LOCUS       NM_001412561            5089 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus HFM1, ATP-dependent DNA helicase homolog (Hfm1),
            transcript variant 4, mRNA.
ACCESSION   NM_001412561 XM_011249484
VERSION     NM_001412561.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ito,M., Yun,Y., Kulkarni,D.S., Lee,S., Sandhu,S., Nunez,B., Hu,L.,
            Lee,K., Lim,N., Hirota,R.M., Prendergast,R., Huang,C., Huang,I. and
            Hunter,N.
  TITLE     Distinct and interdependent functions of three RING proteins
            regulate recombination during mammalian meiosis
  JOURNAL   Proc Natl Acad Sci U S A 122 (2), e2412961121 (2025)
   PUBMED   39761402
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   He,Y., Wang,H., Hong,T., Hu,L., Gao,C., Gao,L., Cui,Y., Tan,R.,
            Pu,D. and Wu,J.
  TITLE     HFM1 is essential for the germ cell intercellular bridge transport
            in primordial follicle formation in mice
  JOURNAL   Cell Mol Life Sci 82 (1), 28 (2024)
   PUBMED   39725823
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5089)
  AUTHORS   Rezaei,M., Liang,M., Yalcin,Z., Martin,J.H., Kazemi,P., Bareke,E.,
            Ge,Z.J., Fardaei,M., Benadiva,C., Hemida,R., Hassan,A., Maher,G.J.,
            Abdalla,E., Buckett,W., Bolze,P.A., Sandhu,I., Duman,O.,
            Agrawal,S., Qian,J., Vallian Broojeni,J., Bhati,L., Miron,P.,
            Allias,F., Selim,A., Fisher,R.A., Seckl,M.J., Sauthier,P.,
            Touitou,I., Tan,S.L., Majewski,J., Taketo,T. and Slim,R.
  TITLE     Defects in meiosis I contribute to the genesis of androgenetic
            hydatidiform moles
  JOURNAL   J Clin Invest 134 (22), e170669 (2024)
   PUBMED   39545410
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5089)
  AUTHORS   Zhong,C., Wang,H., Yuan,X., He,Y., Cong,J., Yang,R., Ma,W., Gao,L.,
            Gao,C., Cui,Y., Wu,J., Tan,R. and Pu,D.
  TITLE     The crucial role of HFM1 in regulating FUS ubiquitination and
            localization for oocyte meiosis prophase I progression in mice
  JOURNAL   Biol Res 57 (1), 36 (2024)
   PUBMED   38822414
  REMARK    GeneRIF: The crucial role of HFM1 in regulating FUS ubiquitination
            and localization for oocyte meiosis prophase I progression in mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5089)
  AUTHORS   Xie,X., Murtaza,G., Li,Y., Zhou,J., Ye,J., Khan,R., Jiang,L.,
            Khan,I., Zubair,M., Yin,H., Jiang,H., Liu,W., Shi,B., Hou,X.,
            Gong,C., Fan,S., Wang,Y., Jiang,X., Zhang,Y., Zhang,H., Ma,H. and
            Shi,Q.
  TITLE     Biallelic HFM1 variants cause non-obstructive azoospermia with
            meiotic arrest in humans by impairing crossover formation to
            varying degrees
  JOURNAL   Hum Reprod 37 (7), 1664-1677 (2022)
   PUBMED   35526155
  REMARK    GeneRIF: Biallelic HFM1 variants cause non-obstructive azoospermia
            with meiotic arrest in humans by impairing crossover formation to
            varying degrees.
REFERENCE   6  (bases 1 to 5089)
  AUTHORS   Previato de Almeida,L., Evatt,J.M., Chuong,H.H., Kurdzo,E.L.,
            Eyster,C.A., Gladstone,M.N., Gomez-H,L., Llano,E., Meyer,R.,
            Pendas,A.M., Pezza,R.J. and Dawson,D.S.
