LOCUS       NM_008244               2905 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus HGF-regulated tyrosine kinase substrate (Hgs),
            transcript variant 2, mRNA.
ACCESSION   NM_008244
VERSION     NM_008244.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Hermosilla Aguayo,V., Martin,P., Tian,N., Zheng,J., Aho,R., Losa,M.
            and Selleri,L.
  TITLE     ESCRT-dependent control of craniofacial morphogenesis with
            concomitant perturbation of NOTCH signaling
  JOURNAL   Dev Biol 503, 25-42 (2023)
   PUBMED   37573008
REFERENCE   3  (bases 1 to 2905)
  AUTHORS   McLean,J.W., Wilson,J.A., Tian,T., Watson,J.A., VanHart,M.,
            Bean,A.J., Scherer,S.S., Crossman,D.K., Ubogu,E. and Wilson,S.M.
  TITLE     Disruption of Endosomal Sorting in Schwann Cells Leads to Defective
            Myelination and Endosomal Abnormalities Observed in
            Charcot-Marie-Tooth Disease
  JOURNAL   J Neurosci 42 (25), 5085-5101 (2022)
   PUBMED   35589390
REFERENCE   4  (bases 1 to 2905)
  AUTHORS   Birdsall,V., Kirwan,K., Zhu,M., Imoto,Y., Wilson,S.M., Watanabe,S.
            and Waites,C.L.
  TITLE     Axonal transport of Hrs is activity dependent and facilitates
            synaptic vesicle protein degradation
  JOURNAL   Life Sci Alliance 5 (10), e202000745 (2022)
   PUBMED   35636965
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2905)
  AUTHORS   Li,Z., Wang,T., Xin,C., Song,Y., Kong,J., Xu,J., Liu,Q., Teng,Y.,
            Hou,N., Cheng,X., Yang,G., Liu,W., Zhou,B., Zhang,Y., Yang,X. and
            Wang,J.
  TITLE     Hgs Deficiency Caused Restrictive Cardiomyopathy via Disrupting
            Proteostasis
  JOURNAL   Int J Biol Sci 18 (5), 2018-2031 (2022)
   PUBMED   35342336
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2905)
  AUTHORS   Lu,L., Komada,M. and Kitamura,N.
  TITLE     Human Hrs, a tyrosine kinase substrate in growth factor-stimulated
            cells: cDNA cloning and mapping of the gene to chromosome 17
  JOURNAL   Gene 213 (1-2), 125-132 (1998)
   PUBMED   9630564
REFERENCE   7  (bases 1 to 2905)
  AUTHORS   Asao,H., Sasaki,Y., Arita,T., Tanaka,N., Endo,K., Kasai,H.,
            Takeshita,T., Endo,Y., Fujita,T. and Sugamura,K.
  TITLE     Hrs is associated with STAM, a signal-transducing adaptor molecule.
            Its suppressive effect on cytokine-induced cell growth
  JOURNAL   J Biol Chem 272 (52), 32785-32791 (1997)
   PUBMED   9407053
REFERENCE   8  (bases 1 to 2905)
  AUTHORS   Komada,M. and Kitamura,N.
  TITLE     Growth factor-induced tyrosine phosphorylation of Hrs, a novel
            115-kilodalton protein with a structurally conserved putative zinc
            finger domain
  JOURNAL   Mol Cell Biol 15 (11), 6213-6221 (1995)
   PUBMED   7565774
REFERENCE   9  (bases 1 to 2905)
  AUTHORS   Kelton,D.E. and Rauch,H.
  TITLE     Linkage of open eyelids with linkage group VII of the mouse
  JOURNAL   J Hered 59 (1), 27-28 (1968)
   PUBMED   5690495
REFERENCE   10 (bases 1 to 2905)
  AUTHORS   Meier,H.
  TITLE     The neuropathy of teetering, a neurological mutation in the mouse
  JOURNAL   Arch Neurol 16 (1), 59-66 (1967)
   PUBMED   6024256
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY315543.1, AK046299.1, AK145031.1 and CJ294093.1.
