LOCUS       NM_025992               5279 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus hect domain and RLD 6 (Herc6), mRNA.
ACCESSION   NM_025992 XM_355768
VERSION     NM_025992.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Qin,Y., Wang,M., Meng,X., Wang,M., Jiang,H., Gao,Y., Li,J.,
            Zhao,C., Han,C., Zhao,W. and Zheng,X.
  TITLE     ISGylation by HERCs facilitates STING activation
  JOURNAL   Cell Rep 43 (5), 114135 (2024)
   PUBMED   38652662
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5279)
  AUTHORS   Qin,Y., Meng,X., Wang,M., Liang,W., Xu,R., Chen,J., Song,H., Fu,Y.,
            Li,J., Gao,C., Jia,M., Zhao,C. and Zhao,W.
  TITLE     Posttranslational ISGylation of NLRP3 by HERC enzymes facilitates
            inflammasome activation in models of inflammation
  JOURNAL   J Clin Invest 133 (20), e161935 (2023)
   PUBMED   37651190
  REMARK    GeneRIF: Posttranslational ISGylation of NLRP3 by HERC enzymes
            facilitates inflammasome activation in models of inflammation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5279)
  AUTHORS   Du,X., Sheng,J., Chen,Y., He,S., Yang,Y., Huang,Y., Fu,Y., Lie,L.,
            Han,Z., Zhu,B., Liu,H., Wen,Q., Zhou,X., Zhou,C., Hu,S. and Ma,L.
  TITLE     The E3 ligase HERC5 promotes antimycobacterial responses in
            macrophages by ISGylating the phosphatase PTEN
  JOURNAL   Sci Signal 16 (788), eabm1756 (2023)
   PUBMED   37279284
  REMARK    GeneRIF: The E3 ligase HERC5 promotes antimycobacterial responses
            in macrophages by ISGylating the phosphatase PTEN.
REFERENCE   5  (bases 1 to 5279)
  AUTHORS   Suzuki,T., Kikuguchi,C., Nishijima,S., Nagashima,T., Takahashi,A.,
            Okada,M. and Yamamoto,T.
  TITLE     Postnatal liver functional maturation requires Cnot
            complex-mediated decay of mRNAs encoding cell cycle and immature
            liver genes
  JOURNAL   Development 146 (4) (2019)
   PUBMED   30733279
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5279)
  AUTHORS   Nagamachi,A., Matsui,H., Asou,H., Ozaki,Y., Aki,D., Kanai,A.,
            Takubo,K., Suda,T., Nakamura,T., Wolff,L., Honda,H. and Inaba,T.
  TITLE     Haploinsufficiency of SAMD9L, an endosome fusion facilitator,
            causes myeloid malignancies in mice mimicking human diseases with
            monosomy 7
  JOURNAL   Cancer Cell 24 (3), 305-317 (2013)
   PUBMED   24029230
REFERENCE   7  (bases 1 to 5279)
  AUTHORS   Archambaud,C., Nahori,M.A., Soubigou,G., Becavin,C., Laval,L.,
            Lechat,P., Smokvina,T., Langella,P., Lecuit,M. and Cossart,P.
  TITLE     Impact of lactobacilli on orally acquired listeriosis
  JOURNAL   Proc Natl Acad Sci U S A 109 (41), 16684-16689 (2012)
   PUBMED   23012479
REFERENCE   8  (bases 1 to 5279)
  AUTHORS   Ketscher,L., Basters,A., Prinz,M. and Knobeloch,K.P.
  TITLE     mHERC6 is the essential ISG15 E3 ligase in the murine system
  JOURNAL   Biochem Biophys Res Commun 417 (1), 135-140 (2012)
   PUBMED   22138649
  REMARK    GeneRIF: mHERC6 is essential for endogenous murine ISGylation and
            thus represents the dominant ISG15 E3 ligase in mice.
REFERENCE   9  (bases 1 to 5279)
  AUTHORS   Oudshoorn,D., van Boheemen,S., Sanchez-Aparicio,M.T., Rajsbaum,R.,
            Garcia-Sastre,A. and Versteeg,G.A.