  TITLE     Shugoshin protects centromere pairing and promotes segregation of
            nonexchange partner chromosomes in meiosis
  JOURNAL   Proc Natl Acad Sci U S A 116 (19), 9417-9422 (2019)
   PUBMED   31019073
REFERENCE   7  (bases 1 to 5089)
  AUTHORS   Duroc,Y., Kumar,R., Ranjha,L., Adam,C., Guerois,R., Md Muntaz,K.,
            Marsolier-Kergoat,M.C., Dingli,F., Laureau,R., Loew,D.,
            Llorente,B., Charbonnier,J.B., Cejka,P. and Borde,V.
  TITLE     Concerted action of the MutLbeta heterodimer and Mer3 helicase
            regulates the global extent of meiotic gene conversion
  JOURNAL   Elife 6, e21900 (2017)
   PUBMED   28051769
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 5089)
  AUTHORS   Ni,L., Xie,H. and Tan,L.
  TITLE     Multiple roles of FOXJ3 in spermatogenesis: A lesson from Foxj3
            conditional knockout mouse models
  JOURNAL   Mol Reprod Dev 83 (12), 1060-1069 (2016)
   PUBMED   27739607
REFERENCE   9  (bases 1 to 5089)
  AUTHORS   Lee,C.Y., Horn,H.F., Stewart,C.L., Burke,B., Bolcun-Filas,E.,
            Schimenti,J.C., Dresser,M.E. and Pezza,R.J.
  TITLE     Mechanism and regulation of rapid telomere prophase movements in
            mouse meiotic chromosomes
  JOURNAL   Cell Rep 11 (4), 551-563 (2015)
   PUBMED   25892231
REFERENCE   10 (bases 1 to 5089)
  AUTHORS   Guiraldelli,M.F., Eyster,C., Wilkerson,J.L., Dresser,M.E. and
            Pezza,R.J.
  TITLE     Mouse HFM1/Mer3 is required for crossover formation and complete
            synapsis of homologous chromosomes during meiosis
  JOURNAL   PLoS Genet 9 (3), e1003383 (2013)
   PUBMED   23555294
  REMARK    GeneRIF: We propose that initial steps of recombination are
            sufficient to support homology recognition, pairing, and initial
            chromosome synapsis and that HFM1 is required to form normal
            numbers of COs and to complete synapsis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC137947.12.
            
            On Oct 6, 2022 this sequence version replaced XM_011249484.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849381, SAMN00849382
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression,
                                         longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-66                AC137947.12        209125-209190       c
            67-168              AC137947.12        207759-207860       c
            169-266             AC137947.12        205741-205838       c
            267-379             AC137947.12        201305-201417       c
            380-683             AC137947.12        200252-200555       c
            684-946             AC137947.12        194332-194594       c
            947-994             AC137947.12        194000-194047       c
            995-1065            AC137947.12        187674-187744       c
            1066-1198           AC137947.12        187154-187286       c
            1199-1350           AC137947.12        185850-186001       c
            1351-1428           AC137947.12        184997-185074       c
            1429-1584           AC137947.12        184633-184788       c
            1585-1785           AC137947.12        181357-181557       c
            1786-1874           AC137947.12        181186-181274       c
            1875-1918           AC137947.12        179159-179202       c
            1919-2020           AC137947.12        178872-178973       c
            2021-2166           AC137947.12        178124-178269       c
            2167-2261           AC137947.12        176378-176472       c
