LOCUS       NM_011828               6217 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus heparan sulfate 2-O-sulfotransferase 1 (Hs2st1),
            transcript variant 2, mRNA.
ACCESSION   NM_011828
VERSION     NM_011828.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4.. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  AUTHORS   Tao,C., Makrides,N., Chuang,J.Z., Wu,Y., Brooks,S.E., Esko,J.D.,
            Sung,C.H. and Zhang,X.
  TITLE     Chondroitin sulfate enhances the barrier function of basement
            membrane assembled by heparan sulfate
  JOURNAL   Development 149 (12) (2022)
   PUBMED   35608020
REFERENCE   3  (bases 1 to 6217)
  AUTHORS   Patel,V.N., Pineda,D.L., Berenstein,E., Hauser,B.R., Choi,S.,
            Prochazkova,M., Zheng,C., Goldsmith,C.M., van Kuppevelt,T.H.,
            Kulkarni,A., Song,Y., Linhardt,R.J., Chibly,A.M. and Hoffman,M.P.
  TITLE     Loss of Hs3st3a1 or Hs3st3b1 enzymes alters heparan sulfate to
            reduce epithelial morphogenesis and adult salivary gland function
  JOURNAL   Matrix Biol 103-104, 37-57 (2021)
   PUBMED   34653670
REFERENCE   4  (bases 1 to 6217)
  AUTHORS   Li,M. and Xu,D.
  TITLE     Antiresorptive activity of osteoprotegerin requires an intact
            heparan sulfate-binding site
  JOURNAL   Proc Natl Acad Sci U S A 117 (29), 17187-17194 (2020)
   PUBMED   32636266
REFERENCE   5  (bases 1 to 6217)
  AUTHORS   Merry,C.L. and Wilson,V.A.
  TITLE     Role of heparan sulfate-2-O-sulfotransferase in the mouse
  JOURNAL   Biochim Biophys Acta 1573 (3), 319-327 (2002)
   PUBMED   12417414
  REMARK    Review article
REFERENCE   6  (bases 1 to 6217)
  AUTHORS   Pinhal,M.A., Smith,B., Olson,S., Aikawa,J., Kimata,K. and Esko,J.D.
  TITLE     Enzyme interactions in heparan sulfate biosynthesis: uronosyl
            5-epimerase and 2-O-sulfotransferase interact in vivo
  JOURNAL   Proc Natl Acad Sci U S A 98 (23), 12984-12989 (2001)
   PUBMED   11687650
REFERENCE   7  (bases 1 to 6217)
  AUTHORS   Merry,C.L., Bullock,S.L., Swan,D.C., Backen,A.C., Lyon,M.,
            Beddington,R.S., Wilson,V.A. and Gallagher,J.T.
  TITLE     The molecular phenotype of heparan sulfate in the Hs2st-/- mutant
            mouse
  JOURNAL   J Biol Chem 276 (38), 35429-35434 (2001)
   PUBMED   11457822
REFERENCE   8  (bases 1 to 6217)
  AUTHORS   Rong,J., Habuchi,H., Kimata,K., Lindahl,U. and Kusche-Gullberg,M.
  TITLE     Substrate specificity of the heparan sulfate hexuronic acid
            2-O-sulfotransferase
  JOURNAL   Biochemistry 40 (18), 5548-5555 (2001)
   PUBMED   11331020
REFERENCE   9  (bases 1 to 6217)
  AUTHORS   Rong,J., Habuchi,H., Kimata,K., Lindahl,U. and Kusche-Gullberg,M.
  TITLE     Expression of heparan sulphate L-iduronyl 2-O-sulphotransferase in
            human kidney 293 cells results in increased D-glucuronyl
            2-O-sulphation
  JOURNAL   Biochem J 346 Pt 2 (Pt 2), 463-468 (2000)
   PUBMED   10677367
REFERENCE   10 (bases 1 to 6217)
  AUTHORS   Bullock,S.L., Fletcher,J.M., Beddington,R.S. and Wilson,V.A.
  TITLE     Renal agenesis in mice homozygous for a gene trap mutation in the
            gene encoding heparan sulfate 2-sulfotransferase
  JOURNAL   Genes Dev 12 (12), 1894-1906 (1998)
   PUBMED   9637690
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC123880.18, BC059008.1 and AC159976.4.
