LOCUS       NM_001368755           13182 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus helicase with zinc finger domain (Helz), transcript
            variant 2, mRNA.
ACCESSION   NM_001368755 XM_006534507
VERSION     NM_001368755.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 13182)
  AUTHORS   Bian,Y., Pang,P., Li,X., Yu,S., Wang,X., Liu,K., Ju,J., Wu,H.,
            Gao,Y., Liu,Q., Jia,Y., Qu,Z., Bi,X., Mei,Z., Yin,X., Wang,N.,
            Du,W. and Yang,B.
  TITLE     CircHelz activates NLRP3 inflammasome to promote myocardial injury
            by sponging miR-133a-3p in mouse ischemic heart
  JOURNAL   J Mol Cell Cardiol 158, 128-139 (2021)
   PUBMED   34043986
  REMARK    GeneRIF: CircHelz activates NLRP3 inflammasome to promote
            myocardial injury by sponging miR-133a-3p in mouse ischemic heart.
REFERENCE   3  (bases 1 to 13182)
  AUTHORS   Hasgall,P.A., Hoogewijs,D., Faza,M.B., Panse,V.G., Wenger,R.H. and
            Camenisch,G.
  TITLE     The putative RNA helicase HELZ promotes cell proliferation,
            translation initiation and ribosomal protein S6 phosphorylation
  JOURNAL   PLoS One 6 (7), e22107 (2011)
   PUBMED   21765940
  REMARK    GeneRIF: Downregulation of HELZ reduced translational initiation,
            resulting in the disassembly of polysomes, in a reduction of cell
            proliferation and hypophosphorylation of ribosomal protein S6.
REFERENCE   4  (bases 1 to 13182)
  AUTHORS   Diehl,F., Brown,M.A., van Amerongen,M.J., Novoyatleva,T.,
            Wietelmann,A., Harriss,J., Ferrazzi,F., Bottger,T., Harvey,R.P.,
            Tucker,P.W. and Engel,F.B.
  TITLE     Cardiac deletion of Smyd2 is dispensable for mouse heart
            development
  JOURNAL   PLoS One 5 (3), e9748 (2010)
   PUBMED   20305823
  REMARK    GeneRIF: Smyd2, similar to Smyd3, interacts with RNA Polymerase II
            as well as to the RNA helicase, HELZ
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 13182)
  AUTHORS   Corbo,J.C. and Cepko,C.L.
  TITLE     A hybrid photoreceptor expressing both rod and cone genes in a
            mouse model of enhanced S-cone syndrome
  JOURNAL   PLoS Genet 1 (2), e11 (2005)
   PUBMED   16110338
  REMARK    Erratum:[PLoS Genet. 2008 Jan;4(1):e18]
REFERENCE   6  (bases 1 to 13182)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 13182)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 13182)
  AUTHORS   Wagner,D.S., Gan,L. and Klein,W.H.
  TITLE     Identification of a differentially expressed RNA helicase by gene
            trapping
  JOURNAL   Biochem Biophys Res Commun 262 (3), 677-684 (1999)
   PUBMED   10471385
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645947.9.
            
            On Feb 22, 2019 this sequence version replaced XM_006534507.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.12311.1,
                                           SRR10223970.14821.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AL645947.9         22073-22121
            50-105              AL645947.9         23486-23541
            106-162             AL645947.9         29659-29715
            163-390             AL645947.9         49078-49305
            391-427             AL645947.9         52003-52039
            428-552             AL645947.9         66846-66970
            553-609             AL645947.9         69279-69335
            610-661             AL645947.9         73290-73341
            662-737             AL645947.9         74416-74491