            2262-2395           AC137947.12        175626-175759       c
            2396-2443           AC137947.12        172830-172877       c
            2444-2524           AC137947.12        169439-169519       c
            2525-2616           AC137947.12        164721-164812       c
            2617-2723           AC137947.12        163363-163469       c
            2724-2771           AC137947.12        163227-163274       c
            2772-2869           AC137947.12        161731-161828       c
            2870-3001           AC137947.12        161519-161650       c
            3002-3087           AC137947.12        157771-157856       c
            3088-3171           AC137947.12        157131-157214       c
            3172-3329           AC137947.12        156862-157019       c
            3330-3433           AC137947.12        155249-155352       c
            3434-3580           AC137947.12        154709-154855       c
            3581-3661           AC137947.12        140466-140546       c
            3662-3777           AC137947.12        139805-139920       c
            3778-3857           AC137947.12        137669-137748       c
            3858-3961           AC137947.12        136046-136149       c
            3962-4201           AC137947.12        130592-130831       c
            4202-4292           AC137947.12        128313-128403       c
            4293-4404           AC137947.12        125388-125499       c
            4405-4556           AC137947.12        124542-124693       c
            4557-5089           AC137947.12        123124-123656       c
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 51.43 cM"
     gene            1..5089
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="HFM1, ATP-dependent DNA helicase homolog"
                     /db_xref="GeneID:330149"
                     /db_xref="MGI:MGI:3036246"
     exon            1..66
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            67..168
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            169..266
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     CDS             196..4626
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /EC_number="3.6.4.12"
                     /note="isoform 4 is encoded by transcript variant 4; SEC63
                     domain containing 1; probable ATP-dependent DNA helicase
                     HFM1"
                     /codon_start=1
                     /product="probable ATP-dependent DNA helicase HFM1 isoform
                     4"
                     /protein_id="NP_001399490.1"
                     /db_xref="GeneID:330149"
                     /db_xref="MGI:MGI:3036246"
                     /translation="MPKSDDCFFSMDNLFFSSPDETENFPVKEKSLDWFLPPAPLISE
                     IPDIQELEEEIESYKLLGKGKMPRMLTSNLKIINEDTNCISPTQKIHFSYNVHEQDYL
                     NLGGSNNNDMSHVAGKLMYGSSQKYKNHMGAKSPSARSSPGDTKLHDVAEDRQGTSAF
                     KKRLSRTCDSEHDCDYADGSLNLSSHISPVKLTQTKISKENAWTCSNSKQKRQYSTNK
                     FKANDAFSASGIGKDIFKAPSFPAASQPHDIQGITPNGLGSLKAVTEIPAKFRNIFKE
                     FPYFNYIQSKAFDDLLYTDRNFVICAPTGSGKTVVFELAITRLLMEVPLPWLNMKIVY
                     MAPIKALCSQRFDDWKEKFGPVGLNCKELTGDTVMDDLFEIQHANIIITTPEKWDSVT
                     RKWRDNSFIQLVRLFLIDEVHVIKDENRGPTLEVVVSRMKTVQSLSRDLESASPVPVR
                     FVAVSATIPNAEDIAEWLSDGERPAVCLKMDESHRPVKLQKVVLGFPCSSSQTEFKFD
                     LALNYKVYSVIRTYSDQKPTLVFCSTRKGVQQAASVLVKDAKFIISVEQKLRLQKSAY
                     SIRDSKLKDTLVYGVGYHHAGMELSDRKLVEGLFTSGDLPVLFTTSTLAMGMNMPAHL