            
            On Sep 20, 2017 this sequence version replaced NM_011828.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088698.1, BC059008.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164137
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-243               AC123880.18        37987-38229
            244-4252            BC059008.1         1-4009
            4253-6217           AC159976.4         112619-114583       c
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 68.89 cM"
     gene            1..6217
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /note="heparan sulfate 2-O-sulfotransferase 1"
                     /db_xref="GeneID:23908"
                     /db_xref="MGI:MGI:1346049"
     exon            1..483
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     CDS             360..1430
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /EC_number="2.8.2.-"
                     /note="isoform 2 is encoded by transcript variant 2;
                     2-O-sulfotransferase"
                     /codon_start=1
                     /product="heparan sulfate 2-O-sulfotransferase 1 isoform
                     2"
                     /protein_id="NP_035958.3"
                     /db_xref="CCDS:CCDS17883.1"
                     /db_xref="GeneID:23908"
                     /db_xref="MGI:MGI:1346049"
                     /translation="MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRAKLERA
                     IARHEVREIEQRHTMDGPRQDATLDEEEDIIIIYNRVPKTASTSFTNIAYDLCAKNRY
                     HVLHINTTKNNPVMSLQDQVRFVKNITTWNEMKPGFYHGHISYLDFAKFGVKKKPIYI
                     NVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQI
                     PFFCGHSSECWNVGSRWAMDQAKSNLINEYFLVGVTEELEDFIMLLEAALPRFFRGAT
                     DLYRTGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFIRAHAVRE
                     KDGDLYILAQNFFYEKIYPKSN"
     exon            484..722
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     exon            723..808
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     exon            809..947
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1045
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     exon            1046..1203
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..6217
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2449..2454
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /note="hexamer: AGTAAA"
     polyA_site      2475
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /note="major polyA site"
     regulatory      4216..4221
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /note="hexamer: AGTAAA"
     polyA_site      4252
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
     regulatory      4791..4796
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
                     /note="hexamer: AATGAA"
     polyA_site      4815
                     /gene="Hs2st1"
                     /gene_synonym="2OST; Hs2st; mKIAA0448"
ORIGIN      
        1 gagggcggcg cagccgcagc cgcatcgcca ccggaggagc gcgcggccgc gagcgaccga
       61 gggagggaag ggaggaagcg agggaagagg gcgtgcggcc gtggggtccc gaagccaggg
      121 ggcagcggca gcggcagcgg caggagggcg ccagggccgt cgagtgggcc ggggagaagc
      181 gtgcaccagg cggccggcgc tggctcccgc gggcgggaga ccggagagga ggagggggtc
      241 gctgcggcgc ggcagtccgc gcagctcccg ggtcggggtc tcctcccggc gcttcggtcg
      301 ccctcggggc cccgctcccc gccccccgcg gtatgtcttg atcgcgagcg gcgggtttca
      361 tggggctcct caggattatg atgccgccca agttgcagct gctggcggtg gtggccttcg
      421 cggtggcgat gctcttcttg gagaaccaga tccagaagct ggaggagtcc cgggcgaagc