  TITLE     HERC6 is the main E3 ligase for global ISG15 conjugation in mouse
            cells
  JOURNAL   PLoS One 7 (1), e29870 (2012)
   PUBMED   22272257
  REMARK    GeneRIF: Data show that the HerC6 N-terminal RCC1-like domain (RLD)
            allows ISG15 conjugation.
REFERENCE   10 (bases 1 to 5279)
  AUTHORS   Tang,Y., Zhong,G., Zhu,L., Liu,X., Shan,Y., Feng,H., Bu,Z., Chen,H.
            and Wang,C.
  TITLE     Herc5 attenuates influenza A virus by catalyzing ISGylation of
            viral NS1 protein
  JOURNAL   J Immunol 184 (10), 5777-5790 (2010)
   PUBMED   20385878
  REMARK    GeneRIF: Expression of the Herc5-mediated ubiquitin-like protein
            ISGylation system can distinctly potentiate interferon-beta-induced
            antiviral effects against influenza A virus.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC142212.4 and AC127307.3.
            
            On Jul 9, 2009 this sequence version replaced NM_025992.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147960.1, SRR17253014.3684761.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849383
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-241               AC142212.4         75698-75938         c
            242-401             AC142212.4         73598-73757         c
            402-478             AC142212.4         72304-72380         c
            479-706             AC142212.4         58274-58501         c
            707-801             AC142212.4         56871-56965         c
            802-929             AC142212.4         52730-52857         c
            930-1066            AC142212.4         49752-49888         c
            1067-1140           AC142212.4         49223-49296         c
            1141-1259           AC142212.4         46009-46127         c
            1260-1319           AC142212.4         45096-45155         c
            1320-1413           AC142212.4         38224-38317         c
            1414-1603           AC142212.4         37296-37485         c
            1604-1758           AC142212.4         30880-31034         c
            1759-1863           AC142212.4         10699-10803         c
            1864-1971           AC142212.4         9758-9865           c
            1972-2109           AC142212.4         4761-4898           c
            2110-2253           AC142212.4         2398-2541           c
            2254-2420           AC127307.3         67945-68111
            2421-2484           AC127307.3         69612-69675
            2485-2555           AC127307.3         70027-70097
            2556-2739           AC127307.3         72002-72185
            2740-2842           AC127307.3         72279-72381
            2843-5279           AC127307.3         72636-75072
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 27.82 cM"
     gene            1..5279
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /note="hect domain and RLD 6"
                     /db_xref="GeneID:67138"
                     /db_xref="MGI:MGI:1914388"
     exon            1..241
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     CDS             46..3057
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /note="hect domain and RLD 5"
                     /codon_start=1
                     /product="E3 ISG15--protein ligase Herc6"
                     /protein_id="NP_080268.1"
                     /db_xref="CCDS:CCDS51796.1"
