LOCUS       NM_001077202            4792 bp    mRNA    linear   ROD 29-AUG-2023
DEFINITION  Mus musculus heparan sulfate 6-O-sulfotransferase 2 (Hs6st2),
            transcript variant 1, mRNA.
ACCESSION   NM_001077202
VERSION     NM_001077202.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Tao C, Makrides N, Chuang JZ, Wu Y, Brooks SE, Esko JD, Sung CH and
            Zhang X.
  TITLE     Chondroitin sulfate enhances the barrier function of basement
            membrane assembled by heparan sulfate
  JOURNAL   Development 149 (12) (2022)
   PUBMED   35608020
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Patel VN, Pineda DL, Berenstein E, Hauser BR, Choi S, Prochazkova
            M, Zheng C, Goldsmith CM, van Kuppevelt TH, Kulkarni A, Song Y,
            Linhardt RJ, Chibly AM and Hoffman MP.
  TITLE     Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to
            reduce epithelial morphogenesis and adult salivary gland function
  JOURNAL   Matrix Biol 103-104, 37-57 (2021)
   PUBMED   34653670
REFERENCE   3  (bases 1 to 4792)
  AUTHORS   Bedogni F and Hevner RF.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4792)
  AUTHORS   Qiu H, Shi S, Yue J, Xin M, Nairn AV, Lin L, Liu X, Li G,
            Archer-Hartmann SA, Dela Rosa M, Galizzi M, Wang S, Zhang F, Azadi
            P, van Kuppevelt TH, Cardoso WV, Kimata K, Ai X, Moremen KW, Esko
            JD, Linhardt RJ and Wang L.
  TITLE     A mutant-cell library for systematic analysis of heparan sulfate
            structure-function relationships
  JOURNAL   Nat Methods 15 (11), 889-899 (2018)
   PUBMED   30377379
REFERENCE   5  (bases 1 to 4792)
  AUTHORS   Uijtdewilligen PJE, Versteeg EM, van de Westerlo EMA, van der Vlag
            J, Daamen WF and van Kuppevelt TH.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4792)
  AUTHORS   Sedita J, Izvolsky K and Cardoso WV.
  TITLE     Differential expression of heparan sulfate 6-O-sulfotransferase
            isoforms in the mouse embryo suggests distinctive roles during
            organogenesis
  JOURNAL   Dev Dyn 231 (4), 782-794 (2004)
   PUBMED   15499561
  REMARK    GeneRIF: 6OST2 is more mesenchymal than its isoenzymes during
            development
REFERENCE   7  (bases 1 to 4792)
  AUTHORS   Nagai N, Habuchi H, Esko JD and Kimata K.
  TITLE     Stem domains of heparan sulfate 6-O-sulfotransferase are required
            for Golgi localization, oligomer formation and enzyme activity
  JOURNAL   J Cell Sci 117 (Pt 15), 3331-3341 (2004)
   PUBMED   15226404
REFERENCE   8  (bases 1 to 4792)
  AUTHORS   Smeds E, Habuchi H, Do AT, Hjertson E, Grundberg H, Kimata K,
            Lindahl U and Kusche-Gullberg M.
  TITLE     Substrate specificities of mouse heparan sulphate glucosaminyl
            6-O-sulphotransferases
  JOURNAL   Biochem J 372 (Pt 2), 371-380 (2003)
   PUBMED   12611590
REFERENCE   9  (bases 1 to 4792)
  AUTHORS   Ford-Perriss M, Guimond SE, Greferath U, Kita M, Grobe K, Habuchi
            H, Kimata K, Esko JD, Murphy M and Turnbull JE.
  TITLE     Variant heparan sulfates synthesized in developing mouse brain
            differentially regulate FGF signaling
  JOURNAL   Glycobiology 12 (11), 721-727 (2002)
   PUBMED   12460940
REFERENCE   10 (bases 1 to 4792)
  AUTHORS   Habuchi H, Tanaka M, Habuchi O, Yoshida K, Suzuki H, Ban K and
            Kimata K.