                     VVIKSTMHYSGGVFEEYSETDILQMIGRAGRPQFDTTATAVIMTRLSTREKYVQMLAC
                     NDTVESSLHRHLIEHLNAEIVLHTITDVNIALDWIRSTMLYIRALKNPSHYGFSSGLN
                     KDGIEAKLQELCLKNLKDLSSLDLIKMDEDVNFKPTEAGRLMAWYYITFETVKKFCAI
                     SGKETLLDLISMISSCNEFLDVQLRISEKRILNTLNKDPNRITIRFPMAERIKTREMK
                     VNCLIQAQLGCIPIQDFALTQDTVKIFRNGSRIARWLSDFVAAQEKKFAVLLNSVILT
                     KCFKCKLWENSKHVSKQLDKIGISLSNTMVNAGLTSFKKIEEANARELELILNRHPPF
                     GTQIKEAVAHLPKYELEVEQIARYSDIKAEILVTIILRNFEQLQTKRTAPDFHYATLI
                     IGDADNQVVFKHKIMDSVLLKSGNWVKKIDVKRALISEDLSINLISSDYVGLDIHQKF
                     TVFYFGPRKFVNETAMERSSETDISHSDYSGRATATGSSKGMATCKKPGNRECHHHCK
                     NKHACGHDCCKIGVAQKPEVKESAMSSYLSDLKSRDAVSSLPLAKRLKIQMNKSQNVD
                     LKEFGFTPRPSLSSISRSEYLNTPELSILEQRNQHEIYGKVQQGPSEYRDKDFPRWDY
                     CTTSSEKTPQTVMFSRHANIPGNHRVADEDKSKSSEVLGVNLELGNEVWDDFDDESLI
                     EVMSLSADAEKMAASGFGDTRDSSLGGSKLPFQKSSSRFQRDNSNSFASSPGKPDAYL
                     RDCSRSSFGLSSVAEIPQRAENASLANLQERRPLTLSPVIERMCFAHSKKTPQSPKFK
                     EVDIFIGNSGSKKEIDLSKYYPDDAAEEMKALLGIFNGIF"
     exon            267..379
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            380..683
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            684..946
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            947..994
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            995..1065
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1198
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1199..1350
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1428
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1429..1584
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1785
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1786..1874
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1918
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2020
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2166
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2261
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2262..2395
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2443
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2444..2524
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2616
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2723
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2724..2771
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2772..2869
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..3001
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3087
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3171
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3172..3329
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3330..3433
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3434..3580
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3581..3661
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3662..3777
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3778..3857
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3858..3961
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            3962..4201
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4202..4292
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4293..4404
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4405..4556
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     exon            4557..5089