            
            On Apr 16, 2009 this sequence version replaced NM_008244.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site, compared to variant 1. This difference results in a
            protein (isoform 2) that is one amino acid shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC003239.1, AK046299.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-40                BY315543.1         1-40
            41-1841             AK046299.1         2-1802
            1842-2041           AK145031.1         1765-1964
            2042-2894           AK046299.1         2003-2855
            2895-2905           CJ294093.1         335-345
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 84.16 cM"
     gene            1..2905
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /note="HGF-regulated tyrosine kinase substrate"
                     /db_xref="GeneID:15239"
                     /db_xref="MGI:MGI:104681"
     exon            1..129
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..2420
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /note="isoform 2 is encoded by transcript variant 2;
                     hepatocyte growth factor-regulated tyrosine kinase
                     substrate"
                     /codon_start=1
                     /product="hepatocyte growth factor-regulated tyrosine
                     kinase substrate isoform 2"
                     /protein_id="NP_032270.3"
                     /db_xref="CCDS:CCDS88297.1"
                     /db_xref="GeneID:15239"
                     /db_xref="MGI:MGI:104681"
                     /translation="MGRGSGTFERLLDKATSQLLLETDWESILQICDLIRQGDTQAKY
                     AVNSIKKKVNDKNPHVALYALEVMESVVKNCGQTVHDEVANKQTMEELKELLKRQVEV
                     NVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGHVFPEFKESDAMFAAERAPDW
                     VDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVRVCEPCYEQ
                     LNKKAEGKASSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEE
                     KERMRQKTTYTAHPKAEPTPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNY
                     WEKKQEEARKSPTPSAPVPLTEPAAQPGEGHTAPNSMAEAPLPETDSQPITPCSGPFS
                     EYQNGESEESHEQFLKALQNAVSTFVNRMKSNHMRGRSITNDSAVLSLFQSINTMHPQ
                     LLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHREKLRRAAEEAERQRQIQL
                     AQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPAFPLPYA
                     QLQAMPTAGGVLYQPSGPTSFPATFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTT
                     ADPSMVSAYMYPTGAPGAQAAPQAQAGPTTSPAYSSYQPTPTPGYQSVASQAPQSLPA
                     ISQPPQTSNIGYMGSQPMSMGYQPYNMQNLMTALPGQDASLPAQQPYIPGQQPLYQQM
                     APSTGPPQQQPPVAQPAPTQGPPAQGSEAQLISFD"
     exon            130..214
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            215..290
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            291..383
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            384..507
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            508..560
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            561..629
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            630..754
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            755..833
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            834..932
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            933..1028
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1029..1067
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1211
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1271
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1479
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..1652
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1793
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1968
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..2102
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2222
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            2223..2309