      481 tagaaagggc aattgcaagg cacgaagtcc gagaaattga acagcgccat acaatggatg
      541 gccctcggca ggatgcaact ctggatgaag aagaagacat aatcatcatt tataacagag
      601 tgcccaaaac tgcaagcacc tcgttcacca atatagccta cgacctgtgt gcaaagaata
      661 gataccatgt tcttcacatc aacactacca aaaataaccc agtaatgtca ttgcaagatc
      721 aggtgcgctt tgtgaagaac ataaccacct ggaacgagat gaagccaggc ttctatcacg
      781 gacacatctc ttacttggat tttgcaaaat tcggtgtgaa gaagaaacca atttatatca
      841 atgtcatcag ggaccctatt gagaggctag tttcctacta ttacttcctg aggtttggag
      901 atgattacag accaggatta aggagacgga aacaaggaga caaaaagacc ttcgatgaat
      961 gtgtggctga gggcggctcc gactgtgctc cagagaagct ctggcttcag atcccgttct
     1021 tctgtggcca cagctcagaa tgctggaatg tagggagcag atgggccatg gaccaggcta
     1081 agtctaacct cattaatgag tacttcctgg tgggagtcac cgaggagctc gaggacttca
     1141 tcatgctgct cgaggcagct ttgccccgat tcttccgggg cgctaccgac ctctaccgta
     1201 caggaaagaa atctcacctt aggaaaacca cagagaagaa gctgcccacc aagcagacta
     1261 tcgcgaagct gcagcagtct gacatttgga agatggagaa tgagttctat gagtttgctc
     1321 tggagcagtt ccagttcatc cgagctcacg ctgtccgcga gaaagacgga gacctctaca
     1381 tcctggccca gaactttttc tacgaaaaga tttaccctaa gtcgaactga gcgcaggtgt
     1441 ggccagagca ctcctgcccc ggaacttgac tgtgtcacca cccttctcag ccccctgctg
     1501 atgcgtgtgc gagctgtgag ccacgctgag ctggggacag acatgaatgt caaggagtga
     1561 gatgctggcg ggctggcgga gtcctgtgga gtttttaact ttctggcatt tttacttttt
     1621 tgttgtttta ttttcgtttt catgatagtt ataatcttct cctggtgcag ggacatgggg
     1681 acttacattg cctaaaatac acacatggac gtttagtcag atggctgaat accatttcag
     1741 aagaggtttg cataagcatt tctttgcctc tccttgaatg aagaggagtc tgtttgccac
     1801 ttacccttct ggagtttgtg ccgccacctc tgctggatag tgctaaaata tgtttggcag
     1861 tatctgattt atttgtatga atcatgtctc acaaagttta ttagaatgtt tgatcgtgtt
     1921 ttcccagaac atttctgcta cagttgggtt tgcccatatt tatgtaggct ttattttatt
     1981 ttatttgatg atcattagtg ttaaagaaat caacttaaac catgaataat actgtaagaa
     2041 ggtaaaaagt taaagcagta ttcctgactc ctgtcacccc agtatccaat attggaatga
     2101 tatttctaag aatgtggaca ttatctgagg ctttcttaag gatttccaca tatcaatatc
     2161 aaaaaattga gtttaatact tgttccttca tggcgtatct gtacccctga acactggcat
     2221 attggtgacc gcacatggcg actccatcag tgagctgtga tgtgggtagg atttgcctac
     2281 tctgtacttt tacctgtaga ctatttttac tacggtgctt tataatgtgt tttaaagcat
     2341 tgcatttaca aaaggaaagt gctgtaaata ttgcatattt tatgtatttg gaccaaaaag
     2401 ttacaagtaa gtagacaaca gtggtgttgc acccgtttta ttatgtcgag taaagtcgtc
     2461 agacctccta tcctattctg gtatttctcg tttaacgtta ctgttaggat gtcactaaaa
     2521 tgagctaacg tagcctttca agtttgatag agaacagtgc tcacccctga ggcagtagtg
     2581 gtcatagaaa aggtcgtaga gtctgagggt tccatctctg gttccagctc tgctgtcacc
     2641 ttcctgctgt gtgacgttag gaagtcacct gacctccctg cctcagcttc ctcatcttga
     2701 tatcaggaca ctaagacctt ctctccctgc ctcacaggga tgttgtgagg actaaggaag
     2761 atggcatggc gaagagcact ttaaaactgt ggcgtggtgt tcaaatgtga ggtgttgtca
     2821 cagaagtatc tctagctgat agtttcatag tctctatttt agcaacaaaa agggaaaatc
     2881 cacttttaag ctttatgaga aaaaaaaccc cttcctcttg atataaattt gtgtttgata
     2941 aatatcttct cagtgtttta tcttctgtgc ttaagcaact attgaagtgt gaacggctta
     3001 tgcactcgag agactcagac cgtcactagc tgaggagcag gaaagccttc tataaacttg
     3061 aaggatgttc ttgtttgtat ctaactttgt catccgaaaa tggtggctat tttacacttg
     3121 tgcttgttaa agtgtccttt gtagaattta atttctctat taattcttca cctactccct
     3181 ctttacagcc aggattcaga ttcctctcag acacactttt ctaaaggagt aattatttgt
     3241 atctttaaat gtttctaaac aggacctcat caaacaaagc attttcaaca gaagacttta
     3301 aagtatttat tgtgtaatag gaggagtgaa ggacactgta tatcagaaag cacttaggaa
