LOCUS       NM_008225               2031 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus hematopoietic cell specific Lyn substrate 1 (Hcls1),
            mRNA.
ACCESSION   NM_008225
VERSION     NM_008225.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Blumenthal,D., Chandra,V., Avery,L. and Burkhardt,J.K.
  TITLE     Mouse T cell priming is enhanced by maturation-dependent stiffening
            of the dendritic cell cortex
  JOURNAL   Elife 9, e55995 (2020)
   PUBMED   32720892
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Bendell,A.C., Williamson,E.K., Chen,C.S., Burkhardt,J.K. and
            Hammer,D.A.
  TITLE     The Arp2/3 complex binding protein HS1 is required for efficient
            dendritic cell random migration and force generation
  JOURNAL   Integr Biol (Camb) 9 (8), 695-708 (2017)
   PUBMED   28678266
  REMARK    GeneRIF: HS1 and Arp2/3 complex-mediated actin polymerization are
            essential for the most efficient dendritic cell random migration
            and force generation.
REFERENCE   3  (bases 1 to 2031)
  AUTHORS   Latasiewicz,J., Artz,A., Jing,D., Blanco,M.P., Currie,S.M.,
            Avila,M.V., Schnoor,M. and Vestweber,D.
  TITLE     HS1 deficiency impairs neutrophil recruitment in vivo and
            activation of the small GTPases Rac1 and Rap1
  JOURNAL   J Leukoc Biol 101 (5), 1133-1142 (2017)
   PUBMED   28122813
  REMARK    GeneRIF: HS1 as an important regulator of proper Rac1 and Rap1
            activation and neutrophil extravasation.
REFERENCE   4  (bases 1 to 2031)
  AUTHORS   Liepelt,A., Naarmann-de Vries,I.S., Simons,N., Eichelbaum,K.,
            Fohr,S., Archer,S.K., Castello,A., Usadel,B., Krijgsveld,J.,
            Preiss,T., Marx,G., Hentze,M.W., Ostareck,D.H. and
            Ostareck-Lederer,A.
  TITLE     Identification of RNA-binding Proteins in Macrophages by
            Interactome Capture
  JOURNAL   Mol Cell Proteomics 15 (8), 2699-2714 (2016)
   PUBMED   27281784
  REMARK    GeneRIF: Results identified two proteins P23 and HCLS1, which were
            not known as RNA-binding proteins, exhibiting RNA-binding activity.
REFERENCE   5  (bases 1 to 2031)
  AUTHORS   Habif,G., Grasset,M.F., Kieffer-Jaquinod,S., Kuhn,L., Mouchiroud,G.
            and Gobert-Gosse,S.
  TITLE     Phosphoproteome analyses reveal specific implications of Hcls1,
            p21-activated kinase 1 and Ezrin in proliferation of a myeloid
            progenitor cell line downstream of wild-type and ITD mutant
            Fms-like tyrosine kinase 3 receptors
  JOURNAL   J Proteomics 78, 231-244 (2013)
   PUBMED   23017497
  REMARK    GeneRIF: Phosphoproteome analyses reveal specific implications of
            Hcls1, p21-activated kinase 1 and Ezrin in proliferation of a
            myeloid progenitor cell line downstream of wild-type and ITD mutant
            Fms-like tyrosine kinase 3 receptors.
REFERENCE   6  (bases 1 to 2031)
  AUTHORS   Takemoto,Y., Sato,M., Furuta,M. and Hashimoto,Y.
  TITLE     Distinct binding patterns of HS1 to the Src SH2 and SH3 domains
            reflect possible mechanisms of recruitment and activation of
            downstream molecules
  JOURNAL   Int Immunol 8 (11), 1699-1705 (1996)
   PUBMED   8943564
REFERENCE   7  (bases 1 to 2031)
  AUTHORS   Taniuchi,I., Kitamura,D., Maekawa,Y., Fukuda,T., Kishi,H. and
            Watanabe,T.
