LOCUS       NM_001253356            3063 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus heparan sulfate (glucosamine) 3-O-sulfotransferase 5
            (Hs3st5), transcript variant 2, mRNA.
ACCESSION   NM_001253356
VERSION     NM_001253356.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3063)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3063)
  AUTHORS   Chen,J., Duncan,M.B., Carrick,K., Pope,R.M. and Liu,J.
  TITLE     Biosynthesis of 3-O-sulfated heparan sulfate: unique substrate
            specificity of heparan sulfate 3-O-sulfotransferase isoform 5
  JOURNAL   Glycobiology 13 (11), 785-794 (2003)
   PUBMED   12907690
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ113442.1, BC147493.1 and BC079867.1.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' UTR
            compared to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.55243.1, BC147493.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-7                 CJ113442.1         2-8
            8-2415              BC147493.1         1-2408
            2416-3063           BC079867.1         2092-2739
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 19.31 cM"
     gene            1..3063
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /note="heparan sulfate (glucosamine) 3-O-sulfotransferase
                     5"
                     /db_xref="GeneID:319415"
                     /db_xref="MGI:MGI:2441996"
     exon            1..809
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /inference="alignment:Splign:2.1.0"
     exon            810..975
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1080
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1219
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /inference="alignment:Splign:2.1.0"
     CDS             1113..2153
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /EC_number="2.8.2.23"
                     /note="heparan sulfate D-glucosaminyl 3-O-sulfotransferase
                     5"
                     /codon_start=1
                     /product="heparan sulfate glucosamine 3-O-sulfotransferase
                     5"
                     /protein_id="NP_001240285.1"
                     /db_xref="CCDS:CCDS35881.1"
                     /db_xref="GeneID:319415"
                     /db_xref="MGI:MGI:2441996"
                     /translation="MLFKQQVWLRQKLLVLGSLAVGSLLYLVARVGSLDRLQPICPVE
                     SRFGGAHNQAELPLRALQFKRGLLHEFRKGNSSKEQVHLHDLVQQLPKAIIIGVRKGG
                     TRALLEMLNLHPAVVKASQEIHFFDNDENYAKGIEWYRKKMPFSYPQQITIEKSPAYF
                     ITEEVPERIYKMNSSIKLLIIVREPTTRAISDYTQVLEGKERKNKTYYKFEKLAIDPN
                     TCEVNTKYKAVRTSIYTKHLERWLKYFPIEQFHIVDGDRLITEPLPELQLVEKFLNLP
                     PRISQYNLYFNATRGFYCLRFNIIFNKCLAGSKGRIHPEVDPSVITKLRKFFHPFNQK
                     FYQITGRTLNWP"
     exon            1220..3039
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3005..3010
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /note="hexamer: ATTAAA"
     polyA_site      3026
                     /gene="Hs3st5"
                     /gene_synonym="D930005L05Rik; Gm1151; Hs3ost5"
                     /note="major polyA site"
ORIGIN      
        1 gggatgagag ctgggagggt gtggactggc agccactctg cagctggccg ccagggacct
       61 ggtccctccc gcgagccgaa gtgatggagg ggaagatatc ttcttagaca tacgcgcgcg
      121 cgcacatccc gctgggcacg tctccgggag gctgcagccg gctccagcgc gccagggacc
      181 gggcggcggc tgtgacagcg cgagaggcgg ggagtccgcg gtccgcacag cttggcacag
      241 gccaccggag tacgcaggga aggaagatct cagcctcact gactgttgct cttttggagg