  TITLE     The occurrence of three isoforms of heparan sulfate
            6-O-sulfotransferase having different specificities for hexuronic
            acid adjacent to the targeted N-sulfoglucosamine
  JOURNAL   J Biol Chem 275 (4), 2859-2868 (2000)
   PUBMED   10644753
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672057.8, AK052348.1, AL671918.5 and BQ031768.1.
            
            [WARNING] On Dec 6, 2023 this sequence was replaced by
            NM_001077202.3.
            
            On Mar 13, 2014 this sequence version replaced NM_001077202.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.22064.1, AK052348.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139, SAMN01164141
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-221               AL672057.8         22736-22956         c
            222-1348            AK052348.1         2-1128
            1349-3738           AL671918.5         27816-30205         c
            3739-4217           BQ031768.1         18-496              c
            4218-4792           AL671918.5         26762-27336         c
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 28.04 cM"
     gene            1..4792
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /note="heparan sulfate 6-O-sulfotransferase 2"
                     /db_xref="GeneID:50786"
                     /db_xref="MGI:MGI:1354959"
     CDS             1..1959
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /EC_number="2.8.2.-"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="heparan-sulfate 6-O-sulfotransferase 2 isoform
                     1"
                     /protein_id="NP_001070670.1"
                     /db_xref="CCDS:CCDS40970.1"
                     /db_xref="GeneID:50786"
                     /db_xref="MGI:MGI:1354959"
                     /translation="MALPAFAARALGPPLQPEQGAPARTTCPRRHSRVEAELAASRPG
                     SVAASVRAGPPRGVSLGFNSPPLQDKPPKAFSSLAGALRAPLFALLPRGRRRRMHDLR
                     RRWDLGSLCRALLTRGLAAVGHSLKHVLSAIFSKIFGPLASVGNMDEKSNKLLLALVM
                     LFLFAVIVLQYVCPGTECQLLRLQAFSSPVPDPYRSEDESSARFVPRYNFSRGDLLRK
                     VDFDIKGDDLIVFLHIQKTGGTTFGRHLVRNIQLEQPCECRVGQKKCTCHRPGKRETW
                     LFSRFSTGWSCGLHADWTELTSCVPAVVDGKRDARLRPSRWRIFQILDGTSKDRWGSS
                     NFNSGANSPSSTKPRSTSKSGKNFHYITILRDPVSRYLSEWRHVQRGATWKASLHVCD
                     GRPPTSEELPSCYTGDDWSGCPLKEFMDCPYNLANNRQVRMLSDLTLVGCYNLSVMPE
                     KQRNKVLLESAKSNLKHMAFFGLTEFQRKTQYLFEKTFNMNFISPFTQYNTTRASSVE
                     INEEIQKRIEGLNFLDMELYSYAKDLFLQRYQFMRQKEHQDARRKRQEQRKFLKGRFL
                     QTHFQSQSQGQSQSQSPGQNLSQNPNPNPNQNLTQNLSHNLTPSSNPNSTQRENRGSQ
                     KQGSGQGQGDSGTSNGTNDYIGSVETWR"
     exon            1..428
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /inference="alignment:Splign:2.1.0"
     exon            429..947
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /inference="alignment:Splign:2.1.0"
     exon            948..980
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1067
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..4792
