LOCUS       NM_008226               3195 bp    mRNA    linear   ROD 11-OCT-2024
DEFINITION  Mus musculus hyperpolarization-activated, cyclic nucleotide-gated
            K+ 2 (Hcn2), mRNA.
ACCESSION   NM_008226 XM_985431
VERSION     NM_008226.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Han,X., Pinto,L.G., Vilar,B. and McNaughton,P.A.
  TITLE     Opioid-Induced Hyperalgesia and Tolerance Are Driven by HCN Ion
            Channels
  JOURNAL   J Neurosci 44 (6), e1368232023 (2024)
   PUBMED   38124021
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Zheng,Y., Shao,S., Zhang,Y., Yuan,S., Xing,Y., Wang,J., Qi,X.,
            Cui,K., Tong,J., Liu,F., Cui,S., Wan,Y. and Yi,M.
  TITLE     HCN2 Channels in the Ventral Hippocampal CA1 Regulate Nociceptive
            Hypersensitivity in Mice
  JOURNAL   Int J Mol Sci 24 (18), 13823 (2023)
   PUBMED   37762124
  REMARK    GeneRIF: HCN2 Channels in the Ventral Hippocampal CA1 Regulate
            Nociceptive Hypersensitivity in Mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3195)
  AUTHORS   Yan,Y., Zhu,M., Cao,X., Xu,G., Shen,W., Li,F., Zhang,J., Luo,L.,
            Zhang,X., Zhang,D. and Liu,T.
  TITLE     Thalamocortical Circuit Controls Neuropathic Pain via Up-regulation
            of HCN2 in the Ventral Posterolateral Thalamus
  JOURNAL   Neurosci Bull 39 (5), 774-792 (2023)
   PUBMED   36538279
  REMARK    GeneRIF: Thalamocortical Circuit Controls Neuropathic Pain via
            Up-regulation of HCN2 in the Ventral Posterolateral Thalamus.
REFERENCE   4  (bases 1 to 3195)
  AUTHORS   Kwon,J., Choi,Y.I., Jo,H.J., Lee,S.H., Lee,H.K., Kim,H., Moon,J.Y.
            and Jung,S.J.
  TITLE     The Role of Prostaglandin E1 as a Pain Mediator through
            Facilitation of Hyperpolarization-Activated Cyclic Nucleotide-Gated
            Channel 2 via the EP2 Receptor in Trigeminal Ganglion Neurons of
            Mice
  JOURNAL   Int J Mol Sci 22 (24), 13534 (2021)
   PUBMED   34948328
  REMARK    GeneRIF: The Role of Prostaglandin E1 as a Pain Mediator through
            Facilitation of Hyperpolarization-Activated Cyclic Nucleotide-Gated
            Channel 2 via the EP2 Receptor in Trigeminal Ganglion Neurons of
            Mice.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3195)
  AUTHORS   Lyman,K.A., Han,Y., Zhou,C., Renteria,I., Besing,G.L., Kurz,J.E.
            and Chetkovich,D.M.
  TITLE     Hippocampal cAMP regulates HCN channel function on two time scales
            with differential effects on animal behavior
  JOURNAL   Sci Transl Med 13 (621), eabl4580 (2021)
   PUBMED   34818058
REFERENCE   6  (bases 1 to 3195)
  AUTHORS   Zong,X., Stieber,J., Ludwig,A., Hofmann,F. and Biel,M.
  TITLE     A single histidine residue determines the pH sensitivity of the
            pacemaker channel HCN2
  JOURNAL   J Biol Chem 276 (9), 6313-6319 (2001)
   PUBMED   11096117
REFERENCE   7  (bases 1 to 3195)
  AUTHORS   Chen,J., Mitcheson,J.S., Lin,M. and Sanguinetti,M.C.
  TITLE     Functional roles of charged residues in the putative voltage sensor
            of the HCN2 pacemaker channel
  JOURNAL   J Biol Chem 275 (46), 36465-36471 (2000)
   PUBMED   10962006
REFERENCE   8  (bases 1 to 3195)
  AUTHORS   Ludwig,A., Zong,X., Jeglitsch,M., Hofmann,F. and Biel,M.
  TITLE     A family of hyperpolarization-activated mammalian cation channels
  JOURNAL   Nature 393 (6685), 587-591 (1998)
   PUBMED   9634236
REFERENCE   9  (bases 1 to 3195)
  AUTHORS   Santoro,B., Liu,D.T., Yao,H., Bartsch,D., Kandel,E.R.,
            Siegelbaum,S.A. and Tibbs,G.R.
