LOCUS       NM_001001883           11179 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein
            ligase 2 (Hecw2), transcript variant 1, mRNA.
ACCESSION   NM_001001883 XM_355199
VERSION     NM_001001883.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Li,L., Ruan,X., Wen,C., Chen,P., Liu,W., Zhu,L., Xiang,P.,
            Zhang,X., Wei,Q., Hou,L., Yin,B., Yuan,J., Qiang,B., Shu,P. and
            Peng,X.
  TITLE     The COMPASS Family Protein ASH2L Mediates Corticogenesis via
            Transcriptional Regulation of Wnt Signaling
  JOURNAL   Cell Rep 28 (3), 698-711 (2019)
   PUBMED   31315048
REFERENCE   3  (bases 1 to 11179)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11179)
  AUTHORS   Yang,L., Han,B., Zhang,Y., Bai,Y., Chao,J., Hu,G. and Yao,H.
  TITLE     Engagement of circular RNA HECW2 in the nonautophagic role of ATG5
            implicated in the endothelial-mesenchymal transition
  JOURNAL   Autophagy 14 (3), 404-418 (2018)
   PUBMED   29260931
  REMARK    GeneRIF: that circHECW2 regulated the EndoMT by directly binding to
            MIR30D, a significantly downregulated miRNA from miRNA profiling,
            which subsequently caused an increased expression of ATG5
            Erratum:[Autophagy. 2022 Jan;18(1):234. doi:
            10.1080/15548627.2020.1751979.. PMID: 32286128]
REFERENCE   5  (bases 1 to 11179)
  AUTHORS   Qiu,X., Wei,R., Li,Y., Zhu,Q., Xiong,C., Chen,Y., Zhang,Y., Lu,K.,
            He,F. and Zhang,L.
  TITLE     NEDL2 regulates enteric nervous system and kidney development in
            its Nedd8 ligase activity-dependent manner
  JOURNAL   Oncotarget 7 (21), 31440-31453 (2016)
   PUBMED   27119228
  REMARK    GeneRIF: Data show that NEDL2 regulates GDNF/Ret/Akt pathway
            depends on its Nedd8 ligase activity rather than ubiquitin ligase
            activity.
REFERENCE   6  (bases 1 to 11179)
  AUTHORS   Wei,R., Qiu,X., Wang,S., Li,Y., Wang,Y., Lu,K., Fu,Y., Xing,G.,
            He,F. and Zhang,L.
  TITLE     NEDL2 is an essential regulator of enteric neural development and
            GDNF/Ret signaling
  JOURNAL   Cell Signal 27 (3), 578-586 (2015)
   PUBMED   25555806
  REMARK    GeneRIF: NEDL2 positively regulates enteric neural precursor
            proliferation through the GDNF/Akt signaling pathway.
REFERENCE   7  (bases 1 to 11179)
  AUTHORS   Dinudom,A., Fotia,A.B., Lefkowitz,R.J., Young,J.A., Kumar,S. and
            Cook,D.I.
  TITLE     The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of
            epithelial Na+ channels
  JOURNAL   Proc Natl Acad Sci U S A 101 (32), 11886-11890 (2004)
   PUBMED   15284439
  REMARK    GeneRIF: Grk2 phosphorylates the C terminus of the Na-channel beta
            subunit and renders the channels insensitive to inhibition by
            Nedd4-2
REFERENCE   8  (bases 1 to 11179)
  AUTHORS   Miyazaki,K., Fujita,T., Ozaki,T., Kato,C., Kurose,Y., Sakamoto,M.,
            Kato,S., Goto,T., Itoyama,Y., Aoki,M. and Nakagawara,A.
  TITLE     NEDL1, a novel ubiquitin-protein isopeptide ligase for
            dishevelled-1, targets mutant superoxide dismutase-1
  JOURNAL   J Biol Chem 279 (12), 11327-11335 (2004)
   PUBMED   14684739
REFERENCE   9  (bases 1 to 11179)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   10 (bases 1 to 11179)
  AUTHORS   Miyazaki,K., Ozaki,T., Kato,C., Hanamoto,T., Fujita,T., Irino,S.,
            Watanabe,K., Nakagawa,T. and Nakagawara,A.
  TITLE     A novel HECT-type E3 ubiquitin ligase, NEDL2, stabilizes p73 and
            enhances its transcriptional activity
  JOURNAL   Biochem Biophys Res Commun 308 (1), 106-113 (2003)
   PUBMED   12890487
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124175.2, AC136517.3 and AC150896.6.
