LOCUS       NM_144881               2857 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus hedgehog acyltransferase (Hhat), mRNA.
ACCESSION   NM_144881
VERSION     NM_144881.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Asciolla,J.J. and Resh,M.D.
  TITLE     Hedgehog Acyltransferase Promotes Uptake of Palmitoyl-CoA across
            the Endoplasmic Reticulum Membrane
  JOURNAL   Cell Rep 29 (13), 4608-4619 (2019)
   PUBMED   31875564
  REMARK    GeneRIF: Hedgehog Acyltransferase Promotes Uptake of Palmitoyl-CoA
            across the Endoplasmic Reticulum Membrane.
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Iulianella,A., Sakai,D., Kurosaka,H. and Trainor,P.A.
  TITLE     Ventral neural patterning in the absence of a Shh activity gradient
            from the floorplate
  JOURNAL   Dev Dyn 247 (1), 170-184 (2018)
   PUBMED   28891097
REFERENCE   3  (bases 1 to 2857)
  AUTHORS   Yun,K. and Perantoni,A.O.
  TITLE     Hydronephrosis in the Wnt5a-ablated kidney is caused by an abnormal
            ureter-bladder connection
  JOURNAL   Differentiation 94, 1-7 (2017)
   PUBMED   27923152
REFERENCE   4  (bases 1 to 2857)
  AUTHORS   Kurosaka,H., Trainor,P.A., Leroux-Berger,M. and Iulianella,A.
  TITLE     Cranial nerve development requires co-ordinated Shh and canonical
            Wnt signaling
  JOURNAL   PLoS One 10 (3), e0120821 (2015)
   PUBMED   25799573
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2857)
  AUTHORS   Callier,P., Calvel,P., Matevossian,A., Makrythanasis,P.,
            Bernard,P., Kurosaka,H., Vannier,A., Thauvin-Robinet,C., Borel,C.,
            Mazaud-Guittot,S., Rolland,A., Desdoits-Lethimonier,C.,
            Guipponi,M., Zimmermann,C., Stevant,I., Kuhne,F., Conne,B.,
            Santoni,F., Lambert,S., Huet,F., Mugneret,F., Jaruzelska,J.,
            Faivre,L., Wilhelm,D., Jegou,B., Trainor,P.A., Resh,M.D.,
            Antonarakis,S.E. and Nef,S.
  TITLE     Loss of function mutation in the palmitoyl-transferase HHAT leads
            to syndromic 46,XY disorder of sex development by impeding Hedgehog
            protein palmitoylation and signaling
  JOURNAL   PLoS Genet 10 (5), e1004340 (2014)
   PUBMED   24784881
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2857)
  AUTHORS   Abe,Y., Kita,Y. and Niikura,T.
  TITLE     Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol
            uptake/transporter 1, acts as a negative regulator for N-terminal
            palmitoylation of Sonic hedgehog
  JOURNAL   FEBS J 275 (2), 318-331 (2008)
   PUBMED   18081866
REFERENCE   7  (bases 1 to 2857)
  AUTHORS   Li,C., Xu,X., Nelson,D.K., Williams,T., Kuehn,M.R. and Deng,C.X.
  TITLE     FGFR1 function at the earliest stages of mouse limb development
            plays an indispensable role in subsequent autopod morphogenesis
  JOURNAL   Development 132 (21), 4755-4764 (2005)
   PUBMED   16207751
REFERENCE   8  (bases 1 to 2857)
  AUTHORS   Coumoul,X., Shukla,V., Li,C., Wang,R.H. and Deng,C.X.
  TITLE     Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA
            interference
  JOURNAL   Nucleic Acids Res 33 (11), e102 (2005)
   PUBMED   15987787
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2857)
  AUTHORS   Chen,M.H., Li,Y.J., Kawakami,T., Xu,S.M. and Chuang,P.T.
  TITLE     Palmitoylation is required for the production of a soluble
            multimeric Hedgehog protein complex and long-range signaling in
            vertebrates
  JOURNAL   Genes Dev 18 (6), 641-659 (2004)
   PUBMED   15075292
REFERENCE   10 (bases 1 to 2857)
  AUTHORS   Nelson,D.K. and Williams,T.
