LOCUS       NM_177873               4989 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1),
            transcript variant 1, mRNA.
ACCESSION   NM_177873 XM_284117
VERSION     NM_177873.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ito,M., Yun,Y., Kulkarni,D.S., Lee,S., Sandhu,S., Nunez,B., Hu,L.,
            Lee,K., Lim,N., Hirota,R.M., Prendergast,R., Huang,C., Huang,I. and
            Hunter,N.
  TITLE     Distinct and interdependent functions of three RING proteins
            regulate recombination during mammalian meiosis
  JOURNAL   Proc Natl Acad Sci U S A 122 (2), e2412961121 (2025)
   PUBMED   39761402
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   He,Y., Wang,H., Hong,T., Hu,L., Gao,C., Gao,L., Cui,Y., Tan,R.,
            Pu,D. and Wu,J.
  TITLE     HFM1 is essential for the germ cell intercellular bridge transport
            in primordial follicle formation in mice
  JOURNAL   Cell Mol Life Sci 82 (1), 28 (2024)
   PUBMED   39725823
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4989)
  AUTHORS   Rezaei,M., Liang,M., Yalcin,Z., Martin,J.H., Kazemi,P., Bareke,E.,
            Ge,Z.J., Fardaei,M., Benadiva,C., Hemida,R., Hassan,A., Maher,G.J.,
            Abdalla,E., Buckett,W., Bolze,P.A., Sandhu,I., Duman,O.,
            Agrawal,S., Qian,J., Vallian Broojeni,J., Bhati,L., Miron,P.,
            Allias,F., Selim,A., Fisher,R.A., Seckl,M.J., Sauthier,P.,
            Touitou,I., Tan,S.L., Majewski,J., Taketo,T. and Slim,R.
  TITLE     Defects in meiosis I contribute to the genesis of androgenetic
            hydatidiform moles
  JOURNAL   J Clin Invest 134 (22), e170669 (2024)
   PUBMED   39545410
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4989)
  AUTHORS   Zhong,C., Wang,H., Yuan,X., He,Y., Cong,J., Yang,R., Ma,W., Gao,L.,
            Gao,C., Cui,Y., Wu,J., Tan,R. and Pu,D.
  TITLE     The crucial role of HFM1 in regulating FUS ubiquitination and
            localization for oocyte meiosis prophase I progression in mice
  JOURNAL   Biol Res 57 (1), 36 (2024)
   PUBMED   38822414
  REMARK    GeneRIF: The crucial role of HFM1 in regulating FUS ubiquitination
            and localization for oocyte meiosis prophase I progression in mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4989)
  AUTHORS   Xie,X., Murtaza,G., Li,Y., Zhou,J., Ye,J., Khan,R., Jiang,L.,
            Khan,I., Zubair,M., Yin,H., Jiang,H., Liu,W., Shi,B., Hou,X.,
            Gong,C., Fan,S., Wang,Y., Jiang,X., Zhang,Y., Zhang,H., Ma,H. and
            Shi,Q.
  TITLE     Biallelic HFM1 variants cause non-obstructive azoospermia with
            meiotic arrest in humans by impairing crossover formation to
            varying degrees
  JOURNAL   Hum Reprod 37 (7), 1664-1677 (2022)
   PUBMED   35526155
  REMARK    GeneRIF: Biallelic HFM1 variants cause non-obstructive azoospermia
            with meiotic arrest in humans by impairing crossover formation to
            varying degrees.
REFERENCE   6  (bases 1 to 4989)
  AUTHORS   Previato de Almeida,L., Evatt,J.M., Chuong,H.H., Kurdzo,E.L.,
            Eyster,C.A., Gladstone,M.N., Gomez-H,L., Llano,E., Meyer,R.,
            Pendas,A.M., Pezza,R.J. and Dawson,D.S.
  TITLE     Shugoshin protects centromere pairing and promotes segregation of
            nonexchange partner chromosomes in meiosis
  JOURNAL   Proc Natl Acad Sci U S A 116 (19), 9417-9422 (2019)
   PUBMED   31019073
REFERENCE   7  (bases 1 to 4989)
  AUTHORS   Duroc,Y., Kumar,R., Ranjha,L., Adam,C., Guerois,R., Md Muntaz,K.,
            Marsolier-Kergoat,M.C., Dingli,F., Laureau,R., Loew,D.,
            Llorente,B., Charbonnier,J.B., Cejka,P. and Borde,V.
