LOCUS       NM_029884               4603 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus heparan-alpha-glucosaminide N-acetyltransferase
            (Hgsnat), mRNA.
ACCESSION   NM_029884
VERSION     NM_029884.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Pan,X., Caillon,A., Fan,S., Khan,S., Tomatsu,S. and
            Pshezhetsky,A.V.
  TITLE     Heterologous HSPC Transplantation Rescues Neuroinflammation and
            Ameliorates Peripheral Manifestations in the Mouse Model of
            Lysosomal Transmembrane Enzyme Deficiency, MPS IIIC
  JOURNAL   Cells 13 (10), 877 (2024)
   PUBMED   38786099
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 4603)
  AUTHORS   Taherzadeh,M., Zhang,E., Londono,I., De Leener,B., Wang,S.,
            Cooper,J.D., Kennedy,T.E., Morales,C.R., Chen,Z., Lodygensky,G.A.
            and Pshezhetsky,A.V.
  TITLE     Severe central nervous system demyelination in Sanfilippo disease
  JOURNAL   Front Mol Neurosci 16, 1323449 (2023)
   PUBMED   38163061
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4603)
  AUTHORS   Ludwig,J., Sawant,O.B., Wood,J., Singamsetty,S., Pan,X.,
            Bonilha,V.L., Rao,S. and Pshezhetsky,A.V.
  TITLE     Histological characterization of retinal degeneration in
            mucopolysaccharidosis type IIIC
  JOURNAL   Exp Eye Res 229, 109433 (2023)
   PUBMED   36858249
REFERENCE   5  (bases 1 to 4603)
  AUTHORS   Pan,X., Taherzadeh,M., Bose,P., Heon-Roberts,R., Nguyen,A.L.A.,
            Xu,T., Para,C., Yamanaka,Y., Priestman,D.A., Platt,F.M., Khan,S.,
            Fnu,N., Tomatsu,S., Morales,C.R. and Pshezhetsky,A.V.
  TITLE     Glucosamine amends CNS pathology in mucopolysaccharidosis IIIC
            mouse expressing misfolded HGSNAT
  JOURNAL   J Exp Med 219 (8) (2022)
   PUBMED   35704026
REFERENCE   6  (bases 1 to 4603)
  AUTHORS   Haer-Wigman,L., Newman,H., Leibu,R., Bax,N.M., Baris,H.N.,
            Rizel,L., Banin,E., Massarweh,A., Roosing,S., Lefeber,D.J.,
            Zonneveld-Vrieling,M.N., Isakov,O., Shomron,N., Sharon,D., Den
            Hollander,A.I., Hoyng,C.B., Cremers,F.P. and Ben-Yosef,T.
  TITLE     Non-syndromic retinitis pigmentosa due to mutations in the
            mucopolysaccharidosis type IIIC gene, heparan-alpha-glucosaminide
            N-acetyltransferase (HGSNAT)
  JOURNAL   Hum Mol Genet 24 (13), 3742-3751 (2015)
   PUBMED   25859010
REFERENCE   7  (bases 1 to 4603)
  AUTHORS   Cameron,T.L., Gresshoff,I.L., Bell,K.M., Pirog,K.A., Sampurno,L.,
            Hartley,C.L., Sanford,E.M., Wilson,R., Ermann,J.,
            Boot-Handford,R.P., Glimcher,L.H., Briggs,M.D. and Bateman,J.F.
  TITLE     Cartilage-specific ablation of XBP1 signaling in mouse results in a
            chondrodysplasia characterized by reduced chondrocyte proliferation
            and delayed cartilage maturation and mineralization
  JOURNAL   Osteoarthritis Cartilage 23 (4), 661-670 (2015)
   PUBMED   25600960
REFERENCE   8  (bases 1 to 4603)
  AUTHORS   Martins,C., Hulkova,H., Dridi,L., Dormoy-Raclet,V., Grigoryeva,L.,
            Choi,Y., Langford-Smith,A., Wilkinson,F.L., Ohmi,K., DiCristo,G.,
            Hamel,E., Ausseil,J., Cheillan,D., Moreau,A., Svobodova,E.,
            Hajkova,Z., Tesarova,M., Hansikova,H., Bigger,B.W., Hrebicek,M. and
            Pshezhetsky,A.V.