  TITLE     Frontonasal process-specific disruption of AP-2alpha results in
            postnatal midfacial hypoplasia, vascular anomalies, and nasal
            cavity defects
  JOURNAL   Dev Biol 267 (1), 72-92 (2004)
   PUBMED   14975718
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL365317.22, AC115917.11 and AC105325.11.
            
            On Dec 21, 2021 this sequence version replaced NM_144881.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028230.1, SRR7345562.2403635.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164135 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-259               AL365317.22        1786-2044
            260-389             AL365317.22        13224-13353
            390-457             AL365317.22        24076-24143
            458-571             AL365317.22        31401-31514
            572-766             AL365317.22        45546-45740
            767-1000            AL365317.22        46671-46904
            1001-1172           AL365317.22        55896-56067
            1173-1323           AL365317.22        79075-79225
            1324-1359           AC115917.11        160126-160161       c
            1360-1561           AC115917.11        93557-93758         c
            1562-1706           AC115917.11        51609-51753         c
            1707-2857           AC105325.11        114657-115807       c
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 97.55 cM"
     gene            1..2857
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /note="hedgehog acyltransferase"
                     /db_xref="GeneID:226861"
                     /db_xref="MGI:MGI:2444681"
     exon            1..259
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            260..389
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..1798
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /note="skinny hedgehog protein"
                     /codon_start=1
                     /product="protein-cysteine N-palmitoyltransferase HHAT"
                     /protein_id="NP_659130.2"
                     /db_xref="CCDS:CCDS15628.1"
                     /db_xref="GeneID:226861"
                     /db_xref="MGI:MGI:2444681"
                     /translation="MLPGWELTLCLLVSLGFHFRSFYEVYKVSREHEEELDQEFELEM
                     DTLFGGLKKDPTDFEWNFWMEWGKRRLVWLFIGHMAVSQLATLLTKKHRPWIVMVYGM
                     WACWCVLGAPGVVMVLLHSTIAFCVAQFRSVLLSWLCSLLLLSTLRLQSVEEVKRRWY
                     KTENEYYLLQFTLTVRCLYYTSFSLELCRQPPSAQPTPSAQGASHSYPWLLTYVFYYP
                     VFHNGPILNFPEFFRQMQQPELNSLQHSLCIVAKGLGRLLCWWWLAELMVHLMYMHAL
                     YSSAPLLESVSCWTLGGLALAQVLFFYVKYLVLFGVPALLMRLDGLTPPPLPRCVSTM
                     FSFTGMWRYFDVGLHNFLIRYVYIPLGGSQHGLLGTLLSTATTFAFVSYWHGSYEDLW
                     CWAALNWLGVTVESGVRRLLETPCVRETLARHLSPQAHHRLHALLAACSTSMLILFNL
                     VFLGGIQVGKTYWNRIFLQGWPWVTLSVLGFLYCYSHVDIAWAQTYTVL"
     exon            390..457
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            458..571
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            572..766
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            767..1000
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1172
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1323
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1359
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1561
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1706
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..2857
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2836..2841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /note="hexamer: AATAAA"
     polyA_site      2857
                     /gene="Hhat"
