LOCUS       NM_001146100            4157 bp    mRNA    linear   ROD 05-FEB-2024
DEFINITION  Mus musculus hexokinase 1 (Hk1), transcript variant 1, mRNA.
ACCESSION   NM_001146100
VERSION     NM_001146100.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Martinez-Garza,U., Choi,J., Scafidi,S. and Wolfgang,M.J.
  TITLE     Proteomics identifies the developmental regulation of HKDC1 in
            liver of pigs and mice
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 325 (4), R389-R400 (2023)
   PUBMED   37545422
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Chong,D., Gu,Y., Zhang,T., Xu,Y., Bu,D., Chen,Z., Xu,N., Li,L.,
            Zhu,X., Wang,H., Li,Y., Zheng,F., Wang,D., Li,P., Xu,L., Hu,Z. and
            Li,C.
  TITLE     Neonatal ketone body elevation regulates postnatal heart
            development by promoting cardiomyocyte mitochondrial maturation and
            metabolic reprogramming
  JOURNAL   Cell Discov 8 (1), 106 (2022)
   PUBMED   36220812
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4157)
  AUTHORS   De Jesus,A., Keyhani-Nejad,F., Pusec,C.M., Goodman,L., Geier,J.A.,
            Stoolman,J.S., Stanczyk,P.J., Nguyen,T., Xu,K., Suresh,K.V.,
            Chen,Y., Rodriguez,A.E., Shapiro,J.S., Chang,H.C., Chen,C.,
            Shah,K.P., Ben-Sahra,I., Layden,B.T., Chandel,N.S., Weinberg,S.E.
            and Ardehali,H.
  TITLE     Hexokinase 1 cellular localization regulates the metabolic fate of
            glucose
  JOURNAL   Mol Cell 82 (7), 1261-1277 (2022)
   PUBMED   35305311
  REMARK    GeneRIF: Hexokinase 1 cellular localization regulates the metabolic
            fate of glucose.
REFERENCE   4  (bases 1 to 4157)
  AUTHORS   Sofer,S., Lamkiewicz,K., Armoza Eilat,S., Partouche,S., Marz,M.,
            Moskovits,N., Stemmer,S.M., Shlomai,A. and Sklan,E.H.
  TITLE     A genome-wide CRISPR activation screen reveals Hexokinase 1 as a
            critical factor in promoting resistance to multi-kinase inhibitors
            in hepatocellular carcinoma cells
  JOURNAL   FASEB J 36 (3), e22191 (2022)
   PUBMED   35147243
  REMARK    GeneRIF: A genome-wide CRISPR activation screen reveals Hexokinase
            1 as a critical factor in promoting resistance to multi-kinase
            inhibitors in hepatocellular carcinoma cells.
REFERENCE   5  (bases 1 to 4157)
  AUTHORS   Nacarino-Palma,A., Gonzalez-Rico,F.J., Rejano-Gordillo,C.M.,
            Ordiales-Talavero,A., Merino,J.M. and Fernandez-Salguero,P.M.
  TITLE     The aryl hydrocarbon receptor promotes differentiation during mouse
            preimplantational embryo development
  JOURNAL   Stem Cell Reports 16 (9), 2351-2363 (2021)
   PUBMED   34478649
REFERENCE   6  (bases 1 to 4157)
  AUTHORS   Griffin,L.D., Gelb,B.D., Wheeler,D.A., Davison,D., Adams,V. and
            McCabe,E.R.
  TITLE     Mammalian hexokinase 1: evolutionary conservation and structure to
            function analysis
  JOURNAL   Genomics 11 (4), 1014-1024 (1991)
   PUBMED   1783373
REFERENCE   7  (bases 1 to 4157)
  AUTHORS   Threadgill,D.S. and Womack,J.E.
  TITLE     Mapping HSA10 homologous loci in cattle
  JOURNAL   Cytogenet Cell Genet 57 (2-3), 123-126 (1991)
   PUBMED   1655359
REFERENCE   8  (bases 1 to 4157)
  AUTHORS   Kozak,C.A., Peyser,M., Krall,M., Mariano,T.M., Kumar,C.S.,
            Pestka,S. and Mock,B.A.
