LOCUS       NM_001372357            2772 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus hook microtubule tethering protein 2 (Hook2),
            transcript variant 6, mRNA.
ACCESSION   NM_001372357 XM_006530736
VERSION     NM_001372357.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2772)
  AUTHORS   Quesnel-Vallieres,M., Irimia,M., Cordes,S.P. and Blencowe,B.J.
  TITLE     Essential roles for the splicing regulator nSR100/SRRM4 during
            nervous system development
  JOURNAL   Genes Dev 29 (7), 746-759 (2015)
   PUBMED   25838543
REFERENCE   4  (bases 1 to 2772)
  AUTHORS   Liu,Y., DeBoer,K., de Kretser,D.M., O'Donnell,L., O'Connor,A.E.,
            Merriner,D.J., Okuda,H., Whittle,B., Jans,D.A., Efthymiadis,A.,
            McLachlan,R.I., Ormandy,C.J., Goodnow,C.C., Jamsai,D. and
            O'Bryan,M.K.
  TITLE     LRGUK-1 is required for basal body and manchette function during
            spermatogenesis and male fertility
  JOURNAL   PLoS Genet 11 (3), e1005090 (2015)
   PUBMED   25781171
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2772)
  AUTHORS   Moynihan,K.L., Pooley,R., Miller,P.M., Kaverina,I. and Bader,D.M.
  TITLE     Murine CENP-F regulates centrosomal microtubule nucleation and
            interacts with Hook2 at the centrosome
  JOURNAL   Mol Biol Cell 20 (22), 4790-4803 (2009)
   PUBMED   19793914
  REMARK    GeneRIF: Results identify Hook2, a linker protein that is essential
            for regulation of the microtubule network at the centrosome, as a
            binding partner of CENP-F.
REFERENCE   6  (bases 1 to 2772)
  AUTHORS   White,R.A., Sokolovsky,I.V., Britt,M.I., Nsumu,N.N., Logsdon,D.P.,
            McNulty,S.G., Wilmes,L.A., Brewer,B.P., Wirtz,E., Joyce,H.R.,
            Fegley,B., Smith,A. and Heruth,D.P.
  TITLE     Hematologic characterization and chromosomal localization of the
            novel dominantly inherited mouse hemolytic anemia, neonatal anemia
            (Nan)
  JOURNAL   Blood Cells Mol Dis 43 (2), 141-148 (2009)
   PUBMED   19409822
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163703.4.
            
            On Aug 30, 2019 this sequence version replaced XM_006530736.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.152882.1,
                                           SRR11927938.2138734.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-130               AC163703.4         170549-170678       c
            131-216             AC163703.4         169997-170082       c
            217-289             AC163703.4         169852-169924       c
            290-340             AC163703.4         168067-168117       c
            341-473             AC163703.4         167845-167977       c
            474-541             AC163703.4         167659-167726       c
            542-604             AC163703.4         167251-167313       c
            605-685             AC163703.4         166760-166840       c
            686-846             AC163703.4         166519-166679       c
            847-987             AC163703.4         166285-166425       c
            988-1189            AC163703.4         165364-165565       c
            1190-1300           AC163703.4         164236-164346       c
            1301-1388           AC163703.4         164050-164137       c
            1389-1458           AC163703.4         163853-163922       c
            1459-1596           AC163703.4         163164-163301       c
            1597-1917           AC163703.4         162777-163097       c
            1918-2000           AC163703.4         160064-160146       c
