LOCUS       NM_001081348            9460 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus HECT, C2 and WW domain containing E3 ubiquitin protein
            ligase 1 (Hecw1), mRNA.
ACCESSION   NM_001081348 XM_897863
VERSION     NM_001081348.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Hung,C.H., Lee,T.L., Huang,A.Y., Yang,K.C., Shyu,Y.C., Wen,S.C.,
            Lu,M.J., Yuan,S. and Shen,C.J.
  TITLE     A Positive Regulatory Feedback Loop between EKLF/KLF1 and TAL1/SCL
            Sustaining the Erythropoiesis
  JOURNAL   Int J Mol Sci 22 (15), 8024 (2021)
   PUBMED   34360789
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9460)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9460)
  AUTHORS   Jin,Z., Liu,H. and Xu,C.
  TITLE     Estrogen degrades Scribble in endometrial epithelial cells through
            E3 ubiquitin ligase HECW1 in the development of diffuse
            adenomyosisdagger
  JOURNAL   Biol Reprod 102 (2), 376-387 (2020)
   PUBMED   31616916
REFERENCE   5  (bases 1 to 9460)
  AUTHORS   Qiu,X., Wei,R., Li,Y., Zhu,Q., Xiong,C., Chen,Y., Zhang,Y., Lu,K.,
            He,F. and Zhang,L.
  TITLE     NEDL2 regulates enteric nervous system and kidney development in
            its Nedd8 ligase activity-dependent manner
  JOURNAL   Oncotarget 7 (21), 31440-31453 (2016)
   PUBMED   27119228
REFERENCE   6  (bases 1 to 9460)
  AUTHORS   Wei,R., Qiu,X., Wang,S., Li,Y., Wang,Y., Lu,K., Fu,Y., Xing,G.,
            He,F. and Zhang,L.
  TITLE     NEDL2 is an essential regulator of enteric neural development and
            GDNF/Ret signaling
  JOURNAL   Cell Signal 27 (3), 578-586 (2015)
   PUBMED   25555806
REFERENCE   7  (bases 1 to 9460)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 9460)
  AUTHORS   Okazaki,N., F-Kikuno,R., Ohara,R., Inamoto,S., Koseki,H.,
            Hiraoka,S., Saga,Y., Seino,S., Nishimura,M., Kaisho,T., Hoshino,K.,
            Kitamura,H., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: IV. The complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 11 (3), 205-218 (2004)
   PUBMED   15368895
REFERENCE   9  (bases 1 to 9460)
  AUTHORS   Miyazaki,K., Fujita,T., Ozaki,T., Kato,C., Kurose,Y., Sakamoto,M.,
            Kato,S., Goto,T., Itoyama,Y., Aoki,M. and Nakagawara,A.
  TITLE     NEDL1, a novel ubiquitin-protein isopeptide ligase for
            dishevelled-1, targets mutant superoxide dismutase-1
  JOURNAL   J Biol Chem 279 (12), 11327-11335 (2004)
   PUBMED   14684739
REFERENCE   10 (bases 1 to 9460)
  AUTHORS   Martin,N., Jaubert,J., Glaser,P., Szatanik,M. and Guenet,J.L.
  TITLE     Genetic and physical delineation of the region overlapping the
            progressive motor neuropathy (pmn) locus on mouse chromosome 13
  JOURNAL   Genomics 75 (1-3), 9-16 (2001)
   PUBMED   11472062
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB606770.2, AK047678.1, AB083710.1, AK172932.1 and CT025604.10.