            738-936             AL645947.9         76450-76648
            937-1044            AL645947.9         77076-77183
            1045-1342           AL645947.9         78206-78503
            1343-1610           AL645947.9         87991-88258
            1611-1944           AL645947.9         93123-93456
            1945-2075           AL645947.9         94224-94354
            2076-2255           AL645947.9         100675-100854
            2256-2357           AL645947.9         101493-101594
            2358-2536           AL645947.9         106312-106490
            2537-2655           AL645947.9         109120-109238
            2656-2801           AL645947.9         110306-110451
            2802-2949           AL645947.9         111920-112067
            2950-3133           AL645947.9         120190-120373
            3134-3362           AL645947.9         123252-123480
            3363-3567           AL645947.9         130184-130388
            3568-3619           AL645947.9         135235-135286
            3620-3810           AL645947.9         135982-136172
            3811-4018           AL645947.9         137748-137955
            4019-4098           AL645947.9         140139-140218
            4099-4735           AL645947.9         144175-144811
            4736-4970           AL645947.9         145187-145421
            4971-5487           AL645947.9         146669-147185
            5488-5740           AL645947.9         159431-159683
            5741-13182          AL645947.9         160525-167966
FEATURES             Location/Qualifiers
     source          1..13182
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 70.29 cM"
     gene            1..13182
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /note="helicase with zinc finger domain"
                     /db_xref="GeneID:78455"
                     /db_xref="MGI:MGI:1925705"
     exon            1..49
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            50..105
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            106..162
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            163..390
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     CDS             181..6075
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /note="isoform 2 is encoded by transcript variant 2;
                     probable helicase with zinc finger domain"
                     /codon_start=1
                     /product="probable helicase with zinc finger domain
                     isoform 2"
                     /protein_id="NP_001355684.1"
                     /db_xref="CCDS:CCDS88275.1"
                     /db_xref="GeneID:78455"
                     /db_xref="MGI:MGI:1925705"
                     /translation="MEDRRAERSCEQACASLQRQDYDMALQHCTDALLSLGQYSMADF
                     TGPCPVEVERIKIESLLYRIASFLQLKNYGQADEDCRHVLGEGLAKGERAFRAVLCCM
                     QLKGKLQLVSSILAKSLSGESLNGMVTKDLTRLKTLLTETETATSNVLSGCHVEDLDE
                     GSCNGWHFRPPPRGITSSEEYTLCKRFLEQGICRYGAQCTSAHSQEELAEWQKRYASR
                     LIKLKQQSENKQLSGSYMETLIEKWMSSLSPEKVLSECIEGVQVEHSPDLSVTVNTKK
                     SHQTWTFALTCKPARMLYRVALLYDAHRPHFSIIAISAGDSTTQVSQEVPENCQEWIG
                     GKMAQNGLDHYVYKVGIAFNTEIFGTFRQTIVFDFGLEPVLMQRVMIDAASTEDLEYL
                     MHAKRQLVTTAKRWDSSSKTIVDFEPNETTDLEKSLLIRYQIPLSADQLFTQSVLDKS
                     LTKTNYQARLHDLLYIEEIAQYKEVSRFNLKVQLQILASFMLTGVSGGAKYAQNGQLF
                     GRFKLTETLSEDTLAGRLVMTRVNAVYLLPVPKEKLVQSQGTKEKVYEATIEEKTKDY
