LOCUS       NM_001033245            3219 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus hexokinase 3 (Hk3), transcript variant 1, mRNA.
ACCESSION   NM_001033245
VERSION     NM_001033245.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Zhu,M., Tang,X., Xu,J. and Gong,Y.
  TITLE     Identification of HK3 as a promising immunomodulatory and
            prognostic target in sepsis-induced acute lung injury
  JOURNAL   Biochem Biophys Res Commun 706, 149759 (2024)
   PUBMED   38484574
  REMARK    GeneRIF: Identification of HK3 as a promising immunomodulatory and
            prognostic target in sepsis-induced acute lung injury.
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3219)
  AUTHORS   van der Kooij,M.A., Rojas-Charry,L., Givehchi,M., Wolf,C.,
            Bueno,D., Arndt,S., Tenzer,S., Mattioni,L., Treccani,G., Hasch,A.,
            Schmeisser,M.J., Vianello,C., Giacomello,M. and Methner,A.
  TITLE     Chronic social stress disrupts the intracellular redistribution of
            brain hexokinase 3 induced by shifts in peripheral glucose levels
  JOURNAL   J Mol Med (Berl) 100 (10), 1441-1453 (2022)
   PUBMED   35943566
  REMARK    GeneRIF: Chronic social stress disrupts the intracellular
            redistribution of brain hexokinase 3 induced by shifts in
            peripheral glucose levels.
REFERENCE   4  (bases 1 to 3219)
  AUTHORS   Bulusu,V., Prior,N., Snaebjornsson,M.T., Kuehne,A., Sonnen,K.F.,
            Kress,J., Stein,F., Schultz,C., Sauer,U. and Aulehla,A.
  TITLE     Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to
            Mouse Embryo Mesoderm Development
  JOURNAL   Dev Cell 40 (4), 331-341 (2017)
   PUBMED   28245920
REFERENCE   5  (bases 1 to 3219)
  AUTHORS   Bowman,C.E., Zhao,L., Hartung,T. and Wolfgang,M.J.
  TITLE     Requirement for the Mitochondrial Pyruvate Carrier in Mammalian
            Development Revealed by a Hypomorphic Allelic Series
  JOURNAL   Mol Cell Biol 36 (15), 2089-2104 (2016)
   PUBMED   27215380
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3219)
  AUTHORS   Yavari,A., Stocker,C.J., Ghaffari,S., Wargent,E.T., Steeples,V.,
            Czibik,G., Pinter,K., Bellahcene,M., Woods,A., Martinez de
            Morentin,P.B., Cansell,C., Lam,B.Y., Chuster,A., Petkevicius,K.,
            Nguyen-Tu,M.S., Martinez-Sanchez,A., Pullen,T.J., Oliver,P.L.,
            Stockenhuber,A., Nguyen,C., Lazdam,M., O'Dowd,J.F., Harikumar,P.,
            Toth,M., Beall,C., Kyriakou,T., Parnis,J., Sarma,D., Katritsis,G.,
            Wortmann,D.D., Harper,A.R., Brown,L.A., Willows,R., Gandra,S.,
            Poncio,V., de Oliveira Figueiredo,M.J., Qi,N.R., Peirson,S.N.,
            McCrimmon,R.J., Gereben,B., Tretter,L., Fekete,C., Redwood,C.,
            Yeo,G.S., Heisler,L.K., Rutter,G.A., Smith,M.A., Withers,D.J.,
            Carling,D., Sternick,E.B., Arch,J.R., Cawthorne,M.A., Watkins,H.
            and Ashrafian,H.
  TITLE     Chronic Activation of gamma2 AMPK Induces Obesity and Reduces beta
            Cell Function
  JOURNAL   Cell Metab 23 (5), 821-836 (2016)
   PUBMED   27133129
REFERENCE   7  (bases 1 to 3219)
  AUTHORS   Lundgaard,I., Li,B., Xie,L., Kang,H., Sanggaard,S., Haswell,J.D.,
            Sun,W., Goldman,S., Blekot,S., Nielsen,M., Takano,T., Deane,R. and
            Nedergaard,M.
  TITLE     Direct neuronal glucose uptake heralds activity-dependent increases
            in cerebral metabolism
  JOURNAL   Nat Commun 6, 6807 (2015)
   PUBMED   25904018
  REMARK    GeneRIF: Hexokinase expression is highly enriched in neurons
            compared to astrocytes.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 3219)
  AUTHORS   Leite,T.C., Coelho,R.G., Da Silva,D., Coelho,W.S.,
            Marinho-Carvalho,M.M. and Sola-Penna,M.
  TITLE     Lactate downregulates the glycolytic enzymes hexokinase and
            phosphofructokinase in diverse tissues from mice
  JOURNAL   FEBS Lett 585 (1), 92-98 (2011)
   PUBMED   21074528
REFERENCE   9  (bases 1 to 3219)
  AUTHORS   Cankaya,M., Hernandez,A.M., Ciftci,M., Beydemir,S., Ozdemir,H.,
            Budak,H., Gulcin,I., Comakli,V., Emircupani,T., Ekinci,D., Kuzu,M.,
            Jiang,Q., Eichele,G. and Kufrevioglu,O.I.
