LOCUS       NP_034537                524 aa            linear   ROD 01-JUN-2024
DEFINITION  tyrosine-protein kinase HCK isoform p59Hck [Mus musculus].
ACCESSION   NP_034537 XP_011250106
VERSION     NP_034537.2
DBSOURCE    REFSEQ: accession NM_010407.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 524)
  AUTHORS   Chen,M., Menon,M.C., Wang,W., Fu,J., Yi,Z., Sun,Z., Liu,J., Li,Z.,
            Mou,L., Banu,K., Lee,S.W., Dai,Y., Anandakrishnan,N.,
            Azeloglu,E.U., Lee,K., Zhang,W., Das,B., He,J.C. and Wei,C.
  TITLE     HCK induces macrophage activation to promote renal inflammation and
            fibrosis via suppression of autophagy
  JOURNAL   Nat Commun 14 (1), 4297 (2023)
   PUBMED   37463911
  REMARK    GeneRIF: HCK induces macrophage activation to promote renal
            inflammation and fibrosis via suppression of autophagy.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 524)
  AUTHORS   Futosi,K., Bajza,B., Deli,D., Erdelyi,A., Tusnady,S. and Mocsai,A.
  TITLE     Analysis of intracellular tyrosine phosphorylation in circulating
            neutrophils as a rapid assay for the in vivo effect of oral
            tyrosine kinase inhibitors
  JOURNAL   Front Pharmacol 14, 1056154 (2023)
   PUBMED   37089957
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 524)
  AUTHORS   Lowell,C.A., Fumagalli,L. and Berton,G.
  TITLE     Deficiency of Src family kinases p59/61hck and p58c-fgr results in
            defective adhesion-dependent neutrophil functions
  JOURNAL   J Cell Biol 133 (4), 895-910 (1996)
   PUBMED   8666673
REFERENCE   4  (residues 1 to 524)
  AUTHORS   Lowell,C.A., Niwa,M., Soriano,P. and Varmus,H.E.
  TITLE     Deficiency of the Hck and Src tyrosine kinases results in extreme
            levels of extramedullary hematopoiesis
  JOURNAL   Blood 87 (5), 1780-1792 (1996)
   PUBMED   8634424
REFERENCE   5  (residues 1 to 524)
  AUTHORS   Avraham,K.B., Prezioso,V.R., Chen,W.S., Lai,E., Sladek,F.M.,
            Zhong,W., Darnell,J.E. Jr., Jenkins,N.A. and Copeland,N.G.
  TITLE     Murine chromosomal location of four hepatocyte-enriched
            transcription factors: HNF-3 alpha, HNF-3 beta, HNF-3 gamma, and
            HNF-4
  JOURNAL   Genomics 13 (2), 264-268 (1992)
   PUBMED   1612587
REFERENCE   6  (residues 1 to 524)
  AUTHORS   Jenkins,N.A., Mattei,M.G., Gilbert,D.J., Linard,C.G., Mbikay,M.,
            Chretien,M. and Copeland,N.G.
  TITLE     Assignment of secretogranin I locus to mouse chromosome 2 by in
            situ hybridization and interspecific backcross analysis
  JOURNAL   Genomics 11 (2), 479-480 (1991)
   PUBMED   1685143
REFERENCE   7  (residues 1 to 524)
  AUTHORS   Lock,P., Ralph,S., Stanley,E., Boulet,I., Ramsay,R. and Dunn,A.R.
  TITLE     Two isoforms of murine hck, generated by utilization of alternative
            translational initiation codons, exhibit different patterns of
            subcellular localization
  JOURNAL   Mol Cell Biol 11 (9), 4363-4370 (1991)
   PUBMED   1875927
  REMARK    GeneRIF: Alternate protein isoforms arise through the use of a
            non-AUG (CUG), and a downstream in-frame AUG, translation
            initiation codons, in mouse and human.
REFERENCE   8  (residues 1 to 524)
  AUTHORS   Lock,P., Stanley,E., Holtzman,D.A. and Dunn,A.R.
  TITLE     Functional analysis and nucleotide sequence of the promoter region
            of the murine hck gene
  JOURNAL   Mol Cell Biol 10 (9), 4603-4611 (1990)
   PUBMED   2388619
REFERENCE   9  (residues 1 to 524)
  AUTHORS   Holtzman,D.A., Cook,W.D. and Dunn,A.R.
  TITLE     Isolation and sequence of a cDNA corresponding to a src-related
            gene expressed in murine hemopoietic cells
  JOURNAL   Proc Natl Acad Sci U S A 84 (23), 8325-8329 (1987)
   PUBMED   3317404
REFERENCE   10 (residues 1 to 524)
  AUTHORS   Klemsz,M.J., McKercher,S.R. and Maki,R.A.
  TITLE     Nucleotide sequence of the mouse hck gene
  JOURNAL   Nucleic Acids Res 15 (22), 9600 (1987)
   PUBMED   3684607
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY157889.1, AK150290.1 and
            AI849071.1.
            
            On or before Feb 15, 2015 this sequence version replaced
            XP_011250106.1, NP_034537.1.
            
            Summary: The protein encoded by this gene is a member of the Src
            family of tyrosine kinases. This protein is primarily hemopoietic,
            particularly in cells of the myeloid and B-lymphoid lineages. It
            may play a role in the innate immune response and the STAT5
            signaling pathway. Alternative translation initiation site usage,
            including a non-AUG (CUG) codon, results in the production of two
            different isoforms, that have different subcellular localization.
            [provided by RefSeq, Feb 2010].
            
