LOCUS       NM_176958               6190 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus hypoxia-inducible factor 1, alpha subunit inhibitor
            (Hif1an), mRNA.
ACCESSION   NM_176958
VERSION     NM_176958.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Bargiela,D., Cunha,P.P., Velica,P., Krause,L.C.M., Brice,M.,
            Barbieri,L., Gojkovic,M., Foskolou,I.P., Rundqvist,H. and
            Johnson,R.S.
  TITLE     The factor inhibiting HIF regulates T cell differentiation and
            anti-tumour efficacy
  JOURNAL   Front Immunol 15, 1293723 (2024)
   PUBMED   38690263
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Ma,J., Al Moussawi,K., Lou,H., Chan,H.F., Wang,Y., Chadwick,J.,
            Phetsouphanh,C., Slee,E.A., Zhong,S., Leissing,T.M., Roth,A.,
            Qin,X., Chen,S., Yin,J., Ratnayaka,I., Hu,Y., Louphrasitthiphol,P.,
            Taylor,L., Bettencourt,P.J.G., Muers,M., Greaves,D.R., McShane,H.,
            Goldin,R., Soilleux,E.J., Coleman,M.L., Ratcliffe,P.J. and Lu,X.
  TITLE     Deficiency of factor-inhibiting HIF creates a tumor-promoting
            immune microenvironment
  JOURNAL   Proc Natl Acad Sci U S A 121 (10), e2309957121 (2024)
   PUBMED   38422022
  REMARK    GeneRIF: Deficiency of factor-inhibiting HIF creates a
            tumor-promoting immune microenvironment.
REFERENCE   3  (bases 1 to 6190)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 6190)
  AUTHORS   Cai,X., Wang,R., Zhu,J., Li,X., Liu,X., Ouyang,G., Wang,J., Li,Z.,
            Zhu,C., Deng,H. and Xiao,W.
  TITLE     Factor inhibiting HIF negatively regulates antiviral innate
            immunity via hydroxylation of IKK
  JOURNAL   Cell Rep 43 (1), 113606 (2024)
   PUBMED   38127621
  REMARK    Erratum:[Cell Rep. 2024 Feb 27;43(2):113755. doi:
            10.1016/j.celrep.2024.113755.. PMID: 38306269]
REFERENCE   5  (bases 1 to 6190)
  AUTHORS   Gupta,N., Soriano-Ubeda,C., Stein,P., Savy,V., Papas,B.N.,
            Ardestani,G., Carvacho,I., Alfandari,D., Williams,C.J. and
            Fissore,R.A.
  TITLE     Essential role of Mg2+ in mouse preimplantation embryo development
            revealed by TRPM7 chanzyme-deficient gametes
  JOURNAL   Cell Rep 42 (10), 113232 (2023)
   PUBMED   37824328
REFERENCE   6  (bases 1 to 6190)
  AUTHORS   Kuzmanov,A., Wielockx,B., Rezaei,M., Kettelhake,A. and Breier,G.
  TITLE     Overexpression of factor inhibiting HIF-1 enhances vessel
            maturation and tumor growth via platelet-derived growth factor-C
  JOURNAL   Int J Cancer 131 (5), E603-E613 (2012)
   PUBMED   22095574
  REMARK    GeneRIF: Overexpression of factor inhibiting HIF-1 enhances vessel
            maturation and tumor growth via platelet-derived growth factor-C in
            osteosarcoma.
REFERENCE   7  (bases 1 to 6190)
  AUTHORS   Singleton,R.S., Trudgian,D.C., Fischer,R., Kessler,B.M.,
            Ratcliffe,P.J. and Cockman,M.E.
  TITLE     Quantitative mass spectrometry reveals dynamics of
            factor-inhibiting hypoxia-inducible factor-catalyzed hydroxylation
  JOURNAL   J Biol Chem 286 (39), 33784-33794 (2011)
   PUBMED   21808058
  REMARK    GeneRIF: Quantitative mass spectrometry reveals dynamics of
            factor-inhibiting hypoxia-inducible factor-catalyzed hydroxylation.