                     /gene_synonym="2810432O22Rik; AP-2CRE; Skn;
                     Tg(TFAP2A-cre)1Will"
                     /note="major polyA site"
ORIGIN      
        1 attcctttgg ggaccggaag aatcctggag cctgaggttc tggtgggacc ctgtgtaatg
       61 attcggctcc agtaaatgta cagagccctg gccaatcctg cgttcgggaa cccgggcaca
      121 acatctcagg ccaggcgctc acccccgcgg cctctgaccc gcgctgtgct cgcgagtccc
      181 tcccgggccg cggctcagcg cggcggggtg cagggacgcc ttctctcccg ctcgcccagc
      241 tctgcacact gcgatccaga aactctccac actacactgg gaagcagaga ctccagctat
      301 gctgcccggg tgggaactga ctctctgcct gcttgtttcc ttgggtttcc acttccgctc
      361 tttctatgaa gtttacaaag tctccagaga gcatgaagag gaactggatc aagaatttga
      421 gctggagatg gacactctat ttggaggact gaagaaggac ccgactgatt ttgagtggaa
      481 cttctggatg gaatggggga agcggaggct ggtgtggctc ttcattggcc atatggctgt
      541 gtctcagttg gccacgttac tcacaaagaa gcacagaccc tggattgtca tggtctatgg
      601 aatgtgggcc tgctggtgtg tgctgggggc gccaggcgtg gtcatggtcc ttctccacag
      661 caccatcgcg ttctgcgtgg ctcagttccg atccgtgctc ctgtcctggc tgtgttctct
      721 ccttctgctc tccaccctaa ggctgcaaag tgtggaggaa gttaagagaa ggtggtacaa
      781 gacagagaac gagtattacc tgctccagtt tacgctcact gttcgctgcc tgtactacac
      841 cagcttcagc ctggagctgt gccggcagcc tccgtctgca caacctactc catctgcgca
      901 aggtgcctcc cattcctacc cgtggctgct gacctacgtc ttctattatc ccgtcttcca
      961 caacgggccc atccttaact tcccggagtt cttcagacag atgcagcagc cagagctcaa
     1021 ctctctgcag cacagcctct gcattgtagc caagggcctg ggtcgtctgc tctgctggtg
     1081 gtggctggct gaactgatgg tacacctcat gtacatgcat gccctctaca gcagcgcccc
     1141 cctcctggag agcgtttcct gctggacctt aggaggactg gcgctggccc aagtgctctt
     1201 cttctatgtg aagtacctgg tgctctttgg tgtcccggct ctgctcatgc gcctggatgg
     1261 actcacgcca ccacctcttc cccgctgcgt gagcaccatg ttcagtttta ccgggatgtg
     1321 gaggtatttt gatgttggac tacacaattt cttaatcagg tatgtgtaca tcccactggg
     1381 cgggtcccag catggcctgt tggggacact gctttctact gcaacaacgt ttgcgttcgt
     1441 gagctactgg catggtagct atgaggacct ctggtgctgg gcagcgctca actggctggg
     1501 agtcactgtg gagagtgggg tccggaggct gctagagaca ccctgtgtcc gggagacttt
     1561 ggcccggcac ctctccccac aagcccacca tcgactgcac gctctgcttg ccgcctgctc
     1621 tacctccatg ctcatcctct tcaacctggt gttcctcggg ggaattcaag ttgggaaaac
     1681 ctactggaac aggatcttcc tgcaagggtg gccatgggtg actctgtctg tcctgggatt
     1741 cctgtactgc tattctcacg tggacattgc ttgggctcag acatacacag ttctttaact
     1801 gtgtatggct gctgggccag ctcagcctcc aagggacatg gcatttcatc ctgatctctc
     1861 attctgagac catcctcaaa ccaccctcca ctccaggaca gtccctgagg gaccaccctc
     1921 cactccagga cagtccctga gggaccaccc tccactccag gacagtccct gagggaccac
     1981 cctccactcc aggacagtcc ctgagggacc accctccact ccaggacagt ccctgaggga
     2041 ctaccttcca ctcatcttct aatgaagacg cttcattcca ggactggcag cttcatcaat
     2101 cacagtaaat acacaactca aacttctagc tctggagcct cagagatttg tcgcgtcccc
     2161 cccagaagct tcctgacgca caaggtacag tccagagcag tctcaggttt tcctgactat
     2221 acctgtgata tttgttccca tgaacctgct tgtttttttt tttttttctt ctctaccaaa
     2281 gcttaggtgt ggcagatgtg tgttttgata tggaagtcct ggttagggat aggcttgcca
     2341 tccttgggat gtctttctga gaaattctga gatactttga tgtctccttt ctatgtgctg
     2401 ccctgggggc tctggactaa ccttcgcatg aatggtcctt agacagggga tcagcagaac
     2461 catttcacat ttcaagtggc ccagctagag tgggtgatgt gcagctgggg ctttacaaag
     2521 agttctgtaa cttgaacttt attggagagt ccacacaggg ctgctgcttt ctgagacttg
     2581 tagccatgga ggaacatggt ggttcctctc acagctgttc tgcacagaag tggagtttaa
     2641 aagccagaga ataagatgtc cacatgcagg tggtgttgcg caaggcctct ggggagagtg
     2701 acatcccaag gtcccatgac ttttactcag tgggttttat tgccttattc tcatttaaaa
     2761 aaaaaaaaaa cagaacctgt ttttgctaca tagataattc tgattttttt gtggtcttat
     2821 tttggatatt ttttaaataa aaattttgag atctaaa
//
