LOCUS       NP_034540                130 aa            linear   ROD 27-APR-2025
DEFINITION  hypocretin neuropeptide precursor precursor [Mus musculus].
ACCESSION   NP_034540
VERSION     NP_034540.1
DBSOURCE    REFSEQ: accession NM_010410.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 130)
  AUTHORS   Chen,B., Jin,K., Dong,J., Cheng,S., Kong,L., Hu,S., Chen,Z. and
            Lu,J.
  TITLE     Hypocretin-1/Hypocretin Receptor 1 Regulates Neuroplasticity and
            Cognitive Function through Hippocampal Lactate Homeostasis in
            Depressed Model
  JOURNAL   Adv Sci (Weinh) 11 (38), e2405354 (2024)
   PUBMED   39119889
  REMARK    GeneRIF: Hypocretin-1/Hypocretin Receptor 1 Regulates
            Neuroplasticity and Cognitive Function through Hippocampal Lactate
            Homeostasis in Depressed Model.
REFERENCE   2  (residues 1 to 130)
  AUTHORS   Braga,A., Chiacchiaretta,M., Pellerin,L., Kong,D. and Haydon,P.G.
  TITLE     Astrocytic metabolic control of orexinergic activity in the lateral
            hypothalamus regulates sleep and wake architecture
  JOURNAL   Nat Commun 15 (1), 5979 (2024)
   PUBMED   39013907
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 130)
  AUTHORS   Yang,D., Wang,Y., Qi,T., Zhang,X., Shen,L., Ma,J., Pang,Z.,
            Lal,N.K., McClatchy,D.B., Seradj,S.H., Leung,V.H., Wang,K., Xie,Y.,
            Polli,F.S., Maximov,A., Gonzalez,O.C., de Lecea,L., Cline,H.T.,
            Augustine,V., Yates,J.R. 3rd and Ye,L.
  TITLE     Phosphorylation of pyruvate dehydrogenase inversely associates with
            neuronal activity
  JOURNAL   Neuron 112 (6), 959-971 (2024)
   PUBMED   38266644
REFERENCE   4  (residues 1 to 130)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (residues 1 to 130)
  AUTHORS   Liao,Y., Wen,R., Fu,S., Cheng,X., Ren,S., Lu,M., Qian,L., Luo,F.,
            Wang,Y., Xiao,Q., Wang,X., Ye,H., Zhang,X., Jiang,C., Li,X., Li,S.,
            Dang,R., Liu,Y., Kang,J., Yao,Z., Yan,J., Xiong,J., Wang,Y., Wu,S.,
            Chen,X., Li,Y., Xia,J., Hu,Z. and He,C.
  TITLE     Spatial memory requires hypocretins to elevate medial entorhinal
            gamma oscillations
  JOURNAL   Neuron 112 (1), 155-173 (2024)
   PUBMED   37944520
REFERENCE   6  (residues 1 to 130)
  AUTHORS   Chemelli,R.M., Willie,J.T., Sinton,C.M., Elmquist,J.K.,
            Scammell,T., Lee,C., Richardson,J.A., Williams,S.C., Xiong,Y.,
            Kisanuki,Y., Fitch,T.E., Nakazato,M., Hammer,R.E., Saper,C.B. and
            Yanagisawa,M.
  TITLE     Narcolepsy in orexin knockout mice: molecular genetics of sleep
            regulation
  JOURNAL   Cell 98 (4), 437-451 (1999)
   PUBMED   10481909
REFERENCE   7  (residues 1 to 130)
  AUTHORS   Siegel,J.M.
  TITLE     Narcolepsy: a key role for hypocretins (orexins)
  JOURNAL   Cell 98 (4), 409-412 (1999)
   PUBMED   10481905
  REMARK    Review article
REFERENCE   8  (residues 1 to 130)
  AUTHORS   Yamamoto,Y., Ueta,Y., Date,Y., Nakazato,M., Hara,Y., Serino,R.,
            Nomura,M., Shibuya,I., Matsukura,S. and Yamashita,H.