  TITLE     Antigen-receptor induced clonal expansion and deletion of
            lymphocytes are impaired in mice lacking HS1 protein, a substrate
            of the antigen-receptor-coupled tyrosine kinases
  JOURNAL   EMBO J 14 (15), 3664-3678 (1995)
   PUBMED   7641686
REFERENCE   8  (bases 1 to 2031)
  AUTHORS   Takemoto,Y., Furuta,M., Li,X.K., Strong-Sparks,W.J. and
            Hashimoto,Y.
  TITLE     LckBP1, a proline-rich protein expressed in haematopoietic lineage
            cells, directly associates with the SH3 domain of protein tyrosine
            kinase p56lck
  JOURNAL   EMBO J 14 (14), 3403-3414 (1995)
   PUBMED   7628441
REFERENCE   9  (bases 1 to 2031)
  AUTHORS   Kitamura,D., Kaneko,H., Taniuchi,I., Akagi,K., Yamamura,K. and
            Watanabe,T.
  TITLE     Molecular cloning and characterization of mouse HS1
  JOURNAL   Biochem Biophys Res Commun 208 (3), 1137-1146 (1995)
   PUBMED   7535527
REFERENCE   10 (bases 1 to 2031)
  AUTHORS   Fukamachi,H., Yamada,N., Miura,T., Kato,T., Ishikawa,M.,
            Gulbins,E., Altman,A., Kawakami,Y. and Kawakami,T.
  TITLE     Identification of a protein, SPY75, with repetitive
            helix-turn-helix motifs and an SH3 domain as a major substrate for
            protein tyrosine kinase(s) activated by Fc epsilon RI cross-linking
  JOURNAL   J Immunol 152 (2), 642-652 (1994)
   PUBMED   7506729
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154763.2, AK040049.1, CA321698.1 and BX519502.1.
            
            On Aug 12, 2009 this sequence version replaced NM_008225.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC007469.1, X84797.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC154763.2         68725-68737
            14-510              AK040049.1         2-498
            511-546             AC154763.2         84894-84929
            547-657             AC154763.2         89002-89112
            658-713             AC154763.2         89706-89761
            714-783             AC154763.2         90313-90382
            784-918             AC154763.2         90954-91088
            919-1579            CA321698.1         1-661
            1580-1744           AC154763.2         96518-96682
            1745-2031           BX519502.1         1-287
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 26.3 cM"
     gene            1..2031
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /note="hematopoietic cell specific Lyn substrate 1"
                     /db_xref="GeneID:15163"
                     /db_xref="MGI:MGI:104568"
     exon            1..92
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             93..1553
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /note="lckBP1; hematopoietic cell-specific LYN substrate
                     1"
                     /codon_start=1
                     /product="hematopoietic lineage cell-specific protein"
                     /protein_id="NP_032251.2"
                     /db_xref="CCDS:CCDS37336.1"
                     /db_xref="GeneID:15163"
                     /db_xref="MGI:MGI:104568"
                     /translation="MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIE
                     GSGRTEHINIHQLRNKVSEEHDILKKKELESGPKASHGYGGRFGVERDRMDKSAVGHE
                     YVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDYKGEVEKHASQKDYSHGFGGR
                     YGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAP
                     TTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSR
                     EVQQPSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSP
                     LQNHLEDNEEPPALPPRTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVG
                     ELDRQDEDAEGDYEDVLEPEDTPSLSYQAGPSAGAGGAGISAIALYDYQGEGSDELSF