                     /db_xref="GeneID:67138"
                     /db_xref="MGI:MGI:1914388"
                     /translation="MYFSWAAGSRKPRRLKAGTSGIELLQAASGEHHSLLLFSNHRVY
                     SCGDNSWGQLGQRRDQSTERPEPIQALNDLHVDLVSCGKEHSVAVCHKGKVFAWGAGS
                     EGQLGIGEFKEISFMPTKIKALAGIKIIQVSCGHYHSLALSEDGHVFSWGRNSEGQLG
                     LGKNSRSQAIPQKVKSLEGIPLAQVAAGGTHSFALSLTGTSFGWGSNRSGQLALSGNK
                     VKEQIYKPHSIGALKNLSVIYISCGYEHTAVLTEEGQVFTFGGNSSGQLQPSPRSGQR
                     GPQLIEGIGGRVSQIECASYHTIAYVYTTGQVVSLGRGPSHTSNPTHQEAPAENSDIT
                     CLLAAEDLVDIEVKDIFAGAHANFVTTRRVSHTRSTGVSMKILPEIIRINQSLVKKWR
                     AANKRKDREGAKREISLIFSSSACLTASFLKKRDAGENNLIDVDLKLARDVFKKLTTE
                     KWISSLITTCLEEYLLRDLPYSSPHQEALLVFLLLPECSIMQDPKNWKTLAFEFAKAI
                     HKMGPQSLAFLRTCWASLEVSSLNILVQMLKKAIISQIQYGVATEQYITNIKVLLEVI
                     KEVHKANCQLPESAFIINELSGIFNFDAEAGRMFIRHNDLDCTESSDMVVFSDFLFVF
                     DLPSKIKLMKCDSFVKLMSEVMAFPEKMSSPPYLILKVRRSHLVEDTLRQLRQVEDFD
                     LRKQLSVGFINEIRPEAGGVSSEFFHCIFEEMTDPKYEMFIYPEKGSSMWFPVNPKFE
                     KSSYFLFGILCGLSLHNLKVINLPFPLALYKKLLNQKPSLEDLKELSLPLGRNLQEVL
                     NCEAGDIEELHMYFSIYWDQKDVDLIPDGISVPVNETNKRDYVSKYVDYIFNISIKTI
                     YEEFHRGFYKVCNWDIIRQFQPEELMTAIIGNATCDWKQFENNSKYKDGYDKSHPTIL
                     LFWKAFHDLTLDEKKKFLLFLTGCDRLHVKGLQNEGIVFRCSETFSEEDNPRSLTCHR
                     MLDLPKYSSMRRMKEALQVAINNSTGFVSQE"
     exon            242..401
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            402..478
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            479..706
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            707..801
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            802..929
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1066
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1140
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1259
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1319
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1320..1413
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1603
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1758
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1863
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1971
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            1972..2109
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2253
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2254..2420
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2484
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2555
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2739
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2740..2842
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..5279
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3195..3200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /note="hexamer: AATAAA"
     regulatory      3202..3207
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /note="hexamer: TATAAA"
     polyA_site      3220
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
     polyA_site      3227
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /note="major polyA site"
     regulatory      4758..4763
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
                     /note="hexamer: CATAAA"
     polyA_site      4775
