LOCUS       NM_001289461            2645 bp    mRNA    linear   ROD 24-APR-2024
DEFINITION  Mus musculus hepatocyte growth factor (Hgf), transcript variant 5,
            mRNA.
ACCESSION   NM_001289461 XM_006535630
VERSION     NM_001289461.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ogata,K., Moriyama,M., Kawado,T., Yoshioka,H., Yano,A.,
            Matsumura-Kawashima,M., Nakamura,S. and Kawano,S.
  TITLE     Extracellular vesicles of iPS cells highly capable of producing HGF
            and TGF-beta1 can attenuate Sjogren's syndrome via innate immunity
            regulation
  JOURNAL   Cell Signal 113, 110980 (2024)
   PUBMED   37981065
  REMARK    GeneRIF: Extracellular vesicles of iPS cells highly capable of
            producing HGF and TGF-beta1 can attenuate Sjogren's syndrome via
            innate immunity regulation.
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Li,Y., Li,X., Yang,Y., Li,F., Chen,Q., Zhao,Z., Zhang,N. and Li,H.
  TITLE     Hepatocyte growth factor attenuates high glucose-disturbed
            mitochondrial dynamics in podocytes by decreasing ARF6-dependent
            DRP1 translocation
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (1), 119623 (2024)
   PUBMED   37913847
  REMARK    GeneRIF: Hepatocyte growth factor attenuates high glucose-disturbed
            mitochondrial dynamics in podocytes by decreasing ARF6-dependent
            DRP1 translocation.
REFERENCE   3  (bases 1 to 2645)
  AUTHORS   Peng,Z., Lv,X., Zhang,P., Chen,Q., Zhang,H., Chen,J., Ma,X.,
            Ouyang,B., Hao,M., Tong,H., Guo,D., Luo,Y. and Huang,S.
  TITLE     Intercellular Interactions Mediated by HGF And TGF-Beta Promote the
            3D Spherical and Xenograft Growth of Liver Cancer Cells
  JOURNAL   Curr Protein Pept Sci 25 (1), 71-82 (2024)
   PUBMED   37642183
  REMARK    GeneRIF: Intercellular Interactions Mediated by HGF And TGF-Beta
            Promote the 3D Spherical and Xenograft Growth of Liver Cancer
            Cells.
REFERENCE   4  (bases 1 to 2645)
  AUTHORS   Fernandes,M., Hoggard,B., Jamme,P., Paget,S., Truong,M.J.,
            Gregoire,V., Vinchent,A., Descarpentries,C., Morabito,A.,
            Stanislovas,J., Farage,E., Meneboo,J.P., Sebda,S.,
            Bouchekioua-Bouzaghou,K., Nollet,M., Humez,S., Perera,T.,
            Fromme,P., Grumolato,L., Figeac,M., Copin,M.C., Tulasne,D.,
            Cortot,A.B., Kermorgant,S. and Kherrouche,Z.
  TITLE     MET exon 14 skipping mutation is a hepatocyte growth factor
            (HGF)-dependent oncogenic driver in vitro and in humanised HGF
            knock-in mice
  JOURNAL   Mol Oncol 17 (11), 2257-2274 (2023)
   PUBMED   36799689
  REMARK    GeneRIF: MET exon 14 skipping mutation is a hepatocyte growth
            factor (HGF)-dependent oncogenic driver in vitro and in humanised
            HGF knock-in mice.
REFERENCE   5  (bases 1 to 2645)
  AUTHORS   Du,J., Jiang,Y., Liu,X., Ji,X., Xu,B., Zhang,Y., Liu,Y., Zhang,T.
            and Lin,J.
  TITLE     HGF Secreted by Menstrual Blood-Derived Endometrial Stem Cells
            Ameliorates Non-Alcoholic Fatty Liver Disease Through
            Downregulation of Hepatic Rnf186
  JOURNAL   Stem Cells 41 (2), 153-168 (2023)
   PUBMED   36573461
  REMARK    GeneRIF: HGF Secreted by Menstrual Blood-Derived Endometrial Stem
            Cells Ameliorates Non-Alcoholic Fatty Liver Disease Through
            Downregulation of Hepatic Rnf186.
REFERENCE   6  (bases 1 to 2645)
  AUTHORS   Nakamura,T., Sakai,K., Nakamura,T. and Matsumoto,K.
  TITLE     Hepatocyte growth factor twenty years on: Much more than a growth
            factor
  JOURNAL   J Gastroenterol Hepatol 26 Suppl 1, 188-202 (2011)
   PUBMED   21199531
  REMARK    Review article
REFERENCE   7  (bases 1 to 2645)
  AUTHORS   Rong,S., Bodescot,M., Blair,D., Dunn,J., Nakamura,T., Mizuno,K.,
            Park,M., Chan,A., Aaronson,S. and Vande Woude,G.F.