            
            On Oct 31, 2008 this sequence version replaced NM_001001883.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB182244.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMN00849374,
                                    SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-59                AC124175.2         170187-170245
            60-386              AC136517.3         71727-72053         c
            387-494             AC150896.6         20604-20711
            495-589             AC150896.6         28725-28819
            590-665             AC150896.6         34392-34467
            666-835             AC150896.6         38237-38406
            836-975             AC150896.6         40416-40555
            976-1076            AC150896.6         44360-44460
            1077-2450           AC150896.6         44842-46215
            2451-2546           AC150896.6         47531-47626
            2547-2697           AC150896.6         56893-57043
            2698-2801           AC150896.6         57655-57758
            2802-2926           AC150896.6         58160-58284
            2927-3112           AC150896.6         67091-67276
            3113-3247           AC150896.6         80265-80399
            3248-3350           AC150896.6         83840-83942
            3351-3447           AC150896.6         86100-86196
            3448-3531           AC150896.6         102606-102689
            3532-3641           AC150896.6         106375-106484
            3642-3762           AC150896.6         118236-118356
            3763-3876           AC150896.6         119839-119952
            3877-4029           AC150896.6         130659-130811
            4030-4128           AC150896.6         132415-132513
            4129-4258           AC150896.6         138774-138903
            4259-4405           AC150896.6         139869-140015
            4406-4520           AC150896.6         140769-140883
            4521-4606           AC150896.6         143555-143640
            4607-4719           AC150896.6         144537-144649
            4720-11179          AC150896.6         158192-164651
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 26.99 cM"
     gene            1..11179
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /note="HECT, C2 and WW domain containing E3 ubiquitin
                     protein ligase 2"
                     /db_xref="GeneID:329152"
                     /db_xref="MGI:MGI:2685817"
     exon            1..59
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            60..386
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..4831
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NEDD4-related E3 ubiquitin ligase NEDL2; E3
                     ubiquitin-protein ligase HECW2; NEDD4-like E3
                     ubiquitin-protein ligase 2; HECT, C2 and WW
                     domain-containing protein 2; HECT-type E3 ubiquitin
                     transferase HECW2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HECW2 isoform 1"
                     /protein_id="NP_001001883.1"
                     /db_xref="CCDS:CCDS14956.1"
                     /db_xref="GeneID:329152"
                     /db_xref="MGI:MGI:2685817"
                     /translation="MASSAREHLLFVRRRNPQMRYTLSPENLQSLAAQNSMPENMALQ
                     RANSDTDLVTSESRSSLTASMYEYTLGQAQNLIIFWDIKEEVDPSDWIGLYHIDENSP
                     ANFWDSKNRGVTGTQKGQIVWRIEPGPYFMEPEIKICFKYYHGISGALRATTPCITVK
                     NPAVMMGAEGMEGGASGSLHSRKLVSFTLSDLRAVGLKKGMFFNPDPYLKMSIQPGKK
                     SSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIEIKDKFAKSRPIIKR
                     FLGKLTIPVQRLLERQAGDQMLSYNLGRRLPADHVSGYLQFKVEVTSSAHEDASPEAV
                     GTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSFRTSSTLE
                     IDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSF
                     PTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPE
                     DDNPVENEDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTE
                     LCSSQEVDQPTSGADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTES
                     VSEASTRPEGESDPEGADSSCNESVTTQLSSVETRCSSLESARFPETPAFSSQEEEDG
                     ACAAEPTSSGPAEGSQESVCTPSSLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRR
                     GSLEGAAAAAPAAAATDSQPQEDGDAGDAQGACEGATAQEEGATGGSQTNGHQPLRSL
                     PSVRQDVSRYQRVDEALPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRS
                     NSIQQMEQLNRRYQSIRRTMTNERPEENTSAIDGAGEEADFHQASADFRRENVLPHST
                     SRSRLTLLLQSPPVKFLISPEFFTVLHSNPSAYRMFTNNTCLKHMITKVRRDTHHFER
                     YQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFFVDHNSRTTTFIDPRLPLQSSR
                     PTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYN
                     DKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLS
                     LFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLR
                     NFYRKLETKGYGQGPGKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGL
                     DYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRIL
                     GLALIHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLT
                     FTVNEEVFGQITERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFY
                     EVVDARLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERF
                     NNEQRLRLLQFVTGTSSIPYEGFASLRGSNGPRRFCVEKWGKITALPRAHTCFNRLDL
                     PPYPSFSMLYEKLLTAVEETSTFGLE"
     exon            387..494
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            495..589
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            590..665
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            666..835
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            836..975
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1076
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..2450
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2546
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2697
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2801
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..2926
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            2927..3112
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3113..3247
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3248..3350
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3351..3447
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3531
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3532..3641
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3642..3762
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3763..3876
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            3877..4029
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4030..4128
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4129..4258
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4259..4405
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4406..4520
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4521..4606
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4607..4719
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     exon            4720..11179