            
            On Dec 20, 2007 this sequence version replaced NM_001081348.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219382.74735.1,
                                           SRR10662772.3522561.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849380 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                BB606770.2         1-65
            66-935              AK047678.1         1-870
            936-2900            AB083710.1         439-2403
            2901-6451           AK172932.1         1186-4736
            6452-9460           CT025604.10        183042-186050       c
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 5.29 cM"
     gene            1..9460
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /note="HECT, C2 and WW domain containing E3 ubiquitin
                     protein ligase 1"
                     /db_xref="GeneID:94253"
                     /db_xref="MGI:MGI:2444115"
     exon            1..403
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            404..461
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     CDS             435..5249
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /EC_number="2.3.2.26"
                     /note="HECT type E3 ubiquitin ligase; mNEDL1; NEDD4-like
                     E3 ubiquitin-protein ligase 1; HECT, C2 and WW
                     domain-containing protein 1; HECT-type E3 ubiquitin
                     transferase HECW1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase HECW1"
                     /protein_id="NP_001074817.3"
                     /db_xref="CCDS:CCDS49207.1"
                     /db_xref="GeneID:94253"
                     /db_xref="MGI:MGI:2444115"
                     /translation="MLLHLCSVKNLYQNRFLGLAAMASPSRNSQSRRRCKEPLRYSYN
                     PDQFHNIDIRNGAHDAITIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDI
                     KEEVDAGDWIGMYLIGEVSSENFLDYKNRGVNGSHRGQIIWKIDASSYFVESETKICF
                     KYYHGVSGALRATTPSVTVKNSAAPIFKGIGSEETAQSQGSRRLISFSLSDFQAMGLK
                     KGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPT
                     DVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSG
                     QLQFRFEITSSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDA
                     KPESPSEGNGVNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPGELQDPEQHDT
                     QPTLSAEEVAEGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEE
                     EEEDVSTLEQGEPGLELRVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHT
                     LLHSMPSAQRGSTTEEEDGLEEESTLKESSEKDGLSEVDTIAADPQSMEDGESDGATL
                     CMAPSDCSGGHFSSLSKGIGAGQDGEAHPSTGSESDSSPQQGADHSCEGCDASCCSPS
                     CYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVDSAKISESTVFSSQEDEEEEN
                     SAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLESPMAGPSNRREGE
                     CPILHNSQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHINRTTTWQR
                     PSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGGGGG
                     GGSDSEAESSQSSLDLRREGSLSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAY
                     RVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQG
                     KSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASL
                     LARPGHSLIAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREK
                     IHYIRTEGNHGLDKLSCDADLVILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSP
                     QNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTF
                     NQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDT
                     YTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLKLPCDLSDL
                     EYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKE
                     YIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWR
                     NNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGL
                     RRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE"
     exon            462..786
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            787..894
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            895..989
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            990..1065
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1235
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1236..1378
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1479
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            1480..2823
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2824..2925
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..3076
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3077..3219
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3220..3341
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3342..3527
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3662
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3663..3768
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3769..3865
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3866..3949
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            3950..4059
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4060..4180
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4181..4294
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4295..4447
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4448..4546
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4547..4676
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4677..4823
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4824..4938
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            4939..5137
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     exon            5138..9460
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9438..9443
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /note="hexamer: AATAAA"
     polyA_site      9460
                     /gene="Hecw1"
                     /gene_synonym="9330116H24Rik; E130207I19Rik; Nedl1"
                     /note="major polyA site"
ORIGIN      
        1 agagagcaag cctggctcct ggttggttct caagtctggt tcccacagag gagcaggcag
       61 gaagggaggg gatgcgggag gaaggataaa gcaataaagt gtgctgcgtt gccacgcact
      121 gggagtcctt gtttgagccg caacaccacg agcctctgac ccagaggacc ccctcctggc
      181 tcgcacaggt tcaccagtcg agagctgctc cagccgtgac ggtggctctg acaatagttg
      241 gctcaggata cgaagcagcc agagcaaggc aaaagacgct gatcgcccag gactagcaaa
      301 ccaggtcctc aatgcctggc acctatgcaa gccacgtgcg acccccggtt ctaacctgcg
      361 tgcggaccgg agcatgatct gtgcccagtt ggaggctgcc aaggaattga tgcgcataca
      421 cgtggtggtt cattatgctg ctgcacctgt gtagtgtgaa gaatctgtac cagaacaggt
      481 ttttaggcct ggctgccatg gcgtctccat ctagaaactc ccagagccga cgccggtgca
      541 aagaacccct ccggtacagc tataacccag accaattcca caacatagac atcagaaatg
      601 gtgcccatga tgctatcacc atccctcgct ccaccagtga cactgacctg gtcacttcag
      661 acagccgctc tacactcatg gtcagcagtt cctactattc gatagggcac tcccaggatt
      721 tggtgatcca ctgggacatc aaggaagaag tggatgctgg agactggatt ggcatgtacc
      781 ttattggtga agtctcatct gaaaactttc tggattataa gaaccgtgga gtcaatggtt