                     VFLRISRECCEELSLRPDCDIQVELQFQLNRLPLCEMHYALDRIKDNAVLFPDISMTP
                     TIPWSPNRQWDEQLDPRLNAKQKEAVLAITTPLSIQLPPVLIIGPYGTGKTFTLAQAA
                     KHILQQQETRILICTHSNSAADLYIKDYLHPYVEAGNPQARPLRVYFRNRWVKTVHPV
                     VHQYCLISSTQSTFQMPQKEDILKHRVVVVTLSTSQYLCQLDLEPGFFTHVLLDEAAQ
                     AMECETIMPLALATKNTRIVLAGDHMQLSPFVYSEFARERNLHVSLLDRLYEHYPAEF
                     PCRILLCENYRSHEAIINYTSELFYEGKLMASGKQPAHKDFYPLTFFTARGEDVQEKN
                     STAFYNNAEVFEVVERVEELRRKWPVAWGKLDDGSIGVVTPYADQVFRIRAELRKKRL
                     SDVNVERVLNVQGKQFRVLFLSTVRTRHTCKHKQTPIKKKEQLLEDSTEDLDYGFLSN
                     YKLLNTAITRAQSLVAVVGDPVALCSIGRCRKFWERFIALCHENHSLHGITFEQIKAQ
                     LEALELKKTYVLNPLAPEFIPRALRLQHSGNSSRQQQSPPKVKSLYHPQSDHFQSDGI
                     VQPNPSVLIGNPIRAYTPPPPLGPHPNLGKSPSPVQRIDPHTGTSILYVPAVYGGNVV
                     MSVPLPVPWTGYQGRFAVDPRIITHQAAMAYNMNLLHTHGRGSPIPYGLGHHPPVSLG
                     QPQSQHAEKDQQEQNRNGKTDTNNPGPEINKIRTPEKKPTEPKQVDLESNPQNRSPES
                     RPGVVYSNTKFPRKDHLNPRHINNLPLPAPHAQYAIPSRHFHPLPQLPRPPFPASQPH
                     TLLNQQQNNLPEQPNQMAPQPNQVAPQPNQMTPQPNQVAPQPNQVVQQQSQAPPQAPQ
                     PAPQLSPAFQAGPTNAFFNNAVAHRPQSPAAEAVGPEQPPPPGLPDGHSPLRAITQPG
                     PILASPLNNFVDESSPGLPIEEALDGVHGSVALETLRQQQARLQQWSEHHAYLSQGGI
                     PYSHHHHPHLPHLPHTPIGLHQPPVRAEWKVAGRADDETETTFSRFQDLLRELSHRDQ
                     GDTGELAEMPPPQSRLLQYRQVQPRSPPAVPSPPSSTDHSSQFANFNDSSRDIEVANS
                     PAFPQRLPPQLFGSPFSLPSEHLAPPPLKYLAPEGAWNFANLQQNHLIGPGFPYGLPP
                     LPPRPPQNPFIHIQNHQHAAGQEPFHPLSSRTVSASSLPSLEEYEPRGPGRPLYQRRI
                     SSSSAQPCVEEASAPQDSLAQGKESQGHSNPPAFNFPAPESWANTTSSAPYQNIPCNG
                     SSRTSQPRELIAPPKTVKPPEDQLKPESGEVSSSFNYSMLQHLGQFPPLMPNKQIAES
                     ANCSSQQSPAGSKPAMSYASALRAPPKPRPPPEQAKKGSDPLSLLQELSLGSSPGSNG
                     FYSYFK"
     exon            391..427
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            428..552
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            553..609
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            610..661
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            662..737
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            738..936
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1044
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1045..1342
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1610
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1611..1944
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..2075
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2255
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2256..2357
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2536
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2537..2655
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..2801
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2949
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2950..3133
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3362
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3363..3567
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3568..3619
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3810
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3811..4018
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4019..4098
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4099..4735