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6854..6859
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /note="hexamer: AATACA"
     polyA_site      6881
                     /gene="Hecw2"
                     /gene_synonym="A730039N16Rik; D030049F17Rik; Gm971;
                     mKIAA1301; Nedl2"
                     /note="major polyA site"
ORIGIN      
        1 agatgctgcc ccggcgtcta gcggacaagg cggcggcggc ggcggcggcg cggctggagc
       61 tttgtaggaa aacaacctca gagaggcaga caagatggct agctcagccc gggagcattt
      121 gctttttgtg cggcgtcgaa atccacagat gcggtacaca ctgagcccag agaacctcca
      181 gagcctggca gcccagaact caatgcccga gaacatggcc ctacagcggg ccaacagtga
      241 cacggatctg gtgacttctg agagccgctc cagcctgact gccagcatgt acgaatacac
      301 gctggggcaa gcccagaacc tcattatctt ctgggacatt aaggaagagg ttgaccccag
      361 cgattggata ggactctatc acatagatga gaattctcca gccaacttct gggactctaa
      421 aaacaggggt gtgactggaa cccaaaaagg acaaattgta tggcgaattg agcctgggcc
      481 ctacttcatg gaaccggaga tcaaaatctg tttcaaatac taccatggta ttagtggagc
      541 cctgcgagcc acaaccccct gtattaccgt gaagaaccca gctgtgatga tgggggccga
      601 aggcatggaa ggaggtgctt caggaagcct gcactctagg aaactcgtca gcttcacatt
      661 gtcagatctc agggcagttg ggctcaagaa agggatgttc ttcaatcctg acccttatct
      721 taagatgtcc attcagccag ggaagaagag cagttttcct acctgcgccc accatgggca
      781 ggagagaagg tccactatca tcagcaacac caccaatccg atctggcaca gagagaaata
      841 ctcctttttt gcacttttga ctgatgtctt agaaattgaa atcaaagaca aatttgccaa
      901 gagccggccc atcatcaaac gcttcctggg gaaactaacc attccagtgc agaggctgct
      961 ggagcggcag gctggggacc aaatgctcag ctacaacctt ggcagacggc tcccagctga
     1021 ccacgtgagt gggtacctcc agtttaaagt ggaagttaca tcttctgctc acgaagatgc
     1081 ttctccagag gctgtcggca ccatcctcgg agtccacact gtgaatggag acctgggcag
     1141 cccttccgat gaggaggata tgccaggcag ccatcatgac agtactatct gtgctaatgg
     1201 gccagtgtcc gaggacagtg ttgctgatgg cacccctaag cattctttca ggactagctc
     1261 taccctggag atcgacacgg aggacttgat ctctacttct tctagaaatt caccccccag
     1321 gggccgccag gattccctca atgattactt agatgctatt gaacacaatg gccccgccag
     1381 gccaggggca gcctcctcct ctgagcggtc catgggagcc tctcctaaac tgaggagtag
     1441 ttttcccact gacacaagac tcaatgcgat gcttcatatt gactcagacg aagaagacca
     1501 tgagtttcag caagacttgg gctacccatc ttctttggag gaggagggag gcttgatcat
     1561 gtgcagcagg gcgtcgagaa ttgatgatgg gagcctgaca tctcagacca aaccagagga
     1621 tgacaaccct gtggagaatg aggatgcctc tatacatgaa actgcatcct tagaagagag
     1681 gctggagaat cttccagagg ttgcagatgg ctccttgcca tcaagtacag cccctgatga
     1741 aaatgaagcc aacctggagc ctcaacccag tgcagaccag ggtagcactg aattgtgtag
     1801 ctctcaagaa gtagatcagc ccacaagtgg tgcagatgca ggagcttctg acacatctgg
     1861 agggagccgc agagctgcca gtgagaccga gtccctggac cagggatctg aaccttccca
     1921 ggtgtcctct gagacagaac ccagtgaccc tgcaaggaca gagagtgtga gtgaggccag
     1981 taccagacct gagggggaga gtgacccgga aggcgcagac agctcatgca atgagagcgt
     2041 gactacacag ctgtcctccg tggagacacg gtgctcatct ctagagagtg cgcgatttcc
     2101 agaaaccccc gccttttctt ctcaggagga agaagatggg gcctgtgcag ctgagcccac
     2161 cagcagtggc cccgcagagg ggtcgcagga gtccgtctgc actcctagtt ctttacctgc
     2221 tgtgcaggtg cccagcaggg aagaggaagg gtcagcggct gaagctgctg ctttatctga
     2281 gcagggggag ctaggggaag tctggcagag gagggggagt cttgagggag cagccgctgc
     2341 tgctcctgct gctgctgcca ctgatagcca gccccaggaa gatggggatg ctggggatgc
     2401 ccaaggtgcc tgcgaagggg ccactgccca ggaggagggc gccactggag gttctcaaac
     2461 caatggccac cagcccttga ggtcactacc ttccgtgcgc caggacgtca gccggtacca
     2521 gagggtggat gaggctctcc caccaaactg ggaggcacgg attgacagcc acggcaggat