            2001-2104           AC163703.4         159877-159980       c
            2105-2215           AC163703.4         158687-158797       c
            2216-2287           AC163703.4         158543-158614       c
            2288-2772           AC163703.4         157952-158436       c
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.44 cM"
     gene            1..2772
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /note="hook microtubule tethering protein 2"
                     /db_xref="GeneID:170833"
                     /db_xref="MGI:MGI:2181664"
     exon            1..130
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     CDS             86..2434
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /note="isoform 5 is encoded by transcript variant 6;
                     protein Hook homolog 2"
                     /codon_start=1
                     /product="protein Hook homolog 2 isoform 5"
                     /protein_id="NP_001359286.1"
                     /db_xref="GeneID:170833"
                     /db_xref="MGI:MGI:2181664"
                     /translation="MSVDKAELCGSLLTWLQTFQVSPPCASPQDLSSGLAIAHVLNQI
                     DPSWFNNEWLQGISEDSSPSWRLKVRKLEKILQSLVEYSKNVLGHPVSDQHLPDVSLI
                     GEFSNPAELGKLLQLVLGCAISCEKKQEYIQRIMTLEESVQHVVMEAIQELMTKDTPD
                     SLSPENYGNFDTQSRRYYFLSEEVEEGDHLQQHYLDLERQLLLLSEEKQNLAQENAAL
                     RERVGRSEVESAPGLTAKKLLLLQSQLEQLQEENFRLESSREDDRLRCLELEREVAEL
                     QQRNQALTSLSQEAQALKDEMDELRQSSERARQLEATLNSCRRRLGELQELRRQVRQL
                     EERNAGHAERTRQLEEELRRAGSLRAQLEAQRRQVQELQGQWQEEAMKAEKWLFECRN
                     LEEKCDLVTKEKERLLTERDSLREANEELRCAQLQPRGLAQADLSLDPTPSGLENLAA
                     EILPAELRETLVRLQLENKRLCQQEAADRERQEELQRHLEEANRARHGLEAQQRLNQQ
                     QLSELRAQVEELQKALQEQGGKTEDVSACLLPAWPSLVLPSNPPFSLPPCCPMPEEGH
                     CEYHGSREAHHCYLHSFSDLVPVPSLPAASLPQPTLLKRKLEDHLQKLHEADLELQRK
                     REYIEELEPPTDSSTARRIEELQDSLQKKDADLRAMEERYRRYVDKARTVIQTLEPKQ
                     RPPTVVSPEFHTLRSQLWERNLRIRQMEMDYEKSRRRQEQEEKLLISAWYSMGMALEH
                     RAGEEHAPAHAQSFLAQQRLATNARRGPLGRQALSLRPTDKH"
     exon            131..216
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            217..289
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            290..340
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            341..473
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            474..541
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            542..604
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            605..685
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            686..846
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            847..987
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1189
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1190..1300
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1388
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1458
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1596
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1917
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2000
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2001..2104
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2215
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2216..2287
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2772
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2754..2759
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /note="hexamer: AATAAA"
     polyA_site      2772
                     /gene="Hook2"