REFERENCE   8  (bases 1 to 6190)
  AUTHORS   Schodel,J., Bohr,D., Klanke,B., Schley,G., Schlotzer-Schrehardt,U.,
            Warnecke,C., Kurtz,A., Amann,K., Eckardt,K.U. and Willam,C.
  TITLE     Factor inhibiting HIF limits the expression of hypoxia-inducible
            genes in podocytes and distal tubular cells
  JOURNAL   Kidney Int 78 (9), 857-867 (2010)
   PUBMED   20720525
  REMARK    GeneRIF: FIH1 appears to be a suppressor of oxygen-dependent genes
            in the kidney, operating through HIF-dependent and -independent
            mechanisms.
REFERENCE   9  (bases 1 to 6190)
  AUTHORS   Zhang,N., Fu,Z., Linke,S., Chicher,J., Gorman,J.J., Visk,D.,
            Haddad,G.G., Poellinger,L., Peet,D.J., Powell,F. and Johnson,R.S.
  TITLE     The asparaginyl hydroxylase factor inhibiting HIF-1alpha is an
            essential regulator of metabolism
  JOURNAL   Cell Metab 11 (5), 364-378 (2010)
   PUBMED   20399150
  REMARK    GeneRIF: Lackin of hypoxia-inducible factor 1 alpha subunit
            inhibitor exhibit reduced body weight, elevated metabolic rate,
            hyperventilation, and improved glucose and lipid homeostasis and
            are resistant to high-fat-diet-induced weight gain and hepatic
            steatosis.
REFERENCE   10 (bases 1 to 6190)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY122345.1, BY713373.1, CN535711.1 and AC151478.1.
            
            On Jul 16, 2005 this sequence version replaced NM_176958.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154774.1, SRR1660819.47759.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                BY122345.1         5-19
            16-664              BY713373.1         2-650
            665-1045            CN535711.1         249-629
            1046-6190           AC151478.1         153388-158532
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 37.98 cM"
     gene            1..6190
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /note="hypoxia-inducible factor 1, alpha subunit
                     inhibitor"
                     /db_xref="GeneID:319594"
                     /db_xref="MGI:MGI:2442345"
     exon            1..201
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     CDS             25..1074
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /EC_number="1.14.11.30"
                     /EC_number="1.14.11.n4"
                     /note="hypoxia-inducible factor asparagine hydroxylase"
                     /codon_start=1
                     /product="hypoxia-inducible factor 1-alpha inhibitor"
                     /protein_id="NP_795932.2"
                     /db_xref="CCDS:CCDS29852.1"
                     /db_xref="GeneID:319594"
                     /db_xref="MGI:MGI:2442345"
                     /translation="MAATAAEVAASGSGEAREEAEAPGPAWDESQLRSYSFPTRPIPR
                     LSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKFLY
                     YDEKKMGNFQNFKPRSNREEIKFHEFVEKLQAIQQRGGEERLYLQQTLNDTVGRKIVM
