LOCUS       NM_001081107            3688 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus helicase, POLQ-like (Helq), transcript variant 1,
            mRNA.
ACCESSION   NM_001081107 XM_132236
VERSION     NM_001081107.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Wan,C., Huang,Y., Xue,X., Chang,G., Wang,M., Zhao,X.Y., Luo,F. and
            Tang,Z.Z.
  TITLE     HELQ deficiency impairs the induction of primordial germ cell-like
            cells
  JOURNAL   FEBS Open Bio 14 (7), 1087-1100 (2024)
   PUBMED   38720471
  REMARK    GeneRIF: HELQ deficiency impairs the induction of primordial germ
            cell-like cells.
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Bakhshalizadeh,S., Bird,A.D., Sreenivasan,R., Bell,K.M.,
            Robevska,G., van den Bergen,J., Asghari-Jafarabadi,M., Kueh,A.J.,
            Touraine,P., Lokchine,A., Jaillard,S., Ayers,K.L., Wilhelm,D.,
            Sinclair,A.H. and Tucker,E.J.
  TITLE     A Human Homozygous HELQ Missense Variant Does Not Cause Premature
            Ovarian Insufficiency in a Mouse Model
  JOURNAL   Genes (Basel) 15 (3), 333 (2024)
   PUBMED   38540391
  REMARK    GeneRIF: A Human Homozygous HELQ Missense Variant Does Not Cause
            Premature Ovarian Insufficiency in a Mouse Model.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3688)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3688)
  AUTHORS   Zhao,J., Lu,P., Wan,C., Huang,Y., Cui,M., Yang,X., Hu,Y., Zheng,Y.,
            Dong,J., Wang,M., Zhang,S., Liu,Z., Bian,S., Wang,X., Wang,R.,
            Ren,S., Wang,D., Yao,Z., Chang,G., Tang,F. and Zhao,X.Y.
  TITLE     Cell-fate transition and determination analysis of mouse male germ
            cells throughout development
  JOURNAL   Nat Commun 12 (1), 6839 (2021)
   PUBMED   34824237
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3688)
  AUTHORS   Murtaza,G., Yang,L., Khan,I., Unar,A., Khan,M., Huan,Z., Khan,R.
            and Shi,Q.
  TITLE     Identification and Functional Investigation of Novel Heterozygous
            HELQ Mutations in Patients with Sertoli Cell-only Syndrome
  JOURNAL   Genet Test Mol Biomarkers 25 (10), 654-659 (2021)
   PUBMED   34672775
  REMARK    GeneRIF: Identification and Functional Investigation of Novel
            Heterozygous HELQ Mutations in Patients with Sertoli Cell-only
            Syndrome.
REFERENCE   6  (bases 1 to 3688)
  AUTHORS   Luebben,S.W., Kawabata,T., Akre,M.K., Lee,W.L., Johnson,C.S.,
            O'Sullivan,M.G. and Shima,N.
  TITLE     Helq acts in parallel to Fancc to suppress replication-associated
            genome instability
  JOURNAL   Nucleic Acids Res 41 (22), 10283-10297 (2013)
   PUBMED   24005041
  REMARK    GeneRIF: The findings suggest that HELQ contributes to genome
            stability in unchallenged conditions through a mechanism distinct
            from the function of FANCC.
REFERENCE   7  (bases 1 to 3688)
  AUTHORS   Adelman,C.A., Lolo,R.L., Birkbak,N.J., Murina,O., Matsuzaki,K.,
            Horejsi,Z., Parmar,K., Borel,V., Skehel,J.M., Stamp,G.,
            D'Andrea,A., Sartori,A.A., Swanton,C. and Boulton,S.J.
  TITLE     HELQ promotes RAD51 paralogue-dependent repair to avert germ cell
            loss and tumorigenesis
  JOURNAL   Nature 502 (7471), 381-384 (2013)
   PUBMED   24005329
  REMARK    GeneRIF: results reveal a critical role for HELQ in
            replication-coupled DNA repair, germ cell maintenance and tumour
            suppression in mammals
REFERENCE   8  (bases 1 to 3688)
  AUTHORS   Marini,F., Kim,N., Schuffert,A. and Wood,R.D.