     2581 cttctatgtg gaccatgtaa acagaaccac aacgtggcag cggcccacag ctccccctgc
     2641 cccacaggtc ctgcagcgat ctaactccat acagcaaatg gagcagctga accgacgtta
     2701 tcagagcatc cgcagaacca tgactaatga gaggcctgag gaaaatacca gtgctatcga
     2761 tggggcaggg gaggaagccg actttcacca agccagcgca gacttccgtc gggagaacgt
     2821 cctgcctcac tctacctcca gatccaggct cacgctgctg ttacagtcac cccccgtgaa
     2881 gttcctcatc agcccagagt tcttcaccgt gctgcattct aaccctagtg cctaccgcat
     2941 gtttacaaac aacacgtgtt tgaagcacat gatcaccaaa gtccggcggg acacccacca
     3001 ctttgagcgc tatcagcata acagggacct cgtgggattc ctcaacatgt ttgctaacaa
     3061 acagctggaa ctaccaaggg gctgggagat gaaacatgat catcagggca aggcgttttt
     3121 tgtcgaccac aactcccgga ccaccacgtt cattgatcct cggctcccac ttcagagcag
     3181 taggcccaca agtgcattgg ttcatcgaca acacctgacg aggcaacgca gccacagtgc
     3241 gggtgaggta ggagaagact ctcggcacgc aggaccacca gttcttccca ggccatccag
     3301 tacattcaat acggtcagta ggccgcagta ccaggacatg gttccagtgg cctacaatga
     3361 caaaattgtt gcgtttttgc gtcaacccaa catccttgaa attctgcaag agcgtcaacc
     3421 tgatcttgcc agaaaccact cactcaggga gaagatccaa tttatccgaa cggaagggac
     3481 ccctggattg gtgcggctct caagcgatgc agaccttgtc atgttactga gcttattcga
     3541 ggaagagata atgtcatatg tgcctcctca tgccttactc caccccagct actgtcagtc
     3601 cccgcgtggc tcccccgtat catctcccca gaactcacca ggtactcagc gtgccaatgc
     3661 ccgggctcca gccccttaca agagagattt tgaagccaaa ctaaggaact tttacaggaa
     3721 gttagaaact aaaggatacg gacaaggccc aggaaaatta aagctaatta tccgaagaga
     3781 tcacttgctg gaagatgctt tcaatcagat catgggctac tccagaaagg acctgcagag
     3841 gaataaactg tacgtcacct ttgtcgggga ggaagggttg gattacagtg ggccatccag
     3901 agagttcttc ttcctggtat cccgagagct ctttaacccc tattacggct tatttgaata
     3961 ttcagccaat gacacatata cagtgcaaat aagtcccatg tctgcctttg tagacaatca
     4021 tcatgaatgg ttccggttca gcgggaggat cctcggcctt gcgctcatac accagtactt
     4081 gctggacgcc ttcttcacgc gacccttcta taaggctctt ctcagaatcc tgtgtgacct
     4141 gagtgatctg gaataccttg atgaggagtt ccaccagagc cttcagtgga tgaaagacaa
     4201 tgacatccat gacatcctgg acctcacgtt cactgtgaat gaagaagttt ttgggcagat
     4261 tactgaacga gagttaaaac cagggggagc caatattcca gtcacagaga agaacaagaa
     4321 ggagtacatc gagagaatgg tgaagtggag gatcgagagg ggtgtggtgc agcagacaga
     4381 gagcctagtt cgtggtttct acgaggtagt agatgccagg ctagtgtctg tctttgatgc
     4441 gagagagctg gaactggtca ttgccggcac agctgagata gatctcaatg actggagaaa
     4501 caacactgaa tacagaggcg ggtaccatga caatcacatt gtaattcggt ggttctgggc
     4561 tgcagtggaa agattcaaca atgaacaacg actaaggttg ttacagtttg ttacaggcac
     4621 atccagcatt ccctatgaag gattcgcttc tctccgaggg agtaatggtc caagaagatt
     4681 ctgtgtggaa aaatggggaa aaatcaccgc tcttcccaga gcccacactt gttttaaccg
     4741 cctggatctg cctccatacc cttccttttc catgctttat gaaaaactct tgacagcagt
     4801 tgaagagacc agtacctttg gacttgagtg acctggaagc atgatgctca gctcttcatg
     4861 gccaggcggt tctagaagct ggcctcccga aaagtggaca cagcattggt agcctcaagc
     4921 cttggcttcc attataacaa actgagtgct gctttccggg aacacatgct cctgtgtttg
     4981 gggactggct gtgagttctt ttggaaggat tggggtgagc ttggtgccca tggaacaacc
     5041 caacagtctt tcagtcaaca gttcttgact gccaaacctt tcccatttgg tcccatgttc
     5101 caagataaaa gaagaacctg cacatgataa actccatggt gacttttagg gactcaggag
     5161 aatcttgaaa ctctcccata cataacactt caagccaaac tggcagcact gacccaatct
     5221 ctaaattagc gcaagtgaaa tgaaatcatt aaaaaaatat atatttcctg aaagtacatt
     5281 tgtttaagcc ctaagttata gcagaatatt tatttcttgc ttatgagtgc ctgcatggtg
     5341 tgcactatag gtttcaacct tcacaggaca agtgtgaaaa aggaaaccaa gtcattatgc
     5401 gataacttta aagataaggt ggtctgtgac aatgtatatg caagtggtat gtgtgtaatt