                     DFLGFNWNWINKQQGKRGWGQLTSNLLLIGMEGNVTPAHYDEQQNFFAQIKGHKRCIL
                     FPPDQFECLYPYPVHHPCDRQSQVDFDNPDYERFPNFRNVVGYETVVGPGDVLYIPMY
                     WWHHIESLLNGGITITVNFWYKGAPTPKRIEYPLKAHQKVAIMRNIEKMLGEALGNPQ
                     EVGPLLNTMIKGRYN"
     exon            202..452
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            453..601
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            602..747
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            748..854
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            855..918
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            919..1029
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..6190
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2811..2816
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /note="hexamer: AATACA"
     polyA_site      2846
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
     regulatory      6163..6168
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /note="hexamer: AATAAA"
     polyA_site      6190
                     /gene="Hif1an"
                     /gene_synonym="2310046M24Rik; A830014H24Rik; FIH; FIH1"
                     /note="major polyA site"
ORIGIN      
        1 ggcggggccg tccctagagt agagatggcg gcgacggcag ccgaggttgc ggcctctggc
       61 tctggagagg cccgggaaga agccgaagct ccgggtcccg cctgggatga gtcccagcta
      121 cgaagttaca gctttccgac caggcccatc ccacgtctga gccagagtga ccctcgggcg
      181 gaagagctca tcgaaaatga ggagccagtg gtgctgaccg acacaaacct tgtgtatcct
      241 gcactgaagt gggacctcga atacctgcaa gagaatattg gcaacggaga tttctctgtg
      301 tacagtgcca gcacccataa gttcttatac tatgatgaga agaagatggg caatttccag
      361 aactttaagc caaggtccaa cagggaggaa attaaatttc atgagttcgt tgagaaacta
      421 caagctatac agcagcgcgg gggagaagag cggttgtatc tgcagcagac actcaatgac
      481 accgtgggga ggaagattgt catggacttc ttgggtttta actggaactg gatcaataaa
      541 cagcagggga aacgcggctg ggggcagctg acctctaacc tgttgctcat tggcatggaa
      601 ggaaatgtga cacctgctca ctatgatgag cagcagaact tttttgccca aataaaaggc
      661 cataagagat gcatcttatt tcctccggat cagttcgagt gcctctatcc ataccctgtc
      721 catcacccct gtgacaggca gagccaggtg gactttgaca atcctgacta cgagaggttc
      781 cccaatttcc gaaatgtggt tggttatgag acagtggttg gccctgggga tgttctttac
      841 atcccaatgt actggtggca ccatatagag tcattactaa atgggggaat taccatcact
      901 gtgaacttct ggtataaggg ggctcccacc cctaagagaa tcgaatatcc tctcaaagct
      961 catcagaaag tagccatcat gagaaacatt gagaagatgc ttggagaggc cttggggaac
     1021 ccacaagagg tggggccttt gttgaacacc atgattaaag gccgttacaa ctaacctgcc
     1081 agaggtcaag gcctcctgtc aggtgactgc tatcctgtcc acatgcttcg ttgatgagga
     1141 caggacactc caagcactag tattgcacgc tgcacttaat ggactggact cttgccatgg
     1201 ccctggaggc aggtgtctga ggcaaggcag ggtagttgac tccactccca tttggaaagg
     1261 tttctcaccc ttgcctcttg agccccagaa ccttccctct gcccccctga agtcctgcat
     1321 ttagtgtgtg gagtcccagc ttctggttgt aatcatgtct gtgttagtct gttaacctca
     1381 gggtgtgtgt gtgtgtgtac atgtgtgcat atgcatgtgt gtatacacac acatgcatgt
