LOCUS       NM_008220                646 bp    mRNA    linear   ROD 27-MAR-2020
DEFINITION  Mus musculus hemoglobin, beta adult t chain (Hbb-bt), mRNA.
ACCESSION   NM_008220
VERSION     NM_008220.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Ghanem LR, Kromer A, Silverman IM, Ji X, Gazzara M, Nguyen N,
            Aguilar G, Martinelli M, Barash Y and Liebhaber SA.
  TITLE     Poly(C)-Binding Protein Pcbp2 Enables Differentiation of Definitive
            Erythropoiesis by Directing Functional Splicing of the Runx1
            Transcript
  JOURNAL   Mol. Cell. Biol. 38 (16), e00175-18 (2018)
   PUBMED   29866654
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  AUTHORS   Dykes IM, van Bueren KL and Scambler PJ.
  TITLE     HIC2 regulates isoform switching during maturation of the
            cardiovascular system
  JOURNAL   J. Mol. Cell. Cardiol. 114, 29-37 (2018)
   PUBMED   29061339
REFERENCE   3  (bases 1 to 646)
  AUTHORS   Stauber M, Weidemann M, Dittrich-Breiholz O, Lobschat K, Alten L,
            Mai M, Beckers A, Kracht M and Gossler A.
  TITLE     Identification of FOXJ1 effectors during ciliogenesis in the foetal
            respiratory epithelium and embryonic left-right organiser of the
            mouse
  JOURNAL   Dev. Biol. 423 (2), 170-188 (2017)
   PUBMED   27914912
REFERENCE   4  (bases 1 to 646)
  AUTHORS   Runck AM, Moriyama H and Storz JF.
  TITLE     Evolution of duplicated beta-globin genes and the structural basis
            of hemoglobin isoform differentiation in Mus
  JOURNAL   Mol. Biol. Evol. 26 (11), 2521-2532 (2009)
   PUBMED   19675095
REFERENCE   5  (bases 1 to 646)
  AUTHORS   Storz JF, Baze M, Waite JL, Hoffmann FG, Opazo JC and Hayes JP.
  TITLE     Complex signatures of selection and gene conversion in the
            duplicated globin genes of house mice
  JOURNAL   Genetics 177 (1), 481-500 (2007)
   PUBMED   17660536
REFERENCE   6  (bases 1 to 646)
  AUTHORS   Appanah R, Dickerson DR, Goyal P, Groudine M and Lorincz MC.
  TITLE     An unmethylated 3' promoter-proximal region is required for
            efficient transcription initiation
  JOURNAL   PLoS Genet. 3 (2), e27 (2007)
   PUBMED   17305432
REFERENCE   7  (bases 1 to 646)
  AUTHORS   Bulger M, van Doorninck JH, Saitoh N, Telling A, Farrell C, Bender
            MA, Felsenfeld G, Axel R and Groudine M.
  TITLE     Conservation of sequence and structure flanking the mouse and human
            beta-globin loci: the beta-globin genes are embedded within an
            array of odorant receptor genes
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 96 (9), 5129-5134 (1999)
   PUBMED   10220430
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1999 Jul 6;96(14):8307. von
            Doorninck JH [corrected to van Doorninck JH]]
REFERENCE   8  (bases 1 to 646)
  AUTHORS   Hug BA, Wesselschmidt RL, Fiering S, Bender MA, Epner E, Groudine M
            and Ley TJ.
  TITLE     Analysis of mice containing a targeted deletion of beta-globin
            locus control region 5' hypersensitive site 3
  JOURNAL   Mol. Cell. Biol. 16 (6), 2906-2912 (1996)
   PUBMED   8649401
REFERENCE   9  (bases 1 to 646)
  AUTHORS   Fiering S, Epner E, Robinson K, Zhuang Y, Telling A, Hu M, Martin
            DI, Enver T, Ley TJ and Groudine M.
  TITLE     Targeted deletion of 5'HS2 of the murine beta-globin LCR reveals
            that it is not essential for proper regulation of the beta-globin
            locus
  JOURNAL   Genes Dev. 9 (18), 2203-2213 (1995)
   PUBMED   7557375
REFERENCE   10 (bases 1 to 646)
  AUTHORS   Weaver,S., Comer,M.B., Jahn,C.L., Hutchison,C.A. 3rd and
            Edgell,M.H.
  TITLE     The adult beta-globin genes of the 'single' type mouse C57BL
  JOURNAL   Cell 24 (2), 403-411 (1981)
   PUBMED   7237554
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK028067.1 and AK012551.1.
            