      301 catctccaag gaccgagctt tctgagattg tcactgcgga cgcccgaggc tttgcccctc
      361 gcctgcggag tccgggtcag cgtcggtgcc cgcgccgggc gaggagaatg cgaacctcct
      421 cccgcagagc tcctgcggtc cccttcactg cgcgaactaa ggctcccgca gctgcccctg
      481 gcgtgctcgc gccgcgggag cgcgccctgc cgcacctggc tccagtctcc tagggaagtg
      541 cgggaaacgt gccctaaact ccactacttc tcttcccctc ctttggcttg cagctccccg
      601 cccaggacac catagccgcc tctgtcgccg cccgagtgga agcccgggta ctgtgctgct
      661 tgtttgctct gctcgccggc ttgctcctag ctgagcatcc caaactactg gacaactgcc
      721 actgcgatcg cgggaaccac ttggtgaccc ggggcgactg gcctgggcca ccactttcca
      781 agccgaggct gtcaccacta gggacgaaga gataaccagg ccccactggc tttagactgg
      841 tgctttctgc tgcccacctc aggatcttca acagccagct gacgctctca gaaactagac
      901 aagcaacttg tcatattctt ggaagtctct cctccataca tacagaactt cctggatgag
      961 cataaagatg tgaaggtgct cacaaggagg aacaggacaa ctaggaggtg aggagaggcc
     1021 atgagacaac cactagccag ataatcctct cagaagtaac tgaggcgtgt ctgaatgtag
     1081 gctgctgcag ttctgaaggt tgatggaggg ccatgctatt caaacagcag gtgtggctga
     1141 gacagaagct cctggtactg ggaagccttg ctgttgggag cctcctgtat ctagttgcca
     1201 gagttgggag cttggatagg ctccagccca tttgccctgt tgaaagtcga tttggtggtg
     1261 cccacaacca ggctgagttg ccactgcggg ccctgcagtt taagagaggc ttgctgcatg
     1321 agttccggaa gggcaattct tccaaggagc aggttcacct ccatgacctg gtccaacagc
     1381 tccccaaagc cattatcatt ggggtgagaa aagggggcac aagggccctg ctagagatgc
     1441 tcaacctcca tcctgcagtg gtcaaagctt cccaagagat ccacttcttt gacaatgatg
     1501 agaattatgc caagggcatt gagtggtaca ggaaaaagat gcctttttcc taccctcagc
     1561 aaatcacgat tgaaaagagc ccggcatatt tcatcacaga agaggttccg gaaaggattt
     1621 acaagatgaa ctcatccatc aagctgttga tcattgtcag ggagccgacc acaagagcaa
     1681 tttctgatta tactcaggtg ctagagggga aggagcggaa gaacaaaacc tactataagt
     1741 ttgaaaaact ggctatagac cctaatacct gtgaagtgaa cacgaaatac aaggcggtta
     1801 ggaccagcat atacacaaaa catctggagc gctggttgaa atactttccc attgaacagt
     1861 ttcatatcgt agatggtgac cgtcttatca cagaacctct gccggaacta cagctggtgg
     1921 aaaagttctt aaaccttcct ccgaggataa gtcagtacaa tttatatttc aatgctacca
     1981 gagggtttta ctgtctgaga tttaacatta tctttaataa gtgcctggcg ggcagcaagg
     2041 ggcgcatcca tccagaggta gacccctccg tcattaccaa attgcgcaaa ttctttcatc
     2101 cttttaatca aaaattttac cagatcactg ggaggacatt gaactggccc taaaataata
     2161 tagcaaacaa caccatgtgt tgtatctaga gacgcatggc gtcccctcta cattaaaata
     2221 tgcactttcc cagacacagc tccccaattc atttttccat aaatacttgt acatatgcag
     2281 tgtgtgacca aatatgagat caattgtttt tctactgaaa ctttacaaaa aataaataat
     2341 aaatgactgt ctacatggtt gcatctgtta agctatttgc agaaaggaaa ggaaaggaaa
     2401 cggaagaggt ggtgggactg tagaaagtga cctcagatat tctcaagtta gtcttccttt
     2461 gagaaaattc ctttgactta gaacttgtca cccaccattg tcagatttaa gccaaaagat
     2521 gagatgtgaa aatgtaccaa tggctgcgca gcttcaggaa ttagaggatt cagttttatg
     2581 attcatttgc ctgtttgttc gtttgttttg tttgttttgg tttggttttg tcctaaacta
     2641 acattggctc tttaattcaa acactggatt ggtgccatga aaacagaata cgctattttc
     2701 ttataattta aaagaatgtc ttagctcaga aaattaacac tgttccaaag ttcttgtggt
     2761 gattttgcac tatagtttcc tggtatgttc catggtgtca tttgtcgcat gtcaatcacg
     2821 tgctggaaaa tcaccacatt cttttacttc catgtttgta tgtcaacaaa gaaaaatgtc
     2881 ctgtagatat gtgtaaatga tgtaaatact cgtttcacac taagtaattc tattttgtaa
     2941 actgaatatg gctatttaat ttattgtgaa aattaaattt attgtggtat ttaaaacgga
     3001 atggattaaa attacacaat gtgcaacttt ccttaaaaaa aaaaaaaaaa aaaaaaaaaa
     3061 aaa
//