                     /gene_synonym="A630054I03Rik; mHK2"
                     /note="major polyA site"
ORIGIN      
        1 gcttcgggcg ctcgctggtg gcggacccgg aggctgctgc tgcgtgccgg ggctccttgg
       61 cccggatcga attcgatccg gagccatgag tgtggacaag gccgagctat gcgggtctct
      121 gctcacctgg ttgcagacgt tccaagtttc acccccctgt gccagtcccc aggacctgag
      181 cagcggactc gccatagccc atgtgctgaa tcagatagac ccttcctggt tcaacaacga
      241 atggctccag ggcatctcag aggactcaag ccccagctgg aggttgaagg tcaggaaact
      301 ggagaagatc ttacagagct tggtggaata ctcgaagaat gttctggggc accctgtgtc
      361 agatcagcat ctcccagatg tgagcctcat tggcgagttc tcaaatcctg cagaacttgg
      421 caaactgctt cagttggtac tgggctgtgc tatcagttgt gagaaaaaac aggagtatat
      481 ccaaagaatt atgaccctgg aggagtctgt tcaacatgta gtgatggaag ccatccagga
      541 gctcatgacc aaagacactc ctgattccct gtcaccagag aattacggga attttgatac
      601 acagtcccgc aggtactatt tcctgagtga ggaggtggag gagggtgatc atcttcagca
      661 acattacctg gatctggagc gccagctctt gctcctttca gaggagaagc agaatctggc
      721 acaagagaac gctgcccttc gagagagggt gggccggtcg gaagtggaga gtgccccggg
      781 cctcacagct aagaagctat tgcttctgca gtcacagcta gaacagcttc aagaggagaa
      841 tttcaggctg gagagtagcc gggaagatga tcggcttcgc tgcctggaac tagagcgcga
      901 ggtcgctgag ctgcaacaac ggaaccaggc actgaccagc ctatcccagg aggcacaagc
      961 actaaaggat gagatggatg agctgcggca gtcctccgaa cgtgcaaggc agctggaggc
     1021 cactctgaac agctgccgtc gtcgcctggg cgagctccaa gagctccggc ggcaggtgcg
     1081 gcagctggag gagcgaaatg caggccacgc ggagcgcacg cggcagctgg aagaggagct
     1141 gcgccgtgca ggatccctgc gtgcccagct cgaggctcag cgcaggcagg tgcaagagtt
     1201 gcagggccag tggcaggaag aggccatgaa ggctgagaag tggctgtttg aatgccgaaa
     1261 cctagaggaa aagtgtgacc tggtgacaaa ggagaaggag cggctattga ctgagaggga
     1321 ctccctgagg gaggccaatg aggagctccg ctgtgcccag ctgcagccca ggggattggc
     1381 tcaggctgat ctctccctgg atcctacccc ttctggcctg gaaaacttag cagcagagat
     1441 cctgcctgca gagctcagag agacgctagt gcgacttcag ctggagaaca agagactgtg
     1501 ccagcaggag gccgcggacc gggagcgaca ggaggaactg cagcgccacc tggaggaggc
     1561 caaccgtgca cgccatgggc tggaagcaca gcaacggctg aaccagcagc agctgtcgga
     1621 gctgagggcg caggtggaag agctacagaa ggccttgcag gagcaggggg gcaagaccga
     1681 ggacgtgagt gcctgcctcc tccctgcttg gccctccctt gtgctcccca gtaatccccc
     1741 tttctccctc cccccttgct gcccgatgcc tgaagaaggc cactgtgagt accacgggtc
     1801 cagggaggcg caccactgtt acctgcacag cttctcagac cttgtccccg tcccctcact
     1861 ccccgctgct tctcttcccc agcccaccct gctgaagagg aagctggagg atcatctgca
     1921 gaagctgcat gaggctgacc tggagttgca gaggaaacga gaatacattg aggagttgga
     1981 gcctcccact gacagcagca cagctcggcg gatcgaggag ctacaggaca gtctgcagaa
     2041 gaaggatgca gacctgcggg ccatggagga acgataccgc cgctacgtgg acaaggcgcg
     2101 cacggtcatt cagaccctgg aacccaagca gaggccaccc actgtggtgt ccccagaatt
     2161 ccataccttg aggtcacagc tctgggagcg gaacctgcgg atacggcaga tggagatgga
     2221 ctatgagaag agtcggagac ggcaggaaca ggaggaaaag ctgcttatca gtgcctggta
     2281 cagcatgggc atggccttgg agcatcgggc tggggaagag cacgctcctg cccatgccca
     2341 gtccttcttg gcacagcaaa gactggccac caatgctcgc cgtggacccc tggggcgcca
     2401 ggctctgagc ctccgcccca ctgacaagca ctgacaagac ctcgacttcc tgctgcctgc
     2461 tggagtcttc cagtttacat ttacccagct gggcctttga ccttgattga gcctgacttc
     2521 tgccctttgc ttccttagat gggagagaag gggacaggca ggtagcaggt gtggaactat
     2581 tccttttagc aatgtgattc ttcttgattc ttctcctggg cctcgtgtac tacctgggaa
     2641 gagcctgatt ttatatttga aaagaataaa gactttaaat ctgtcatgaa tgtactctag
     2701 acacaatgac gaagaataaa aactgcactg gggacacaga ccattgcttt taaaataaag
     2761 tttttacatg ta
//
