LOCUS       NM_001331048            5855 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus hyaluronan synthase 3 (Has3), transcript variant 2,
            mRNA.
ACCESSION   NM_001331048 XM_006530699
VERSION     NM_001331048.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Cooley,A., Madhukaran,S., Stroebele,E., Colon Caraballo,M.,
            Wang,L., Akgul,Y., Hon,G.C. and Mahendroo,M.
  TITLE     Dynamic states of cervical epithelia during pregnancy and
            epithelial barrier disruption
  JOURNAL   iScience 26 (2), 105953 (2023)
   PUBMED   36718364
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5855)
  AUTHORS   Lin,X., Mekonnen,T., Verma,S., Zevallos-Delgado,C., Singh,M.,
            Aglyamov,S.R., Gesteira,T.F., Larin,K.V. and Coulson-Thomas,V.J.
  TITLE     Hyaluronan Modulates the Biomechanical Properties of the Cornea
  JOURNAL   Invest Ophthalmol Vis Sci 63 (13), 6 (2022)
   PUBMED   36478198
REFERENCE   4  (bases 1 to 5855)
  AUTHORS   Wang,Y., Mack,J.A., Hascall,V.C. and Maytin,E.V.
  TITLE     Transforming Growth Factor-beta Receptor-Mediated, p38
            Mitogen-Activated Protein Kinase-Dependent Signaling Drives
            Enhanced Myofibroblast Differentiation during Skin Wound Healing in
            Mice Lacking Hyaluronan Synthases 1 and 3
  JOURNAL   Am J Pathol 192 (12), 1683-1698 (2022)
   PUBMED   36063901
REFERENCE   5  (bases 1 to 5855)
  AUTHORS   Sun,Y., Ying,X., Li,R., Weng,M., Shi,J. and Chen,Z.
  TITLE     FGF9 Promotes Expression of HAS2 in Palatal Elevation via the
            Wnt/beta-Catenin/TCF7L2 Pathway
  JOURNAL   Biomolecules 12 (11), 1639 (2022)
   PUBMED   36358989
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5855)
  AUTHORS   Spicer,A.P. and McDonald,J.A.
  TITLE     Characterization and molecular evolution of a vertebrate hyaluronan
            synthase gene family
  JOURNAL   J Biol Chem 273 (4), 1923-1932 (1998)
   PUBMED   9442026
REFERENCE   7  (bases 1 to 5855)
  AUTHORS   Weigel,P.H., Hascall,V.C. and Tammi,M.
  TITLE     Hyaluronan synthases
  JOURNAL   J Biol Chem 272 (22), 13997-14000 (1997)
   PUBMED   9206724
  REMARK    Review article
REFERENCE   8  (bases 1 to 5855)
  AUTHORS   Spicer,A.P., Seldin,M.F., Olsen,A.S., Brown,N., Wells,D.E.,
            Doggett,N.A., Itano,N., Kimata,K., Inazawa,J. and McDonald,J.A.
  TITLE     Chromosomal localization of the human and mouse hyaluronan synthase
            genes
  JOURNAL   Genomics 41 (3), 493-497 (1997)
   PUBMED   9169154
REFERENCE   9  (bases 1 to 5855)
  AUTHORS   Spicer,A.P., Olson,J.S. and McDonald,J.A.
  TITLE     Molecular cloning and characterization of a cDNA encoding the third
            putative mammalian hyaluronan synthase
  JOURNAL   J Biol Chem 272 (14), 8957-8961 (1997)
   PUBMED   9083017
REFERENCE   10 (bases 1 to 5855)
  AUTHORS   Hoock,T.C., Peters,L.L. and Lux,S.E.
  TITLE     Isoforms of ankyrin-3 that lack the NH2-terminal repeats associate
            with mouse macrophage lysosomes
  JOURNAL   J Cell Biol 136 (5), 1059-1070 (1997)
   PUBMED   9060470
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK028582.1 and AC123864.4.
