LOCUS       NM_172637               4771 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus HECT domain E3 ubiquitin protein ligase 2 (Hectd2),
            transcript variant 2, mRNA.
ACCESSION   NM_172637
VERSION     NM_172637.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   3  (bases 1 to 4771)
  AUTHORS   Coon,T.A., McKelvey,A.C., Lear,T., Rajbhandari,S., Dunn,S.R.,
            Connelly,W., Zhao,J.Y., Han,S., Liu,Y., Weathington,N.M.,
            McVerry,B.J., Zhang,Y. and Chen,B.B.
  TITLE     The proinflammatory role of HECTD2 in innate immunity and
            experimental lung injury
  JOURNAL   Sci Transl Med 7 (295), 295ra109 (2015)
   PUBMED   26157031
  REMARK    GeneRIF: studies describe an unreported innate immune pathway and
            suggest that mutation or antagonism of the E3 ligase HECTD2 results
            in reduced severity of lung inflammation by selectively modulating
            the abundance of the anti-inflammatory protein PIAS1
REFERENCE   4  (bases 1 to 4771)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 4771)
  AUTHORS   Lloyd,S.E., Maytham,E.G., Pota,H., Grizenkova,J., Molou,E.,
            Uphill,J., Hummerich,H., Whitfield,J., Alpers,M.P., Mead,S. and
            Collinge,J.
  TITLE     HECTD2 is associated with susceptibility to mouse and human prion
            disease
  JOURNAL   PLoS Genet 5 (2), e1000383 (2009)
   PUBMED   19214206
  REMARK    GeneRIF: Hectd2 is identified as a quantitative trait gene for
            prion disease incubation time in mice.
REFERENCE   6  (bases 1 to 4771)
  AUTHORS   Nishimura,M., Yokoi,N., Miki,T., Horikawa,Y., Yoshioka,H.,
            Takeda,J., Ohara,O. and Seino,S.
  TITLE     Construction of a multi-functional cDNA library specific for mouse
            pancreatic islets and its application to microarray
  JOURNAL   DNA Res 11 (5), 315-323 (2004)
   PUBMED   15747579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK142777.1, AK029549.1, CB526486.1 and BP754340.1.
            
            On Jul 31, 2009 this sequence version replaced NM_172637.2.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the middle portion of the coding region, compared to
            variant 1. This results in a shorter protein (isoform 2) compared
            to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029549.1, SRR11927937.5105803.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-141               AK142777.1         1-141
            142-1088            AK029549.1         2-948
            1089-1089           CB526486.1         542-542
            1090-2405           AK029549.1         950-2265
            2406-4758           AK142777.1         2233-4585
            4759-4771           BP754340.1         9-21                c
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 31.77 cM"
     gene            1..4771
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /note="HECT domain E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:226098"
                     /db_xref="MGI:MGI:2442663"
     exon            1..277
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            278..407
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            408..546
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            547..649
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            650..739
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            740..804
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            805..850
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            851..960