      841 ctcacagagg ccagatcatc tggaagattg atgccagctc ttactttgtg gaatctgaaa
      901 ctaagatctg cttcaaatac taccacggag taagtggggc cctgcgagct acaaccccca
      961 gtgtcactgt caagaactca gcagccccta tttttaaagg tattggctct gaagagactg
     1021 cacaaagtca aggaagccgg aggctgatca gcttctccct ctcagatttc caggccatgg
     1081 gattgaagaa agggatgttt ttcaaccccg acccttatct gaagatttcc attcaacctg
     1141 ggaaacatag catcttccca gccctccctc atcatggaca ggaaaggaga tccacgatca
     1201 taggcaacac tgtaaacccc atctggcagg cagagcattt cagtttcgtg tctctgccaa
     1261 ccgatgtgct ggaaattgaa gtgaaggaca agtttgccaa gagtcgtccc atcatcaagc
     1321 gtttcttggg aaagctgtca atgcctgttc agaggctgct ggagagacac gccatagggg
     1381 atagggtggt cagctacaca cttggccgcc ggcttccaac tgaccatgtg agtgggcagc
     1441 tgcaattccg atttgagatc acatcctcca tccatgcaga tgatgaagag atctctctga
     1501 gtgctgagcc tgagtcgtca gcagaaacgc aggacagcat catgaatagt atggtgggaa
     1561 acagcaatgg agagccttct ggtgatgcta cagaattctg caaggatgcg aagccagagt
     1621 caccaagtga agggaatgga gtaaacagct cagaaaacca aaaccaggag catgccgggc
     1681 cagttgagga agcggcagga gctatggagg ccagagatgg tagcaatgtc tctgaagcac
     1741 ccgaagagcc cggagagctt caggacccag agcaacatga cacccagccc accctgagtg
     1801 cagaagaagt ggccgaggga cttcccctgg atgaggattc gccatcctcg ctgttgccag
     1861 aagagaatac agctctaggc agcaaggtag aagaggaaac agtaccagaa aatggagcca
     1921 gagaggaaga aatgcagaag gggaaagatg aagaagagga agaagaggac gtttctaccc
     1981 tggagcaagg agagcctgga ctggagctga gggtctccgt gagaaaaaaa agcagaccat
     2041 gttccctacc tgtatcagag cttgagacag tgattgcatc tgcctgtgga gatgctgaga
     2101 ccccgagaac ccattatatc cgcatccaca ccctgctgca cagcatgccc tcggcccaga
     2161 gaggaagcac cacggaggag gaagatggct tggaggagga atccaccctc aaggagtcct
     2221 ctgaaaagga tgggctcagc gaggtggaca caatagctgc tgacccacag tccatggaag
     2281 atggcgagag cgatggggct actctgtgca tggcaccctc tgactgctct gggggacatt
     2341 tctccagttt atccaaaggc attggtgctg gccaagatgg cgaagctcac cccagcactg
     2401 ggagtgagag tgactccagc ccccagcagg gagcagacca cagttgcgag ggctgtgatg
     2461 cctcatgctg cagcccgtct tgctatagca catcttgcta cagcagctct tgttacagca
     2521 gctcctgtta cagcagctcc tgctacaatg gtaacaatcg gttcgccagc cacacacgct
     2581 tctcctctgt ggacagcgcc aagatctctg agagcacagt cttttcttca caagaggatg
     2641 aggaagagga aaacagtgca ttcgagtcgg tacccgactc ggtgcaaagc ccagagctag
     2701 atcccgagtc tacaaatggg gctggccctt ggcaggatga actggctgcc cctggtggga
     2761 atgcagcaag aagtactgaa ggtctggagt cccccatggc aggtcctagc aataggagag
     2821 aaggtgaatg tcctatactc cataattccc agccaataag ccaacttcct tccttgaggc
     2881 ctgaacatca tcactaccca acaatcgatg agcctcttcc accaaactgg gaggctcgaa
     2941 ttgacagcca tggacgggtc ttttatgtgg accatataaa tcgcacaacc acctggcagc
     3001 gaccatcgat ggcccctacc cccgatggaa tgatcagatc cgggtctgtc caccagatgg
     3061 agcagcttaa cagaaggtat caaaacatcc agaggaccat ggcaacagag agagctgaag
     3121 aagattctgg caatcaaaac tctgagcaaa taccagacgg aggaggagga ggtggtggtg
     3181 ggagtgactc ggaagcagaa tcatcccaat caagcttaga tctgaggaga gaagggtcac
     3241 tttctcctgt caactcacag aaggtcacct tgctgctgca gtctccagcg gtcaagttca
     3301 tcaccaatcc tgagttcttc actgtgttgc atgccaatta tagtgcctac agggttttca
     3361 caagcagcac ttgcctgaag catatgattt taaaagtccg aagagatgct cgaaattttg
     3421 agcgctatca gcacaaccgg gacctggtga atttcatcaa catgtttgca gatactcggc
     3481 tggaactgcc ccggggttgg gagatcaaaa ctgatcatca gggaaagtct ttcttcgtgg
     3541 accacaacag tcgagctact actttcatag atccccgaat ccctcttcag aatggccgtc
     3601 ttcccaatca tctaacccac cgacagcacc tccagaggct ccggagttac agtgctgggg
     3661 aggcctcaga agtctctaga aacagaggag catctttgct ggccagacca ggacacagcc