            
            On Sep 5, 2016 this sequence version replaced XM_006530699.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028582.1, SRR7652917.842526.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4150              AK028582.1         2-4151
            4151-5855           AC123864.4         125777-127481       c
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.28 cM"
     gene            1..5855
                     /gene="Has3"
                     /note="hyaluronan synthase 3"
                     /db_xref="GeneID:15118"
                     /db_xref="MGI:MGI:109599"
     exon            1..116
                     /gene="Has3"
                     /inference="alignment:Splign:2.1.0"
     CDS             117..1781
                     /gene="Has3"
                     /EC_number="2.4.1.212"
                     /note="HA synthase 3; hyaluronate synthase 3; hyaluronic
                     acid synthase 3"
                     /codon_start=1
                     /product="hyaluronan synthase 3"
                     /protein_id="NP_001317977.1"
                     /db_xref="CCDS:CCDS22640.1"
                     /db_xref="GeneID:15118"
                     /db_xref="MGI:MGI:109599"
                     /translation="MPVQLTTALRVVGTSLFALVVLGGILAAYVTGYQFIHTEKHYLS
                     FGLYGAILGLHLLIQSLFAFLEHRRMRRAGRPLKLHCSQRPRSVALCIAAYQEDPEYL
                     RKCLRSAQRIAFPNLKVVMVVDGNRQEDTYMLDIFHEVLGGTEQAGFFVWRSNFHEAG
                     EGETEASLQEGMERVRAVVWASTFSCIMQKWGGKREVMYTAFKALGNSVDYIQVCDSD
                     TVLDPACTIEMLRVLEEDPQVGGVGGDVQILNKYDSWISFLSSVRYWMAFNVERACQS
                     YFGCVQCISGPLGMYRNSLLQQFLEDWYHQKFLGSKCSFGDDRHLTNRVLSLGYRTKY
                     TARSKCLTETPTRYLRWLNQQTRWSKSYFREWLYNSLWFHKHHLWMTYESVVTGFFPF
                     FLIATVIQLFYRGRIWNILLFLLTVQLVGIIKATYACFLRGNAEMIFMSLYSLLYMSS
                     LLPAKIFAIATINKSGWGTSGRKTIVVNFIGLIPVSIWVAVLLGGLAYTAYCQDLFSE
                     TELAFLVSGAILYGCYWVALLMLYLAIIARRCGKKPEQYSLAFAEV"
     exon            117..755
                     /gene="Has3"
                     /inference="alignment:Splign:2.1.0"
     exon            756..857
                     /gene="Has3"
                     /inference="alignment:Splign:2.1.0"
     exon            858..5855
                     /gene="Has3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5821..5826
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Has3"
                     /note="hexamer: AATAAA"
     polyA_site      5839
                     /gene="Has3"
                     /note="major polyA site"
ORIGIN      
        1 gtcagtcgga gccccagcgg caccgtggag gcagtgcgcg gcggcgggag ccggaggagg
       61 agcatggagc cgccgcgggc ctgctgagca ccggaacgcc ggcagccggc ggcacgatgc
      121 cggtgcagct gactacagcc ctgcgtgtgg tgggcaccag tctgtttgcc ctggtagtgc
      181 tgggaggcat cctggcggcc tatgtgacag gctaccagtt tatccacaca gaaaagcact
      241 acctgtcctt tggcctctac ggtgccatcc tgggtctaca tctgctcatc cagagcctgt
      301 ttgccttcct ggagcaccgt cgaatgcgca gggcagggcg ccccctcaag ctgcactgct
      361 cccagaggcc gcgttcagtg gcactctgca ttgctgccta ccaagaggac cccgaatacc
      421 tgcgcaagtg ccttcgctca gctcagcgca ttgcctttcc aaacctcaag gtggtcatgg
      481 tagtggatgg caatcgccag gaagatacct acatgttgga catcttccat gaggtgctgg
      541 gtggcactga gcaagctggc ttctttgtgt ggcgtagcaa tttccatgag gcgggtgaag