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4736..4970
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4971..5487
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            5488..5740
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     exon            5741..13182
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6278..6283
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /note="hexamer: AATAAA"
     polyA_site      6299
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /note="major polyA site"
     regulatory      13159..13164
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
                     /note="hexamer: AATAAA"
     polyA_site      13182
                     /gene="Helz"
                     /gene_synonym="3110078M01Rik; 9430093I07Rik;
                     9630002H22Rik; DRHC"
ORIGIN      
        1 gtggggacgt tgacaagtgg atccaagatg gcgtagaaag taatgacagg aactggatga
       61 agtaattgat cactaatgaa ctacagagcc ggtgagatgt gcaagaaatt ataaagagct
      121 ctgatgggct atttgggtgt tacccagtgc agtgaactgc aggatttttg tccctgagtc
      181 atggaagaca gaagagctga aaggtcatgt gaacaagcgt gcgcgtcact gcagaggcag
      241 gactatgaca tggccctcca gcactgcacc gacgccctgc tctctcttgg ccagtactcc
      301 atggcagact tcacagggcc gtgcccggtg gaggttgagc gcatcaaaat tgagagtctt
      361 ctttacagaa ttgcctcatt cttgcaattg aaaaactacg ggcaagctga tgaagattgt
      421 aggcatgtgc tgggggaagg actggccaag ggagagcgtg ccttccgggc agtgctgtgc
      481 tgcatgcagc tgaaagggaa gctccagctt gtgtcctcca tccttgccaa gtcactgtca
      541 ggggagtctc tgaatgggat ggtaacgaaa gatttgacaa gactaaagac acttctcaca
      601 gaaacagaga cagcaacgag taatgtcctg tctggatgtc acgtagaaga cttagatgaa
      661 gggtcctgta atggttggca tttccgccca ccgcccaggg gcatcaccag cagtgaggag
      721 tacaccttgt gtaagaggtt tttagagcaa gggatctgta ggtacggtgc ccagtgtact
      781 tcagcacatt cccaggaaga gctagcagag tggcagaaaa gatacgcctc ccgactgatc
      841 aaactgaagc agcaaagtga gaataaacag ctctcaggca gctacatgga gaccttgata
      901 gagaagtgga tgagttcact gtctcctgag aaagtgctta gtgaatgcat agagggagtt
      961 caggtggagc acagccctga cctgtcggtc accgtcaaca ccaaaaaatc ccaccagacg
     1021 tggacctttg ctctgacctg taagcctgca agaatgctgt atcgggtagc cttgctttat
     1081 gacgctcatc gtcctcattt cagtatcatt gccatatctg ccggagatag tactacccag
     1141 gtatcacaag aagtccccga gaactgtcaa gaatggatag gaggaaagat ggcccaaaat
     1201 ggattagatc attacgtgta taaagtcggg atagcattta acacagaaat atttggaact
     1261 tttcgccaaa ccatagtttt cgactttgga ttggagccag tactcatgca gagggtgatg
     1321 attgacgcag cttctacaga agatcttgaa tacctgatgc atgcaaagcg gcagctggta
     1381 accacagcca agcgctggga ctcttcttct aagactattg tagattttga acctaatgaa
     1441 actactgatt tggagaagag ccttttgatc agataccaaa ttcctctgtc tgctgaccag
     1501 ctgtttacac agtccgtctt agacaaatca ttgaccaaga ctaactatca ggcacggctg
     1561 cacgacctgc tttatattga ggagatagcc cagtataaag aagtcagcag gttcaacctt
     1621 aaagtgcaat tgcagatttt agcaagcttc atgctcactg gagtttctgg gggtgcaaag
     1681 tatgctcaga atggacaact ttttggtcgt ttcaagctta ctgaaaccct ctcggaagac
     1741 actttggctg gacggctggt gatgaccagg gtcaatgctg tttatttatt accagtccct
     1801 aaagagaagc tcgttcagtc ccagggaacc aaagagaagg tttatgaagc tactattgaa
     1861 gaaaaaacaa aagactatgt atttttaagg atatccaggg aatgctgtga agagcttagt
     1921 ctgcggcctg actgtgacat ccaggttgag cttcagtttc agttgaaccg attacccctc
     1981 tgtgaaatgc attatgcact agacaggatt aaggacaatg ctgttttgtt tccagacatc
     2041 agtatgactc ctaccatacc ttggagtccc aacagacagt gggacgagca gttggatcct