     5461 atggctgaag acaaacttac tcaatgaatc actaatgacc gattttatgc tttataatgc
     5521 atgaaaacaa ttttaaaata tctagcaatt aatcacagca tatcgggaaa atgtacacag
     5581 tgaattctgt ttatttttta taggttcatc atatttctgt tctttaagat gtatataagg
     5641 acctacctac catattgtat gtaccacctc tccatcttca tgttccatgc ttactcgggc
     5701 atcttgttag tatgtaccct tttaagcacg ctcaagggaa ctaaagggcc ttttattctt
     5761 ataaaagttt aaaaaaatcc ccaaaatatt ttgcactgaa agtaccaaag gcgaagggac
     5821 actacaatat gactaacagc agctccatca gttgacgact acaatagaag tagcttttaa
     5881 atcagacttc cttcacagtt gccatgtcaa ccactgcaag gactgtgcat ctaagtaata
     5941 attttgtaag actcactcta tgtgatagta tgatatgcat ttatttaaaa tgcattagac
     6001 tctcttccat ccatcaacta ctttacagga tggcatttat tactgatatt tcgtatttcc
     6061 cccactgctt cttatttgta cagcatcatt aaacactgag cttggttagg gagccatcag
     6121 caatgccgga gagatcagct ctctttcata gtaagagttc cattttctca catcagtgaa
     6181 ggtaccatac attgaaactc tcagtactat atttctaagc ctgcattttc actgatgtat
     6241 aattttctta ttaatatcaa gaaaaaattt tgtatggtct ctcagaaact gcatgtcatc
     6301 actgacgggt ctccttaatc tgatgaaaag gatttccttc atttcgattg ctttggggac
     6361 tgttccgtgt tgttgtttgt cccttgactt gtgaccacaa ctaatcagac acatgtacag
     6421 tgaagtggaa ttgaggtggg taagaccatt agcttaacat ttaatttgcc agcctgaggg
     6481 gtttacaggc attacctgaa cactgctttg agaatttctt tataggtatc atgggcttcc
     6541 tgttgtggga tgtgggtaac tttacatttt atacgggtta catacatcgt cttcataggt
     6601 gttttgtaag aacagttaca cgcctgtttg tgaagctgtg tttactgttg ggaccctcaa
     6661 gaacaatgac actcatgttc tgctattgtg ttaaagataa gtcctggata tgggaaaatg
     6721 ttctaggaga aaacaaatta cagaaacctg gaaaagatat atggaatatt tatttgtata
     6781 tcataattgg gccagaaaaa aagaatgact aaaatttttg actggaatta ttagtttgta
     6841 cttatttgtc atgaatacag tatatattta atttttgcaa actgctttta ctcgtttgta
     6901 ttttttcatc ataaggaaaa cagagcatgc taatacattt ttaaaatcac atttcgcttc
     6961 gtcagagcat tccctaagag ttgtcacagg ggcctgttaa gagagagctg cctttctttc
     7021 aaaaatacat ttataggcca cattagtgct cctttggttt gagacacttt gccaccatat
     7081 gccatattac taggcagaag agatttcaaa taaaatatac ctctttaact atttctaata
     7141 aacgttgttt gctgttgatg ctaaggcagc caacttctca aataacagtt ttaaatgtat
     7201 ccagacactc ttaaagtggt aggacttgtc ctagttggca ctgttctcag aattgggaat
     7261 tatcaaattg tagctagcat attagaagac tgtgttaacc cattattctt cataggcaaa
     7321 acaaaaacaa aaaaaagaca ataatatctc tataaaagtt aaataacaca tccacagaat
     7381 aaattagctg ttattgtacc ctacaattag aaatattcat aaatatgctt ttctggactg
     7441 tgaaccttga taacttggct ctttggtttt gttttaagag atttatacct gatgctacta
     7501 gcaatataaa agacagtctc gatacaccat gtatgtatca tttttcagag tacaggcaca
     7561 ctgttggagg cccctccatg ccatttgatg atggagctac aagattctga atctggcctt
     7621 cagaaaaatg cacatagtct actagagggg acatcaagat tccaaaagca ttttaaataa
     7681 aaggtctaca gttaatgaag agagaatagc ctattcaccc caaaggcttg gttacattct
     7741 catgtatgtg aaactgtata gctgaaagac atcatcacat atgtgctctt tctgctgagt
     7801 gtcatagaat atcaatgctg ataagtctcg agatccaaaa tgcattcata caatatgtgt
     7861 ttaaagtatg tgtatgagga ctgagctatg aaggagcaac acaaggatct tcaaactatc
     7921 aaagatgcca aaatgaaatt agagacttcc ttcagcctct gtactataac ttacattcta
     7981 tttttagtgt aaaatatcac agttcctgag cccaagccac atatggaatc acatttttct
     8041 tgtgcacaaa atgaatggaa gtcacagttt tctagtgtat gttgtattta taatttaatg
     8101 gccattctgt actaagtaaa cataaaagcc actattgaac tagaaaggga atgcagattg
     8161 caaaatggaa gtgtggcact tccagacatt cttattaaat taagctctag tcttaaaaac
     8221 aaaaggatga aggctgatct attcaacact acctcgatgg caatggcatg caaaggtagg