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1109
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1233
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1330
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1438
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1571
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1572..1660
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1661..1819
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1820..1894
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1895..1982
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2089
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2090..2205
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2206..2349
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..4761
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4738..4743
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      4755
                     /gene="Hectd2"
                     /gene_synonym="4921524L07; A630025O09Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcgcgggggc gggggcggcg cggcggcggc ggttggaagg agccctctcg cggccgcggc
       61 agcagcagca gcgcccgccc cggctgcagt ccggccgccg ctgccgccgc cgccaccgcc
      121 tcgcgcctca gccgcccggc cagacatgag tgaggcggct cgggatctct cgcccggagc
      181 gccgccggcg gtagccgcgg ccgccccgga ggagaggaag gggaaagaac cggagcgcga
      241 gaagctgccg cccatcgtga cggccggcgc agccgcgggc ttggacagag gatccaaggg
      301 ccagatttcc accttcagca gctttgtctc gactgtcacc cagaagaagg aagctgctga
      361 gaacagaagc tcgcctaccc accttgctct ccctaacatc aggaatgtga gagacctacc
      421 accaatttgt cttgatgtta gacaaaaaca gcgcatgtct gtggaagcgt taccatctga
      481 agtgaaggtc ccgccccttc cagaaccttc tcttcctagc cagcccaaaa ctgtaaaaga
      541 ctttgaggaa gatttagaaa aagctgaggc cacagggaat tggaagacag tacatgcttt
      601 ttatataaca gcatttgatt ctttcacaga actaaacact gcatttaaga aagatgccac
      661 ggcctcattt aatactattg aggactctgg gcttaatgcc aatttggtga atgctgtgtt
      721 tgatgcctta cttaatactc ctcaagatat ccagaagtca gtattgaagg gaatcattaa
      781 cagcttgtta caagaatgga aaggtccacg aacaaaagat gatcttagag catattttat
      841 actgttacag aatcctcaat ttaatatcac atctacatat gtcatctatg ctcatttgct
      901 acgacagata gctaccttag tggaagctga ccatcacttc ctagttcact ggcttaaaaa
      961 attatcccag aagaaattca agcaactggt agagagattg ctgcaatttg tttctttacg
     1021 gctgtttcca gcaaaacctg aagaattccc gcctctaacg aagtgtacct ggtggatccc
     1081 atcagccgct aaagttctgg ctttgcttaa cactgccaac aatttggttc accctcccct
     1141 cgttccttac actgatttct ataattctac cctggatcac attgatctca tggaagagta
     1201 tcacacgtgg cagagctttg ggaactctca caggttttcc ttctgtcagt acccattcgt
     1261 tatttctata gctgcaaaaa aaatcattat tcaaagagac tcagaacaac agatgataag
     1321 catcgcaagg caaagtctgg tggataaagt atctcgaaga caaagacctg atatgaatat
     1381 gttatttcta aatatgaaag taaggaggac acatctggtt agcgactcac ttgatgagtt
     1441 aacccggaag agagctgact tgaagaagaa gttgaaagtt acatttgtag gtgaagctgg
     1501 tttggatatg ggcggcttga ctaaagaatg gttccttctt ctaattcgcc agattttcca
     1561 tccagattat ggcatgttta cataccacaa ggattcacac tgccattggt ttagcagctt
     1621 taaatgtgat aactattctg aattccgatt ggttggaatt ctcatgggac tagctgttta
     1681 taacagcatc accttggata ttcgttttcc tccctgctgt tacaaaaaat tactgagccc
     1741 tcctgtcgta ccaagtgatc agagtacacc agtaggcatc tgcagtgtta ccattgacga
     1801 cttgtgccag gttatgcctg aattggccca tggactaaag gaacttttat catatgaagg
     1861 caacgttgaa gaagatttct attcaacatt tcaggttttt caagaagaat ttggagtaat
     1921 taaatcctat aacttaaaac cagggggtga taagattcca gttaccaatc agaatagaag