     3721 taatagctgc tattcgaagc caacatcaac atgaatcctt gccactggca tacaatgaca
     3781 agattgtggc atttcttcgc cagccaaaca tttttgaaat gctacaagaa cgtcagccaa
     3841 gcttggcaag aaaccacaca ctcagggaga aaatccatta tattcggaca gagggcaatc
     3901 atgggcttga taaattgtcc tgtgatgcag atctagtcat tttgctgagt ctctttgaag
     3961 aagagatcat gtcctatgtt cccctgcaat ctgccttcca ccctggatat agcttctctc
     4021 cccgctgttc accctgttcc tcacctcaga actccccagg tttacagaga gccagcgcaa
     4081 gagccccttc accataccga agggattttg aagctaagct ccgcaatttc taccgaaaac
     4141 tggaagccaa aggatttgga cagggtccgg gtaaaattaa gctcatcatc cgtcgagacc
     4201 acttgttgga ggggaccttc aatcaagtga tggcatactc aaggaaggag ctccagagaa
     4261 acaagctcta catcaccttt gttggagagg aggggctgga ctacagtggc ccttctcgag
     4321 agttcttctt cctgttgtct caggagctct tcaaccctta ctatggactc tttgagtatt
     4381 ctgcaaatga cacatacaca gtacagatca gccccatgtc agcatttgta gaaaactatc
     4441 ttgaatggtt tagattcagt ggtcgtatcc tgggcctagc tctgatccat cagtacctcc
     4501 tggacgcttt cttcacaaga cccttctata agggactcct gaagctgcca tgtgatctga
     4561 gtgacctaga atatttggat gaggaattcc accagagttt acagtggatg aaggacaaca
     4621 acatcacaga cattctagat ctcaccttca ctgttaatga agaagttttt ggacaggtaa
     4681 cagaaagaga gttgaagtct ggaggcgcca acacgcaggt gactgagaaa aacaagaagg
     4741 aatacataga gaggatggtg aagtggcgtg tggagcgtgg tgtggtacag cagacggagg
     4801 cattgctcag gggtttctat gaggttgtag actcaaggct cgtctcagtg tttgatgcga
     4861 gggaactgga gctggtgatc gctggaactg ctgaaatcga cctgaatgac tggaggaata
     4921 acacagagta ccggggaggt tatcacgatg gccaccttgt gatccgctgg ttctgggctg
     4981 cagtggaacg cttcaataat gagcagagat tgcgattact tcagtttgtg acaggaacat
     5041 caagtgtgcc ctatgaaggc tttgcagctc tgcggggaag caatgggctt cgacgtttct
     5101 gcatagaaaa gtgggggaag attacatctc ttcccagggc acatacgtgc ttcaaccgat
     5161 tggatctccc accataccct tcatattcca tgttgtatga aaagctgtta acagcagtag
     5221 aagaaaccag cacctttgga cttgagtgaa gacgtggagc cttgcctggc attgtcctga
     5281 acagtgacac caccctctct gggatgactt actccctcct ttgactttgg tattccatga
     5341 tttttatttc aaagcaaatt agaattggca aaagctgtgc atgaagaatt gccttcttct
     5401 aagatctaat cttcaggctt atctcctctg tttccaatga actgctagcc tgtatgcaat
     5461 actacaaaac agctgtctca aggtctgtgt atacttccac atacctccat tcctaacaat
     5521 gaaatatgag tgcaagttat gctacacttg acgaaatggt aataaatgtt tacttccatt
     5581 tctatcattt aaagataatt tgagctgtaa gcattccatg cttttaaatg cccatgtctt
     5641 ccaaacagaa cctccatttt tcctaatttc tagcaactat ttccagacct gggagctgac
     5701 taattttttc ctttctatct ctacatttcc tattcattct atccaaatgg acagccaagg
     5761 ccacacagaa acgttttctt cattcaactc atgattcata gtggtatcag ccccatcagc
     5821 cagaactaac ctagacatat ggtgcaaata tgaaacttcc agcaattaac aacagcaaga
     5881 aggaaaaggt tatcagtaaa tccaagggtt atatggacta tgaaatcaac ttgagttcag
     5941 aaaagtgagg tgggggatct gccctggtga accctcacca cccaagaagc acaaacttgt
     6001 gtaccatgtt caaggagcaa atcctaattg ctgtagattg aaggatgagc ctttacctct
     6061 aattttgtca ccataaaaag tgataaggca aacaggaaaa aggacaggac atatgacatt
     6121 tattccaatc gatgaatttt gtttaatcta ttctcacgca cacatagtcc aaataaatct
     6181 catggctttt gttgcagcca agtttattta cgtgataggc caaacttttg cttcttttta
     6241 gctttgatac ttttctcatt cttttttttt tcctttcctt tccttttttt aaatcatggt
     6301 gttcaaattc tgtcacatct gatgttcaca gacaccacat acaaagctgc agagcacgat
     6361 ggtgcttgtt aaggatcacg ggcaatataa acttttcctt catccttagt gattcttcaa
     6421 aggcacgctg acttgtgtcc acaaaaaaaa aatccatttt tttaaagcat tcagcaaaat
     6481 atttaagaaa atgtgttttt agaaaaacaa tcacaggctc cacagacagc ctggcatctt
     6541 aagtacattt gagataaatt taaccataat ggtcatttgc tattttttgt aaccatgtta
