaa tgaagatacc ccatatgagg cagtccacag ccatccctac
      541 ccatgcagta aagcccttgc tgagcagctg gtcctcgagg ccaatggaag gaaggtcaat
      601 ggagggctac ccctggtgac atgtgccctt cgacccacgg gcatttatgg tgaaggtcat
      661 caggtcatga gagacttcta ctaccaggga ctgcgctttg gaggtcgtct atttcgggcc
      721 gtcccagctt ctgtggagca cggtcgggtc tatgttggca atgttgcttg gatgcacata
      781 ctggtggccc gggagctgga gcagcgggca gcactcatgg gtggccaggt gtatttctgc
      841 tatgataagt caccttataa aagctacgag gacttcaaca tggagtttct gagtccctgt
      901 ggtcttcgac tgataggcgc ccacccactg ctgccctact ggctgctagt gctgctggct
      961 accctcaatg ccctgctgca gtggctgctc cgcccactgg tgctgtacac acccctgctg
     1021 aatccctaca cgctggctat ggccaacacc acctttactg tcagtaccaa caaggcacag
     1081 cggcattttg gctacaagcc cctcttctca tgggaagaga gcaggacccg caccattcag
     1141 tgggtgcagg cgatggaggg ttcagctcgg tgatggcagg gccaggaact ggagaccact
     1201 gtggcacata ctccaggtcc tgagccccca aatctggatg aagagaaatg gctgcctttt
     1261 gaagatgaag actctgctct acacatcatg actccgtgat gtggaccctg ccacatctta
     1321 actacacaga tcctgagact ggttcatatt ccagtctcct agttctagga tagggtttgg
     1381 ggaaaggcct atctttggtg ccaactcagg cctgccaggt agttggcaaa actgatttct
     1441 tgaatggtct cactacctct tcctgtttcc agtttctcaa gcagggagga acagagactc
     1501 cagagatctt acgctggtct gcttacttca gctaccattt tcagacctgg ctcctgccac
     1561 atctggctct ccttaagggg ttatagtttc accatttttt aagtgtatct cttttagaca
     1621 tttatctttt aatttgcttt aaagaaatct gaagaaatca atgatttccc atgtcttgcc
     1681 tctcctaaca aattcaccta tgacattgtt agcttcctcc cctaggatgc caacctgtat
     1741 ctggccaagc ctagaataaa atccttttcc aag
//