  TITLE     POLN, a nuclear PolA family DNA polymerase homologous to the DNA
            cross-link sensitivity protein Mus308
  JOURNAL   J Biol Chem 278 (34), 32014-32019 (2003)
   PUBMED   12794064
  REMARK    GeneRIF: identification of homolog to POLN
REFERENCE   9  (bases 1 to 3688)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 3688)
  AUTHORS   Marini,F. and Wood,R.D.
  TITLE     A human DNA helicase homologous to the DNA cross-link sensitivity
            protein Mus308
  JOURNAL   J Biol Chem 277 (10), 8716-8723 (2002)
   PUBMED   11751861
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161510.10.
            
            On Feb 6, 2007 this sequence version replaced XM_132236.8.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC082601.1, SRR17253014.1204720.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-307               AC161510.10        47173-47479
            308-1016            AC161510.10        48711-49419
            1017-1195           AC161510.10        52931-53109
            1196-1396           AC161510.10        53796-53996
            1397-1469           AC161510.10        54364-54436
            1470-1567           AC161510.10        55537-55634
            1568-1666           AC161510.10        58691-58789
            1667-1812           AC161510.10        60346-60491
            1813-2052           AC161510.10        62576-62815
            2053-2194           AC161510.10        66480-66621
            2195-2299           AC161510.10        66937-67041
            2300-2519           AC161510.10        71609-71828
            2520-2677           AC161510.10        73882-74039
            2678-2776           AC161510.10        75277-75375
            2777-2950           AC161510.10        77318-77491
            2951-3064           AC161510.10        78972-79085
            3065-3199           AC161510.10        80698-80832
            3200-3688           AC161510.10        83137-83625
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 48.52 cM"
     gene            1..3688
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /note="helicase, POLQ-like"
                     /db_xref="GeneID:191578"
                     /db_xref="MGI:MGI:2176740"
     exon            1..307
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..3337
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /EC_number="3.6.4.12"
                     /note="isoform 1 is encoded by transcript variant 1; DNA
                     helicase Hel308; POLQ-like helicase; mus308-like helicase;
                     helicase, mus308-like"
                     /codon_start=1
                     /product="helicase POLQ-like isoform 1"
                     /protein_id="NP_001074576.1"
                     /db_xref="CCDS:CCDS39184.1"
                     /db_xref="GeneID:191578"
                     /db_xref="MGI:MGI:2176740"
                     /translation="MEDGCPRIRRRVSVRKRNRGNLENLRASPTPAELQPAEDTEDEA
                     AAGSRRRKTGSPEHAQENDSEEDMFGDYDSFTESSFLAHVDDLEQRYMQLPECGDRDA
                     DSGTKDLCSAGLKNNLRVTTVINLTDPETSEHGQKQSHLDVPAEPEPGSDLSFDVPSS
                     QILYFENPQNSPEALGDPCTKKTNGDPQKSSHEELVSSHTEQPEPNNDFSNVRAASES
                     SRRKSLKDHLKSTMAGNARAQTPAFPRSKHLREALLSEEISVAKKAIESPSDDLGPFY
                     SLPSKVRDLYVQLKGIKKLYDWQHTCLTLRSVQERKNLIYSLPTSGGKTLVAEILMLQ
                     ELLCRQKDVLMILPYVAIVQEKISSLSSFGIELGFFVEEYAGSKGRFPPIKRREKKSL