  TITLE     Concerted action of the MutLbeta heterodimer and Mer3 helicase
            regulates the global extent of meiotic gene conversion
  JOURNAL   Elife 6, e21900 (2017)
   PUBMED   28051769
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 4989)
  AUTHORS   Ni,L., Xie,H. and Tan,L.
  TITLE     Multiple roles of FOXJ3 in spermatogenesis: A lesson from Foxj3
            conditional knockout mouse models
  JOURNAL   Mol Reprod Dev 83 (12), 1060-1069 (2016)
   PUBMED   27739607
REFERENCE   9  (bases 1 to 4989)
  AUTHORS   Lee,C.Y., Horn,H.F., Stewart,C.L., Burke,B., Bolcun-Filas,E.,
            Schimenti,J.C., Dresser,M.E. and Pezza,R.J.
  TITLE     Mechanism and regulation of rapid telomere prophase movements in
            mouse meiotic chromosomes
  JOURNAL   Cell Rep 11 (4), 551-563 (2015)
   PUBMED   25892231
REFERENCE   10 (bases 1 to 4989)
  AUTHORS   Guiraldelli,M.F., Eyster,C., Wilkerson,J.L., Dresser,M.E. and
            Pezza,R.J.
  TITLE     Mouse HFM1/Mer3 is required for crossover formation and complete
            synapsis of homologous chromosomes during meiosis
  JOURNAL   PLoS Genet 9 (3), e1003383 (2013)
   PUBMED   23555294
  REMARK    GeneRIF: We propose that initial steps of recombination are
            sufficient to support homology recognition, pairing, and initial
            chromosome synapsis and that HFM1 is required to form normal
            numbers of COs and to complete synapsis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC137947.12.
            
            On Oct 4, 2022 this sequence version replaced NM_177873.3.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN01164139 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                AC137947.12        208697-208788       c
            93-194              AC137947.12        207759-207860       c
            195-292             AC137947.12        205741-205838       c
            293-405             AC137947.12        201305-201417       c
            406-709             AC137947.12        200252-200555       c
            710-972             AC137947.12        194332-194594       c
            973-1020            AC137947.12        194000-194047       c
            1021-1091           AC137947.12        187674-187744       c
            1092-1224           AC137947.12        187154-187286       c
            1225-1376           AC137947.12        185850-186001       c
            1377-1454           AC137947.12        184997-185074       c
            1455-1610           AC137947.12        184633-184788       c
            1611-1811           AC137947.12        181357-181557       c
            1812-1900           AC137947.12        181186-181274       c
            1901-1944           AC137947.12        179159-179202       c
            1945-2046           AC137947.12        178872-178973       c
            2047-2192           AC137947.12        178124-178269       c
            2193-2287           AC137947.12        176378-176472       c
            2288-2421           AC137947.12        175626-175759       c
            2422-2469           AC137947.12        172830-172877       c
            2470-2550           AC137947.12        169439-169519       c
            2551-2642           AC137947.12        164721-164812       c
            2643-2749           AC137947.12        163363-163469       c
            2750-2797           AC137947.12        163227-163274       c
            2798-2895           AC137947.12        161731-161828       c
            2896-3027           AC137947.12        161519-161650       c
            3028-3113           AC137947.12        157771-157856       c
            3114-3197           AC137947.12        157131-157214       c
            3198-3355           AC137947.12        156862-157019       c
            3356-3459           AC137947.12        155249-155352       c
            3460-3606           AC137947.12        154709-154855       c
            3607-3687           AC137947.12        140466-140546       c
            3688-3803           AC137947.12        139805-139920       c
            3804-3883           AC137947.12        137669-137748       c
            3884-3987           AC137947.12        136046-136149       c