     2101 cggctaaatg caaagcagaa agaagctgtt ctggccatca ccactccact ctccatccag
     2161 ctgccgcctg tcctcatcat tggaccctac gggacgggca aaaccttcac tctggctcag
     2221 gcggccaagc acatactgca gcagcaggag accaggattc tcatttgcac ccattctaat
     2281 agtgctgctg acctctacat aaaggattat ttacatccat atgtagaagc aggcaatccc
     2341 caggcaagac ctctcagggt gtatttccgg aatcgctggg tgaagactgt ccacccagtc
     2401 gtgcatcagt actgcttgat ctcgagcaca cagtccacct ttcagatgcc ccagaaggaa
     2461 gatattctta aacatcgagt ggtggtggtc acactgagca cttcccagta cctctgccag
     2521 ctggacctgg aacctgggtt cttcacgcac gttctcttag atgaagctgc ccaggccatg
     2581 gagtgtgaga ccattatgcc tctagcctta gctactaaaa acacacggat tgttttggct
     2641 ggtgatcaca tgcagctcag cccttttgtt tacagcgagt ttgccaggga gagaaacctt
     2701 cacgtttcgt tacttgaccg actctacgag cattaccctg ctgagtttcc atgcaggatc
     2761 ctcctctgtg agaactatcg ttcccatgaa gctatcatca actacacctc tgagcttttc
     2821 tatgagggga agctgatggc cagtgggaag cagccggcgc acaaggactt ctatccactc
     2881 acgttcttta cggcacgggg ggaggacgtc caagagaaga acagcacggc attctacaac
     2941 aatgcagagg tatttgaagt ggtggagcgt gtagaagagc taagacggaa gtggccagtg
     3001 gcatggggaa agttagatga cggcagcatc ggtgtggtga cgccatacgc tgaccaggtg
     3061 tttagaatcc gagctgagct tcggaagaag aggctctcgg atgtgaatgt ggaaagggtg
     3121 ctcaatgtcc aaggaaagca gttcagagtt ctgtttctta gcacagtgcg tacacgccac
     3181 acttgcaagc acaagcagac gccaattaaa aagaaagagc aactcctgga agactccacc
     3241 gaggacttag attatggttt tctatccaac tacaaacttc tcaatacggc catcaccaga
     3301 gcacagtcct tggttgctgt ggtgggtgac cctgttgctc tgtgctctat tggaaggtgt
     3361 agaaagtttt gggagcggtt tattgccctg tgtcatgaaa accacagctt gcacgggatc
     3421 acgttcgaac agatcaaggc acagttagag gctttagaac tcaagaagac ttacgtgttg
     3481 aatccactgg ctcctgagtt tatcccccgg gctttacgac tgcagcattc aggaaacagt
     3541 agcagacagc agcagtcacc acccaaggtg aaaagcctct atcatcccca gagtgatcat
     3601 ttccagagtg atggaattgt tcagcccaat ccttccgtac ttattggcaa tcctattaga
     3661 gcatatactc ctccgccccc tcttggaccg cacccaaact tgggaaaatc tccaagcccc
     3721 gttcaaagaa tagatcctca cactgggact agtattcttt acgtacctgc tgtctatgga
     3781 ggaaatgtag ttatgtcggt gcctttacct gtgccatgga cagggtacca gggtaggttt
     3841 gcagttgacc ctcggattat cacacaccag gcagcaatgg cctacaatat gaacctatta
     3901 cacacacacg gacgtgggtc accgatccct tatggtcttg gccatcaccc acctgtcagc
     3961 ctaggccagc cacagagtca gcacgcagag aaggatcagc aggagcaaaa ccgaaatggt
     4021 aaaactgata caaataaccc tggacctgaa attaataaga tccgaacacc agagaagaaa
     4081 cctacagagc ccaagcaggt tgatttggaa tcaaatccac agaacagaag tcctgaatcc
     4141 cgtcctggtg ttgtttattc caacactaaa tttcctcgaa aagatcatct caacccaaga
     4201 cacataaata atctccctct tcctgcacca cacgcccagt atgcaatccc tagccgccat
     4261 tttcacccgc tgcctcagct gcccaggccg ccgtttcccg cttcccagcc gcacaccttg
     4321 ttaaatcagc aacagaataa tttgcctgaa caaccaaatc agatggcacc tcagccaaat
     4381 caggtggcac cccagccaaa tcagatgaca ccccagccaa atcaggtggc accccagcca
     4441 aatcaggtgg tccagcagca gagccaggca cctccacagg ccccacagcc agcacctcaa
     4501 ctctctcctg catttcaggc ggggcccacc aatgcatttt tcaataatgc agttgctcac
     4561 cgaccacagt ctcctgctgc agaggctgtg ggtcctgagc agccgccccc tcccgggctg
     4621 cccgatggcc acagtccact gagagccatc acacagcctg gccccatcct ggcatcacct
     4681 ttgaacaact ttgttgatga gagctctcca ggattgccta tagaagaagc tttggatgga
     4741 gtgcatggaa gcgtagctct ggagacgctg aggcagcagc aggcgcggct acagcagtgg
     4801 agtgagcacc atgcctacct gagccagggc ggcatcccct attcacacca tcaccatcct