  TITLE     Tumorigenicity of the met proto-oncogene and the gene for
            hepatocyte growth factor
  JOURNAL   Mol Cell Biol 12 (11), 5152-5158 (1992)
   PUBMED   1406687
REFERENCE   8  (bases 1 to 2645)
  AUTHORS   Chatani,Y., Itoh,A., Tanaka,E., Hattori,A., Nakamura,T. and
            Kohno,M.
  TITLE     Hepatocyte growth factor rapidly induces the tyrosine
            phosphorylation of 41-kDa and 43-kDa proteins in mouse
            keratinocytes
  JOURNAL   Biochem Biophys Res Commun 185 (3), 860-866 (1992)
   PUBMED   1385703
REFERENCE   9  (bases 1 to 2645)
  AUTHORS   Szpirer,C., Riviere,M., Cortese,R., Nakamura,T., Islam,M.Q.,
            Levan,G. and Szpirer,J.
  TITLE     Chromosomal localization in man and rat of the genes encoding the
            liver-enriched transcription factors C/EBP, DBP, and HNF1/LFB-1
            (CEBP, DBP, and transcription factor 1, TCF1, respectively) and of
            the hepatocyte growth factor/scatter factor gene (HGF)
  JOURNAL   Genomics 13 (2), 293-300 (1992)
   PUBMED   1535333
REFERENCE   10 (bases 1 to 2645)
  AUTHORS   Coffer,A., Fellows,J., Young,S., Pappin,D. and Rahman,D.
  TITLE     Purification and characterization of biologically active scatter
            factor from ras-transformed NIH 3T3 conditioned medium
  JOURNAL   Biochem J 278 (Pt 1) (Pt 1), 35-41 (1991)
   PUBMED   1831975
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC161176.5.
            
            On Jan 22, 2014 this sequence version replaced XM_006535630.1.
            
            Summary: This gene encodes a protein that binds to the hepatocyte
            growth factor receptor to regulate cell growth, cell motility and
            morphogenesis in numerous cell and tissue types. The encoded
            preproprotein is proteolytically processed to generate multiple
            protein products, including the hepatocyte growth factor alpha and
            beta chains, which heterodimerize to form the mature active
            protein. Although this protein is a member of the peptidase S1
            family of serine proteases, it lacks peptidase activity. Homozygous
            knockout mice for this gene exhibit embryonic lethality due to
            impaired development of the placenta and liver. Alternative
            splicing results in multiple transcript variants encoding different
            isoforms. [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (5) differs in the 5' UTR and uses
            an alternate in-frame splice site, compared to variant 1. The
            encoded isoform (3) is shorter than isoform 1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: D10213.1, SRR13948564.4233359.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               AC161176.5         54260-54377
            119-284             AC161176.5         61318-61483
            285-397             AC161176.5         65146-65258
            398-512             AC161176.5         67106-67220
            513-640             AC161176.5         72866-72993
            641-761             AC161176.5         77268-77388
            762-880             AC161176.5         78802-78920
            881-1055            AC161176.5         94203-94377
            1056-1183           AC161176.5         98494-98621
            1184-1286           AC161176.5         102642-102744
            1287-1420           AC161176.5         105147-105280
            1421-1459           AC161176.5         110465-110503
            1460-1556           AC161176.5         112179-112275
            1557-1631           AC161176.5         114115-114189
            1632-1778           AC161176.5         115251-115397
            1779-1885           AC161176.5         115863-115969
            1886-2031           AC161176.5         116089-116234
            2032-2645           AC161176.5         119185-119798
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 7.07 cM"
     gene            1..2645
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /note="hepatocyte growth factor"
                     /db_xref="GeneID:15234"
                     /db_xref="MGI:MGI:96079"
     exon            1..118
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     CDS             28..2199
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /note="isoform 3 preproprotein is encoded by transcript
                     variant 5; scatter factor; hepatopoietin-A"
                     /codon_start=1
                     /product="hepatocyte growth factor isoform 3
                     preproprotein"
                     /protein_id="NP_001276390.1"
                     /db_xref="CCDS:CCDS80213.1"
                     /db_xref="GeneID:15234"
                     /db_xref="MGI:MGI:96079"
                     /translation="MMWGTKLLPVLLLQHVLLHLLLLHVAIPYAEGQKKRRNTLHEFK
                     KSAKTTLTKEDPLLKIKTKKVNSADECANRCIRNRGFTFTCKAFVFDKSRKRCYWYPF
                     NSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHE
                     HSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGP
                     MDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPW
                     EYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHD
                     ITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNG
                     KNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYT
                     GNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLK
                     YRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNIS
                     QLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINAD
                     GLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKM
                     RMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL"
     exon            119..284
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            285..397
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            398..512
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            513..640
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            641..761
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            762..880
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            881..1055
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1183
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1184..1286
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1287..1420
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1459
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1556
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1631
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1778
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1885
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..2031
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     exon            2032..2645
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2333..2338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /note="hexamer: AATATA"
     polyA_site      2356
                     /gene="Hgf"
                     /gene_synonym="C230052L06Rik; HGF/SF; NK1; NK2; SF;
                     SF/HGF"
                     /note="major polyA site"
ORIGIN      
        1 agggatcagt ctgctcgaac tgcaagcatg atgtggggga ccaaacttct gccggtcctg
       61 ttgctgcagc atgtcctcct gcacctcctc ctgcttcatg tcgccatccc ctatgcagaa
      121 ggacagaaga aaagaagaaa tacacttcat gaatttaaaa agtcagcaaa aactactctt
      181 accaaggaag acccattact gaagattaaa accaaaaaag tgaactctgc agatgagtgt
      241 gccaacaggt gtatcaggaa caggggcttt acgttcactt gcaaggcctt cgtttttgat
      301 aagtcaagaa aacgatgcta ctggtatcct ttcaatagta tgtcaagtgg agtgaaaaaa
      361 gggtttggcc atgaatttga cctctatgaa aacaaagact atattagaaa ctgcatcatt
      421 ggtaaaggag gcagctataa agggacggta tccatcacta agagtggcat caaatgccag
      481 ccttggaatt ccatgatccc ccatgaacac agctatcgcg gtaaagacct acaggaaaac
      541 tactgtcgaa atcctcgagg ggaagaaggg ggaccctggt gtttcacaag caatccagag
      601 gtacgctacg aagtctgtga cattcctcag tgttcagaag ttgaatgcat gacctgcaat
      661 ggtgaaagct acagaggtcc catggatcac acagaatcag gcaagacttg tcagcgctgg
      721 gaccagcaga caccacaccg gcacaagttc ttgccagaaa gatatcccga caagggcttt
      781 gatgataatt attgccgcaa tcctgatggc aagccgaggc catggtgcta cactcttgac
      841 cctgacaccc cttgggagta ttgtgcaatt aaaacgtgcg ctcacagtgc tgtgaatgag
      901 actgatgtcc ctatggaaac aactgaatgc attcaaggcc aaggagaagg ttacagggga
      961 accagcaata ccatttggaa tggaattccc tgtcagcgtt gggattcgca gtaccctcac
     1021 aagcatgata tcactcccga gaacttcaaa tgcaaggacc ttagagaaaa ttattgccgc
     1081 aatccagatg gggctgaatc accatggtgt tttaccactg acccaaacat ccgagttggc
     1141 tactgctctc aaattcccaa gtgtgacgtg tcaagtggac aagattgtta tcgtggcaat
     1201 gggaaaaatt acatgggcaa cttatccaaa acaaggtctg gacttacatg ttccatgtgg
     1261 gacaagaata tggaggattt acaccgtcat atcttctggg agccagatgc tagcaaattg
     1321 aataagaatt actgccggaa tcctgatgat gatgcccatg gaccttggtg ctacacgggg
     1381 aatcctctta ttccttggga ttattgccct atttcccgtt gtgaaggaga tactacacct
     1441 acaattgtca atttggacca tcctgtaata tcctgtgcca aaacaaaaca actgcgggtt
     1501 gtaaatggca ttccaacaca aacaacagta gggtggatgg ttagtttgaa atacagaaat
     1561 aaacatatct gtggaggatc attgataaag gaaagttggg ttcttactgc aagacaatgt
     1621 tttccagcca gaaacaaaga cttgaaagac tatgaagctt ggcttggcat ccacgatgtt
     1681 catgagagag gcgaggagaa gcgcaagcag atcttaaaca tttcccagct ggtctatggt
     1741 cctgaaggct cagacttggt tttactgaag cttgctcgac ctgcaatcct ggataacttt
     1801 gtcagtacaa ttgatttacc tagttatggt tgtacaatcc ctgaaaagac cacttgcagt
     1861 atttacggct ggggctacac tggattgatc aacgcggatg gtttattacg agtagctcat
     1921 ctgtatatta tggggaatga gaaatgcagt cagcaccatc aaggcaaggt gactttgaat
     1981 gagtctgagt tatgtgctgg ggctgaaaag attggatcag gaccatgtga gggagattat
     2041 ggtggcccac tcatttgtga acaacacaaa atgagaatgg ttcttggtgt cattgttcct
     2101 ggtcgtggat gtgccatccc aaatcgtcct ggtatttttg ttcgagtagc atattatgca
     2161 aaatggatac acaaagtaat tttgacatac aagttgtaat agccatagaa gaggccagtg
     2221 tgtttgaagc atccatggat acatgaggat ttccaagact tcaggatcaa aatgtcacct
     2281 aaaacaatcc tagaacaact acttgagtgt tgtgagtgtt cagatactca ttaatatata
     2341 tgggtgtttt ctgttgtatt gtttgtcagt gtcctttgta aatgttcaag tgaattaagg
     2401 tacctgcaag tgcagtaaca tatctcctga agatacttga atgaattaaa aaaatataaa
     2461 tttagagttg ctagatgcta aagttttaaa gaaaaaggtt aattaatcac tctatgctgc
     2521 tttccaggtt aatatcttag tgatgaataa attgtatgtt aaacattatt ttaaccccac
     2581 caaaacaatt tatacattgt ttttaaattg taagtctata ttaaattata ttacatttca
     2641 tatgc
//