                     /gene="Herc6"
                     /gene_synonym="1700121D12Rik; 2510038N07Rik;
                     4930427L17Rik; CEB1; Herc5"
ORIGIN      
        1 gcccatctga cataccagag ccgcgggtgg ctgtgcaccg cagggatgta tttcagctgg
       61 gctgcaggaa gccggaagcc tcggcgcctg aaggcgggaa ccagcggcat tgaactcttg
      121 caggcagcca gcggggaaca ccactcgctc ctactgttca gcaaccacag ggtgtattcc
      181 tgcggggaca acagctgggg acagctgggc cagaggagag accagagtac cgaaaggcca
      241 gaaccaattc aggcactgaa tgatctgcat gttgacctgg tgagctgtgg gaaggaacac
      301 tctgtggctg tctgccacaa aggaaaggtc ttcgcttggg gagccggttc tgaagggcag
      361 ctggggattg gagaatttaa ggaaataagc tttatgccta cgaaaataaa agctctggct
      421 ggcataaaaa taatccaagt ttcctgtgga cactaccact ctttggcctt atcagaagat
      481 ggccacgtgt tttcatgggg aaggaacagt gaaggacaac tgggcctggg gaagaattcc
      541 cgttctcaag ctattccaca gaaggtgaag tctctggagg ggatcccact ggctcaggtt
      601 gctgcaggtg gcacacacag ttttgctctg tctctcacgg ggacatcatt tggctggggg
      661 agcaacagat ctgggcagtt ggctctcagc gggaacaaag ttaaagaaca aatctacaag
      721 ccgcattcaa ttggtgccct gaagaatctg agtgtgattt atattagctg tggttatgag
      781 catactgcag tgcttactga ggagggacaa gtgtttacat ttggaggcaa tagctctgga
      841 caactacagc ccagccccag gagtggacaa agaggcccac aactcataga aggaattggt
      901 ggccgtgttt cacagataga gtgcgcaagt tatcacacca ttgcatatgt ctacaccact
      961 ggtcaggtgg tatctttagg ccgtggacca agtcacacaa gcaacccaac tcatcaggag
     1021 gcccccgcag agaactctga tattacctgc ctgctagctg cagaggacct tgtggatatt
     1081 gaagtcaaag acatttttgc tggagcacac gccaactttg tgacaactcg tcgggtatca
     1141 catactaggt ctacaggtgt ttccatgaaa atcctgccag aaataatccg aatcaaccag
     1201 tctctggtaa agaaatggag agcagcaaat aaaagaaaag atcgtgaagg cgccaaaaga
     1261 gaaatcagct tgatattttc atcttctgct tgtttgacag cgagctttct gaagaaaaga
     1321 gatgctggag agaataatct cattgatgtg gacttaaaac tggcaagaga tgtcttcaag
     1381 aagttaacaa cagagaaatg gatttcttct ttgataacca cgtgtcttga ggagtatctg
     1441 ctcagagatc ttccatacag ttctccacac caagaagctc tacttgtttt tcttttgctc
     1501 ccagaatgtt ccattatgca ggatcctaag aactggaaga ccctggcatt tgaatttgca
     1561 aaagctattc ataaaatggg tccacagtca ttagcattcc tgagaacgtg ttgggcatct
     1621 ttggaagttt cttcactgaa catactggtt cagatgctaa agaaagccat catctctcaa
     1681 atacagtatg gagttgcaac ggagcagtat attactaata ttaaagttct tttagaagtg
     1741 ataaaagaag tgcataaggc taactgtcaa cttccagaaa gtgccttcat cataaatgag
     1801 ctttctggca tctttaactt tgatgcagag gcaggaagaa tgttcatacg gcataacgat
     1861 ctggactgta cagaatcctc agatatggtt gttttcagtg attttctatt tgtctttgat
     1921 ttgccatcca aaattaaact aatgaaatgt gactcatttg taaaactgat gtctgaagtc
     1981 atggcatttc cagaaaagat gagctcccca ccatacctta tactgaaagt cagacgaagt
     2041 cacctggtgg aagacacatt gcgtcaatta agacaagtgg aagactttga ccttaggaag
     2101 caactatcgg ttggatttat taatgaaatt cgtcctgagg ctgggggagt gagttcagag
     2161 ttcttccact gtatatttga agagatgaca gaccccaaat atgaaatgtt catatatcct
     2221 gaaaagggtt ccagcatgtg gtttccagtc aatcccaagt ttgagaagag cagctatttc
     2281 ctatttggga tcttatgtgg actctcccta cataacttaa aggttatcaa ccttcctttc
     2341 ccactggctt tatataaaaa acttttgaac caaaagccat ctttggaaga cttaaaagaa
     2401 cttagtcttc ccttggggag gaatttgcaa gaagttctaa attgtgaggc tggtgatatt
     2461 gaagaacttc acatgtattt ttctatatac tgggatcaaa aagatgttga tttaattcca
     2521 gatgggatct ctgtgcctgt gaatgaaacc aacaagagag actatgtgtc taagtatgtt