  TITLE     Molecular genetic markers spanning mouse chromosome 10
  JOURNAL   Genomics 8 (3), 519-524 (1990)
   PUBMED   1981053
REFERENCE   9  (bases 1 to 4157)
  AUTHORS   Lalley,P.A., Minna,J.D. and Francke,U.
  TITLE     Conservation of autosomal gene synteny groups in mouse and man
  JOURNAL   Nature 274 (5667), 160-163 (1978)
   PUBMED   662012
REFERENCE   10 (bases 1 to 4157)
  AUTHORS   Lalley,P.A., Francke,U. and Minna,J.D.
  TITLE     Assignment of the genes coding for pyrophosphatase and hexokinase-1
            to mouse chromosome 10: implications for comparative gene mapping
            in man and mouse
  JOURNAL   Cytogenet Cell Genet 22 (1-6), 570-572 (1978)
   PUBMED   752542
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC072628.1 and AK161466.1.
            
            [WARNING] On Mar 14, 2024 this sequence was replaced by
            NM_001146100.2.
            
            Transcript Variant: This variant (1) represents the ubiquitously
            expressed transcript and encodes isoform HK1. Its 5' end includes
            an exon that is unique to this transcript and encodes a distinct
            N-terminus containing the porin binding domain (PBD). The porin
            binding domain mediates association with the mitochondrial
            membrane.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC072628.1, SRR1660821.39663.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3908              BC072628.1         122-4029
            3909-4157           AK161466.1         2822-3070
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 32.37 cM"
     gene            1..4157
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /note="hexokinase 1"
                     /db_xref="GeneID:15275"
                     /db_xref="MGI:MGI:96103"
     exon            1..409
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     CDS             347..3103
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /EC_number="2.7.1.1"
                     /note="isoform HK1 is encoded by transcript variant 1; HK
                     I; hexokinase type I; hexokinase, tumor isozyme; downeast
                     anemia"
                     /codon_start=1
                     /product="hexokinase-1 isoform HK1"
                     /protein_id="NP_001139572.1"
                     /db_xref="CCDS:CCDS48577.1"
                     /db_xref="GeneID:15275"
                     /db_xref="MGI:MGI:96103"
                     /translation="MIAAQLLAYYFTELKDDQVKKIDKYLYAMRLSDEILIDILTRFK
                     KEMKNGLSRDYNPTASVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKS
                     QNVSMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCRQ
                     SKIDEAVLITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCG
                     YDDQQCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTE
                     FDRELDRGSLNPGKQLFEKMVSGMYMGELVRLILVKMAKESLLFEGRITPELLTRGKF
                     TTSDVAAIETDKEGVQNAKEILTRLGVEPSHDDCVSVQHVCTIVSFRSANLVAATLGA
                     ILNRLRDNKGTPRLRTTVGVDGSLYKMHPQYSRRFHKTLRRLVPDSDVRFLLSESGSG
                     KGAAMVTAVAYRLAEQHRQIEETLSHFRLSKQALMEVKKKLRSEMEMGLRKETNSRAT
                     VKMLPSYVRSIPDGTEHGDFLALDLGGTNFRVLLVKIRSGKKRTVEMHNKIYSIPLEI
                     MQGTGDELFDHIVSCISDFLDYMGIKGPRMPLGFTFSFPCKQTSLDCGILITWTKGFK
                     ATDCVGHDVATLLRDAVKRREEFDLDVVAVVNDTVGTMMTCAYEEPSCEIGLIVGTGS