                     YIATIEKAHSLVNALIETSRLSTLGLVVVDELHMIGEGSRGAILEMTLAKVLYTSKTT
                     QIIGMSATLNNVEDLQAFLKAEYYTSQFRPVELKEFLKVNDTIYEVDSQAADGMTFSR
                     LLSYKYSEALKKMDPDRLVALVTEVIPNYSCLVFCPSKKNCENVAEMLCKFLSKDYLN
                     HREKEKCEVIKSLRNIGNGKVCPVLKRTVPFGIAYHHSGLTSEERKLLEEAYSTGVLC
                     LLTCTSTLAAGVNLPARRVILRAPYVANTFLKRNQYKQMVGRAGRAGIDTAGESILLL
                     QEKDKQQVLELISGPLETCCSHLVEEFTKGIQALFLSLIGLKIAASLGDIYQFMSGTF
                     FGVQQKILLKEKSLWEITVDALEHLTEKGLLQKDSCGDNEGLECHFRITKLGQASFKG
                     AIDLAYCDTLYRDLKKGLEGLVLESLLHLIYLTTPYDLAAQSEPDWMVYFKQFGQLSP
                     TEQNVAALLGVSESFIGKKAAGQAVRKKVDKNVVNRLYLSFVLYSLLKETNVWSVSEK
                     FNLPRGYIQNLLMGAASFSSCVLHFCEELEEFWVYKALLVELTKKLTYCVKAELIPLM
                     EVTGVLEGRAKQLYNAGYRSIMHLANANPEVLVKTIDHLSRRQARQIVSSAKMLLHEK
                     AEALQGEAEELLRLPADLPGLGGPSSERAGSHAGDVTLS"
     exon            308..1016
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1195
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1396
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1469
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..1567
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1666
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1812
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            1813..2052
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2194
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2299
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2300..2519
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2677
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2776
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2950
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            2951..3064
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3199
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     exon            3200..3688
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3661..3666
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /note="hexamer: ATTAAA"
     polyA_site      3685
                     /gene="Helq"
                     /gene_synonym="D430018E21Rik; Hel308"
                     /note="major polyA site"
ORIGIN      
        1 acgtgacccg gggctgcgct gctgagggat ggaaagctga tgctgggtca cctgctgtct
       61 ccagaacggc gccaccggct ccgggctgca cccagggcgg ctgtcgcagg gactctgggc
      121 cgttgtcatg gaggacggct gtccccgcat ccgccggcgg gtgtctgtcc gcaaaaggaa
      181 ccgcggcaac ctggagaacc tccgtgcctc cccgaccccg gccgagctcc agcctgccga
      241 ggacacggag gacgaagctg cagccgggag ccgccgccgg aaaaccggga gcccggagca
      301 cgcccaggaa aatgacagcg aagaggacat gtttggtgac tatgacagct ttacagaaag
      361 ttccttctta gctcacgttg atgacctgga acagagatat atgcaactgc ctgaatgtgg
      421 ggaccgtgat gcagactctg gcaccaaaga cctttgttca gcaggcctca aaaacaacct
      481 cagagttact actgtaatca acctcactga cccggaaact agtgagcacg gccagaagca
      541 gagtcaccta gatgtccctg ctgaaccaga acctggaagc gatctttcgt ttgatgtgcc
      601 ttcttctcaa attttatact ttgaaaatcc gcagaactca ccagaagctt tgggcgatcc
      661 gtgcactaag aagacaaatg gggacccaca gaagtcatca catgaagagt tggtctccag
      721 ccacacagag caacccgagc caaataatga cttctctaac gtcagagctg cttcagagtc
      781 aagcaggaga aaaagtctga aagaccatct gaaaagtacc atggctggga atgccagggc
      841 ccagacccca gcatttccca ggagtaaaca cctcagagag gcgctcctgt ctgaggagat
      901 cagcgtcgcc aagaaagcca ttgagtcacc gtcagatgat cttggtcctt tttattcgct