  TITLE     An analysis of expression patterns of genes encoding proteins with
            catalytic activities
  JOURNAL   BMC Genomics 8, 232 (2007)
   PUBMED   17626619
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154610.3, AK171580.1 and BI133441.1.
            
            On Apr 22, 2011 this sequence version replaced NM_001033245.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1 and 2 encode the same isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154603.1, AK171580.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AC154610.3         91403-91453
            52-3216             AK171580.1         2-3166
            3217-3219           BI133441.1         18-20               c
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 29.8 cM"
     gene            1..3219
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /note="hexokinase 3"
                     /db_xref="GeneID:212032"
                     /db_xref="MGI:MGI:2670962"
     exon            1..240
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            241..360
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..3033
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /EC_number="2.7.1.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     hexokinase type III; hexokinase-C"
                     /codon_start=1
                     /product="hexokinase-3 isoform 1"
                     /protein_id="NP_001028417.1"
                     /db_xref="CCDS:CCDS26538.1"
                     /db_xref="GeneID:212032"
                     /db_xref="MGI:MGI:2670962"
                     /translation="MATIGPSGLHPGERASVCPHEGVPRPSGSLELECLQQFKVTRTQ
                     LQQIQASLLCSMEQALKGQDSPAPSVRMLPTYVRSTPHGTEQGDFLVLELGATGASLR
                     VLWVTLTGTKECRVEPRSREFVIPQEVILGAGQQLFDFAARCLSEFLDAYPVENQGLK
                     LGFNFSFPCHQTGLDRSTLISWTKGFRCSGVEGQDVVQLLRDAIQRQGTYRIDVVAMV
                     NDTVGTMMGCELGTRPCEVGLIVDTGTNACYMEEARHVAALDEDRGRTCVSIEWGSFY
                     DEDALGPVLTTFDSALDRESLTPGAQRFEKMIGGLYLGELVRLVLVHLTQHGVLFDGC
                     ASPALLSQGCILLDHVAEMEDTATGTARVHTILQDLGLSPRASDAELVQYVCVAVCTR
                     AAQLCAAALAAVLSRLQHSREQQTLQVAVATGGRVFERHPRFLRILKETVTLLAPNCD
                     VSFIPSVDGGGRGVAMVTAVAARLAAHRRILEETLAPFQLTLEQMTVVQAQMREAMIR
                     GLQGEASSLRMLPTYVRATPDGSERGDFLALDLGGTNFRVLLVRVAEGSVQIINQVYS
                     IPECRAQGSGQKLFDHIVDCIVDFQKRQGLSGQSLPLGFTFSFPCKQLGLDQGILLNW
                     TKGFNASGCEGQDVVYLLREAIRRRQAVELNVVAIVNDTVGTMMSCGYDDPRCEMGLI
                     VGTGTNACYMEELRNVASVPGDSGLMCINMEWGAFGDDGSLGTLSTRFDTSVDQASIN
                     PGKQRFEKMISGMYLGEIVRHILLHLTNLGVLFRGQKTQCLQARDIFKTKFLSEIESD
                     SLALRQVRAILEDLGLTLTSDDALMVLEVCQAVSRRAAQLCGAGVAAVVEKIRENRGL
                     QELTVSVGVDGTLYKLHPHFSKLVSATVRKLAPQCTVTFLQSEDGSGKGAALVTAVAC
                     RLTQMAHV"
     exon            361..517
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            518..672
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            673..792
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            793..888
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            889..988
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1172
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1328
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1562
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            1563..1858
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1998
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            1999..2118
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2214
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2314
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2498
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            2499..2654
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2888
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     exon            2889..3219
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3191..3196
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /note="hexamer: AATATA"
     polyA_site      3219
                     /gene="Hk3"