     2581 gattacattt ttaacatctc tataaagacg atttatgaag aatttcatag aggattttac
     2641 aaagtgtgta actgggatat aattaggcaa ttccagcccg aagaactaat gacagcaata
     2701 attggaaatg ctacttgtga ctggaaacaa tttgaaaata attcaaagta taaagatgga
     2761 tatgacaaat cacacccaac tattctgctg ttttggaagg ctttccatga cttaactttg
     2821 gatgaaaaga aaaaattcct cttgtttctt acaggatgtg ataggctgca tgtgaaaggc
     2881 ctacagaatg aaggaatagt attccggtgt tctgaaacct tcagtgaaga agataaccca
     2941 agatcattga catgtcacag gatgctagac ctacctaagt attcctcaat gcgaaggatg
     3001 aaggaagcac ttcaggttgc aatcaacaat agcacgggat ttgtctcaca ggaatgatgc
     3061 agaaacattc acctcccagc agttcacagc ctcgggaact aggtgtttca ctttgggatt
     3121 cttctgtctt tccttaatct aagtagggac aagaggttaa aggattttac ttgaattgca
     3181 cagtttttga gataaataaa ttataaatta atactttagt gaaaaaattc tgtaaaacat
     3241 ttgctgcatt cttaactttg atgcttttga atgtttgttt tggctgaaat atttcctgaa
     3301 caacaatgtt catttaataa tatactaaat atttaaaatg ttcaatattg tgagctaaag
     3361 agagagctca atgtttaaga tcactggctg ctcttccaga ggaccagggc tcagttccca
     3421 gcacccacaa tggctactca cctctcgtct ttaactccag tcctaaggga tccaacaccc
     3481 tcctctggcc tccaagacat tccatggata tggtacacag atatacatgc caccaaaaca
     3541 ctcaaacacc taaattaaaa aaacaaataa aataaaatag tgtttattta aagatttatg
     3601 gtacctataa taaactatta gtttgaaaat attaagaaac catgcttgca aagctttcaa
     3661 cgtgtttttg aagcatatag gtgcatattt tgtattaagg aatataatta gagttgtgat
     3721 tttagaaaat tctaaatgat ttattttcaa cttctaagtg ttgacattta ttattcatca
     3781 aaggtactta tacttagtac agtgttccaa aatacattct aaacatagat aatgttattt
     3841 tagtaaaata ctgaaaacaa tcaagaactc atacctactc atggttggca aaatgggaag
     3901 aagttgaaat atgtcttatt atgatgctat agagtaaatt aatgaagcaa tgtaactgct
     3961 atggaaaatg accaatacat cttgtatact agttctactt aatgttagaa cttaatttga
     4021 attacacagc tttgaaataa tttcataaat ataatttgtc ataagaatga catatatgct
     4081 gctgtgacaa tcactcatca taaaagttcc atttctgtaa agatgttgta tctttataga
     4141 cttatcagac tttatatgtg agatgtttac tggactccaa agaaaccaaa taagctaatt
     4201 aaaaatagat catttaaaga aaataaaatg attgtcatca attgctacaa caaaagcaaa
     4261 tgaatcgtta gaagagggaa tacgcttcag ctaattctcg gattccaaca ttgctccagt
     4321 accagacaaa gatgtcacag agagaggaaa ccatagatat cttacgtgaa cagacaccaa
     4381 aatccttccc tacattttaa tatacttaga acatccaata caatatagag tgtggtgcct
     4441 cacaaccaag tgggaattaa cctggagatg cacttaaaaa tcaactacat ctcaacagac
     4501 tgataggaaa gaatgtccat atagtacctc agaatagaca gaaaagtaac tgagcaaaat
     4561 gtagcaccta gtcatgtatg agaaagccaa caaggcctaa aggaaactgc caatttgata
     4621 aaacctagat acgtttgact tcttaatggc aaaagactaa atgctgccca ctggcaagaa
     4681 gatgggaagg cctatcatgt acatataaca ttgtattcag gatatgaatt tatattataa
     4741 ataatgacaa taaaaaacat aaattcaaaa ccaaagaaca aaaagcctta ttttcagatt
     4801 atattaatca catagaagag tcttaaaaag cacattaaat aaatgtagaa agagctcagg
     4861 actcaagatc acaaacacac agtgaattgg aaaatcattg tcatctatca tatgaaaaat
     4921 tggaaataac agagagtaat tttaataagt ttttaaaata ttttataatg aaaactatat
     4981 tgtttaaaga aactaatgaa gtcttaaatc aatatgtata aaaagatcag atttactgat
     5041 agcgacatgc tattattcca aggtttgtgg gtatgtgtgt gtatatggta tgtatttgtg
     5101 tgtatctttg catgtgtgcg tggtgtttat gtgtgcatat ctgtgtctct gtatgtgtgt
     5161 ctgtatttct gtgtgtatgt ctgtgtgtgt ggtatgtgtc cgactgtcta tgtctgtgta
     5221 tgagacttac tgtgatgctt attgtttaca gggaatgttt ggtcttagac ctagaaaag
//
