LOCUS       NM_175520               3265 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus hydrocarboxylic acid receptor 1 (Hcar1), mRNA.
ACCESSION   NM_175520
VERSION     NM_175520.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Modaresinejad,M., Yang,X., Mohammad Nezhady,M.A., Zhu,T., Bajon,E.,
            Hou,X., Tahiri,H., Hardy,P., Rivera,J.C., Lachapelle,P. and
            Chemtob,S.
  TITLE     Endoplasmic Reticulum Stress Delays Choroid Development in the
            HCAR1 Knockout Mouse
  JOURNAL   Am J Pathol 194 (12), 2382-2397 (2024)
   PUBMED   39332673
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   3  (bases 1 to 3265)
  AUTHORS   Zhi,Y., Fan,K., Liu,S., Hu,K., Zan,X., Lin,L., Yang,Y., Gong,X.,
            Chen,K., Tang,L., Li,L., Huang,J., Zhang,S. and Zhang,L.
  TITLE     Deletion of GPR81 activates CREB/Smad7 pathway and alleviates liver
            fibrosis in mice
  JOURNAL   Mol Med 30 (1), 99 (2024)
   PUBMED   38982366
  REMARK    GeneRIF: Deletion of GPR81 activates CREB/Smad7 pathway and
            alleviates liver fibrosis in mice.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3265)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 3265)
  AUTHORS   Mohammad Nezhady,M.A., Cagnone,G., Joyal,J.S. and Chemtob,S.
  TITLE     Lack of HCAR1, the lactate GPCR, signaling promotes autistic-like
            behavior
  JOURNAL   Cell Commun Signal 21 (1), 196 (2023)
   PUBMED   37940970
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3265)
  AUTHORS   Jerome-Majewska,L.A., Achkar,T., Luo,L., Lupu,F. and Lacy,E.
  TITLE     The trafficking protein Tmed2/p24beta(1) is required for
            morphogenesis of the mouse embryo and placenta
  JOURNAL   Dev Biol 341 (1), 154-166 (2010)
   PUBMED   20178780
REFERENCE   7  (bases 1 to 3265)
  AUTHORS   Jeninga,E.H., Bugge,A., Nielsen,R., Kersten,S., Hamers,N., Dani,C.,
            Wabitsch,M., Berger,R., Stunnenberg,H.G., Mandrup,S. and
            Kalkhoven,E.
  TITLE     Peroxisome proliferator-activated receptor gamma regulates
            expression of the anti-lipolytic G-protein-coupled receptor 81
            (GPR81/Gpr81)
  JOURNAL   J Biol Chem 284 (39), 26385-26393 (2009)
   PUBMED   19633298
  REMARK    GeneRIF: Data show that siRNA-mediated knockdown of PPARgamma in
            differentiated 3T3-L1 adipocytes showed a significant decrease in
            Gpr81 protein expression.
REFERENCE   8  (bases 1 to 3265)
  AUTHORS   Liu,C., Wu,J., Zhu,J., Kuei,C., Yu,J., Shelton,J., Sutton,S.W.,
            Li,X., Yun,S.J., Mirzadegan,T., Mazur,C., Kamme,F. and
            Lovenberg,T.W.
  TITLE     Lactate inhibits lipolysis in fat cells through activation of an
            orphan G-protein-coupled receptor, GPR81
  JOURNAL   J Biol Chem 284 (5), 2811-2822 (2009)
   PUBMED   19047060
REFERENCE   9  (bases 1 to 3265)
  AUTHORS   Cai,T.Q., Ren,N., Jin,L., Cheng,K., Kash,S., Chen,R., Wright,S.D.,
            Taggart,A.K. and Waters,M.G.
  TITLE     Role of GPR81 in lactate-mediated reduction of adipose lipolysis
  JOURNAL   Biochem Biophys Res Commun 377 (3), 987-991 (2008)
   PUBMED   18952058
REFERENCE   10 (bases 1 to 3265)
  AUTHORS   Ge,H., Weiszmann,J., Reagan,J.D., Gupte,J., Baribault,H.,
            Gyuris,T., Chen,J.L., Tian,H. and Li,Y.
  TITLE     Elucidation of signaling and functional activities of an orphan
            GPCR, GPR81
  JOURNAL   J Lipid Res 49 (4), 797-803 (2008)
   PUBMED   18174606
  REMARK    GeneRIF: GPR81 couples to the Gi signaling pathway
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122753.11 and AC129582.10.