      961 acccagcaaa gtgagagatc tttacgttca attgaaagga atcaaaaagc tatatgactg
     1021 gcaacatact tgcttaacgc taagatctgt gcaagaaaga aaaaatttaa tatattcctt
     1081 gccaacgagt ggcgggaaaa cccttgtggc tgagatctta atgctgcaag agctgctctg
     1141 ccgacagaaa gatgtcttaa tgatccttcc gtatgtggcc attgtccaag agaagatttc
     1201 cagtttatcg agttttggta tagaacttgg tttctttgtt gaagaatatg ctggaagcaa
     1261 aggaagattt cctccaatta aaaggaggga gaagaaatca ctgtatatcg ccactatcga
     1321 gaaagcgcat agcctggtga atgccttgat cgaaaccagc aggctcagca ctctgggtct
     1381 ggttgtcgtc gatgagttgc acatgattgg tgaagggagc cgtggcgcca tactggagat
     1441 gaccctggca aaagtcctct acaccagcaa aacaactcag attatcggca tgagtgcaac
     1501 attgaacaac gtagaagacc tgcaggcgtt ccttaaagcc gagtactaca ccagtcagtt
     1561 cagaccagtg gaattaaaag aattcctgaa agtaaatgac acaatctatg aggtagacag
     1621 ccaggctgcg gacggcatga ccttttcacg gctcctgagt tataagtact ctgaggccct
     1681 gaagaagatg gatcccgacc gcttggtagc actggtgaca gaagttattc ctaactactc
     1741 ctgcctggtg ttttgtccca gtaagaagaa ctgtgaaaat gtagcagaaa tgttgtgcaa
     1801 gtttttaagc aaggactatc taaaccacag agagaaagaa aaatgcgaag tgattaaaag
     1861 tttgaggaat atcggcaatg gtaaagtgtg tcctgtcctg aagcgcaccg tccctttcgg
     1921 catcgcctat caccacagcg gtttaactag cgaagagcgg aagcttctgg aagaggctta
     1981 ctccacaggc gtgctctgtc tcttgacctg cacgtccacc ctggcagcag gggtcaatct
     2041 gcctgcacgg agagtcatct tacgagctcc ctatgtggct aacacgttct taaagaggaa
     2101 tcagtacaag cagatggttg gcagggccgg ccgcgctggg atagacactg ccggggagag
     2161 catccttctg ctgcaagaga aggacaagca gcaggtcctg gaactcataa gcggacccct
     2221 ggagacctgc tgcagccatc ttgtcgagga attcaccaag gggatccagg ctttgtttct
     2281 ctctttaatt ggtctgaaga tcgcagcaag tcttggtgac atatatcagt ttatgagtgg
     2341 tacatttttt ggtgttcagc aaaagatttt attgaaagaa aaaagtctct gggaaataac
     2401 tgttgacgct cttgaacacc tgacagagaa aggacttcta cagaaagaca gctgtggcga
     2461 caatgagggg ttagagtgtc acttccggat tacgaaactg ggccaagctt cttttaaggg
     2521 agccattgat ttggcctatt gtgacactct gtacagagac ttgaagaaag gtctggaagg
     2581 acttgtactg gagagccttc ttcatctgat ctacctaacc acgccctatg acctggctgc
     2641 tcagtctgag cctgactgga tggtgtactt caagcagttt ggtcaactca gcccaacaga
     2701 gcagaatgta gccgctcttc tcggagtctc cgaaagcttt atcgggaaga aagcagcagg
     2761 acaagccgtc agaaagaagg tggacaagaa cgttgtcaac agactctacc tgtcgtttgt
     2821 gctttattct ttgctgaaag agaccaatgt ttggagtgtg tctgagaagt ttaacctgcc
     2881 ccgaggatac atacagaacc tgcttatggg agctgcctcc ttctcttcct gcgtgttaca
     2941 tttctgtgag gagctggagg aattttgggt ttacaaagcc cttttggtgg aactcaccaa
     3001 gaagctgacg tactgcgtga aggcggagct aatccctctc atggaagtga ccggggtctt
     3061 agagggtcga gcaaaacagc tatataacgc aggttacagg agcatcatgc acttagccaa
     3121 tgccaaccca gaagtgttgg tgaagacaat tgatcatctg tccagacggc aagccaggca
     3181 gattgtttcc tcagccaaga tgctgttgca tgagaaggcg gaggcactgc aaggagaagc
     3241 agaagaatta ctgagattgc ctgcagacct ccctgggctt gggggtccca gctctgaaag
     3301 ggcaggaagc catgctgggg acgtaactct gagctagggc atttgcctga gatgcctccg
     3361 gccctggaat aaatcctcag tacctccaag acagaagaaa aggccggggg gttgtaaact
     3421 gcccagtggt gagcacctgt aacgcagcac tcgggagaca ggacggacaa tgagtgtcag
     3481 aaatcagctt cagctacact gagagttgga ggctagcctg ggctgcatga gtccctgtct
     3541 ccaaaagaaa aaaccccatg aagctgtctg atgtatgttt tttaatttga cttatttatt
     3601 atatatatat tttattattg aaaatccccc gtgtaaatga aatgtgtatt ttaattatac
     3661 attaaaagct gtggatgagc ttttagaa
//