  TITLE     Identification of a gene encoding a hyperpolarization-activated
            pacemaker channel of brain
  JOURNAL   Cell 93 (5), 717-729 (1998)
   PUBMED   9630217
REFERENCE   10 (bases 1 to 3195)
  AUTHORS   Santoro,B., Grant,S.G., Bartsch,D. and Kandel,E.R.
  TITLE     Interactive cloning with the SH3 domain of N-src identifies a new
            brain specific ion channel protein, with homology to eag and cyclic
            nucleotide-gated channels
  JOURNAL   Proc Natl Acad Sci U S A 94 (26), 14815-14820 (1997)
   PUBMED   9405696
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC151828.3,
            AF064873.1 and CX242892.1.
            
            On or before May 4, 2006 this sequence version replaced
            XM_985431.1, NM_008226.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ225122.1, AB154248.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-586               AC151828.3         125008-125593
            587-1491            AF064873.1         106-1010
            1492-2161           CX242892.1         3-672
            2162-3195           AC151828.3         143449-144482
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..3195
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /note="hyperpolarization-activated, cyclic
                     nucleotide-gated K+ 2"
                     /db_xref="GeneID:15166"
                     /db_xref="MGI:MGI:1298210"
     exon            1..586
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..2627
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /note="HAC-1; BCNG-2; brain cyclic nucleotide-gated
                     channel 2; hyperpolarization-activated cation channel 1"
                     /codon_start=1
                     /product="potassium/sodium hyperpolarization-activated
                     cyclic nucleotide-gated channel 2"
                     /protein_id="NP_032252.1"
                     /db_xref="CCDS:CCDS23986.1"
                     /db_xref="GeneID:15166"
                     /db_xref="MGI:MGI:1298210"
                     /translation="MDARGGGGRPGDSPGTTPAPGPPPPPPPPAPPQPQPPPAPPPNP
                     TTPSHPESADEPGPRARLCSRDSACTPGAAKGGANGECGRGEPQCSPEGPARGPKVSF
                     SCRGAASGPSAAEEAGSEEAGPAGEPRGSQASFLQRQFGALLQPGVNKFSLRMFGSQK
                     AVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWI
                     VFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDY
                     IFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLA
                     SAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPSDCWVSINNMVNHSWSELYSFALF
                     KAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQ
                     EKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNC
                     RKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLT
                     KGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRA
                     FETVAIDRLDRIGKKNSILLHKVQHDLSSGVFNNQENAIIQEIVKYDREMVQQAELGQ
                     RVGLFPPPPPPQVTSAIATLQQAVAMSFCPQVARPLVGPLALGSPRLVRRAPPGPLPP
                     AASPGPPAASPPAAPSSPRAPRTSPYGVPGSPATRVGPALPARRLSRASRPLSASQPS
                     LPHGVPAPSPAASARPASSSTPRLGPAPTARTAAPSPDRRDSASPGAASGLDPLDSAR
                     SRLSSNL"
     exon            587..1010
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     exon            1011..1172
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     exon            1173..1391
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     exon            1392..1538
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1779
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1944
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
     exon            1945..3195
                     /gene="Hcn2"
                     /gene_synonym="BCNG2; HAC1; trls"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ccgctccgct ccgcactgcc cggcgccgcc tcgccatgga tgcgcgcggg ggcggcgggc