     3361 gattaaagga acgactgagt cacaggctcc gccgagatga aagtattagg ggaacatagc
     3421 aacgtttccc ttccccctcc gtcctttcca gtgcaagttt ctaggtcctg ctaaaagcaa
     3481 gttgttttct tgtccaggca ggcatctggc tgttgagtaa atctgaggtt attcttgaga
     3541 gttcgcacta aactcttagg tagttaaggc agcacttaga acactaactg ggactttccc
     3601 taaactgtcc tcataaaaga ccaggtaatg agtcaggtct ctgtacagct cttactgttg
     3661 gaaagtcgct gtcacagaca ggagaggcaa agggccgtgg ttagagctta gcaaaacctt
     3721 tatttacaga acagaaggca tactagatat gaaccatgag ccgcaagccc tcgctcaccc
     3781 tgtgagggat tgtcatggag aagccctgtc tgtgtccgga ccacgttccc caaagagttc
     3841 ctctacctcc catgtctccc agatcgtgac cccaaggact gctttctgac tcagtccctc
     3901 cccagcttcc ttctgtctta ccttgctgtc aggtgactcg ttttcctagc tcacctgtac
     3961 agattcggga catttctttt atcgcttttt ggagcgaatg gattccaagt gtcctttctg
     4021 gagactaaga aaagggtggg cttgcttctc taagagtcct gggagcctga gtccttagaa
     4081 catataactg tgtaatcaaa tgaatagaac cactgagaca atgtgtattt tttacatgtg
     4141 gcaaatgctt ttcttttttt ggcctttgtg ctttttcagt aatttgacct tagaaaaaaa
     4201 tggtttttat atgatagtaa agcaaaataa ccactttgaa ttttaacgat aatgtcaatg
     4261 tgtgtgtgtg tctatgttta tttcctacag aagaagaggt agctttgtgt gttcagcttg
     4321 taccaaccgt cttcttattg ctgagaaatg gccactattg ataaaccttt ctgttttaaa
     4381 atgtagtggg gaggttatgt tttgatctcc tctggcttta atttctagtg tcacttgaat
     4441 atatctggta gcaaaccttg aagcaccgca taggagctgc ttctgtgtcc cctgatagtc
     4501 acacagtccc taacatccgc tgcttctggc ctcctgagca ttggatttaa atatccaaaa
     4561 ccctcaaaac tccaagttgc cagcttttta aacacttgct tttcccttct gtgctgaaaa
     4621 tccagatagc acctataatt tggactctat tgatcttcct cattatgtgt cctgttggaa
     4681 acccctaaaa ttgtctgctc catcagggaa cttaccagag tattttgatg ttgtatttgc
     4741 atagatggat atgaaatagt ttatagatga caattctctt ttttgaataa aatgaaattt
     4801 ttgaaaaatc taaaagtact agagaactta tctaaagccc agttccctaa tgcatagcta
     4861 gtgttttaat ttatgttaaa attctgctca gagaccatct gtgtaagtca tcttgccagc
     4921 tttgccagcc atcattttca aacatagcta tggtcccagg agcctttatt atgggagtat
     4981 ctggaaccta agacttctat gaaacttgtg ttttaggaac tcaaatgctt ctgcttcgtg
     5041 acatactagt gccattgctg tcaagtcttc gcatcgttgt accttggaca gagttgcttt
     5101 gtgcaggggg tgtgggctgg gggagtacag acagactgct ctgcgggcag tcttctgaga
     5161 ggaaagacat tcaaggggct gggtggccat ctggtcacat tcatcctata ctttaatgcc
     5221 atgtggcttg atgacctttg cagtgctatt tctggaatgg accctgattg ttatgacctg
     5281 ttaaaggtga actctcgagc catgtagaca tgctgttaca gcctgtccag agtgaggtca
     5341 tgggagcagc tctaagtcag ggtccaatgt agcttagaga cacatacatg gtgaatggcg
     5401 gctttagcta atcatgccca ccccttccct gccctctgtc ccctttcccc tctcctcccc
     5461 tctccctgcc ctctctaccc accgctgtca tttatgctac actgaccaac ttaaaacgac
     5521 tttgtcacat cccttagaga gacagtcacc ccctttcctc agcattctta caacgctgtg
     5581 ccgcttaagt gttcctgtct gatacctgga agaaaagcta tacaatcaac actgcaggaa
     5641 gagggaatgt ggctccacgg ctgcgtgtcc tcgtggaacg ttttcttctg ctgctttgat
     5701 gctaaggtga cttggtgttt ggtgtcacct gagagctcag atactgtcac cagaaagccc
     5761 tgggtcatca cccatctaca gagctttgct agcttaattt ttgacaagtt tgtatgcgtt
     5821 ttgtctttgg ttgccttgtg tttattagtc atcggtgttg acatttcaaa ttggagatat
     5881 ataaggtata cttaggcatt cagttagcaa gaggaagaaa taaaatggga gcaggctgtt
     5941 gggataagag gatggggtgt gcaggcctgg ttagcacttg gagctctctg tcttcagggc
     6001 agcccatggc ccttacatcc tgagaacaac agcagcgcca ccgcagagat cgccacaagt
     6061 tcagcatttt gttggttttt taaagattta ttaactttag actttctctg ggtctgaagc
     6121 cattctggtc agtagagtaa gttccaggac tacatagtga aactctgtct ttaagaaaaa
     6181 tcaaaaccaa gaaataaatt ttactaattt tgtctgg
//