                     /gene="Hs6st2"
                     /gene_synonym="6OST2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 atggcactgc ctgcgtttgc agcccgggcg ctcgggccgc cactgcaacc ggagcaagga
       61 gcgcccgccc gcaccacctg tccccgccgg cattccagag tagaggccga attggcagca
      121 agccggcccg ggtctgttgc cgcctcagtc cgcgccggcc ctcctagggg tgtgtctctc
      181 ggattcaact ccccgccgct ccaggacaag cccccaaagg cattttcttc cttggcggga
      241 gccttgcgcg ccccgctctt cgcgctgctg cctcgaggcc gccgcaggcg gatgcacgac
      301 ctcaggcgac gctgggacct gggctccctc tgccgggccc tgctcactcg gggcctggca
      361 gccgtgggcc actcgctcaa gcacgtgctc agtgcgatct tctccaagat tttcggtccc
      421 ttggccagcg tcgggaacat ggatgagaaa tctaacaagc tgctgctggc tttggtgatg
      481 ctcttcctat ttgcggtgat cgtcctccaa tacgtgtgcc ccggcacaga atgccagctc
      541 ctccgcctgc aggcgttcag ctccccggtg ccggatccgt accgctcgga ggatgagagt
      601 tcggccaggt ttgtaccccg ctacaatttc agccgcggcg atctcctgcg caaggtagac
      661 ttcgacatca agggcgatga cctgatcgtg ttcctgcaca tccagaagac tgggggcacc
      721 acttttggcc gtcacctggt gcgcaacatc cagctggagc agccatgtga gtgccgcgtg
      781 gggcagaaga aatgcacttg ccaccggccg ggtaagaggg agacctggct cttctccagg
      841 ttctccaccg gctggagctg cgggctgcat gccgactgga ccgagctcac cagctgcgtg
      901 ccggcggtgg tggatggcaa gcgcgacgcc aggctgagac cttccaggtg gaggattttt
      961 cagattctag atggaacaag taaggataga tggggttctt caaacttcaa ctcaggcgcc
     1021 aactctccat cctccacaaa gccccggagc acatcgaaga gtgggaagaa cttccattac
     1081 attaccatcc tgagagaccc agtgtcacgg tacttgagtg aatggaggca tgtccagaga
     1141 ggagcaactt ggaaagcatc cctgcacgtc tgtgatggaa ggcccccaac ctctgaagag
     1201 ctgcccagct gctacaccgg tgatgactgg tctggatgcc ctctcaaaga gttcatggac
     1261 tgtccctata atctggccaa caaccgccaa gttcgcatgc tatctgacct gactctagtg
     1321 ggatgctaca acctctctgt catgcctgaa aagcaaagaa acaaagtcct tctggaaagt
     1381 gccaaatcca atctgaagca catggcgttc tttggcctca ctgagtttca gcgcaagacc
     1441 cagtacctgt ttgagaagac cttcaacatg aactttatct cgccgtttac ccagtataat
     1501 accactaggg cctctagtgt tgagatcaat gaggaaatcc aaaagcgtat tgagggactg
     1561 aattttctgg atatggagtt gtacagctat gctaaagacc tctttctgca aaggtatcag
     1621 ttcatgaggc agaaagaaca tcaggatgcc aggcggaagc gtcaggagca acgcaaattt
     1681 ctgaagggaa ggttccttca gacccatttc cagagtcaga gtcagggtca gagccagagc
     1741 cagagtccag gtcagaatct gagtcagaat ccaaatccta acccaaatca gaacctgact
     1801 cagaacctga gtcacaatct gactccgagt tcaaatccca attcgaccca gagggagaac
     1861 cggggaagtc agaagcaggg ctcaggccag ggacaaggtg atagcggcac cagcaatggc
     1921 accaatgact acatagggag cgtagagaca tggcgctaac tggcactcaa aggcttgtgc
     1981 acactgcttc ccaaagcatc accaaaaaga tggcgtgtat cttacaagat gaaaatgtcc
     2041 aaacacatcc tgcttccttc atttgggaag ttcaaaaaaa aaaagtgtag acgttgcctt
     2101 tacagttgcc ttttgattca gtgttatgct gtgtgtgggt acaacaaatc tcagtgtgta
     2161 attaaattgt ctgttttggg atgaactact tctgaaatcc aagagccaaa ccagactcac
     2221 cagaaattgc agcttagata ttttaagacg ttcttaaatt aggtatggga gacacaaaga
     2281 aaacatgaaa tgtggccgtt taaacttatg gctaagaaac agactttaag tgattccgga