            3988-4101           AC137947.12        130592-130705       c
            4102-4192           AC137947.12        128313-128403       c
            4193-4304           AC137947.12        125388-125499       c
            4305-4456           AC137947.12        124542-124693       c
            4457-4989           AC137947.12        123124-123656       c
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 51.43 cM"
     gene            1..4989
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="HFM1, ATP-dependent DNA helicase homolog"
                     /db_xref="GeneID:330149"
                     /db_xref="MGI:MGI:3036246"
     exon            1..92
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            93..194
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            195..292
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     CDS             222..4526
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; SEC63
                     domain containing 1; probable ATP-dependent DNA helicase
                     HFM1"
                     /codon_start=1
                     /product="probable ATP-dependent DNA helicase HFM1 isoform
                     1"
                     /protein_id="NP_808541.2"
                     /db_xref="CCDS:CCDS51582.1"
                     /db_xref="GeneID:330149"
                     /db_xref="MGI:MGI:3036246"
                     /translation="MPKSDDCFFSMDNLFFSSPDETENFPVKEKSLDWFLPPAPLISE
                     IPDIQELEEEIESYKLLGKGKMPRMLTSNLKIINEDTNCISPTQKIHFSYNVHEQDYL
                     NLGGSNNNDMSHVAGKLMYGSSQKYKNHMGAKSPSARSSPGDTKLHDVAEDRQGTSAF
                     KKRLSRTCDSEHDCDYADGSLNLSSHISPVKLTQTKISKENAWTCSNSKQKRQYSTNK
                     FKANDAFSASGIGKDIFKAPSFPAASQPHDIQGITPNGLGSLKAVTEIPAKFRNIFKE
                     FPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEVPLPWLNMKIVY
                     MAPIKALCSQRFDDWKEKFGPVGLNCKELTGDTVMDDLFEIQHANIIITTPEKWDSVT
                     RKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSRDLESASPVPVR
                     FVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPCSSSQTEFKFD
                     LALNYKVYSVIRTYSDQKPTLVFCSTRKGVQQAASVLVKDAKFIISVEQKLRLQKSAY
                     SIRDSKLKDTLVYGVGYHHAGMELSDRKLVEGLFTSGDLPVLFTTSTLAMGMNMPAHL
                     VVIKSTMHYSGGVFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLAC
                     NDTVESSLHRHLIEHLNAEIVLHTITDVNIALDWIRSTMLYIRALKNPSHYGFSSGLN
                     KDGIEAKLQELCLKNLKDLSSLDLIKMDEDVNFKPTEAGRLMAWYYITFETVKKFCAI
                     SGKETLLDLISMISSCNEFLDVQLRISEKRILNTLNKDPNRITIRFPMAERIKTREMK
                     VNCLIQAQLGCIPIQDFALTQDTVKIFRNGSRIARWLSDFVAAQEKKFAVLLNSVILT
                     KCFKCKLWENSKHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPF
                     GTQIKEAVAHLPKYELEVEQIARYSDIKAEILVTIILRNFEQLQTKRTAPDFHYATLI
                     IGDADNQVVFKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKF
                     TVFYFGPRKFVNETAMERSSETDISHSDYSGRATATGSSKGMATCKKPGNRECHHHCK
                     NKHACGHDCCKIGVAQKPEVKESAMSSYLSDLKSRDAVSSLPLAKRLKIQMNKSQNVD
                     LKEFGFTPRPSLSSISRSEYLNTPELSILEQRNQHEIYGKVQQGPSEYRDKEVLGVNL
                     ELGNEVWDDFDDESLIEVMSLSADAEKMAASGFGDTRDSSLGGSKLPFQKSSSRFQRD
                     NSNSFASSPGKPDAYLRDCSRSSFGLSSVAEIPQRAENASLANLQERRPLTLSPVIER
                     MCFAHSKKTPQSPKFKEVDIFIGNSGSKKEIDLSKYYPDDAAEEMKALLGIFNGIF"
     exon            293..405
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            406..709
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            710..972
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1020
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1091
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1092..1224
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1376
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1454
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1610
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1811
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1900
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..1944
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2046