      601 gagagacaga ggccagcctg caggaaggca tggagcgtgt gcgagctgtg gtgtgggcca
      661 gcaccttctc atgcatcatg cagaagtggg ggggcaagcg tgaggtcatg tacactgcct
      721 tcaaggccct tggcaactca gtggactaca tccaggtgtg tgactctgac actgtgctgg
      781 acccagcctg caccattgag atgcttcgag tcttggaaga agatccccaa gtaggaggtg
      841 ttggaggaga tgtccaaatc ctcaacaagt atgattcatg gatctccttc ctgagcagtg
      901 tgaggtactg gatggctttc aacgtggagc gggcctgcca gtcctacttt ggctgtgtgc
      961 aatgtattag tgggcctttg ggcatgtacc gcaacagcct ccttcagcag ttcctggagg
     1021 attggtacca tcagaagttc ctaggcagca agtgcagctt tggggatgat cggcacctta
     1081 ccaaccgagt cctgagtctt ggctaccgga ctaagtatac agcacgctct aagtgcctca
     1141 cagagacccc cactaggtac cttcgatggc tcaatcagca aacccgctgg agcaagtctt
     1201 actttcggga atggctctac aattctctgt ggttccataa gcaccacctc tggatgacct
     1261 atgaatcagt ggtcacaggt ttcttcccat tcttcctcat tgctacagtc atacaacttt
     1321 tctaccgtgg ccgcatctgg aacattctcc tcttcctgct aacagtgcag ctggtgggca
     1381 ttatcaaggc tacctatgcc tgcttccttc gaggcaatgc agagatgatc ttcatgtccc
     1441 tctactccct tctctatatg tccagcctct tgccagccaa gatctttgct attgctacca
     1501 tcaacaagtc tggctggggc acttctggca ggaaaaccat tgtcgtgaac ttcattggcc
     1561 taatccccgt gtccatctgg gtggcagttc ttctaggggg gttagcctac acagcttatt
     1621 gccaggacct gttcagtgag accgagctag ccttcctagt ctctggggcc atcctgtatg
     1681 gctgctactg ggtggccctc ctcatgctgt atctggccat tattgcccgg aggtgtggga
     1741 agaagccaga acagtatagc ctggcttttg cggaggtgtg atataacctc caagtaaagt
     1801 gggaaatgtg caatgggtaa gggagggaat ggaaaagatg gggtgggagg gaggagggtg
     1861 tgttgtgttc tagtttcatg gctgaaagga ccgatctgaa acgcaaagaa tagtgactgt
     1921 ggtatggcct gggtagctct gctcagcaga agcatggcat tgggcagatg ggcttgtatg
     1981 tttctaggct gctcatctgc ctgcccttgc acaggtagtg aactctttga aatgatttta
     2041 cttcttcctg ggaactattg agaacccaga agtgtgctaa aataagtgct aaattccatc
     2101 ctatgtcaac ttctggtaca gaaacaagca atgacagctc atagaatata tcctcctagc
     2161 ccagctgaac atgacaagga gttcctgtca agtgttactg tcccataccg gctactgtcc
     2221 catttcccta acctccgatt agccatcaat gcgttaagac tgtgcctgag ataatgaggc
     2281 tgggaagcct gacctttggt caaaaaacag ggtctaagaa atggtgcttt atgtaattaa
     2341 tatattccac tccacaccaa aacatcccag ggatgagcca agtcaccaga agtaaggaat
     2401 gagtctggtg gggattttat ctgttttggg tgcacagtac aaagcaaagt ctgcaatgca
     2461 ccaaacaaaa gccgcccagt tgttctgctg cacatttgtc atcccagcac ttagaagcag
     2521 aaagagatga gagggtcagg gttaggctag actacatgag atcctgactg tctcatacaa
     2581 aaatggtttg acgggaggat caaagattgc gttggtgcta aaaaccatgg atgccttgga
     2641 gtgaatgtgt tccaactgaa aactgcttgg tcttttacaa aattccccaa gttgtcaaga
     2701 gaacttgctg ggcagactgg atgttttagc catctaccgg gaagactcaa ccccagtgga
     2761 ttcagataag aagtctttag ttattctagc tcattcctac ctgaatgcct ctcacatatg
     2821 gctctcatcc tgcttgctat ttcaatctct tcagagccgc gcatgtcgct tagttgttgt
     2881 tttctggtgg ggagagcagc agttacctct tcattttcat gggtaaattc ccatgtgacc
     2941 atggtagaga atagacgctt acaaggcttt caaggctgtt ccctacatgc atggacgtaa
     3001 caacagcctc tggccacact gttcatcgtc caggactggt caggacttcg gcagctggtt
     3061 agttagcatc tgtaactttc tcgacaatca ttcccaaagg aaagctttct ggagttgccc
     3121 aaatgaacct tcagatccat gttggtcatc tttgagagca tccattctca gtggcttctc
     3181 caaggaattc tacggctaga atagtagatg cacctccatt tgctgcttca gtccagaaac