            
            On Dec 4, 2019 this sequence version replaced NM_175520.4.
            
            CCDS Note: The coding region has been updated to extend the
            N-terminus. The use of an alternative upstream start codon, which
            appears to be rodent-specific, would result in a protein that is 8
            aa longer at the N-terminal. The better conserved downstream AUG
            has a stronger Kozak signal than the uAUG, so it is possible that
            the downstream AUG may sometimes be used due to leaky scanning. No
            experimental evidence exists to show which AUG is preferentially
            used in vivo.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript is intronless :: AK029064.1, AK046700.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-595               AC122753.11        27318-27912         c
            596-3265            AC129582.10        1-2670
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 63.24 cM"
     gene            1..3265
                     /gene="Hcar1"
                     /gene_synonym="Gpr81"
                     /note="hydrocarboxylic acid receptor 1"
                     /db_xref="GeneID:243270"
                     /db_xref="MGI:MGI:2441671"
     exon            1..3265
                     /gene="Hcar1"
                     /gene_synonym="Gpr81"
                     /inference="alignment:Splign:2.1.0"
     CDS             402..1433
                     /gene="Hcar1"
                     /gene_synonym="Gpr81"
                     /note="G-protein coupled receptor 81"
                     /codon_start=1
                     /product="hydroxycarboxylic acid receptor 1"
                     /protein_id="NP_780729.3"
                     /db_xref="CCDS:CCDS19669.2"
                     /db_xref="GeneID:243270"
                     /db_xref="MGI:MGI:2441671"
                     /translation="MDNGSCCLIEGEPISQVMPPLLILVFVLGALGNGIALCGFCFHM
                     KTWKSSTIYLFNLAVADFLLMICLPLRTDYYLRRRHWIFGDIACRLVLFKLAMNRAGS
                     IVFLTVVAVDRYFKVVHPHHMVNAISNRTAAATACVLWTLVILGTVYLLMESHLCVQG
                     TLSSCESFIMESANGWHDVMFQLEFFLPLTIILFCSVNVVWSLRRRQQLTRQARMRRA
                     TRFIMVVASVFITCYLPSVLARLYFLWTVPTSACDPSVHTALHVTLSFTYLNSMLDPL
                     VYYFSSPSLPKFYTKLTICSLKPKRPGRTKTRRSEEMPISNLCSKSSIDGANRSQRPS
                     DGQWDLQVC"
     regulatory      3243..3248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hcar1"
                     /gene_synonym="Gpr81"
                     /note="hexamer: AATAAA"
     polyA_site      3265
                     /gene="Hcar1"
                     /gene_synonym="Gpr81"
                     /note="major polyA site"
ORIGIN      
        1 agagcattct gcagcgcaga gcgtgaggga aaaagacaaa accagaaaaa gaaaagtcat
       61 ctccagggct cgatctagca acgagtctgt agcatgtata gcgtcggacc cccgagctgc
      121 aacccagaaa tgtacactcg tgggaaaccg cttgcacccc agagcctgac ccagctgcag
      181 gcttcaactc tgtaggggac gtgcagctcg tgatccaagc ctaggagaaa ggacttgctg
      241 ccggctttca tttcctggct gaagtttctc tcgtgggtgc agcgcctgca tcccagggtg
      301 atgaggttag gggcccagct gctagaggag ccctagtgtt cggataggca gctgtgcctc
      361 tgtgccggcc accttggatg ccagtcctct ctccaactgc tatggacaac gggtcgtgct
      421 gtctcatcga gggggagccc atctcccaag tgatgcctcc tctactcatc ctggtcttcg
      481 tgcttggcgc cctgggcaac ggcatagccc tgtgcggctt ctgctttcac atgaagacct
