LOCUS       NM_008254               1469 bp    mRNA    linear   ROD 26-MAR-2023
DEFINITION  Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A lyase (Hmgcl),
            transcript variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_008254
VERSION     NM_008254.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Zhong C, Xiong G, Yang H, Du X, Du J, Yao F, Fang W and Deng Y.
  TITLE     Phosphorylation by IKKbeta Promotes the Degradation of HMGCL via
            NEDD4 in Lung Cancer
  JOURNAL   Int J Biol Sci 19 (4), 1110-1122 (2023)
   PUBMED   36923932
  REMARK    GeneRIF: Phosphorylation by IKKbeta Promotes the Degradation of
            HMGCL via NEDD4 in Lung Cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Wagner GR, Bhatt DP, O'Connell TM, Thompson JW, Dubois LG, Backos
            DS, Yang H, Mitchell GA, Ilkayeva OR, Stevens RD, Grimsrud PA and
            Hirschey MD.
  TITLE     A Class of Reactive Acyl-CoA Species Reveals the Non-enzymatic
            Origins of Protein Acylation
  JOURNAL   Cell Metab 25 (4), 823-837 (2017)
   PUBMED   28380375
REFERENCE   3  (bases 1 to 1469)
  AUTHORS   Wu Y, Wei FY, Kawarada L, Suzuki T, Araki K, Komohara Y, Fujimura
            A, Kaitsuka T, Takeya M, Oike Y, Suzuki T and Tomizawa K.
  TITLE     Mtu1-Mediated Thiouridine Formation of Mitochondrial tRNAs Is
            Required for Mitochondrial Translation and Is Involved in
            Reversible Infantile Liver Injury
  JOURNAL   PLoS Genet 12 (9), e1006355 (2016)
   PUBMED   27689697
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1469)
  AUTHORS   Martin J, Balmer ML, Rajendran S, Maurhofer O, Dufour JF and
            St-Pierre MV.
  TITLE     Nutritional stress exacerbates hepatic steatosis induced by
            deletion of the histidine nucleotide-binding (Hint2) mitochondrial
            protein
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 310 (7), G497-G509 (2016)
   PUBMED   26767982
REFERENCE   5  (bases 1 to 1469)
  AUTHORS   Gauthier N, Wu JW, Wang SP, Allard P, Mamer OA, Sweetman L, Moser
            AB, Kratz L, Alvarez F, Robitaille Y, Lepine F and Mitchell GA.
  TITLE     A liver-specific defect of Acyl-CoA degradation produces
            hyperammonemia, hypoglycemia and a distinct hepatic Acyl-CoA
            pattern
  JOURNAL   PLoS One 8 (7), e60581 (2013)
   PUBMED   23861731
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1469)
  AUTHORS   Cox KB, Johnson KR and Wood PA.
  TITLE     Chromosomal locations of the mouse fatty acid oxidation genes
            Cpt1a, Cpt1b, Cpt2, Acadvl, and metabolically related Crat gene
  JOURNAL   Mamm Genome 9 (8), 608-610 (1998)
   PUBMED   9680378
REFERENCE   7  (bases 1 to 1469)
  AUTHORS   Baltscheffsky M, Brosche M, Hultman T, Lundvik L, Nyren P,
            Sakai-Nore Y, Severin A and Strid A.
  TITLE     A 3-hydroxy-3-methylglutaryl-CoA lyase gene in the photosynthetic
            bacterium Rhodospirillum rubrum
  JOURNAL   Biochim Biophys Acta 1337 (1), 113-122 (1997)
   PUBMED   9003443
REFERENCE   8  (bases 1 to 1469)
  AUTHORS   Wang SP, Robert MF, Gibson KM, Wanders RJ and Mitchell GA.
  TITLE     3-Hydroxy-3-methylglutaryl CoA lyase (HL): mouse and human HL gene
            (HMGCL) cloning and detection of large gene deletions in two
            unrelated HL-deficient patients
  JOURNAL   Genomics 33 (1), 99-104 (1996)
   PUBMED   8617516
REFERENCE   9  (bases 1 to 1469)
  AUTHORS   Kaushik N and Stoye JP.
  TITLE     Intracisternal A-type particle elements as genetic markers:
            detection by repeat element viral element amplified locus-PCR
  JOURNAL   Mamm Genome 5 (11), 688-695 (1994)
   PUBMED   7873878
REFERENCE   10 (bases 1 to 1469)
  AUTHORS   Wang S, Nadeau JH, Duncan A, Robert MF, Fontaine G, Schappert K,
            Johnson KR, Zietkiewicz E, Hruz P, Miziorko H et al.