     4861 cacctcccgc atcttcccca cacacccatt ggcctccacc agccaccagt gagggcagag
     4921 tggaaagtcg ccggcagggc tgacgatgaa acagagacaa cattctcaag gtttcaagac
     4981 ttactcagag agctgtccca tcgggatcaa ggtgacactg gagaactagc tgaaatgcca
     5041 ccacctcagt caagacttct gcagtacaga caagttcagc ctagaagtcc gccagcggtc
     5101 ccgtctcccc cttcctccac agaccacagt agccagtttg ctaactttaa tgacagcagc
     5161 agagacattg aagtagccaa cagcccagca ttcccgcagc gcctcccgcc tcagctattt
     5221 ggctcccctt tctcgttgcc atctgaacac cttgcccctc ctcctctgaa atacctggct
     5281 ccggaaggag catggaattt tgctaacttg cagcagaatc acttaatagg gccgggcttt
     5341 ccctatggcc tacctccatt gcctcccagg ccaccacaga acccttttat acacatccag
     5401 aaccatcaac acgctgctgg tcaagagcca tttcacccac tgtcatctcg aacagtgtct
     5461 gcttcttcgc tccccagcct agaagagtat gagcccagag gacctggtcg gcccttgtac
     5521 caaagaagaa tctcatctag ctcagcccag ccttgtgtgg aagaggcaag cgctccccaa
     5581 gacagcctgg ctcagggcaa agaatcgcag ggccacagca acccacctgc cttcaacttc
     5641 ccggcccccg agtcctgggc caacaccacc tcatctgccc cctaccagaa cattccgtgc
     5701 aatggatcca gcaggacgag tcagcccaga gagttgatag ctccacccaa gactgtcaaa
     5761 ccccctgagg atcagctgaa gcccgagagc ggggaggtgt ccagttcctt caactactcg
     5821 atgctgcagc accttggcca gttcccaccc ctcatgccca acaagcagat cgcagagtcc
     5881 gccaactgca gcagccagca gagcccggca gggagcaagc ccgccatgtc ctacgccagt
     5941 gcgctgcggg ccccgcccaa gcccaggccg ccacccgagc aggccaagaa gggcagcgac
     6001 ccgctgtccc tgctccagga gctcagtctg ggcagcagcc cgggcagcaa tggcttctac
     6061 tcctacttca agtgaccccg ctcgcgctgt cctcagagaa tatgcggttc ctgtccagtc
     6121 tgcaggatgg aggtggcggc tctgtgtgcg tgcagccctc cattacgttc actgcgctct
     6181 cagccccctt gctgctagac ctgcgcctac gtttaaaagt atattccatt attttgcatt
     6241 tttgatgtga gtcagaattt tgacagcttt tatgtagaat aaaaatattt ttaaatttgt
     6301 gtattgttac atatgtttgc atcaagctag cagctaagag gttaactgtg caactataaa
     6361 aaaaagaaaa aaaagtttgt ctaaaatgta tttaaaaaga aaaaaattgt taagtagcat
     6421 ttacatttat tcaataatat taccacatgc tgggtttctg taccagaaat ggccagttga
     6481 tgtacaaatg tatgttattt ttgcttaaat tcagttaaaa atttaaaaaa aaaaacaaag
     6541 gggcagcatc ttctaagacc ccctcccccc ttttttgtga tcacaggatg ctgcgttcta
     6601 aaactttccg agttttagac gatctgtgac gtgctcttct ctccatcagc ataggggagg
     6661 ccaaggctgc ctttgcccga gtattctgca tgccaccact gggttttgaa atgtgaaaaa
     6721 gaaaaaagga aaaaaaaaaa aaaagcaacc ctggtggcat agtcagtttt ctttgttttg
     6781 tttttgacaa gcaagacttc cagcttgtct ttaacattga ggtgagaggg tttggtttgg
     6841 tttaaacaaa tggggaagct gaagcatttt taggattgtt ggtgcttagt agacttgata
     6901 actattttaa aactgcgtga atttagtgaa aaatattttt tctcactctg catgtgtaag
     6961 tgttcgtagc ctggccccct cctccagggc agaaagacct gtgccaacta acggtttggg
     7021 ttgcttttcc tccattaaca atgttggtac cttattgatg tttacgttaa gatgatgtga
     7081 cctacacaga gcaattctaa gatctttcta agatgttaca tacacagagc aattctaaga
     7141 tctttctaat tgcttgtttg agggatatca ttaaaggaaa aaaaaaacat tgtcaaaaat
     7201 tgatgaaaat ctagggtatg tttcagatcc tttgaatcat ctgaaattcc ccccttgaac
     7261 tcattagaaa tatgaaattt aagcaactgt ccgcagagat ccacagcctg ccagttgttt
     7321 tgaatgaacg ttgaaggaag aggttgtcct cacgtgttaa cacatccgaa acaatgaatg
     7381 tcctttgtca tttcctgatc ccgtagctct aattttgtat ttctgagaaa tcttgagact
     7441 acaatctatc tttttttttt ttttttcctt tttaaactaa gatgaggcca gggggatatt
     7501 tcaaaagagg ccgactttcc tgccgatcac acatactttt ctggaagact tttgatagac
     7561 cattttaaga tgggttggat gtggcaaggg ggcagcagaa ttagtctgtg tgactagagg