  TITLE     Down regulation of the prepro-orexin gene expression in genetically
            obese mice
  JOURNAL   Brain Res Mol Brain Res 65 (1), 14-22 (1999)
   PUBMED   10036303
REFERENCE   9  (residues 1 to 130)
  AUTHORS   Sakurai,T., Amemiya,A., Ishii,M., Matsuzaki,I., Chemelli,R.M.,
            Tanaka,H., Williams,S.C., Richardson,J.A., Kozlowski,G.P.,
            Wilson,S., Arch,J.R., Buckingham,R.E., Haynes,A.C., Carr,S.A.,
            Annan,R.S., McNulty,D.E., Liu,W.S., Terrett,J.A., Elshourbagy,N.A.,
            Bergsma,D.J. and Yanagisawa,M.
  TITLE     Orexins and orexin receptors: a family of hypothalamic
            neuropeptides and G protein-coupled receptors that regulate feeding
            behavior
  JOURNAL   Cell 92 (4), 573-585 (1998)
   PUBMED   9491897
REFERENCE   10 (residues 1 to 130)
  AUTHORS   de Lecea,L., Kilduff,T.S., Peyron,C., Gao,X., Foye,P.E.,
            Danielson,P.E., Fukuhara,C., Battenberg,E.L., Gautvik,V.T.,
            Bartlett,F.S. 2nd, Frankel,W.N., van den Pol,A.N., Bloom,F.E.,
            Gautvik,K.M. and Sutcliffe,J.G.
  TITLE     The hypocretins: hypothalamus-specific peptides with
            neuroexcitatory activity
  JOURNAL   Proc Natl Acad Sci U S A 95 (1), 322-327 (1998)
   PUBMED   9419374
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF019566.1.
            
            Summary: This gene encodes a hypothalamic neuropeptide precursor
            protein that gives rise to two mature neuropeptides, orexin A and
            orexin B, by proteolytic processing. Orexin A and orexin B, which
            bind to orphan G-protein coupled receptors Hcrtr1 and Hcrtr2,
            function in the regulation of sleep and arousal. This neuropeptide
            arrangement may also play a role in feeding behavior, metabolism,
            and homeostasis. [provided by RefSeq, Sep 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF019566.1, CD769124.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..130
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 63.6 cM"
     Protein         1..130
                     /product="hypocretin neuropeptide precursor precursor"
                     /note="prepro-orexin; orexin A; orexin B;
                     preprohypocretin; hypocretin neuropeptide"
                     /calculated_mol_wt=10146
     Region          1..129
                     /region_name="Orexin"
                     /note="Prepro-orexin; pfam02072"
                     /db_xref="CDD:460436"
     sig_peptide     1..32
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=3375
     mat_peptide     33..65
                     /product="Orexin-A. /id=PRO_0000020264"
                     /note="propagated from UniProtKB/Swiss-Prot (O55241.1)"
                     /calculated_mol_wt=3583
     Site            33
                     /site_type="pyrrolidone-carboxylic-acid"
                     /note="Pyrrolidone carboxylic acid.
                     /evidence=ECO:0000250|UniProtKB:O55232; propagated from
                     UniProtKB/Swiss-Prot (O55241.1)"
     Site            65
                     /site_type="amidation"
                     /note="Leucine amide.
                     /evidence=ECO:0000250|UniProtKB:O55232; propagated from
                     UniProtKB/Swiss-Prot (O55241.1)"
     mat_peptide     69..96
                     /product="Orexin-B. /id=PRO_0000020265"
                     /note="propagated from UniProtKB/Swiss-Prot (O55241.1)"
                     /calculated_mol_wt=2937
     Site            96
                     /site_type="amidation"
                     /note="Methionine amide.
                     /evidence=ECO:0000250|UniProtKB:O55232; propagated from
                     UniProtKB/Swiss-Prot (O55241.1)"
     CDS             1..130
                     /gene="Hcrt"
                     /gene_synonym="PPOX"
                     /coded_by="NM_010410.2:91..483"
                     /db_xref="CCDS:CCDS25436.1"
                     /db_xref="GeneID:15171"
                     /db_xref="MGI:MGI:1202306"
ORIGIN      
        1 mnfpstkvpw aavtllllll lppallslgv daqplpdccr qktcscrlye llhgagnhaa
       61 giltlgkrrp gppglqgrlq rllqangnha agiltmgrra gaelephpcs grgcptvttt
      121 alaprggsgv
//