                     NACYMEEMKNVEMVEGNQGQMCINMEWGAFGDNGCLDDIRTDFDKVVDEYSLNSGKQR
                     FEKMISGMYLGEIVRNILIDFTKKGFLFRGQISEPLKTRGIFETKFLSQIESDRLALL
                     QVRAILQQLGLNSTCDDSILVKTVCGVVSKRAAQLCGAGMAAVVEKIRENRGLDHLNV
                     TVGVDGTLYKLHPHFSRIMHQTVKELSPKCTVSFLLSEDGSGKGAALITAVGVRLRGD
                     PTNA"
     exon            410..572
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            573..721
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            722..841
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            842..937
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            938..1037
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1221
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1377
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1611
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1612..1916
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2065
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2185
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2186..2281
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2282..2381
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2382..2565
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2721
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2722..2955
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
     exon            2956..4157
                     /gene="Hk1"
                     /gene_synonym="dea; Hk-1; Hk1-s; mHk1-s"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gctccttacg tggggaacgt gcagggtgat gcctcctttc cggcgcggga gggagcggcc
       61 gtcattctcc tgctctggct gggaagcccc agccatagcg cggcagtggc gccttgtagc
      121 caatggggac tgaggaggtg ggccggctgg cggttgtcac cctcccgggg acgggagctc
      181 ggaggtctgg agagagcagg cagacgcccc gcccagggac tgagggggag gagccaaggg
      241 aggaggaggt ggagtcgcgg atccgccgct ggaggaccac tgctcaccag ggctactgag
      301 gagccactgg ccccacacct gcttctccgc atcccccacc gtcaacatga tcgccgcgca
      361 actactggca tattacttca ccgagctgaa ggatgaccaa gtcaaaaaga ttgataagta
      421 tctgtatgcc atgcggctct ctgatgaaat tctgatagat atcctgacac gcttcaagaa
      481 agagatgaag aatggcctct cccgggatta taacccaacg gcctccgtca agatgctgcc
      541 aacctttgtc cggtccattc cggacggctc agaaaagggg gatttcattg cactggatct
      601 cggcgggtct tcctttcgaa tcctgcgggt gcaggtgaac cacgagaaga gtcagaacgt
      661 cagcatggag tctgaggtct acgacacccc agagaacatc gtgcacggca gtggaagcca
      721 gctttttgat cacgtcgctg aatgcctcgg agacttcatg gagaaaagga agatcaagga
      781 caagaaatta cccgtgggat tcacgttttc cttcccgtgc cgacaatcca aaatagacga
      841 ggccgtactg atcacgtgga caaagcggtt caaagccagt ggcgtggaag gggcggatgt
      901 ggtcaagctg ctgaataaag ccattaagaa gcgaggggac tatgacgcta acattgtagc
      961 tgtggtgaat gacacagtgg ggaccatgat gacctgcggc tacgatgacc aacagtgtga
     1021 agtcggcctg atcattggca ctggcaccaa tgcttgctac atggaggaac tgcgacacat
     1081 cgacctggtg gaaggcgatg aggggaggat gtgtattaac acggaatggg gagcctttgg
     1141 ggatgatggg tccctggaag acattcgaac agagtttgac agagagttag accggggatc
     1201 cctcaaccct gggaaacagc tgttcgagaa gatggtgagc ggcatgtaca tgggggagct
     1261 ggtccggctg atcctggtga agatggccaa ggaaagcctc ttatttgaag ggcgcattac
     1321 tccagagctg ctcacgaggg gcaagttcac cactagcgac gtagccgcca ttgaaacgga
     1381 taaggaaggc gttcaaaatg ccaaggaaat cttgacccgc ctgggagtgg agccgtctca