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     exon            2310..2905
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2882..2887
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /note="hexamer: AATAAA"
     polyA_site      2900
                     /gene="Hgs"
                     /gene_synonym="Hgr; Hrs; tn; ZFYVE8"
                     /note="major polyA site"
ORIGIN      
        1 gagcagaaaa agaaaggcgg aagcggaagt tggggggcgc gtcagctccg aaagagggag
       61 cgactgcagc gtcggtccgg agtggggtcg ccatggggcg aggcagcggc accttcgagc
      121 gtctcctaga caaagccacc agccagcttc tgttggagac agactgggag tccattctac
      181 agatctgcga cctgatccgt cagggggaca cacaagcaaa atatgctgta aactccatca
      241 agaagaaggt taatgataag aacccacacg tggctttgta tgctctggag gtgatggagt
      301 ctgtggtaaa gaactgtggc cagacagtcc atgatgaagt ggccaacaaa cagaccatgg
      361 aagaactgaa ggagctgctg aagaggcaag tggaagttaa tgttcggaac aagatcttgt
      421 acctgatcca ggcctgggca catgccttcc ggaacgaacc caagtacaag gtggtccagg
      481 acacatacca gatcatgaag gtagaaggac atgtcttccc tgaatttaag gaaagtgatg
      541 ccatgttcgc tgctgaaaga gcccctgact gggtggatgc tgaggaatgc catcggtgca
      601 gagtacagtt tggggtggtg acccgcaagc atcactgccg agcatgtggg cagatcttct
      661 gtggcaagtg ctcctccaag tactccacca tccccaagtt cggcattgag aaggaggtgc
      721 gcgtgtgtga gccctgctat gagcagctga acaagaaggc agaagggaag gcttcctcta
      781 ccactgagct gcccccagag tacctgacca gccccctgtc acagcagtct cagctgcccc
      841 caaagcggga tgagacagcc ctgcaggaag aggaggagct acagctggct ctggccctat
      901 cacagtcaga ggctgaggag aaagagagga tgagacagaa aacaacatat acagcacatc
      961 caaaggcaga gcccacgccc ttggcttcct ctgcgccccc agctggcagc ctgtattcct
     1021 cgcctgtgaa ctcatcagca cctctggctg aggacatcga ccctgagctt gcaagatacc
     1081 tcaaccggaa ctactgggag aagaaacagg aggaagcacg gaagagcccc acaccatctg
     1141 cacctgtgcc cctgacagag cctgctgccc agcctggaga aggacataca gcccccaaca
     1201 gcatggcaga ggctcctctt ccagagacag actctcagcc cataactccc tgcagcggcc
     1261 cctttagtga gtaccagaat ggggagtcgg aggagagcca tgagcagttc ctcaaggccc
     1321 tgcagaatgc cgtgagcact tttgtcaacc gcatgaagag caaccacatg cggggacgca
     1381 gcatcaccaa cgactcggct gtgctgtcac tcttccagtc catcaacacc atgcacccgc
     1441 agctgctcga gctgctcaac cagctggatg agcgcaggct gtactacgag ggccttcagg
     1501 acaagctggc acagatacgt gacgcccgag gggctctgag cgccctgcgt gaagaacaca
     1561 gggagaagct gcgccgggca gctgaggagg ctgagcgtca acgccagatc cagctggcac
     1621 agaaactgga gatcatgaga cagaagaagc aggagtacct ggaggtgcag agacagctag
     1681 ctatccagcg cctgcaggaa caggagaagg aacggcagat gcgtctggag caacagaagc
     1741 agactgtcca gatgcgcgcc cagatgcctg cctttccctt gccttatgcc cagctccagg
     1801 ctatgcccac ggctgggggt gtactctacc agccctcagg cccaaccagc ttccctgcca
     1861 ccttcagccc agcaggctca gtagagggct ctccgatgca tggtgtgtat atgagccagc
     1921 cagccccagc cactggcccc taccccagca tgcctggcac aacagcagat cccagcatgg
     1981 tcagcgccta catgtaccca acaggtgccc ctggggcaca ggcagcccct caggcccagg
     2041 ccgggcccac caccagtcct gcctactcct cctaccagcc cacaccaacc ccaggctacc
     2101 agagcgtggc ttctcaggcc ccacagagcc tcccagccat ctcacagcct ccacagacca
     2161 gcaacatagg ctacatgggg agccagccaa tgtccatggg ctaccagccg tacaatatgc
     2221 agaatctcat gaccgccctt cccgggcagg atgcgtctct gccagcccag cagccctaca
     2281 tcccagggca gcagcccctg taccagcaga tggcccccag caccggccct ccccagcagc
     2341 aaccccctgt ggcccagcca gcgcctacac agggaccgcc agcacagggc agtgaggccc
     2401 agctcatctc ctttgactga ccttgagtct ggcgctcacc atgcagagta acactacagt
     2461 tcaccagaaa ccacttatat gtctaactag ccattcctcc cctattactg ccctgtagtg
     2521 tcccttctgt gagcaagggg tgggccttcc cccttggccc tcttccctgt cctcggtggt
     2581 ctggctccta tcactggttc cctgcttcag tcctgatgcc gtctgacctc cccgggactg
     2641 gggctctgca gggcaagtag aaccttctct ggagagtgcc cggctgtgtt gggtgccaca
     2701 ggtgaatgct gtggggatgc ttagcctggc ccggagtctg atgtgaacta actgtgtggt
     2761 gtccaggcct ggccctggta cccagaagaa ccatgtggct cctgcacagc aagggtagaa
     2821 gctttaggta tctctgtctc cccttctcct tggatgtcat ctctcagtgc agagcagtga
     2881 aaataaaatg tatttcaaaa ctctc
//