     2341 tactctgtta aaactcagtc ttgaaagcac cccccctccc tgcagggaag tataagtaaa
     2401 aacatgaaca gaactaaaag acactcaaaa ctgttcattt ccttgtttta acaaggagcc
     2461 tatttaaaca gaaaaacaac tgatgataaa ttttgccaaa ctgtagaaaa taaacaattt
     2521 ctcacttaaa tgtagctggg tttatgtaaa aataatggct tttcaaatag atcgggattc
     2581 gtgtcttgta acttaacaga tgtttaaaat ttagaatttt tctaccttct gctgattaaa
     2641 aggttttaaa cagggtatat ctcatattaa caaagcattt tttccaaatg gaataccaat
     2701 ggaaagatcc agtttccaga tggtttctgg gcactgtaat tccaacaatt tcgatctttt
     2761 tggcgctgct tctcattcac tttaaggctt ctccgagttg tgctcattcc aaatgaatcc
     2821 atgtatagaa tttttcttct tcatctaaac aacgtgttgc taaatctctt gtgctattta
     2881 atcctggact aaagtcagga ctttctatag aattgtctta tcaatgtctg tgtagtaagg
     2941 tccttaagaa gaactctcaa acagaataat ttgtctctta atattgctga agttattatt
     3001 cttcatcaga ttgaactaaa cctgaatgtg aactgttaat tatcttgttt attgtaatgt
     3061 ttccagtttg gctggctatt tctggcccat ggttcttttc ttgtattgac agatttgtac
     3121 acaataataa gagcaaaaaa ttttctcaaa gacaacagta gactaatcat taagatagcc
     3181 agagttgctc aataacacca aaagctgcct cttgataatt gaggggaaca cttctaccag
     3241 gattttgttt gtgaaagatc aaaaataaag aatgttttgc agcgaagcta agtgtcaggg
     3301 acgtgcaagt agaggagaat agtttgatag tctgcatctg tgaagagtat tttgatggtt
     3361 tcaattacaa atggaacaaa ggaagaatta tatacacact gtgttgtgaa atatcatact
     3421 gcttggcagt ttacacctca tggttgttgc tacacttcta taagagctta cattaatccc
     3481 ttggctttca atcaaaacaa ggtgaccaga agtggtattc atgctttcaa gagggacatg
     3541 cttagaaata tgatcataaa gtcacatctg tcatagtctg cccaagtgca tgacctggtt
     3601 tcatctgtaa taatatggcc acaagtttgg caagaagttg agtatctggc tctaaagaga
     3661 ttttcttgac tctgagctct gtgacaatat ttgcagcttt tgttttgtgg tatttgcagt
     3721 gttgagaatg atgataccta aaaaaaaatc aaaaaacaaa aacaaaaaaa tgaagaaaaa
     3781 acaaaaacaa acaaaaaaaa actggaagga atttttaaaa gcacatcttg aagtcaaagt
     3841 atttggtgta cattgtgttc ttgaaaactt ctgtagatca ctatggtatt tcagtaattt
     3901 tagttttaat tgaaaatgat tgttcttaaa actgacagtg ttggaaagca ggacgaagtc
     3961 tgagccagtg gaaaagctca ttacagaaaa aaaaaagtgt tattgctgtg gtgtgcatgg
     4021 tttgcagaga ttaagtgcat tttctctgtc tctactgatt attgtatata gagaatgtta
     4081 taaatataca tttttgtcat catcatgtaa atcccacgat ttcaaactgt aaacatctgt
     4141 tcagtggtgt agctttacaa actgttcact gattttgtgt aatttatcaa tagatgtgaa
     4201 ataaagtttc aattgcaggc tgtgagtaat ctcatgattt caaggtttat agccagtaga
     4261 cattgtgata ccataaacaa accagaacag gaatcgtttc aaagacagtg tttttcttta
     4321 cacttgacag ctaagtatga aaatgagatg gcccatttta atactcatgc tgaaatggcc
     4381 ttaatttggg atgctttttc cttggaggcc atgatttgaa taaaacaaaa ttgttcctga
     4441 aataaggttt gccgtgcaaa tgaagtgaac ccacgttctc atttaaagtg tatatagtag
     4501 atgctttcat atatcataga atgcacaaag ggaaaaaaat agcactctta attacttacc
     4561 tccttttatc cacaagaaaa aggttaaagg taagcattaa tattttggcc ttggggtctg
     4621 gagcagttaa gagcactgac tgctcttctg gaggtcctga gttcaattcc cagcaaccac
     4681 atgggaatga accccagctc acagccatct gtaatgggat ctgatgccct cttctggtgt
     4741 gtcggaagat acctacagtg tactcatata aataaaataa atttgacaaa ac
//