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2047..2192
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2287
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2288..2421
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..2469
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2470..2550
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2642
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2643..2749
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2797
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2798..2895
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2896..3027
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3028..3113
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3114..3197
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3198..3355
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3356..3459
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3606
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3607..3687
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3688..3803
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3804..3883
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3884..3987
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3988..4101
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4102..4192
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4193..4304
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4305..4456
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4457..4989
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4570..4575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="hexamer: ATTAAA"
     polyA_site      4599
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="major polyA site"
     regulatory      4752..4757
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="hexamer: AATAAT"
     polyA_site      4772
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
     regulatory      4969..4974
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="hexamer: AATAAA"
     polyA_site      4989
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="major polyA site"
ORIGIN      
        1 ctgttctcta gtccgctgtc ctcccctcgc gctcagttgt ggtgcgtggt cgctgggcct
       61 gggtctctgc accggcggat gatttctgac gggaactgtt ccaggttatt gaagcacatt
      121 aagaagaaga aacaaacatc ccttttttgc tttctaggga gaaagataaa ctaaacacac
      181 agtaacaatt acagatttca gacaaggtct gtttttcaac aatgccaaag tcagacgatt
      241 gcttcttttc catggataat ttgtttttca gtagcccaga tgaaactgaa aactttccag
      301 ttaaggaaaa atcactggat tggtttctcc ctcctgctcc actgatttca gaaattccag
      361 atattcaaga gttagaggaa gaaattgaaa gttataagct actagggaaa ggaaagatgc
      421 caagaatgtt aacatcaaac ttgaagatca ttaatgaaga cacaaattgt atttcaccaa
      481 cacaaaaaat ccatttttcc tataatgtac atgaacagga ttacctaaat ttaggaggat
      541 caaataacaa tgacatgtcc catgtagctg ggaagctgat gtatggttct tctcagaaat
      601 ataaaaatca catgggtgct aagagtccat ctgcaaggag tagtcctggt gatacaaaat
      661 tacatgatgt ggcagaagac agacagggca catcagcatt caaaaaaaga ctgtctagaa
      721 catgtgacag tgaacatgac tgtgactatg ctgatggaag tttaaacttg agttctcata
      781 tcagcccagt gaaacttacc caaacgaaaa taagcaaaga gaatgcatgg acgtgtagca
      841 atagtaagca gaaacgtcag tattctacaa acaaattcaa agcaaatgat gctttttctg
      901 cttctggaat tggaaaagac atattcaaag caccatcatt tcctgctgca tctcagcctc
      961 atgatattca aggaataacc ccaaatggct taggttcttt gaaagctgtc acggaaatcc
     1021 cggcaaaatt tagaaatata ttcaaagaat tcccttactt caactatata caatccaaag
     1081 cctttgatga tcttctttac acagatagga attttgtgat ttgtgccccc actggttctg
     1141 ggaaaactgt agtgtttgag ctagcgataa caagattgtt aatggaagta ccattgccat
     1201 ggctaaacat gaagattgtt tacatggcac caataaaagc cctttgtagt cagcggtttg
     1261 atgactggaa agaaaagttt ggaccagtag gcttgaattg taaagaactt actggtgata
     1321 cagtaatgga tgacctgttt gagattcagc atgcaaatat aattataaca actccagaga
     1381 agtgggacag tgtgactagg aagtggagag acaactcttt tatccaactg gttcgactgt
     1441 ttctcattga tgaggtgcat gttattaaag atgaaaatcg tggtccaact cttgaagttg
     1501 tagttagcag aatgaaaact gtacagtctt tatctaggga tttagaaagt gccagccctg
     1561 ttccagtgag atttgtagct gtgtctgcaa caattcccaa tgctgaggat atcgcagaat
     1621 ggctttcaga tggtgagaga cctgctgtat gtctcaaaat ggatgagagc catagacctg