     1441 cgatgactgc gtatcggtcc agcacgtatg cacgatcgtc tccttccgat cagccaacct
     1501 ggtggctgcc acgctcggtg ccatcttgaa ccgcctgcgg gacaataagg gcacgcccag
     1561 gctgcggacc acagttggcg tagacggttc tctctacaag atgcacccac agtattcccg
     1621 gcggttccac aagaccctga ggcgcctggt gcctgactcg gacgtccggt tcctcctctc
     1681 ggagagtggc agtggcaagg gagccgccat ggtgaccgct gtggcctacc gcctggccga
     1741 gcagcaccgg cagattgagg aaaccctgtc ccacttccgc ctcagcaagc aggcactgat
     1801 ggaggtgaag aagaagctgc ggtcagagat ggaaatgggg ctgagaaagg agaccaacag
     1861 cagagctacg gtcaaaatgc tgccttctta tgttcggagc atcccagatg ggactgagca
     1921 tggtgacttc ctggccttgg atctcggagg aacgaatttc cgagtcctac tggtaaagat
     1981 ccgtagtggg aaaaagagaa cagtggagat gcacaacaag atctactcca ttcccctgga
     2041 aatcatgcag ggcaccgggg atgagctgtt tgatcacatt gtctcctgca tctccgactt
     2101 cctggactac atggggatca aaggcccccg gatgcctctg ggcttcacct tctcgtttcc
     2161 ctgcaagcag acgagcctag attgcggaat cttgatcacg tggacaaagg gattcaaagc
     2221 caccgactgt gtgggtcacg atgtagccac tttactgagg gatgctgtaa aaaggagaga
     2281 ggaatttgac ctggatgtgg tggctgtggt caacgacacc gtgggcacca tgatgacttg
     2341 tgcttatgaa gaaccttctt gtgagattgg actcatcgtg gggactggca gcaatgcctg
     2401 ctacatggag gagatgaaaa acgtggagat ggtggagggg aaccagggcc agatgtgcat
     2461 caatatggaa tggggtgcct ttggtgacaa cggctgtctg gacgacatca gaacagactt
     2521 cgacaaagtg gtggacgaat attctctaaa cagtgggaaa caaaggtttg agaagatgat
     2581 cagtggaatg tacctgggtg agatcgtccg taacatcctg attgacttca ccaagaaagg
     2641 cttcctcttc cggggacaga tctctgagcc actcaagacc cgaggcatct tcgagaccaa
     2701 gtttctctct cagatcgaga gtgaccgatt agcgctgctc caggtgcggg ccatccttca
     2761 gcagctgggt ctaaacagca cgtgcgacga cagtatcctg gtcaagaccg tgtgtggggt
     2821 ggtgtccaag cgggcggccc agctgtgtgg tgccggcatg gccgccgtgg tggaaaagat
     2881 ccgagagaac agaggcctag accacctgaa tgtaaccgtg ggcgtggacg ggacgctcta
     2941 caaactccat ccacacttct ccagaatcat gcaccaaaca gtgaaggaac tgtcaccaaa
     3001 gtgtaccgtg tccttcctcc tgtctgaaga cggcagcggc aagggggccg cccttatcac
     3061 agctgtgggc gtgcggctca gaggagaccc tacgaacgcc taagagccag gatcctccca
     3121 gcccccagcc cgccaccctt ccagcactcc tctctagaac cgacgaccac acaccgcccc
     3181 cgtgttccac ccagcaagct ccgctgggag acccagcgcc ggcacccact ctgccgcaga
     3241 gggaggaagg ggactgcagt aacggagcat cacatagaat accacccgga gcgcgtgctg
     3301 ttgatgatct gatctctcgc ctggacccct catccctgcc ctgccacttt gcatgattca
     3361 agttcgacct ggccatgcat tgcccaagcg tgaacgtagt ggcacccacg tctaccgcag
     3421 gcgtccagcc aggaaagagc gacccccctc ttggactgtc ttctgggccc ttccaagccc
     3481 atctgtggag tcgcctccct ccttcccccc acccccgtgt gaagtgtgtt atcaccagca
     3541 gacactgccg gactcctgcc cacaggggcg tggcctgaag ggcgagtgtg aacatggcac
     3601 tgctgttccc ttccccctcc ctgcacccgc cgcagcctgc catcccattt ggatgtatca
     3661 atgccactga gttgtctgtc cgtccgtgga gccagtccta gccacattat tgacagtctt
     3721 gcattttgtt ttgtctcgtg atggtggtgg tggtaggggt acgcttaggt gggcagtcct
     3781 gtggggaaaa catcttgtct ccatcttgct aggaagaacc aacccacaaa acaacgccat
     3841 cactggaatc tcccatcgct ttagtgagcc attgtcgtat gtctagtaaa ctttgtactg
     3901 attcaaaagt cggagtggtg tttccttggg gtgggggcaa gggcttagca tatggtgaca
     3961 gccacgctag gggctttatg gactcctggg tggggagaaa gcttcaaagc tccccaggta
     4021 tgcctatagt cccagcactt gggaaatgga ggagtgagtt caagtccctc cttggctaca
     4081 tagtttcagg caggcctatc caacatgtga gcccatccca aatgaaatga agcaataaaa
     4141 ataaatctcc agtcccc
//