     1441 atctgttcct tgttccctct tcctgggtca ggatgtcact tctggctctc agctcctgtc
     1501 tcctgaagcc tcagtgcctc agcctgggag gagagatgct cctgtgtccc tacaacatct
     1561 gggctgtggt gccagaatga ctctgcacat gagtctgcct tctgccagtt tttgcagcat
     1621 agtcaggcta caaaccaggt ggcaataact gctggggccc taatggggac attgagaagg
     1681 aggcttatct ttgggaggta gccttctagt ggaagaggat tagataagat tccagttgag
     1741 aactacatgc ttaagccata catagaagga aaacttgggg acctgaaacc aggattgtgc
     1801 atatgtccaa atacaggcat ttgcagccgt tctcttcagt accagctcct gtccctgtgc
     1861 tgcaatgagg gattctagct ctgacttttt tgggctcgag gggtatgaac ctctgcatgt
     1921 gtgtgtacac atctgcacac ttgtatgcat gtgtgatcac acttgccatc cttcttgctt
     1981 actaaggttc tcaactgcta acattgtcca gtgtggcagt acagcgtgaa ccccagatac
     2041 tgcctgactc agcctagagc ttttacactc ggattttagc catcccgtct tggtcagatc
     2101 ttactgcagc ctacctaaag ctgactggct caagcctccc agtcctgtgt ctttccctgt
     2161 ctgaaccaca tccctgctcc tgctgagctt cctggctgag tagatgaaat ggggtcagac
     2221 ttaggcagct aactcaatac ctttcatcca cctcagggca aggggaaaaa aaatgtcctc
     2281 ttgcctctta gagccagcgc ctctgccaga cccaacaaag cgtcgcatgt gagctggagt
     2341 agagctggga gagcttggag atagaattta cttttctgtt ggtagctgtt tatgggagcc
     2401 ctcctcgggg ctgcagctta tagctctgtg ttacacaaca cagagcgcaa tcatttgtat
     2461 ctgttaagag ggaatggggc cccagcagct tggcagagtg gtggccccgt gtccacttac
     2521 ctcaagtccc ttccttctct gtcaaccttt tttctctaaa attggagttg gaagtctgat
     2581 tttattcttt tctttgccac cttttagtcc ccttccctgt agatttgacc tcccgtgttc
     2641 aagctgtggg tcttagccct gaagctcagc ttacagcgcc ccatggactg aacagatgca
     2701 ggcgaatgct tgtgcacccg tgtgcgttta ggggcgggag ctctagcccc cactctgggc
     2761 tctgcagtgt ggttgcgcag acttggtacc ttcttgagta tggctcttta aatacagccc
     2821 gtgctgggca gtgtgatcgc ggcaaacgca tgcctccatc tcttttggga acgagggatg
     2881 cagcagaaga gggaggcaga gtggtattag tttggccacc gtggcggatt ttcattctcc
     2941 gcagatctat ggtcctggct ctgccctcaa aggtgtctgg gatgtgtggc agggagtctg
     3001 gatctgctcg gttgaccctt cagtataact ctgctctcac aggtctgacc tagggtggct
     3061 gagggcagcg agagagaagg tgagcttagg caccatcaca ggattggccc tgatgctggg
     3121 atgatgtcat ggctgttcct ggtcttagac acctgatgtg tacctgttgt ttctagctct
     3181 gtccctcaca gtgctgcgtg gtagtcaaga gcaaacctaa tgccatttag ctataaaccc
     3241 actactccct gctcctccat ttcttccaaa tgctgttggt tctctacctc acctcaagcc
     3301 ttgttcccta gagagtgtgc aggtagactc aggcaagagg gtgtggaagg gtgaaaggct
     3361 ttgggggttc agctagagac atggaaaagc tgcctagaaa gccatcttaa caagctaagg
     3421 gttctgggaa gaagcagaag agtgagcttc ggccctgcgg gggaaagggg tgctgtaggt
     3481 cctgtagctc ctctcaccac acctctgcat gctgagagga agctctggta acccgattca
     3541 tattctgctg ggccacttcc tcctggggat ggcaagcagg gactcaccta ggctagggtc
     3601 tcaaccctag ggctgttgag gctccagttg tacttgcagc agagcaggca tcactattgt
     3661 gtacccattc tagccaacag cctgggagtc agacgtgcca aggaaggtgg agacctgggc
     3721 ttgtccaggg gtcagccatc ttgcaggctc tcgtgtttat accagcagta cagtgaaaac