                     DPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL"
     exon            93..176
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            177..250
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            251..380
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            381..491
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            492..546
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            547..657
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            658..713
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            714..783
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            784..995
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1112
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1113..1356
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1419
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..2018
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1997..2002
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /note="hexamer: AATAAA"
     polyA_site      2018
                     /gene="Hcls1"
                     /gene_synonym="HS1"
                     /note="major polyA site"
ORIGIN      
        1 gagcagctgc tctgttactt accctgaagt ggcagccgct ggagggggcg cctggtgtag
       61 atgtgaaaag ccgtaaccag gaaccagtaa agatgtggaa gtctgtagtg gggcatgatg
      121 tatcggtttc cgtggagacc cagggtgatg actgggatac agaccctgac tttgtgaatg
      181 acatctccga gaaggagcaa cggtggggag ccaagaccat tgagggctct ggacgcacag
      241 agcacatcaa catccaccag ctgaggaaca aagtgtcaga ggagcacgac atcctcaaga
      301 agaaggagct ggaatcgggg cctaaggcat cccatggcta tggcggtcgg tttggagtgg
      361 agagagaccg gatggacaag agtgccgtgg gccacgagta tgttgctgat gtggagaaac
      421 actcatctca gactgatgcc gccagaggct ttgggggcaa atatggagtt gagagggacc
      481 gggcagacaa gtcagcggtg ggctttgact acaaaggaga agtggaaaag catgcatctc
      541 agaaagatta ctctcatggc tttggtggcc gctacggggt agagaaggat aaacgggaca
      601 aagcagccct gggatacgac tacaaaggag agacggagaa gcacgagtct cagagagatt
      661 atgccaaggg ctttggtggc caatatggaa tccagaaaga ccgagtggat aagagtgctg
      721 ttggcttcaa tgaaatggag gccccaacca cggcgtataa gaagacaaca cccatagaag
      781 ctgcttccag tggtgcccgt gggctgaagg caaaatttga gtccctggct gaggagaaga
      841 ggaagcgaga ggaagaagag aaggcacagc agatggccag gcagcaacag gagcgaaagg
      901 ctgtggtaaa gatgagccga gaagtccagc agccatccat gcctgtggaa gagccagcgg
      961 caccagccca gttgcccaag aagatctcct cagaggtctg gcctccagca gagagtcacc
     1021 taccgccaga gtctcagcca gtgagaagca gaagggaata ccctgtgccc tctctgccca
     1081 cgaggcagtc tccattgcag aatcacttgg aggacaacga ggagccccca gctctgcccc
     1141 ctaggacccc agaaggcctc caggtggtgg aagagccagt gtacgaagca gcacccgagc
     1201 tggagccgga gccagagcct gactatgagc cagagccaga gacagagcct gactatgagg
     1261 atgttgggga gttagatcgg caggatgagg atgcagaggg agactatgag gatgtgctgg
     1321 agcccgagga caccccttct ctgtcctacc aagctggacc ctcagctggg gctggtggtg
     1381 cggggatctc tgctatagcc ctgtatgatt accaaggaga gggaagcgat gagctttcct
     1441 ttgatccaga tgacatcatc actgacattg agatggtgga tgaaggctgg tggcggggcc
     1501 aatgccgtgg ccactttgga cttttccctg caaactatgt caagctcctc taatgaccag
     1561 cccattgtct tccgacttcc cgaattcgaa gctgctctgc ctccctcttc ccactccatg
     1621 gtactgctgc aaggacctgg ctgaacatca tgagatgcct gaagttctgg cagtctgtct
     1681 cccgcctctt taagagcttt aggtagaatc gctccaggtg ggggtggggg tgggggtggg
     1741 atccctctgt ccctctgtga ccactcttcc ctgaggtagc tcatgaaatc atcttgcaga
     1801 cctgcctcct tcagccgcac cccagctctg ccaaccttgc tctagagtgc tgggattccc
     1861 ttgccccgac cctgggtgcc agcctagagg ggaggctctc acagggctgc ctgattcgcc
     1921 ctgttgtgct tttgctcatt tttcttccct tagcagacaa attggaactg cccttctgtt
     1981 tagtcctaaa actgaaaata aaatgagact gtggctaaaa aaaaaaaaaa a
//