     8281 gactgtgagg actgtgtgct cagcgctgac gagtttccca gggcttgagg ggcattctct
     8341 attgctcctc agtgctcagt tggggagaag aatcaagtct tccctgggct caggctgatt
     8401 tcatgggtag ctggtaaaat gatttgggct ttgttgtcaa gatcctgttt agtcttaaga
     8461 aatataaaga ctaatatttg tatcaatata ctaaactttc caatgctttg ctttcccaaa
     8521 gtcttacttt tgtagacacc cttagaatgt atgtgacact cctgccgagg gcacggagaa
     8581 aggagtgcat tcatgcattc atggcaaagg cgtctcgaag gtgcattgtt tccatttact
     8641 gcctatggga ggaatttaag acaaatctga ttgtgattgg tggaattgca cagcacagga
     8701 atttggccct cttggggatt acgtagaagc aagagtatga aaaagacacc aaccagagac
     8761 ctggctttca ttattaattt tttatatcct tgagtcttca gaggcctggc agctgaaccc
     8821 cagtgatgtg gagggaccat agcagcttct gtttttcttt ctgtcttcct gatatttgtt
     8881 caaatgacct ttttaatcct tcttgcacac agctacttct aacaaggcat acatcttata
     8941 atgaagaatc tagaatttta ttcaatttaa ctttatttag gaaatcccag gagcaggaaa
     9001 tgaggccatt ttatcattaa aagtcatcac taaccccaca taagaagcaa gatgcaattg
     9061 aagaaactgg cccaacctcc cagcagttga gactcaggcc agctgttgat gagtggtatc
     9121 ctgaatcagc caccctactt ccacatgact cgacttgata ttaatgggaa tgccccttgg
     9181 aaacatattc cagttgtcag actttatttg ctttatagtt ctgttgcatt tccagacagt
     9241 ttgtctagca cagaagcaaa ggaatggcag agacattaca gctttctgag ctgtcactta
     9301 aggtcagtca ctacccttaa aggggtaatt agaaatcaaa agtggaaaag tagatgcctg
     9361 gcagttgtac caccctggta atgtgagccc aactactatc aagattcagg agcaaaaact
     9421 acattctaag gagctggcat tatgggtaga atgaatttgt ttggccctat cagtgcaatc
     9481 tgacttagga gtgaaggcac gtttacaatc acagaatagc taagggaaca ataaaacaat
     9541 ggtacaaaca tcatcagact tagacttcac tagtgggggt aggagcccgt tccattttgg
     9601 tttctgagtt ttgagaacaa tgcattcatg tttagtgaag gcattgattg gccatctacc
     9661 tggtgggaaa tacccttgtt gtatgacttc agtaggttag tatttcctgt actacaaaat
     9721 gtcatttagt cccaaagtat taaggctaac catagctcaa atcaggaagc tcaacaattt
     9781 acattaaatt attatagtta aaaaaaaagc ttgaaagtta tttgttacca ctatcagaca
     9841 ctatttgtga aataaaaaat gatcttattt catcatgctt aatttgtttt tatagttgac
     9901 attggcatgg cttttgagac cttcttatac tatctaatgc tgatttaaac attctctatt
     9961 aatctggaaa gcaaaaattc taagtgtgag gttactacat tgcaaaagga ggtctagcaa
    10021 gtattttata ttgaagaagt taatcatgac agcagacaca tttgcttaga ttttgatctt
    10081 caaggtactt tcttctcatt taatgattaa acagcttaac agaacaggag ggctctaact
    10141 gtatatgatt tttatgtcta gtgaccttgg gcaggtgata tcttcatatc caatcttctt
    10201 ttaagatatt gcaaccctga ggggtattgg taggtatgtt gtatgattca aactgaatca
    10261 gctattactg gtgtaagaga tcatcagtaa attccggtaa ttatcacctt ctgtaaaagg
    10321 gatgggtaat gttcaagaaa caccttggaa gggtgaacca gggaatatac agaacaaaat
    10381 gttctttagg accctaatgc cctaaaaaga tgttaactac agcttatttt tctttatgtg
    10441 atatcataag aaagatacag gggctgttag cacattagaa agtgcgtaag attatgtttt
    10501 tataataggg atgcaggcat tccaaaaaac tgaataaaaa tacatgcatc tctcttgcct
    10561 cttactgaag tattaatcta attttataat aatgcttact ttttaatatt aaggctatgt
    10621 aaaaaaaaac attttatttc taagatttca gtagagtaca ggtggtgcat tttttaagcc
    10681 tttccacttc tcttcaccag ccccgcccac tcattacatc cactatacca tagatcacag
    10741 ttaacaggta cacttctgat tttgttgagt tgattcaagc tttgtattta ttcagtgtaa
    10801 cttttagtag ataataataa tgggaggtat ttgctattag aattaagatt atacattcct
    10861 cattttaccg tacatttaca ttttttaagg cacataatat atgttgttga taggcatcct
    10921 agaagtagtt catgagtggt gttgcacaac acttaggact ggattatgga ccagttgttc
    10981 attttctagg aagtctgtat tcttattctc acctgagcct ccgcctcttg tgttgtattt
    11041 tttatgatta tgaaatgctt tgacggaaag atattttaat gtgtacaatt tgttttattt
    11101 tcatactgat ctctattttt gtttaaaacc atgttacatc atgaaatcac ttaatcaaaa
    11161 ataaatcttt taaaaaatg
//