            On May 13, 2013 this sequence version replaced NM_008220.4.
            
            Summary: This gene encodes a beta polypeptide chain found in adult
            hemoglobin, which consists of a tetramer of two alpha chains and
            two beta chains, and which functions in the transport of oxygen to
            various peripheral tissues. This gene is one of a cluster of
            beta-hemoglobin genes that are distally regulated by a locus
            control region, and which are organized along the chromosome in the
            order of their developmental expression. In mouse, two major
            strain-specific haplotypes of the beta-globin gene cluster are
            found - a 'single' haplotype found in C57BL/-type strains, which
            includes two highly similar adult beta-globin genes, beta s and
            beta t, and a 'diffuse' haplotype found in strains such as BALB/c
            and 129Sv, which includes two somewhat diverse adult beta-globin
            genes, beta-major and beta-minor. This gene represents the beta t
            adult gene found in the 'single' haplotype. Primary chromosome 7 of
            the mouse reference genome assembly, which is derived from C57BL/6
            strain mice, represents the 'single' haplotype, while the 'diffuse'
            haplotype is represented in the reference genome collection by the
            BALB/c strain alternate contig, NT_095534.1. [provided by RefSeq,
            May 2013].
            
            CCDS Note: This gene represents the second of two adult-expressed
            beta-globin genes in the 'single' haplotype of the beta-globin
            cluster (PMID:7237554). The 'single' haplotype is found in
            C57BL/-type strains and includes two highly similar adult
            beta-globin genes, beta s and beta t, while the 'diffuse' haplotype
            is found in strains such as BALB/c and 129Sv, and includes two
            somewhat diverse adult beta-globin genes, beta-major and
            beta-minor. This CCDS is based on primary chromosome 7 of the mouse
            reference genome assembly, which represents the 'single' haplotype.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849375,
                              SAMN00849378 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-626               AK028067.1         3-628
            627-646             AK012551.1         626-645
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7"
     gene            1..646
                     /gene="Hbb-bt"
                     /gene_synonym="Beta-t"
                     /note="hemoglobin, beta adult t chain"
                     /db_xref="GeneID:101488143"
                     /db_xref="MGI:MGI:5474850"
     exon            1..146
                     /gene="Hbb-bt"
                     /gene_synonym="Beta-t"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..498
                     /gene="Hbb-bt"
                     /gene_synonym="Beta-t"
                     /note="beta t"
                     /codon_start=1
                     /product="hemoglobin, beta adult t chain"
                     /protein_id="NP_032246.2"
                     /db_xref="CCDS:CCDS40060.1"
                     /db_xref="GeneID:101488143"
                     /db_xref="MGI:MGI:5474850"
                     /translation="MVHLTDAEKAAVSGLWGKVNADEVGGEALGRLLVVYPWTQRYFD
                     SFGDLSSASAIMGNAKVKAHGKKVITAFNDGLNHLDSLKGTFASLSELHCDKLHVDPE
                     NFRLLGNMIVIVLGHHLGKDFTPAAQAAFQKVVAGVAAALAHKYH"
     exon            147..369
                     /gene="Hbb-bt"
                     /gene_synonym="Beta-t"
                     /inference="alignment:Splign:2.1.0"
     exon            370..630
                     /gene="Hbb-bt"
                     /gene_synonym="Beta-t"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 ttacgtttgc ttctgattct gttgtgttga cttgcaacct cagaaacaga catcatggtg
       61 cacctgactg atgctgagaa ggctgctgtc tctggcctgt ggggaaaggt gaacgccgat
      121 gaagttggtg gtgaggccct gggcaggctg ctggttgtct acccttggac ccagcggtac
      181 tttgatagct ttggagacct atcctctgcc tctgctatca tgggtaatgc caaagtgaag
      241 gcccatggca agaaagtgat aactgccttt aacgatggcc tgaatcactt ggacagcctc
      301 aagggcacct ttgccagcct cagtgagctc cactgtgaca agctgcatgt ggatcctgag
      361 aacttcaggc tcctgggcaa tatgatcgtg attgtgctgg gccaccacct gggcaaggat
      421 ttcacccccg ctgcacaggc tgccttccag aaggtggtgg ctggagtggc tgctgccctg
      481 gctcacaagt accactaagc cccttttctg ctattgtcta tgcacaaagg ttatatgtcc
      541 cctagagaaa aactgtcaat tgtggggaaa tgatgaagac ctttgggcat ctagctttta
      601 tctaataaat gatatttact gtcatctcaa aaaaaaaaaa aaaaaa
//