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4670..4675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="hexamer: ATTAAA"
     polyA_site      4699
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="major polyA site"
     regulatory      4852..4857
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="hexamer: AATAAT"
     polyA_site      4872
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
     regulatory      5069..5074
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="hexamer: AATAAA"
     polyA_site      5089
                     /gene="Hfm1"
                     /gene_synonym="A330009G12Rik; Gm1046; Mer3; Sec63d1"
                     /note="major polyA site"
ORIGIN      
        1 ccccccggca ggtggctgtg gcctgggcgg gacgcgcgcg cctcgcgtgg gcgcgtgggg
       61 ggctcggaac tgttccaggt tattgaagca cattaagaag aagaaacaaa catccctttt
      121 ttgctttcta gggagaaaga taaactaaac acacagtaac aattacagat ttcagacaag
      181 gtctgttttt caacaatgcc aaagtcagac gattgcttct tttccatgga taatttgttt
      241 ttcagtagcc cagatgaaac tgaaaacttt ccagttaagg aaaaatcact ggattggttt
      301 ctccctcctg ctccactgat ttcagaaatt ccagatattc aagagttaga ggaagaaatt
      361 gaaagttata agctactagg gaaaggaaag atgccaagaa tgttaacatc aaacttgaag
      421 atcattaatg aagacacaaa ttgtatttca ccaacacaaa aaatccattt ttcctataat
      481 gtacatgaac aggattacct aaatttagga ggatcaaata acaatgacat gtcccatgta
      541 gctgggaagc tgatgtatgg ttcttctcag aaatataaaa atcacatggg tgctaagagt
      601 ccatctgcaa ggagtagtcc tggtgataca aaattacatg atgtggcaga agacagacag
      661 ggcacatcag cattcaaaaa aagactgtct agaacatgtg acagtgaaca tgactgtgac
      721 tatgctgatg gaagtttaaa cttgagttct catatcagcc cagtgaaact tacccaaacg
      781 aaaataagca aagagaatgc atggacgtgt agcaatagta agcagaaacg tcagtattct
      841 acaaacaaat tcaaagcaaa tgatgctttt tctgcttctg gaattggaaa agacatattc
      901 aaagcaccat catttcctgc tgcatctcag cctcatgata ttcaaggaat aaccccaaat
      961 ggcttaggtt ctttgaaagc tgtcacggaa atcccggcaa aatttagaaa tatattcaaa
     1021 gaattccctt acttcaacta tatacaatcc aaagcctttg atgatcttct ttacacagat
     1081 aggaattttg tgatttgtgc ccccactggt tctgggaaaa ctgtagtgtt tgagctagcg
     1141 ataacaagat tgttaatgga agtaccattg ccatggctaa acatgaagat tgtttacatg
     1201 gcaccaataa aagccctttg tagtcagcgg tttgatgact ggaaagaaaa gtttggacca
     1261 gtaggcttga attgtaaaga acttactggt gatacagtaa tggatgacct gtttgagatt
     1321 cagcatgcaa atataattat aacaactcca gagaagtggg acagtgtgac taggaagtgg
     1381 agagacaact cttttatcca actggttcga ctgtttctca ttgatgaggt gcatgttatt
     1441 aaagatgaaa atcgtggtcc aactcttgaa gttgtagtta gcagaatgaa aactgtacag
     1501 tctttatcta gggatttaga aagtgccagc cctgttccag tgagatttgt agctgtgtct
     1561 gcaacaattc ccaatgctga ggatatcgca gaatggcttt cagatggtga gagacctgct
     1621 gtatgtctca aaatggatga gagccataga cctgtgaaac ttcagaaagt agtcctgggt
     1681 tttccctgta gtagtagcca aactgaattt aagtttgatt tggccctcaa ctataaagtg
     1741 tacagtgtta ttcgaacata ctccgatcag aagcccacac ttgtgttttg ttcaacaagg
     1801 aaaggtgtgc agcaggctgc ttctgttctt gtgaaggatg ctaagtttat catctctgtg
     1861 gagcagaaac tacggttgca gaagtctgca tattctataa gggattcaaa gctgaaagat
     1921 accttggtat atggtgttgg ttaccatcat gctggaatgg agctgtcaga tagaaaattg
     1981 gttgaaggat tgtttacctc tggagatcta ccagttcttt tcactaccag tactctagct
     2041 atgggcatga acatgccggc tcacctggtt gttataaagt ctaccatgca ctacagtgga
     2101 ggtgtgtttg aagagtacag cgagaccgac atcctgcaga tgattgggag agctggcaga
     2161 ccccagtttg acactacagc tactgcagtc attatgactc gactaagtac aagagagaag
     2221 tacgtgcaga tgctagcttg taacgacact gtagagagca gtttgcacag acatcttatt