  TITLE     Neuroinflammation, mitochondrial defects and neurodegeneration in
            mucopolysaccharidosis III type C mouse model
  JOURNAL   Brain 138 (Pt 2), 336-355 (2015)
   PUBMED   25567323
  REMARK    Erratum:[Brain. 2015 Jul;138(Pt 7):e366. doi:
            10.1093/brain/awv035.. PMID: 26106098]
REFERENCE   9  (bases 1 to 4603)
  AUTHORS   Hrebicek,M., Mrazova,L., Seyrantepe,V., Durand,S., Roslin,N.M.,
            Noskova,L., Hartmannova,H., Ivanek,R., Cizkova,A., Poupetova,H.,
            Sikora,J., Urinovska,J., Stranecky,V., Zeman,J., Lepage,P.,
            Roquis,D., Verner,A., Ausseil,J., Beesley,C.E., Maire,I.,
            Poorthuis,B.J., van de Kamp,J., van Diggelen,O.P., Wevers,R.A.,
            Hudson,T.J., Fujiwara,T.M., Majewski,J., Morgan,K., Kmoch,S. and
            Pshezhetsky,A.V.
  TITLE     Mutations in TMEM76* cause mucopolysaccharidosis IIIC (Sanfilippo C
            syndrome)
  JOURNAL   Am J Hum Genet 79 (5), 807-819 (2006)
   PUBMED   17033958
REFERENCE   10 (bases 1 to 4603)
  AUTHORS   Fan,X., Zhang,H., Zhang,S., Bagshaw,R.D., Tropak,M.B.,
            Callahan,J.W. and Mahuran,D.J.
  TITLE     Identification of the gene encoding the enzyme deficient in
            mucopolysaccharidosis IIIC (Sanfilippo disease type C)
  JOURNAL   Am J Hum Genet 79 (4), 738-744 (2006)
   PUBMED   16960811
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC093366.9 and AC122752.10.
            
            On Mar 14, 2022 this sequence version replaced NM_029884.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK035264.1, AK152926.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-152               AC093366.9         187378-187529
            153-268             AC093366.9         191097-191212
            269-405             AC093366.9         192352-192488
            406-527             AC093366.9         192886-193007
            528-597             AC093366.9         195622-195691
            598-667             AC093366.9         199191-199260
            668-771             AC093366.9         200596-200699
            772-848             AC093366.9         203152-203228
            849-879             AC093366.9         204441-204471
            880-1040            AC093366.9         206704-206864
            1041-1156           AC093366.9         208265-208380
            1157-1278           AC093366.9         209327-209448
            1279-1405           AC093366.9         210647-210773
            1406-1492           AC093366.9         211999-212085
            1493-1570           AC093366.9         216043-216120
            1571-1641           AC093366.9         217537-217607
            1642-1754           AC093366.9         217706-217818
            1755-4162           AC093366.9         218739-221146
            4163-4603           AC122752.10        155037-155477       c
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 14.22 cM"
     gene            1..4603
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /note="heparan-alpha-glucosaminide N-acetyltransferase"
                     /db_xref="GeneID:52120"
                     /db_xref="MGI:MGI:1196297"
     exon            1..152
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..1936
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /EC_number="2.3.1.78"
                     /note="transmembrane protein 76"
                     /codon_start=1
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     precursor"
                     /protein_id="NP_084160.2"
                     /db_xref="CCDS:CCDS40309.1"
                     /db_xref="GeneID:52120"
                     /db_xref="MGI:MGI:1196297"
                     /translation="MGAGPALAALLLAGSVLSATLLAPGRRAEPDLDEKRNVELKMDQ
                     ALLLIHNELLGTSLTVYWKSDDCYQCTFQPLANVSHGGKPAKPSVAPVSVSTQHGSIL
                     QVNSTSEERAACRLEYKFGEFGNYSLLVQHASSGANKIACDIIVNENPVDSNLPVSIA
                     FLVGLALIVAVSLLRLLLSLDDVNNWISKTIASRETDRLINSELGSPSRADPLSADYQ