     1681 tgaaacttca gaaagtagtc ctgggttttc cctgtagtag tagccaaact gaatttaagt
     1741 ttgatttggc cctcaactat aaagtgtaca gtgttattcg aacatactcc gatcagaagc
     1801 ccacacttgt gttttgttca acaaggaaag gtgtgcagca ggctgcttct gttcttgtga
     1861 aggatgctaa gtttatcatc tctgtggagc agaaactacg gttgcagaag tctgcatatt
     1921 ctataaggga ttcaaagctg aaagatacct tggtatatgg tgttggttac catcatgctg
     1981 gaatggagct gtcagataga aaattggttg aaggattgtt tacctctgga gatctaccag
     2041 ttcttttcac taccagtact ctagctatgg gcatgaacat gccggctcac ctggttgtta
     2101 taaagtctac catgcactac agtggaggtg tgtttgaaga gtacagcgag accgacatcc
     2161 tgcagatgat tgggagagct ggcagacccc agtttgacac tacagctact gcagtcatta
     2221 tgactcgact aagtacaaga gagaagtacg tgcagatgct agcttgtaac gacactgtag
     2281 agagcagttt gcacagacat cttattgaac atttaaatgc agaaatagtg ctgcatacca
     2341 tcacagacgt gaacattgct ctggactgga tccgatcaac aatgctgtat atcagagctc
     2401 tgaaaaaccc atctcactat ggtttttcat ctggattgaa caaagatggg attgaagcaa
     2461 aattacaaga attatgtttg aagaatctga aagatttatc atcactagac ctgataaaga
     2521 tggatgaaga tgttaatttt aaaccaacag aggcaggaag actaatggct tggtattata
     2581 ttacatttga gacagtcaag aaattttgtg caatcagtgg aaaagaaact ttattggatt
     2641 taatttcaat gatatcgagc tgcaatgagt ttctagatgt gcagctgagg ataagtgaaa
     2701 agagaatact gaacacctta aacaaagacc caaaccggat aactatcaga tttccaatgg
     2761 cagaaagaat taaaacaaga gaaatgaaag taaactgtct tattcaggct cagctaggat
     2821 gcattcctat acaagatttt gctttgacac aagatacagt gaagattttc agaaatggct
     2881 cacgaattgc aagatggttg tcagattttg tggctgctca ggaaaagaag tttgctgtgc
     2941 ttttaaacag tgtgatttta actaaatgtt ttaagtgtaa actctgggaa aactctaaac
     3001 atgtatcaaa acagctggat aaaattggca tatcattgtc aaataccatg gtaaatgcag
     3061 gcttaacttc ttttaaaaaa atagaagaag caaatgcaag agaacttgaa ctgattttaa
     3121 atagacaccc accatttggg acccagataa aagaagctgt ggcgcatctg ccgaagtatg
     3181 agcttgaggt ggagcagatt gcaagataca gtgatatcaa ggcagaaata ctggtgacca
     3241 ttatattaag aaactttgag cagctgcaaa ctaaaagaac agcaccagac ttccactatg
     3301 ccaccttaat cataggtgat gcagataacc aagtcgtttt taagcacaaa attatggatt
     3361 ctgtcttgtt aaaaagtgga aattgggtta aaaaaattga tgtgaagaga gctcttatat
     3421 cagaagatct cagcataaat ctaattagtt ctgattatgt tggtcttgat attcaccaga
     3481 aatttacagt cttttatttc ggacccagga agtttgtaaa tgaaacagct atggaaagaa
     3541 gctcagaaac agatatttct cattctgact actcaggtag agccactgca acaggatcca
     3601 gtaaaggaat ggctacctgc aaaaaacctg ggaacagaga atgccatcat cactgtaaaa
     3661 ataaacacgc atgtggacat gactgctgta aaattggagt tgcacaaaag ccggaagtta
     3721 aagagtcagc aatgtcttca tatctgtctg acttaaaaag cagagatgct gtttcttctc
     3781 ttcctctagc caagcgactc aagatacaga tgaacaaatc tcaaaatgtg gaccttaaag
     3841 agtttggttt tactccaagg ccttcccttt ccagtatatc aaggtcagaa tatttaaata
     3901 cccctgaatt gtcaatatta gaacagagga atcagcatga aatctatgga aaagttcaac
     3961 agggaccatc tgaatatcgt gacaaagaag ttctgggtgt gaacttagag ctgggaaatg
     4021 aagtctggga tgactttgat gatgagagct taatagaagt tatgagcctt tcagctgatg
     4081 ctgagaagat ggctgcatca ggatttggag acactcgtga ttcaagtctc ggcggaagta
     4141 agctaccctt ccaaaagtca agcagcagat tccaaagaga caactcaaac agttttgctt
     4201 cgtcgcctgg gaagccagat gcttatttgc gagattgttc caggtccagc ttcgggctgt
     4261 cctctgtggc agagatccct cagcgggctg agaatgcaag tcttgccaat ttacaagaga
     4321 ggagaccgct gactctctca ccagtgattg agaggatgtg ctttgcccac tctaagaaaa
     4381 caccacagtc tcccaagttt aaggaagtgg atatttttat tggaaacagt ggctctaaaa
     4441 aggaaattga tcttagtaag tattatcctg atgatgcagc tgaagaaatg aaggctcttc
     4501 tgggaatatt taatggcatt ttctaaagaa aaatgtagtt aataaggcaa agtataaaca
     4561 tgtgtaatga ttaaaaatgc tttttatttc taaagataat tgagaattgt tcagagtttt
     4621 tttcttaagt ttgattcatt ccttgacttt cttataacaa aatatatgga ctgataatat
     4681 ttttattttt attgtaactt ctggaagaat gtgtgttttt tcagtaaact gataattaaa
     4741 ttatcttcat aaataatttc ttattaaacc aattttaata ggctctttat ttcattttta
     4801 tattttctta agaaagatgt ctgagaaagc acaataatgc tgtcagtgac catgtgtagt
     4861 cacggtgtat tcgccagcag ggttcctgtg tgtcctgctc tgttgtttgc tcttctgttt
     4921 ttttaagaat ccagtagcct ttgaatctgt tctttcattt tgagcagtaa taaatgaaaa
     4981 tgctaatca
//