       61 ggccgggcga tagtccgggc acgacccctg cgccggggcc gccgccaccg ccgccgccgc
      121 ccgcgccccc tcagcctcag ccaccacccg cgccaccccc gaaccccacg accccctcgc
      181 acccggagtc ggcggacgag cccggcccgc gcgcccggct ctgcagccgc gacagcgcct
      241 gcacccctgg cgcggccaag ggcggcgcga atggcgagtg cgggcgcggg gagccgcagt
      301 gcagccccga gggccccgcg cgcggcccca aggtttcgtt ctcatgccgc ggggcggcct
      361 ccgggccctc ggcggccgag gaggcgggca gcgaggaggc gggcccggcg ggtgagccgc
      421 gcggcagcca ggctagcttc ctgcagcgcc aattcggggc gcttctgcag cccggcgtca
      481 acaagttctc cctgcggatg ttcggcagcc agaaggccgt ggagcgcgag caggaacgcg
      541 tgaagtcggc gggggcctgg atcatccacc cctacagcga cttcaggttc tactgggact
      601 tcaccatgct gttgttcatg gtgggaaatc tcattatcat tcccgtgggc atcactttct
      661 tcaaggacga gaccaccgcg ccctggatcg tcttcaacgt ggtctcggac actttcttcc
      721 tcatggactt ggtgttgaac ttccgcaccg gcattgttat tgaggacaac acggagatca
      781 tcctggaccc cgagaagata aagaagaagt acttgcgtac gtggttcgtg gtggacttcg
      841 tgtcatccat cccggtggac tacatcttcc tcatagtgga gaagggaatc gactccgagg
      901 tctacaagac agcgcgtgct ctgcgcatcg tgcgcttcac caagatcctc agtctgctgc
      961 ggctgctgcg gctatcacgg ctcatccgat atatccacca gtgggaagag attttccaca
     1021 tgacctacga cctggcaagt gcagtgatgc gcatctgtaa cctgatcagc atgatgctac
     1081 tgctctgcca ctgggacggt tgcctgcagt tcctggtgcc catgctgcaa gacttcccca
     1141 gcgactgctg ggtgtccatc aacaacatgg tgaaccactc gtggagcgag ctctactcgt
     1201 tcgcgctctt caaggccatg agccacatgc tgtgcatcgg ctacgggcgg caggcgcccg
     1261 agagcatgac agacatctgg ctgaccatgc tcagcatgat cgtaggcgcc acctgctatg
     1321 ccatgttcat tgggcacgcc actgcgctca tccagtccct ggattcgtca cggcgccaat
     1381 accaggagaa gtacaagcaa gtagagcaat acatgtcctt ccacaaactg cccgctgact
     1441 tccgccagaa gatccacgat tactatgaac accggtacca agggaagatg tttgatgagg
     1501 acagcatcct tggggaactc aacgggccac tgcgtgagga gattgtgaac ttcaactgcc
     1561 ggaagctggt ggcttccatg ccgctgtttg ccaatgcaga ccccaacttc gtcacagcca
     1621 tgctgacaaa gctcaaattt gaggtcttcc agcctggaga ttacatcatc cgagagggga
     1681 ccatcgggaa gaagatgtac ttcatccagc atggggtggt gagcgtgctc accaagggca
     1741 acaaggagat gaagctgtcg gatggctcct atttcgggga gatctgcttg ctcacgaggg
     1801 gccggcgtac ggccagcgtg cgagctgaca cctactgtcg cctctactca ctgagtgtgg
     1861 acaatttcaa cgaggtgctg gaggaatacc ccatgatgcg gcgtgccttt gagactgtgg
     1921 ctattgaccg gctagatcgc ataggcaaga agaactccat cttgctgcac aaggttcagc
     1981 atgatctcag ctcaggtgtg ttcaacaacc aggagaatgc catcatccag gagattgtca
     2041 aatatgaccg tgagatggtg cagcaggcag agcttggcca gcgtgtgggg ctcttcccac
     2101 caccgccacc accgcaggtc acatcggcca ttgccaccct acagcaggct gtggccatga
     2161 gcttctgccc gcaggtggcc cgcccgctcg tggggcccct ggcgctaggc tccccacgcc
     2221 tagtgcgccg cgcgccccca gggcctctgc ctcctgcagc ctcgccaggg ccacccgcag
     2281 caagcccccc ggctgcaccc tcgagccctc gggcaccgcg gacctcaccc tacggtgtgc
     2341 ctggctctcc ggcaacgcgt gtggggcccg cattgcccgc acgtcgcctg agccgcgcct
     2401 cgcgcccact gtccgcctcg cagccctcgc tgccccatgg cgtgcccgcg cccagccccg
     2461 cggcctctgc gcgcccggcc agcagctcca cgccgcgcct gggacccgca cccaccgccc
     2521 ggaccgccgc gcccagtccg gaccgcaggg actcagcctc gccgggcgct gccagtggcc
     2581 tcgacccact ggactctgcg cgctcgcgcc tctcttccaa cttgtgaccc ttgagcgccg
     2641 ccccgcgggc cgggcggggc cgtcatccac accaaagcca tgcctcgcgc cgcccgcccg
     2701 tgcccgtgca gaagccatag agggacgtag gtagcttagg aggcgggcgg ccctgcgccc
     2761 ggctgtcccc ccatcgccct gcgcccaccc ccatcgcccc tgccccagcg gcggccgcac
     2821 gggagaggga ggggtgcgat cacctcggtg cctcagcccc aacctgggac agggacaggg
     2881 cggccctggc cgaggacctg gctgtgcccc gcatgtgcgg tggcctccga ggaagaatat
     2941 ggatcaagtg caatactcgg cccgccggct tcccgctgcc cctggcaagc tcacgcaata
     3001 accagcccgc ccctgcccac gcgtccgtgg tgacctccct cgggcaccca ggggcgggct
     3061 tcacggccaa gccggcgtgg gggtgaggct gggtccccgc cgtcgccatg aatgtactga
     3121 cgagccgagg cagcagtggc ccccacgccc cattaaccca caaccccatt ccgcgcaata
     3181 aacgacagca ttggc
//