     2281 gaacatttaa atgcagaaat agtgctgcat accatcacag acgtgaacat tgctctggac
     2341 tggatccgat caacaatgct gtatatcaga gctctgaaaa acccatctca ctatggtttt
     2401 tcatctggat tgaacaaaga tgggattgaa gcaaaattac aagaattatg tttgaagaat
     2461 ctgaaagatt tatcatcact agacctgata aagatggatg aagatgttaa ttttaaacca
     2521 acagaggcag gaagactaat ggcttggtat tatattacat ttgagacagt caagaaattt
     2581 tgtgcaatca gtggaaaaga aactttattg gatttaattt caatgatatc gagctgcaat
     2641 gagtttctag atgtgcagct gaggataagt gaaaagagaa tactgaacac cttaaacaaa
     2701 gacccaaacc ggataactat cagatttcca atggcagaaa gaattaaaac aagagaaatg
     2761 aaagtaaact gtcttattca ggctcagcta ggatgcattc ctatacaaga ttttgctttg
     2821 acacaagata cagtgaagat tttcagaaat ggctcacgaa ttgcaagatg gttgtcagat
     2881 tttgtggctg ctcaggaaaa gaagtttgct gtgcttttaa acagtgtgat tttaactaaa
     2941 tgttttaagt gtaaactctg ggaaaactct aaacatgtat caaaacagct ggataaaatt
     3001 ggcatatcat tgtcaaatac catggtaaat gcaggcttaa cttcttttaa aaaaatagaa
     3061 gaagcaaatg caagagaact tgaactgatt ttaaatagac acccaccatt tgggacccag
     3121 ataaaagaag ctgtggcgca tctgccgaag tatgagcttg aggtggagca gattgcaaga
     3181 tacagtgata tcaaggcaga aatactggtg accattatat taagaaactt tgagcagctg
     3241 caaactaaaa gaacagcacc agacttccac tatgccacct taatcatagg tgatgcagat
     3301 aaccaagtcg tttttaagca caaaattatg gattctgtct tgttaaaaag tggaaattgg
     3361 gttaaaaaaa ttgatgtgaa gagagctctt atatcagaag atctcagcat aaatctaatt
     3421 agttctgatt atgttggtct tgatattcac cagaaattta cagtctttta tttcggaccc
     3481 aggaagtttg taaatgaaac agctatggaa agaagctcag aaacagatat ttctcattct
     3541 gactactcag gtagagccac tgcaacagga tccagtaaag gaatggctac ctgcaaaaaa
     3601 cctgggaaca gagaatgcca tcatcactgt aaaaataaac acgcatgtgg acatgactgc
     3661 tgtaaaattg gagttgcaca aaagccggaa gttaaagagt cagcaatgtc ttcatatctg
     3721 tctgacttaa aaagcagaga tgctgtttct tctcttcctc tagccaagcg actcaagata
     3781 cagatgaaca aatctcaaaa tgtggacctt aaagagtttg gttttactcc aaggccttcc
     3841 ctttccagta tatcaaggtc agaatattta aatacccctg aattgtcaat attagaacag
     3901 aggaatcagc atgaaatcta tggaaaagtt caacagggac catctgaata tcgtgacaaa
     3961 gattttccta gatgggacta ttgtacaact tcaagtgaaa agacccctca aacggtgatg
     4021 ttcagcaggc atgctaatat acctggaaac cacagagttg cagatgaaga caaaagcaag
     4081 tcttcagaag ttctgggtgt gaacttagag ctgggaaatg aagtctggga tgactttgat
     4141 gatgagagct taatagaagt tatgagcctt tcagctgatg ctgagaagat ggctgcatca
     4201 ggatttggag acactcgtga ttcaagtctc ggcggaagta agctaccctt ccaaaagtca
     4261 agcagcagat tccaaagaga caactcaaac agttttgctt cgtcgcctgg gaagccagat
     4321 gcttatttgc gagattgttc caggtccagc ttcgggctgt cctctgtggc agagatccct
     4381 cagcgggctg agaatgcaag tcttgccaat ttacaagaga ggagaccgct gactctctca
     4441 ccagtgattg agaggatgtg ctttgcccac tctaagaaaa caccacagtc tcccaagttt
     4501 aaggaagtgg atatttttat tggaaacagt ggctctaaaa aggaaattga tcttagtaag
     4561 tattatcctg atgatgcagc tgaagaaatg aaggctcttc tgggaatatt taatggcatt
     4621 ttctaaagaa aaatgtagtt aataaggcaa agtataaaca tgtgtaatga ttaaaaatgc
     4681 tttttatttc taaagataat tgagaattgt tcagagtttt tttcttaagt ttgattcatt
     4741 ccttgacttt cttataacaa aatatatgga ctgataatat ttttattttt attgtaactt
     4801 ctggaagaat gtgtgttttt tcagtaaact gataattaaa ttatcttcat aaataatttc
     4861 ttattaaacc aattttaata ggctctttat ttcattttta tattttctta agaaagatgt
     4921 ctgagaaagc acaataatgc tgtcagtgac catgtgtagt cacggtgtat tcgccagcag
     4981 ggttcctgtg tgtcctgctc tgttgtttgc tcttctgttt ttttaagaat ccagtagcct
     5041 ttgaatctgt tctttcattt tgagcagtaa taaatgaaaa tgctaatca
//