                     PETRRSSANRLRCVDTFRGLALVLMVFVNYGGGKYWYFKHSSWNGLTVADLVFPWFVF
                     IMGTSIFLSMTSILQRGCSKLKLLGKIVWRSFLLICIGVIIVNPNYCLGPLSWDKVRI
                     PGVLQRLGVTYFVVAVLEFFFWKPVPDSCTLESSCFSLRDITSSWPQWLTILTLESIW
                     LALTFFLPVPGCPTGYLGPGGIGDLGKYPHCTGGAAGYIDRLLLGDNHLYQHPSSTVL
                     YHTEVAYDPEGVLGTINSIVMAFLGVQAGKILVYYKDQTKAILTRFAAWCCILGLISI
                     VLTKVSANEGFIPINKNLWSISYVTTLSCFAFFILLILYPVVDVKGLWTGTPFFYPGM
                     NSILVYVGHEVLENYFPFQWKLADEQSHKEHLIQNIVATALWVLIAYVLYKKKLFWKI
                     "
     exon            153..268
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            269..405
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            406..527
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            528..597
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            598..667
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            668..771
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            772..848
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            849..879
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            880..1040
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1156
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1157..1278
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1405
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1492
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..1570
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1641
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1754
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     exon            1755..4603
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2407..2412
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /note="hexamer: TATAAA"
     polyA_site      2426
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /note="major polyA site"
     regulatory      4581..4586
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
                     /note="hexamer: AATAAA"
     polyA_site      4603
                     /gene="Hgsnat"
                     /gene_synonym="9430010M12Rik; D8Ertd354e; Tmem76"
ORIGIN      
        1 agaagagcgc agctcggctg cggggacaga gcgcaactcc cggagagagg ctgtgggagg
       61 catgggcgcg ggcccggcgc tggcagcgct gctgctggcc gggtcggtgc tgagcgccac
      121 gctgctggcc cccggccgcc gggcggagcc agatttagat gaaaaaagga acgtggagct
      181 gaagatggat caggctttgc tgcttattca caatgaactc ttggggacga gcctgactgt
      241 gtactggaaa tctgatgact gttaccaatg caccttccag ccactggcga acgtctctca
      301 cggtggaaag ccagcaaagc caagcgtggc acctgtctct gtgagcaccc agcatgggtc
      361 catcctgcag gttaactcca cctcggagga gagagcagct tgcaggctgg aatacaaatt
      421 tggagaattt ggaaactatt cccttttggt gcagcatgct tccagcgggg ccaataaaat
      481 tgcctgtgac atcatcgtca atgagaatcc agtggacagt aaccttcctg tgagcatcgc
      541 attccttgtt ggtttagccc tcatcgtcgc agtatccctc ctgaggctct tgctgagttt
      601 ggatgatgtc aataactgga tatctaagac aatagcttct cgagaaactg accgtctcat
      661 caattctgag ctagggtctc ccagcagagc agaccctctc agcgctgatt accaaccaga
      721 aactcggcgt tcttctgcga accgtctccg atgtgtggac acatttaggg ggttagctct
      781 cgtcctcatg gtctttgtta actatggagg aggaaagtat tggtacttca aacattcaag
      841 ttggaacgga ctgactgtgg ccgacctcgt cttcccatgg tttgtattta ttatgggaac
      901 ttcaattttc ctatcaatga cttctatcct gcaacgagga tgttcaaaac tcaaactgct
      961 ggggaagatt gtgtggagaa gcttcctgct aatctgtata ggagttatca ttgtcaaccc
     1021 caattattgc cttggtccat tgtcttggga taaagtgcgg atccctggtg tcctgcagcg
     1081 gttgggagtg acatacttcg tggtcgctgt gttggagttc tttttctgga agcctgtgcc
     1141 tgacagttgt accttggaaa gcagctgctt ctctctccga gacatcacgt ccagctggcc
     1201 ccagtggctc accattctga cgctggaaag catttggctg gctttgacat tctttctgcc
     1261 tgtccctggg tgccctactg gctaccttgg ccctggcggt ataggggact tggggaagta
     1321 tcctcattgt actggaggag cagctggcta catcgaccgc ctgctcctgg gagacaatca
     1381 cctgtaccag cacccatcct ccaccgtgct gtatcacaca gaggtggcct acgaccctga