     3241 caaagtagga gctggaaccg gtccaacagt cttcagtcct cttacagccc tcactggctg
     3301 ccgatttcct tgtttctctt gaggaacgag gagaagcttt tacactcttt tgaagtcaag
     3361 accagattgc tttggcattg aagcgtgggc tcgtgactaa gcctttgact ttgtgggcta
     3421 cttacaattt ccctgaacat ttgttctctg agggaccagc taaccctcag aagcaactgg
     3481 gttggagagc agtgtacata ggtgcttcct acaaaagaat gaagtgcact ccaaaccacc
     3541 aatgggccat cccttgtatt tcccttcctc gtatgaacaa aaggggtttt ccaggtgtag
     3601 ctaacagttg ccacagcaca caaacctcaa gttgctagga aaactgctgg ctggtgtgag
     3661 gcccaactca tggtggctga ggagcagcag ccgtttgtga caggcagttg ctctggggcc
     3721 ttctgttaag aagtgaacta tttgagcact acagtggaga caatccagtc aacttcgggg
     3781 ctcagactaa gggcttgttt ttcttcagct tttacttgaa ggttacagga caatggactc
     3841 tggatgaaaa ctggttgtct accgtcagct ctctgccttg cactgtggtc aactctcctc
     3901 agattaaaga tgcaggtaca gtagtgagct tacaacattt gacctcgact ggatttccta
     3961 atttatttta taagcagcaa aactaaactg ggtctacagc tatatccctg tttcttgcaa
     4021 gggaagagcc tttatacaat tagactcatt ttggttttcc tcattgacaa tttcagcctc
     4081 ttttgtatta tttctacaat aatttgtata tattttttcc tgctttttta ttaaataagg
     4141 agggaacttt atttaacatt aagggtggga gggaatcaga aaggaggata atattcatat
     4201 catgctcttg gcacagccac tgagcacaat gcacaggcct cgtgactctt aagcaaagtc
     4261 acagtgtaca ggtgtgcagc tggagctcat cttcttcagc taagctttta tccgcggttc
     4321 taccttcacc ttgcctatga cctgcaaagt ccaggaggtg cccctgttcc tccaggttgc
     4381 cagacaggtc ataggaacac ctcagaaagt gtagaactct accaggaccc ttctcagaat
     4441 gatccttctc tgcttcacaa tctctgccat ctttctcacc attcctacaa gctgtagtca
     4501 gtgcaggtgc cacagccaat gtcccctcaa ccatcccccc aaccccccca gcctttacag
     4561 cttcctctct gccttgtcct tctacaactc tgggcctctt gaaagcaaag cccccccccc
     4621 cccccccgtt gtcattctct ttgctgatgt ccatgttctc actccagcat caactagtgg
     4681 ggattcaagc tgaggaccac ggctctgcct tttccaggac cacctctgca ctctgggtag
     4741 tggtgctgag ctttgcccag cagtatgaaa cctaagtccc tctcttgaat ctaatgctca
     4801 ggaggtcgta tctcatcttc tggcaccacc acctcaagag tgagatactt agaggtgagc
     4861 tgaagccctg ctgaacttgt agttgcagga tgacccagct ctcagccaat tctcattcat
     4921 gaggccactg gtggtcaccc tagttctatg gaaggtgtct agccagttcc tccagttccc
     4981 atcttattta gcttttgctg tgccgaggcc acctgcacaa ggcagctgtt agggcacagc
     5041 tcttggccag cttcttatgc accgggttca cattcaccag cagtgcattc tcctcttctt
     5101 gcaatactcc ttttgtcctt aaaggtgaag gtcagtctct cctttgtatt aatgacatac
     5161 ttctcagtga ggccctacag gtctcaagcg ccatgcatca gtgtgctgat gcctaacttc
     5221 tggtctatct cctccaacag cttagtcctc ttctctgcca agaccttctg tagctcttca
     5281 ttatcccagc tgtggtgttc ccagggctgg ggtcattctc ctctaacttc tgagtcagga
     5341 gtactgtccc tgtggctctt cttgaaaggc catgggagct aaagttggat attacaaacc
     5401 cgtctttctt aatagtgact ttcaagagtt tcaacatcaa tttggctgta actgactgtt
     5461 tcaaagagca tctcctactt tgtagctgcc cagaatactg tgcagtgcca ttagagacct
     5521 gagtttctgt caccccgtag ccccacctgt tgtccactgt aggtgccatt ccggtgctgt
     5581 tttttgtact ccaaatgttg ctgtggcagc ttgatgactc cttaaaacta agttggcctc
     5641 actgggctgc caccaagtcc tgacacaaaa tgcagacaat tttgggtgac aaggcagccg
     5701 ctatcctgtt gctggcaacc atgagcagct gctcctggct ccattgtttt cgttgccctt
     5761 ggagactggc tttttttgtt gtttatcagg tcaagaattt ttttttattg cacttatcaa
     5821 aataaaaatt cttacataac tctggcgtgt ccatt
//