  TITLE     3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL): cloning and
            characterization of a mouse liver HL cDNA and subchromosomal
            mapping of the human and mouse HL genes
  JOURNAL   Mamm Genome 4 (7), 382-387 (1993)
   PUBMED   8102917
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL672076.10, BB852743.1 and AK132035.1.
            
            [WARNING] On May 10, 2023 this sequence was replaced by
            NM_008254.4.
            
            On Nov 4, 2017 this sequence version replaced NM_008254.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132035.1, AK154033.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5                 AL672076.10        125559-125563
            6-17                BB852743.1         1-12
            18-1414             AK132035.1         2-1398
            1415-1469           AK132035.1         1399-1453
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 68.14 cM"
     gene            1..1469
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /note="3-hydroxy-3-methylglutaryl-Coenzyme A lyase"
                     /db_xref="GeneID:15356"
                     /db_xref="MGI:MGI:96158"
     exon            1..93
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     CDS             34..1011
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /EC_number="4.1.3.4"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; hydroxymethylglutaryl-CoA lyase, mitochondrial;
                     HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase"
                     /codon_start=1
                     /product="hydroxymethylglutaryl-CoA lyase, mitochondrial
                     isoform 1 precursor"
                     /protein_id="NP_032280.2"
                     /db_xref="CCDS:CCDS18795.1"
                     /db_xref="GeneID:15356"
                     /db_xref="MGI:MGI:96158"
                     /translation="MASVRKAFPRRLVGLTSLRAVSTSSMGTLPKQVKIVEVGPRDGL
                     QNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADHSDVLKGIQKFPGIN
                     YPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQRFAGVMQAAQAA
                     SISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGTPGLMKDML
                     TAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNL
                     ATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL"
     exon            94..177
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            178..285
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            286..381
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            382..530
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            531..594
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            595..783
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            784..909
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1469
                     /gene="Hmgcl"
                     /gene_synonym="HL"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggtgccca gtgggttggc ctccggagtg aagatggcgt cagtgaggaa ggctttcccg
       61 cggaggctgg tgggcttgac gtccctccgg gctgtgagca cctcctccat gggcactttg
      121 ccaaagcagg tgaagatcgt ggaagtcggt ccccgagatg ggctgcagaa tgaaaagagt
      181 attgtaccca ccccagtgaa gatcaggctg atcgacatgc tttccgaagc agggctcccc
      241 gtgatcgagg ccaccagctt tgtttctccc aagtgggtgc cacagatggc tgaccactct
      301 gacgtcttga agggcattca gaagtttccc ggcatcaact acccagtcct gactccaaac
      361 atgaaaggct ttgaggaagc ggtagctgcg ggtgccaagg aagtgagcgt cttcggtgct
      421 gtgtctgagc tcttcacccg gaagaacgcg aactgctcta tagaggagag tttccagcgc
      481 tttgctggag tcatgcaggc ggcccaggcc gccagcatct ctgtgagagg gtatgtctcc
      541 tgtgccctcg gatgccccta cgaggggaag gtctccccgg ctaaagttgc tgaggttgcc
      601 aagaagttgt actcgatggg ctgctatgag atctcccttg gggacaccat cggcgtaggt
      661 accccaggac tcatgaaaga catgctgact gctgtcatgc atgaagtgcc tgtgacagca
      721 ttggctgtcc actgccatga tacctatggt caagctctgg ccaacacctt ggtggccctg
      781 cagatgggtg tgagtgttgt ggactcctct gtggcaggac ttggaggctg tccctatgca
      841 aaaggggcat cagggaactt ggctactgag gacctggtct acatgctgaa tggcttaggg
      901 attcacacgg gtgtgaacct ccagaaactt ctagaagctg gggacttcat ctgtcaagcc
      961 cttaacagaa aaactagttc caaagtggca caggccacct gcaagctctg agaccctcgt
     1021 tcgcctgaac cagaacggag ggaattgggt gtatacaatg attcctggat gaagagatgg
     1081 aatgagagca aatgagccgg catcacagag gtccctgtcc ctgatgataa gggctagagc
     1141 tgcctggcct gggccagctc cccagagctg tgcctaagca cttgcttggg atggccctgg
     1201 gtgagtctgc ctgccaacag agctgatgtc caccgccgca gggcctttgt tctacctctg
     1261 aggacagaga gcagtttccc cttatgccag cagagcattt gctggaatgg tgggggttga
     1321 tctgcatctg tggtcatctg ccaacgaaat ctccactctg tgtgtgattt ttttgaaaac
     1381 agcttatgta attaaaggtt taattttcta atataacatt caaacgtgtg tttatgggtg
     1441 gaggagtaaa attgctctga gggaagagg
//