      541 ggaagtcaag cactatttac cttttcaact tggctgtggc cgattttctc ctcatgatct
      601 gcttacccct tcggacagac tactacctca gacgcagaca ctggattttt ggagatatcg
      661 cctgtcgcct ggtcctcttc aagctggcca tgaatagggc cgggagcatt gtcttcctca
      721 ctgtggtggc tgtggatagg tatttcaaag tggtccaccc ccaccatatg gtgaatgcca
      781 tctccaaccg gactgccgcc gccaccgcct gtgtcctctg gactttggtc atcttgggga
      841 ctgtgtatct tctgatggag agtcacctgt gtgtgcaggg gacactgtcg tcctgtgaga
      901 gcttcatcat ggagtcagcc aacgggtggc acgatgtcat gttccagctg gagttcttcc
      961 tgcccctgac aatcatcttg ttctgctcgg tcaacgttgt ttggagcctg agacggaggc
     1021 agcagctgac cagacaggct cggatgagga gggccacccg gttcatcatg gtggtggctt
     1081 ctgtgttcat cacgtgttac ctgcccagcg tgctggctag gctctacttc ctctggacgg
     1141 tgcccactag tgcctgtgac ccctctgtcc acacagccct ccacgtcacc ctgagcttca
     1201 cctacctgaa cagtatgctg gatccccttg tatattactt ctcaagcccc tcgctcccca
     1261 aattctacac caagctcaca atctgcagcc tgaagcccaa acgcccagga cgcacgaaga
     1321 cgcggaggtc agaagagatg ccaatttcga acctctgcag taagagctcc atcgatgggg
     1381 caaatcgttc ccagaggcca tctgacgggc agtgggatct ccaagtgtgt tgaatgccat
     1441 taagacaaac agcccaacaa cgaggcagag aaatgggcaa tgtgagttaa atctgaaggg
     1501 tggaggactt gaagatgtcc cctcccactc ttagctgtat ctttctcact caggtagaaa
     1561 tgggatccac cctgcttgac cttttccaga aggttccaaa ccggttggtt gtgtttaaat
     1621 actctgatag caatggtgaa ggggcagcgt gtgagtgtga aggaaaccgt gggtgtcggg
     1681 ttaggaacta cctggagccc gtgtcgcttt gcatggctga gaaaagcggt atgagcctgg
     1741 ctgggtcttg ttctagctcg gagagagtta acgatctcaa taactcgtcg gtatttcctg
     1801 gactgaaaaa aatagaaact gcactgagtc aatacactta tttccagctg agcgagaccc
     1861 tttactgcag gacacccgga cctagccgtt ttttttaaat cttccctggg gagcctccac
     1921 acatttcaag gtttgaacat ccaggtggcc caggagggca gcaaaaagaa tctattctaa
     1981 ccttgctggc gccacgatat tttgctgatt ttaagtggtt tcatcctttg tttttctttt
     2041 gtttgcattt caaagaagat gctgagggac ttgtccacct gatatcagct atcgtttctc
     2101 cagtgggaac tgagagcctg tttacggcag caatggtggg gggggggtgc ttcctggtat
     2161 ttgggatggg ttaattcaag catggttgtt cttcactgct taatgcatga atttgagctg
     2221 aaatcctccc ttctcaagtc tttgtttaat ccacagtatg ttgtcccacc ctgtccagca
     2281 tcctcgtctg ttttgtcttt ggtgctgggc attgaacttg ggaccttatt cgagccaggc
     2341 aagcactgac cactgaactg cactcccaaa ccccttgtgc cccttttagc tgtagcgttg
     2401 ttagccaact tttgggagaa agcaaagcac tagaggtggc agcaacagtt tagctcaatg
     2461 tcctttcatc agtgtctaga cttctggtca gccatccggg tctcctattg ggggcctccc
     2521 tcaagcacat atgttccccc aaatactacc cagaattctc acagctaggt gattctgtga
     2581 aagtcccggc tgcccctgtc ctggagaagg agaaatagaa tccgtgttaa ccttagtccc
     2641 actttcaagc cacaaaagtg gtgacagcca ttcactctct ccagttccca gggtactctc
     2701 cccagggaag ggaccttgac atttatgtct aaagacataa attagatgct cctcaaggtt
     2761 gtccctgtgg cttcctttgc cagaggtgtt gaagcctagg tgcgaaaatc agtctgactg
     2821 cagggctggt gagaaggctc agtaggtaaa caggtctgcc gccaagccac caacctgagt
     2881 atccctgaga cccatgtggt aggagaacac caaatcccaa aggtggttct gtcctccacc
     2941 tatgttcacg catgcgcaca tgcacgcatg cgcacgcgcg cacacccacc caccaaatac
     3001 attaatgcaa taaaaatttt attggctaca cggtcaagtt tgaatcttag tttaaatgct
     3061 tattagacat gtgctcgtag ggaagacttt atttaacccc actcagtttt gatgttcagc
     3121 agggttaata ctgatgccaa agggtttggg agcaaattca atgactgtac actcataagc
     3181 atgagaaatc tgttgttccc aggtctgccc ggaagaagac catgtgcgtg tagtagttga
     3241 taaataaata gttgctgaac aacta
//