                     /gene_synonym="HK III; HK-III"
                     /note="major polyA site"
ORIGIN      
        1 gtggtcatct ggcttgtttg taccatggcc ctgcccattc tgggctccgt tgagagcaga
       61 tttgtaggga agtgcccttt cctgaattag cagatttcgg ttaagtggct acagaggaag
      121 gtggagcttg gggagctgac ccagagtttt cacttttgag tttagtccag tatcactgtg
      181 gtcatatttg ggagaagagc tgagcccccg aggaaggtga tcaacttctg aatctcccag
      241 gtagtcaata cccattgtgg aaacatggcc accattgggc cttctggcct gcatcctgga
      301 gaaagagcct cagtctgccc acacgagggc gtgccaaggc cctcaggtag cttagaactg
      361 gaatgcttgc agcaattcaa ggtgaccagg acacagttgc agcagatcca agccagtctc
      421 ctgtgctcca tggagcaggc actgaaggga caggacagtc ccgctccttc tgtccggatg
      481 ctgcccacat acgtgaggtc cacaccacac ggcaccgagc aaggggattt cctggtgctg
      541 gagctggggg ccacaggagc atcactacgt gtgttgtggg taacactgac aggcaccaaa
      601 gaatgccggg tggagcccag gagccgggag tttgtgattc ctcaagaggt gatcctaggt
      661 gctggccagc agctctttga ctttgctgcc cgatgcctct ctgaattcct ggatgcatac
      721 cccgtggaga atcagggtct gaagcttggg ttcaatttct cttttccttg tcaccagaca
      781 ggcttggaca ggagcaccct catttcctgg acaaaaggtt ttaggtgcag cggcgtggaa
      841 ggccaggatg tggtccagtt gctaagagat gccattcaga ggcaggggac ctacaggatt
      901 gatgtggtag ccatggtgaa tgacacggtg ggcaccatga tgggctgtga gctgggcacc
      961 aggccgtgtg aagtcgggct gattgtagac accggtacca acgcgtgcta catggaggag
     1021 gcgcggcacg tggccgctct ggatgaggac cgcggccgca cctgtgtcag catcgagtgg
     1081 ggctccttct atgacgaaga tgctctaggg ccagtactga ccaccttcga ctctgccctg
     1141 gaccgcgagt ccctgactcc tggtgctcaa aggtttgaga agatgattgg aggcctttac
     1201 ctgggtgagc tggtgaggct ggttctggtc cacttgaccc agcacggggt cctctttgat
     1261 ggctgcgcct ctcctgcgtt gctgagtcaa ggctgtatcc tcctggacca cgtggctgag
     1321 atggaggaca ccgccactgg gacagcccgc gtccacacca tcctgcagga cttgggtctg
     1381 agccctcggg cctcagatgc tgaactggtg caatatgtgt gcgtggctgt gtgcacacgg
     1441 gctgcccagc tctgtgcggc tgccctggct gcagtcctct cccgcctcca gcacagcagg
     1501 gagcagcaga cattgcaagt ggctgtggcc actggagggc gagtgtttga acgacacccc
     1561 aggttcctcc gaatcctaaa ggagacggta acactcctgg ccccaaactg tgatgtctcc
     1621 ttcatcccct ctgtggatgg tggtggccgg ggtgtggcaa tggtgacagc tgtggctgcc
     1681 cgcctggctg ctcacaggcg catcctggaa gagaccctgg caccatttca gctgaccttg
     1741 gagcagatga cagtggtgca ggcacaaatg cgggaagcca tgatcagggg gcttcaagga
     1801 gaggcctcct ccctccgcat gctgcccact tacgtccgag cgacgcccga cggcagcgag
     1861 cgaggtgatt tcctggcctt ggacctaggg ggcaccaact tccgggtcct gttggtacga
     1921 gtggcggagg gcagtgtgca gatcatcaac caggtctact ccattcctga gtgtagagcc
     1981 cagggctctg gacagaagct ctttgaccat attgtggact gcatcgtgga cttccagaag
     2041 aggcagggcc ttagtggaca gagcctaccc ctgggtttca ccttctcttt cccatgcaag
     2101 cagcttggcc tggaccaggg catcctcctg aattggacta aggggttcaa tgcatcaggc
     2161 tgtgagggcc aggatgttgt gtatctatta cgggaagcca ttaggcgcag acaggcagtg
     2221 gagctgaatg tggttgccat tgtcaatgac acggtgggga ccatgatgtc ctgtggctat
     2281 gatgatcccc gttgtgagat gggcctcatt gtcggaaccg gcaccaacgc ctgttacatg
     2341 gaagagctcc gcaatgtggc aagtgtgcct ggggactcgg gcctcatgtg catcaacatg
     2401 gagtggggtg cctttgggga tgatggctca ctgggcacac tcagcacccg ctttgacacc
     2461 agtgtggacc aggcatccat caatccaggc aaacagaggt ttgagaaaat gatcagcggt
     2521 atgtacttgg gggagatcgt ccgccatatc ctcctgcact taaccaatct cggagttctc
     2581 ttccgaggcc agaagactca atgccttcag gccagggaca tcttcaagac taagttcctc
     2641 tctgagatcg aaagtgatag cctggccctg cgtcaggtcc gagccatcct ggaagatctg
     2701 gggctgactc tgacatctga tgacgccttg atggtcctgg aagtgtgcca ggctgtgtct
     2761 cgcagggctg cccaactctg tggggcaggt gtggctgccg tggtggagaa gattcgggag
     2821 aaccggggcc tgcaggagct gacagtgtct gtgggagtag atgggacact ctacaagcta
     2881 catcctcact tctccaagct ggtgtcggca acagtccgga agctagcccc tcagtgcacc
     2941 gtcacctttt tgcagtcgga ggatgggtct ggcaaaggcg cagctttggt tactgctgtt
     3001 gcttgtcgcc ttacccagat ggcccacgtc tgaggaaatc tccaggaacg aagcaatctc
     3061 attggactga ggcttcagcc taccctgatc ctcagccacc cacaactccc aaagaaaccc
     3121 gtgtgtggtc cccttatagc cgtctgccca cacctaatgg ctttgcaaga gaagcgacac
     3181 tccggttagc aatatatata tataatttat ttacattca
//