     6601 tccatgttgt ctgaaatacg acataaatga aagtggtttt tcttcactgg gaaaaaaaaa
     6661 agctaaagag acccaactat ttaaatattc attgttgtag tcaacatatt aaaataaaag
     6721 aacaagtctg agaatgtaaa gctaacttag gatgaagcca tgcctcgtgg ctcagtacag
     6781 cagtgtgttt tgttcctgca cattaaaata ttttcttagt aaaaattatt cacaatgcca
     6841 agacactctg tgttcctgtg tttctttgtt aagcaagaag tatatatgat acaaacttag
     6901 ttttctattg ctatcaaaaa taagttttac taaaaaatgg gatgtacatt gtatgaaaca
     6961 cagcttgagg ggtaagattt ggcccatatt tcagaatctg aaaaataagt ccaatggcgc
     7021 tgttatttac taatgaactc ggaacaaaag cactgctaaa gtcattttca ctaaatcttt
     7081 aactcctaga atagcatatt tagaattcat cttatacata cataaaaacc tcacaaaaaa
     7141 attcaaccat tcaagttttt taccactgac ttgccataca ttgaaaagga gaatgttgtt
     7201 caaatgtagg aagctgctcc tgaagagctt tacaatttgc agaaatgaga caatctatta
     7261 aatagattaa tatatagtct accaacatac agaaggaaga aattttctta gaaatgacat
     7321 gtgaataaga aactcagata aatgcatttt taaattaatt tttagttata cctctaccct
     7381 tatgcaaggt tctacacaaa tcccaagagc aatagcattt ctaacatact cgttttaatt
     7441 atttgaagtt attgcgatgt atttcaatca tattcaccta acactcttct ccctaactcc
     7501 ttgttcattc actccccaat attccattcc ttctcagtgt catgtcattt tttaatgacc
     7561 cattgagtcc aatttgtgct gtttgtccat atcttaaatt agtatgaggt catcccctgg
     7621 aagatataaa tgtatcattt tgtgtgtgtg tgtgtgtgtg tacatacaca tatacatatt
     7681 tgattgtcat agccggccgc agtggtgcac ctttatggca ttaaaatgcc tttaatccca
     7741 gcacttggaa ggcagaggca ggcagcgctc tgtgagttca atgccaatct ggtcaacaga
     7801 gcaaattcca gatcatccag agttacctag taagactcta aaaaaaaaaa aaaaaaagac
     7861 aaaaggaaag aaagaaaact gactcttagc agctatcggc tgtcaatagc tcctcagata
     7921 gggctgggga cttgtgaacc cctctttata tactaatagt attctagaaa aattaaaaat
     7981 gtcatttata ctgacaccaa agttgctgag agaatgtaaa gcttgctttg cagagcccaa
     8041 cacgtgactt cagcctttca tggaagaagg gaaaacaaag gggagaggag ctcaaaagcc
     8101 agtcatggat agacacgtcc cacttcatcc atgagaacat tgaaattatg tctaattcat
     8161 tatatatgca tatggacata taactatata tgtttatatg tatatgtgta tacatttaca
     8221 tgtaaggaaa atgctgtaga aatagttttt tattgatttc aatggttgga gataaatcac
     8281 ctcaaatcat ataattcttt gggtaacaca gatttttttt ttgtgaccat atgttattct
     8341 ttgaattttt ttctccataa agttaattag aaatttatta cctgtaagtc ataacctccc
     8401 taaaacaaga tttttttata tctagtttta tgagtataat acaatacctt ttgggagaag
     8461 acaaaaatat ttaaaagatt taaaaaagta tgttataatt tgaaaaagac aataaggtgt
     8521 tataatttta tagataatac aaacatatta ttcaataaca tgaagatgac aatcttccca
     8581 atagaaactg agtcatcaag atcagaagta ctctagaata ctgagttctc tgtgacatcc
     8641 cgtgtatatg agttcgagaa caccctgatg gtttactacc atcattatta tagcacaaac
     8701 acttcaggaa aggtaatgaa aatattcaaa tgcttaatga gattttggca agctttcatt
     8761 atgaaagatt taaatgcttt tcctgttaat aatgaagcta tatcattcaa cagtaagaat
     8821 atttgtatgg caagccagtg agctgagttt cccacaaaat gtctaacaat attcagtttc
     8881 atcaatagtt catgtatcaa gccacatgtc tgaaatttgg gttcagaaaa tttcttaagt
     8941 tgccacaaat gttttcccag tcaagatgaa acactttcaa atgaaagtag ttagaacttt
     9001 acataacaaa atgtggtgct ttattattat tgttgtcgtc gttgttatga ttattattat
     9061 tattacaatt atgtctaaca gtgttcatca ctaaaactgc aatactgtat cataaatgtg
     9121 caggatgact attccagcca tcacaaggtc caaaagagaa agtgttattt tcctgttaat
     9181 gacatgtagt ccctttgttc tagtagctaa aaggtgccta gaggtagtat atagagtaaa
     9241 tattgttccg ttagtctatt tcctgcagct tccattatct aaggaaatgt ccataagatt
     9301 ttgtttacaa gtctttgttc tagtcaataa caggagacgt catattactg ggctgatttt
     9361 gcttacatct acaaaagaaa tgttatgtga tactcaggcc tcgctttctc ttacatcata
     9421 catcttcaag gaatgtcaat aaactcactt tggtatggca
//