            Transcript Variant: This transcript (1) encodes multiple isoforms
            due to the use of alternative translation initiation codons, as
            demonstrated in PMID:1875927. The longer isoform (p59Hck) is
            derived from an upstream non-AUG (CUG) start codon, while the
            shorter isoform (p56Hck) is derived from a downstream AUG start
            codon. The longer isoform (p59Hck) is represented in this RefSeq.
            
            CCDS Note: This CCDS ID represents the longer mouse Hck isoform,
            known as p59Hck, as described in PMID:1875927. This isoform
            initiates translation from a non-AUG (CUG) start codon that is
            well-conserved and present in a strong Kozak signal context.
            Alternative translation initiation from a downstream AUG start
            codon produces an isoform that is 21 aa shorter at the N-terminus.
            The shorter isoform, which is known as p56Hck, is represented by
            CCDS 50756.1. These isoforms exhibit distinct subcellular
            localization, as indicated in PMID:1875927.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK150709.1, AK150290.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            non-AUG initiation codon :: PMID: 1875927
            RefSeq Select criteria   :: based on conservation, expression,
                                        longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.41 cM"
     Protein         1..524
                     /product="tyrosine-protein kinase HCK isoform p59Hck"
                     /EC_number="2.7.10.2"
                     /note="B cell/myeloid kinase; tyrosine-protein kinase HCK;
                     p56-HCK/p59-HCK; hematopoietic cell kinase"
                     /calculated_mol_wt=58998
     Region          22
                     /region_name="Alternative AUG translation initiation
                     codon; isoform p56Hck"
     Site            50
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000269|PubMed:10934191; propagated from
                     UniProtKB/Swiss-Prot (P08103.4)"
     Region          80..135
                     /region_name="SH3"
                     /note="Src Homology 3 domain superfamily; cl17036"
                     /db_xref="CDD:473055"
     Site            order(85,87,90,94,111..112,127,129..130)
                     /site_type="other"
                     /note="peptide ligand binding site [polypeptide binding]"
                     /db_xref="CDD:212690"
     Region          138..241
                     /region_name="SH2_Src_HCK"
                     /note="Src homology 2 (SH2) domain found in HCK; cd10363"
                     /db_xref="CDD:198226"
     Site            order(149,169,171..174,179,195,197,209..211,230)
                     /site_type="other"
                     /note="autoinhibitory site [polypeptide binding]"
                     /db_xref="CDD:198226"
     Site            order(149,169,195,197)
                     /site_type="other"
                     /note="phosphotyrosine binding pocket [polypeptide
                     binding]"
                     /db_xref="CDD:198226"
     Site            order(196,224)
                     /site_type="other"
                     /note="hydrophobic binding pocket [polypeptide binding]"
                     /db_xref="CDD:198226"
     Site            200
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P08631; propagated from
                     UniProtKB/Swiss-Prot (P08103.4)"
     Site            207
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:19144319; propagated from
                     UniProtKB/Swiss-Prot (P08103.4)"
     Region          252..522
                     /region_name="Protein Kinases, catalytic domain"
                     /note="The protein kinase superfamily is mainly composed
                     of the catalytic domains of serine/threonine-specific and
                     tyrosine-specific protein kinases. It also includes RIO
                     kinases, which are atypical serine protein kinases,
                     aminoglycoside phosphotransferases; cl21453"
                     /db_xref="CDD:473864"
     Site            order(266..269,272,274,286,288,316,331..334,379,383..384,
                     386,396..397)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:270870"
     Site            409
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine, by autocatalysis.
                     /evidence=ECO:0000269|PubMed:10934191; propagated from
                     UniProtKB/Swiss-Prot (P08103.4)"
     Site            460
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P08631; propagated from
                     UniProtKB/Swiss-Prot (P08103.4)"
     Site            520
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:19144319; propagated from
                     UniProtKB/Swiss-Prot (P08103.4)"
     CDS             1..524
                     /gene="Hck"
                     /gene_synonym="Bmk"
                     /coded_by="NM_010407.4:226..1800"
                     /note="isoform p59Hck is encoded by transcript variant 1;
                     non-AUG (CUG) translation initiation codon"
                     /db_xref="CCDS:CCDS38284.1"
                     /db_xref="GeneID:15162"
                     /db_xref="MGI:MGI:96052"
ORIGIN      
        1 mggrsscedp gcprsegrap rmgcvksrfl rdgskaskte psanqkgpvy vpdptssskl
       61 gpnnsnsmpp gfvegsedti vvalydyeai hredlsfqkg dqmvvleeag ewwkarslat
      121 kkegyipsny varvnslete ewffkgisrk daerhllapg nmlgsfmird settkgsysl
      181 svrdfdpqhg dtvkhykirt ldsggfyisp rstfsslqel vlhykkgkdg lcqklsvpcv
      241 spkpqkpwek daweipresl qmekklgagq fgevwmatyn khtkvavktm kpgsmsveaf
      301 laeanlmksl qhdklvklha vvsqepifiv tefmakgsll dflkseegsk qplpklidfs
      361 aqisegmafi eqrnyihrdl raanilvsas lvckiadfgl ariiedneyt aregakfpik
      421 wtapeainfg sftiksdvws fgillmeivt ygripypgms npeviraleh gyrmprpdnc
      481 peelynimir cwknrpeerp tfeyiqsvld dfytatesqy qqqp
//