     7621 ttagtctatt gcagccagcc acagccccct gtgtgggcta cacaggctgc gattaaaggc
     7681 tacgttggtt aaggggcagc aaactcctgc actgatgata cagcctctca gattcagttc
     7741 tgcctctgtg ctcaggactg ggctactgga acctggcttc ctgttgttcc tgtctcgggc
     7801 tacatgcctc ttctgagctg ccaggtatgg acggacccac agactaaagt ggtgttcaca
     7861 gatcccggca ggcacgggga tccactgagt caggacacca cattctgcgt gacatgacat
     7921 catcacatgc tggcgtgacg caggcagaat gagacaactt ttgccacagg tctctgtttg
     7981 ctaactctgt gcccaagtag gactcaccca tgatagagct gtctgtgtcc cataggtgag
     8041 tgcccccacg cccacgcatg cagacacaca ggacaaagta catgccgggc aagagttcag
     8101 agccatcctg ctaattcaga cagagttata ctgtcagttt tcgggtcacc tctcgccagg
     8161 agatcattgc ctactttttt gtttttcata cttgaacctt taaaaatata tgagtttttt
     8221 aaattattta tatttagtta aagtcacgtg tgcaccctat catcttatat aagacactga
     8281 tggtgggacc ccggggatgg ggaacccgtc aggttgcggt gacattctca acgtggttat
     8341 gtctaagaaa tgacactagc tacttcctta cttgactgcg ctgttgcgag tgtcggtgcg
     8401 ttcccatctc tctcctgctt ctgcagttgg gggtttcctt ccgtcgcttc acggtctggt
     8461 tagagtactt atccctcctt cccagcgtta ctcttgtctt cccgtccagg ctctaaaaag
     8521 cctatttctt acacccagca cctcgtaaca gtcgacagca gaccttacca gaagagccat
     8581 tgcgagctct cgcttggggc tctctgcagt caccccggta gcattagcag ggttagtcag
     8641 cctcccggaa atagcttgct tctttcattc aactttagag ttcagatggg acatgtgtac
     8701 atacgtgtgt atatacgtgt ttgtttacat acataagcac acatttatta tttttttaag
     8761 gaaaacttga taggtctgcc atgccttttc ttctcctgag tttcctttat ggtttcttct
     8821 cttctcttca tcctttcccg tcggaggcgt ttccttgtcc ttgccagtac tggttttagc
     8881 cattgcagta caaactcgag gtgtttcttt cttccctttc tgtccctctc tccttcatct
     8941 ttctcttcca gttcttttag gtgataattc agagtattcg cgctaagttt gttcccctcc
     9001 cccacccccc caccctttat ctcttgatct aaaaataaat tcctgatgag aattgacaag
     9061 cctcattcac tgtatcatgg gtattttctg gtgtgttttt ttcaaagaag tccattgtgg
     9121 ccccacgctt gctagtcacg ttatctcgtc cttgaccagt cagtcgttag ttattgatca
     9181 tggagactaa ggatctcggt cttcctgtcg tcacacagac atgctcgctg tctgggtcat
     9241 cttgtgtgag gagaacaaaa ttgtctgctg tgggttcttt ttcttttgcc ttgtttctaa
     9301 tcaagatcgt aggatgagtt caggtttgat gtaagcatca actggtcctt tctgttcaga
     9361 gaacttcagg gtaaggaaaa tacgcggaag tctcccagag taagcattcc ccagtaaaca
     9421 tggcggtctc tggagcctat gcattggaga ggttttcctc tttctgaagg ccttgaggtt
     9481 ctgaggaaac tgtcttttta atcttgagct catatgctgt gttatgttgc tgtagtgtag
     9541 ttaatggttc taagtttggt atgtttcatt ttgagagcaa agctgaaaac ataaaagaat
     9601 gtatctctct agccaccgcg actgcgagtc agggtgtgga gaacaatttt aggctaaatc
     9661 tgaacattcc cacgattgtc atgggaaatg ttagatttcc tgaactaggt gattttttgt
     9721 ttgtttgttt gtttttttaa ggagatgctc atgagcgaaa gaatcattgc gtagtggaga
     9781 gttttattag tagaaaaatt atgtagaaac ataagtttaa aacttcccat agagtaaaac
     9841 atacacatta ctgctttaaa atattaccta ggctgtccat ccctccccca cccacctctc
     9901 cacctcctca cacttaaacc cgccttttct cagtgagtag atccgggttt gtttgtttgt
     9961 ttgttattct tgcctttccc ataatgtttg acaaccctgc tcccaaccgc cccatgtgtc
    10021 ctgctcacat ctgggcgcag gtgtcatctg atcagtattc acactgccca gaagttagag
    10081 aagaggcagg tggtgcttgc tctccttgtt cagagcatca cttggtgtgt ccctggtagc
    10141 ctggtaccca gagtacccgt catacgggtc ctcagagcat ccactccatc aagccttcac
    10201 atttccctct gcgacttgac agaggcttcg aggttcacac ttgccagttt tatccctcgt
    10261 ttaactagca attccaagac cacagaagga ggggattttt actgaagttt agcatcccaa
    10321 taaaacatca gaaagtggca ttatatcatc aactctaagc tagaatatca ggtcaaacaa
    10381 tttttatgtt taaagtttta gtgtaacatt cagatatttc gaacctactg aaaatatcta