     1441 aggagtctta gggactatca actcaattgt gatggcgttt ttaggagttc aggcaggaaa
     1501 aatactggtg tattacaagg atcagacgaa agccatcctg acccgatttg ccgcctggtg
     1561 ctgtatcctt gggcttattt ctattgtgtt gacaaaagtg tctgcaaatg aaggttttat
     1621 tccaataaac aagaatctct ggtccatctc gtacgtcacc acactgagct gctttgcctt
     1681 cttcatcctc ctcattctct acccagttgt ggatgtgaag ggcctgtgga cgggaacccc
     1741 cttcttctat ccagggatga actccatcct ggtgtatgtt ggccacgagg tgttggagaa
     1801 ctacttcccc ttccagtgga agctggcgga tgagcagtca cacaaagagc atctcattca
     1861 gaacatagtc gccacggctc tctgggtgct catcgcctac gtgctctata aaaagaagct
     1921 gttttggaaa atctgacagc acccgctgaa atacatagct agaggagtcc agggggtctg
     1981 ggagaagagc ggcggcagcc tccctcacag aggggattgc ttccagatgc tgcgtccaaa
     2041 ccgatttgct tagacatcag aaagttgcct gtgtctttct gactcacata tctatgcatc
     2101 tgaaatgcca tgctctccgc tttctccatt ttgtatgaaa ttgctatatt tggaacatgt
     2161 gtggaaatct tgtttaactt ctcagagatt tactctggac attttgcctc tgtgaaagag
     2221 gccaaggttg gtagactgtg cttgattcag agagaaccca ttccaagctt ggacagcacc
     2281 tggggtttgg tccctggcat cacaaacaag caaacaacaa aaagaaccaa gtcttatttc
     2341 ctgaaaactg actttccacg tgtatgcgca tgccgaccgt accgccgtgg tttcatggtc
     2401 acagactata aagctttttg aatggactgt gtcatgttgt ctgattttat ttattttttt
     2461 ttttatagta tgaactttat tgtctctaga aagccaaagt gattttcctt gtccactttc
     2521 acggctttga tggccccacc aaatgcatca tttaaagcta ctcgcttctt tcctcacttg
     2581 gaagaaagct gaaatataga tttgttattg caaaccactc tcgttacagc tctgtctgtc
     2641 catctacctt attattgtct gtctgtctgt ctgtgctggc atgtctctgg atgataccta
     2701 ggcatctcta ggtacccaga gatggagccc tgggaagaag aacgttcctc cggagctgtc
     2761 tcctgggtaa ggtgtggggc catcccctgg cctgtcagag ctttggttcc ctcatctgtg
     2821 gaaggaaagg aacagggatg ctccctcctc tctcagggtt tggatgtgat aaggcgggac
     2881 ccaggagccg cttctgtgcc atctcacaga aaaccctggg aaaccaatca tcaaaaaacc
     2941 tctctgtctg catccaagtg aaccctacca atggctgtct gtggtttctt agggatgaaa
     3001 gcgatgactg tgtgtacaga cgccctcctg ggaatttccc aggagaattg tttttttgtt
     3061 ttttttttgt tttgtttttt ttttttttgg tttttctaga cagggtttct ctgtgtagcc
     3121 ctggctgtcc tggaactcac tttgtagacc aggctgacct cgaactcaga aatctgcctg
     3181 cctctgcctt ccaagtgctg ggattaaagg catgtgccac catgcccgat gagaattgca
     3241 ttttaaagaa gcagcacgat ggggtttgtc ttagtcagag tttctattct gcacaaatat
     3301 catgaccaag aagcagttgg ggaggaaagg gtttattcag cttatacttc catactgctg
     3361 ttcatcacca aaggaagtca ggactggaac tcaagcaggt cagaaagcag gagctgatgc
     3421 agaggccatg gagggatgtt cttcactggc ttgcttcccc tggcttgctc agcctgctct
     3481 cttatagaac caagactacc agcccagaga tggtcccacc cacaaggggc ctttccccct
     3541 tgatcactaa ttgagaaaat gccttacagt tggatctcat ggaggcattt cctcaactga
     3601 agctcctttc tctgtgataa ccgcagcctg tgtcaagttg acacacaaaa ctagccagta
     3661 cagggttgta cttcagacga tccttctgtg gtgacctggg gacattagaa tcatgtgcga
     3721 atggcttttg agtcagtccc ctgctctgcc ctgggctctt ggctgggctt cctgttgtct
     3781 tctctcctat ggccaaagca tctctggccc agctcctccc ttctctacaa atggaaactc
     3841 cttcatgttt tcctagttct aaatgattca ttctctcaga gcactgagct aactgcccct
     3901 gtcttttcac tacttaactt ggactttccc atgaaaaaaa attttataca tgtacatata
     3961 tatatgtatg tatgtatgta tgtatgtatg tatgtatata tacacacaca cacatatata
     4021 caccatatat gtcatatata tgtatatatg tcattgctaa gtcttgtcag ttccattccc
     4081 atctcccacc tctttgttct ctatatggcc tctgttctgt gtagctgcct ctttctgtga
     4141 agtcactggt ccactgccac cagatccagc tttatgaagc aggagttgga tcaaaccatc
     4201 ttgtccaatt cggttgtgcc tggggcatct ctgcctgcca cagcccactt cactgatccc
     4261 agcacctgct gttttcctgt ctccgccagc cttgctggct gtagctcaga tggacccctc
     4321 cctccttcct cctcatgtct ccccacctgt atttgatctg tcgttcacag ggcccattgt
     4381 ggaagcatca cctctaatct cattctcggc cagagctgat ctcctcttcg gggttctcac
     4441 tgacacccat ctcctagcat taatcgcttc tgcctcgtga gatgattatt ttctctgtta
     4501 tcgtgatgtc aatggcaaga attacatatt ctgtattttt tgtggtacca aatgcactgc
     4561 cttgtactta gcaagtgcta aataaacatt tattaaagca aaa
//