     3781 tactgttttg tgaagtcaaa tatttgctgg ggggttagag ttatggggtg gaggaggggc
     3841 tgttctgggc atcagttgag cagatgccca ggatgcctgg gggagaccag cttcccctac
     3901 aaatcagagc tctaaattac aaggttttta ccaacgcgaa cagttggggg aagtcgtctg
     3961 ctctcatttg cgtaatggtt tctgtcactg gtgattagac acaggatgaa ggaaaagaaa
     4021 tttgacaatt aggaatgagc catcattaat ctgaatctgt taggagacag agaggggaag
     4081 gctccttact attgggccaa cctagtatgg ggagacactc cctatttctg accccagaat
     4141 cttgtgggcc aggtaccctt ttggagttcc cacaagtgtg ggtggagttt gtatgccttc
     4201 atagactgga gtccaggccc agcttgaggt gggtttgcaa acagacatct ttctgcctga
     4261 gccctcgcag ttgaaggtag gtaggctggt tactgcgtgt gctcagttac aggaaggatc
     4321 tctgtagtag tcagcactgt tgcgccccac ctgtgacagg gatctgtcac ttcgaagcac
     4381 ttgagcttgt gtgggaagcc tgagcttcat caaaccccag acactggtgg aagttggaaa
     4441 ggaaaccgta tgaagagtag cagagcctag gtatgtgcaa ggaggggtgt gaggaagagc
     4501 tggtccagca caggctgtct gggaggagac tcggcctctc agcagcgttg tcttactctg
     4561 gatcagcgag ccattgctgt ggggaacact gaggttcctg gctcagtgac ccagtgatgt
     4621 ggctattgaa aaatggagag tccagcgtag atcttccagt gtttgcaagt tacctcacca
     4681 ctcaccccca tcccttcaaa cgcctaagaa tattagtctg taccaagtag tggtcctcac
     4741 cattagtgtg ttccttagac cccagccccc ccaaggtttg gagggggctc ctctgtctcc
     4801 tttcataatg tgaacttctc aaggatggtg ctatctcagt ccttggtaag ttccctacct
     4861 ctgttgtctt tcatccttca aaacttggtg cagaatttag caagggctac cagcagtgac
     4921 cctctgacca gtttttccta catggccagg ggtggggatg gggatagctc agggttgtag
     4981 aggccaggcc tttccagttc ctgtttctct tggtgtcctc ttgacccatc tttaagagga
     5041 tccttgagct ttctcctaac taggctctca gtggcctctt taccaaggct cttttcctca
     5101 aagacgctct ctggctccaa gtgctgggca gcctggctgg aggatccctc tccacagggt
     5161 ctgtccctgc ccctgcttca cttctgacct caggtagctc tgttgctgtg tccctgctga
     5221 gttgctatgt ccgtctgctc tgtccactta ccattcctca gtctttccat gctgggccgt
     5281 tgtcttacca tggccatcct tcctaccttt tcttcagcca gctctgttta attatagacc
     5341 catagggtcc tgactggagc cccattgagc actttgggga cgtttctgga ggaacagagg
     5401 ctcagaaact ggtgtcctca aggcccatgg gcctgtggtt agcgaagcct cggagacaac
     5461 tggggaggaa cttggagagg tgaaggactt ggtgagagac ttggagaaag aggccaggct
     5521 tgggaaagca gggtggcagg agcgagctct gttttccatg agtgtgtcct tgtgccttag
     5581 ggtccagcag ttcaggtatg aggggcattt tctgaatgcc tcctaatttc tctcaggtct
     5641 ttggctcagt gtcaccaagt ttgacagacc ccttcgttcc tgagcttgct agaagggaag
     5701 cagcacgttg agtggtgtgg tgtggtgcga gagcggaggc ctcccatgca gaaggctggg
     5761 gtggtggaca agagaaaggg cccggtggtc agcttctcac cgatgactct ccatgcctca
     5821 agagactgag tatagtagtt ggttcaagaa gttactctaa aactgcctgc cctcttcacc
     5881 taaagtctgt tctcatttcg gggaggaatg caagtcctta attttcaagg gaagacagtc
     5941 tagtgtttaa aagcatgtgt ctggggtctg gtccctttgg tacatttcta gcttgctact
     6001 taccagctgg gtttgacctt ggttgattga ttgaacccga gtctcggttt cctttcctat
     6061 aatgacttgt gggggtgggg gtttgaaaat atctacctca gggttgctgg atgaactgaa
     6121 ataatgtctg taaagcttta gcacagtgcc tggcaagcac ttaataaacg gctgtggtgg
     6181 tggtggttta
//