    10441 cttaacaaaa cctgcattgt ttaaaaaaga aaaaaagaaa aagaaaaaaa ctttatgtca
    10501 ggaggtacat aatagaagac tctagactta gacttgtgag ctgtggttct aatccagccc
    10561 atttgtccag tggtaagctt gaagcttctg aaatgagttt caagagagcc tacaagtctt
    10621 atagtttcag tcatcagagt ggtagaccag actttacttg caatttgttc aacagagaga
    10681 ttttattttg atacaaatat taaacataat gcatagtaag ttttatataa ttctatgagt
    10741 tcattaaaca atattatgag attttcagta ttttgggggg ggcgtctttt tggctatttc
    10801 tacctcatat gaggtctctt ctgtatttta tttagtatac tagcatgtgc ttgcctgcct
    10861 tagaatgatg tacgggaggg gaaatcaata ttacctttta agaaaaattt tgatttttat
    10921 ggaataattg ttttaatatt ttaatgtttg aaactgaggg gggtaagggt ggcagataac
    10981 atacagtggt aactatttac aaacctatca tattttaaga ttttagaatt gtgagcaaga
    11041 tcaagaaaaa taaaggggga aaaaaaagtg attatgaagc tgaagctggc ctcagttttc
    11101 cctggacggg ttgttgagat tgctgggccc actgccaggg tggcgtcaca gccgtccagg
    11161 aagcgcactt tgagagtcag gaggggttta tctctttact cgtgggccat tttaatcatc
    11221 ctggcacctc aagtggcccg ttcgtgggaa gagctctctc tccctttggg gtctctgcta
    11281 ctcctcatta gggtgcaggg gcgctttcat ttctggagtg agtttgttgt tagtgacgca
    11341 gctgcctggc tattgttcgg aagctgtgcg tttgtgggat gtgcttagag tcgacctcga
    11401 tccctccaca tggagacata attgtggaaa gaagccatcg gatgccctga gagtgtgctt
    11461 gcacttgccc aggctcttag tcccggtcct tcggtggcag tgacttgggg catcggaaac
    11521 attagacagt ccccaatgaa tggagcctat agtttttaat cttatccccc cctttcttat
    11581 tttgtatgtg tgttggggcg agggcaaagg atttaccttc gtgttatatg tttctcaggt
    11641 taacaaagtt attttgggtt ctctgccgtg cctgcaggtg ttagggagag cctgggtgtt
    11701 gtatgtggca ggaaaaaagc aattgtttct gactccttag ctaccttgaa gacctctgta
    11761 tgtttgccga agagttgagg ggacacacca cccgcatagc atgagtgact cctaagtcag
    11821 agcagacatg ggtgagcacg ttagttttga gaaagaaact gcctacaaca taatcaacct
    11881 gacaggtctt tggatagttt tacatttgtg tgttgtgttt aatgttcagc ctgggctgca
    11941 gcttggcggg tatcgggttc tgctttctgg tttctagtct ggctttgaat ttactgggtt
    12001 gctagcaaga aaacaaatct atttttaagg gaatagaatg tagaacggcg taggcgtaga
    12061 agtataatac acagtgacca tcatagattt acacagcttt agagttctta gcccccagga
    12121 cttcctacat aaaaggcccg ggaagctcta gctgcaggtt gctggtgtgc tcagcacagc
    12181 tttcctttat gcactcccag cttataaggg acttcggttt atttgcgatt tgccttgcat
    12241 gataccactt tgtatctttc aggaggtcag aggactcttt tgtccaggtt gaagtcccaa
    12301 agtgtcagtt ttacctaaga ttgtttctca acgacccccc ctccccccgc cgccgccgaa
    12361 gggtccctga tttccatgta tcaggaaggt gactgcttct atctatccat gtatatgaca
    12421 ttatgtacat caagtactga atctgatttc tgtgccagcc atctgacctg attctttgag
    12481 acccataaag gtactggtta taggtaatag ttgcatcccg ggagaataga gtggtccaaa
    12541 tactttctct gaattataaa gctaatgggg tttgcatttt agtaaagcgt cctaattgtc
    12601 agtgatgcag gatcttgctt tttaattttt tttttttaaa tacaattctc taaatgtggt
    12661 cagtaatgta tgggatcagt cacatagtag atgcattctg gatcttctat tgggaaagcc
    12721 ttcataaggg aggccatatg taaaaccacc ctggtccgtt taccttacaa gcaccactta
    12781 tgcagcatct gactcagcat aaatctatgg caaaaccaca acagtgactc tgtccctttg
    12841 cggtatacca tgcctcgtct gagaggctct aataccggaa gagtttattc ttaaaagtaa
    12901 agatggtttt gtatcataag ataaccccca aagctggcca ccaaggcttc ctttctctgt
    12961 tcttcctctt tcctaactcg ggttttggag tctccttagc ttacgctact ttcttaggac
    13021 tttggttttt gtttttttgt ttgttttttt ttgagcccag gagtctacag tgtgaccctt
    13081 gtcagtgaag ttcttttgtt ttaaaagatt catggtaaca caaaatgtat tttactgcta
    13141 caactaaaat gtatttataa taaaaaaatg cctttttaat aa
//