     1981 agagtatgta cagctttata ctgacttcct gttgaacaaa tctatctata agcaatttgc
     2041 tgcattttac tgtggatttc atagtgtgtg tgcttcaaat gccctaatgc tgcttcgtcc
     2101 agaagaagta gaaatcctgg tttgtggcag tcctgaactg gatatgcatg cactgcagag
     2161 gagcacacag tacgacggct atgcgaagac agacctgacc atacgatact tttgggatgt
     2221 tgtgcttgga tttccacttg aacttcagaa aaagctgtta cactttacta caggaagcga
     2281 cagagtacct gttggaggga tggctgattt gaattttaaa atttcaaaga atgaaacttc
     2341 tactaactgg ttacctgtgg cacacacttg tttcaatcaa ctctgccttc ccccatacaa
     2401 gagcaaaaaa gatctgaaac agaaactgat tattggaatt tcaaattcag aaggttttgg
     2461 acttgaataa cctagaagac ttgaaataca gtatctatat gtagcactca gctccctctt
     2521 actgtgcctt taacactttc atgcttctgt ttcaaaacac tttctcaaag ctcaccactt
     2581 ttaaaacaat gagggtcttt ttttatttac tccgatacaa aatatgtata gtgaaggcat
     2641 gatttaaaaa tatataaata tatatatata tcagaaattt attgaatgag agcctatggc
     2701 agaaacaggt ctgagtccgg cagtcttttt tatagtgttt tgttgttttt ccatatgtag
     2761 tttgtcacag gtgtccactg ggaaagacaa gtacagtaag aatgcatctc tgacatgatg
     2821 gagcccagtg gcagatgtgt gctggcagca cactgtagca aagcagagag ctacattatc
     2881 tttaccataa agcatgtagc catattttca tacagaggta acaacagtat cactgccaat
     2941 gcagtgtaca gggtcttttg atatattggt tttggggtcc tataagatct ttccacccgt
     3001 tgctgaaaag catgtaaata acactacaca gcagccgaag aatcatggga ttttgatagt
     3061 gccatttatt gctaaagatt tatttttaca aagtaaattt agggttttag atgtgtgtac
     3121 agcttttaca aatcaacaaa gatgattgta caagagtatt ttcagaccaa ttggattcaa
     3181 ggttatattc ttttaagtat tgttagtctg catgcccact gacagtaaat tggttacttg
     3241 agtattctgt aatgtttgta taatggttat ttcttcttaa aaatcaatat agtagagcaa
     3301 actagaccaa actagtcatt gattacttaa aataattaca agcacaaaag aagccctgct
     3361 attttatata ttttgatgta ctgcttatat ctacaatttt tgatcaccag ttatatgaaa
     3421 acactaattt ttggaaaatg taggatattt gacaaaagtg aaatacaatt aaaaacattt
     3481 atgtcaaaat gatgtagctt gatataaaaa agaagtttga cattttggct aaaatgtgtg
     3541 tttttcactg gattctaaat atagtaaatc caaaagtatc cttgttttct atttataata
     3601 aaatagcatg taaaaactgt atttttaagc cccagggatt tttgttttgt tttgttttgt
     3661 tttgttttaa tttatagtga agcagtttta ttagcatgtc acaaatattt tcatctcaag
     3721 aattattctt tgatagtttt tcttcagaaa actctgaagt gacatttgca ataagaatga
     3781 aaccagcatt tagtaccagc ttatggccca tgtgcaaatc atacttggcc cgaggctccc
     3841 ctgagctagg catttccttg tcgctgtgct ttctgcaccc aaccagcaga attcaagggt
     3901 gcagtaactc atcagcgtcc tatagttccg aagcccaccc acccgtgtta gaaaacccag
     3961 cttccttcct gtaacgcttg agactgcaaa tgactacttt cccatgtgag tttaccagaa
     4021 gggtagttgt tctttaaatc gtgggaagcc taagacagct aaaggtacag ttggtttggt
     4081 gtgtatattg tctttaatcc atatgcaaat aaaactgctt ctctcttgca ctgcgtagaa
     4141 atgtttaatg taggttccta ctaacaagca caaagaatta tgtataggaa accagattta
     4201 agtgttttca gtaaaatgta aacaaagttt ttatttgtta gggatgactc atggaaatag
     4261 tattggtttg gtctgcatgt ctaacgatgt gtgtgtattg gtagctatgt cacttttgca
     4321 agtacacagg caagccaaat tttaggtgac aaaagtccat aaataatggg aatttttgtt
     4381 gtcttttatt aagttgaagt tgataaccat taattgctaa accatatatt tatgctctgt
     4441 tttggtttac aatgtaataa tacctcattt taaaagtaaa aactactact gttacatttt
     4501 attttaatca gctagaaaat tcatgtagct ttttaaatat acaacctatt aatgatttct
     4561 tgagtttttc tatctgccat agtaaattaa agcattgcac agtatttatt agtatttata
     4621 aaatgtcttg tccttttttt aaaaacctat tatttatttt ttctgtataa gtttttagaa
     4681 gtccttcttt tgtataaatc tgtattgtgt tgagtttatt tgtgtacatt ttcaaatatt
     4741 aaaattatac aatcagtcag gaaaaaaaaa a
//
