LOCUS       NM_080636               3146 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus histidyl-tRNA synthetase 2 (Hars2), transcript variant
            1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_080636
VERSION     NM_080636.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Xu,P., Wang,L., Peng,H., Liu,H., Liu,H., Yuan,Q., Lin,Y., Xu,J.,
            Pang,X., Wu,H. and Yang,T.
  TITLE     Disruption of Hars2 in Cochlear Hair Cells Causes Progressive
            Mitochondrial Dysfunction and Hearing Loss in Mice
  JOURNAL   Front Cell Neurosci 15, 804345 (2021)
   PUBMED   34975414
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3146)
  AUTHORS   Pierce,S.B., Chisholm,K.M., Lynch,E.D., Lee,M.K., Walsh,T.,
            Opitz,J.M., Li,W., Klevit,R.E. and King,M.C.
  TITLE     Mutations in mitochondrial histidyl tRNA synthetase HARS2 cause
            ovarian dysgenesis and sensorineural hearing loss of Perrault
            syndrome
  JOURNAL   Proc Natl Acad Sci U S A 108 (16), 6543-6548 (2011)
   PUBMED   21464306
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC027740.11, AK077621.1 and
            AI593507.1.
            
            On Mar 24, 2015 this sequence version replaced NM_080636.1.
            
            Summary: This gene encodes a putative member of the class II family
            of aminoacyl-tRNA synthetases. These enzymes play a critical role
            in protein biosynthesis by charging tRNAs with their cognate amino
            acids. This protein is encoded by the nuclear genome but is likely
            to be imported to the mitochondrion where it is thought to catalyze
            the ligation of histidine to tRNA molecules. Mutations in a similar
            gene in human have been associated with Perrault syndrome 2
            (PRLTS2). [provided by RefSeq, Mar 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC004596.1, AK145003.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC027740.11        239756-239790
            36-339              AK077621.1         1-304
            340-340             AC027740.11        242165-242165
            341-910             AK077621.1         306-875
            911-1599            AK077621.1         877-1565
            1600-1600           AC027740.11        247120-247120
            1601-2445           AK077621.1         1567-2411
            2446-2723           AC027740.11        248420-248697
            2724-3102           AI593507.1         46-424              c
            3103-3103           AC027740.11        249077-249077
            3104-3146           AI593507.1         2-44                c
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 19.46 cM"
     gene            1..3146
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /note="histidyl-tRNA synthetase 2"
                     /db_xref="GeneID:70791"
                     /db_xref="MGI:MGI:1918041"
     exon            1..286
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..1702
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /EC_number="6.1.1.21"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; probable histidyl-tRNA synthetase,
                     mitochondrial; hisRS; probable histidine--tRNA ligase,
                     mitochondrial; histidyl-tRNA synthetase 2, mitochondrial
                     (putative)"
                     /codon_start=1
                     /product="histidine--tRNA ligase, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_542367.1"
                     /db_xref="CCDS:CCDS29165.1"
                     /db_xref="GeneID:70791"
                     /db_xref="MGI:MGI:1918041"
                     /translation="MPHLGPLRRRAWAALLGQLLRPPSTVCTRGCHSQVAKAVLTSEQ
                     LKSHQEKPNFVIKVPKGTRDLSPQQMVVREKILDKIISCFKRHGAKGLDTPAFELKEM
                     LTEKYEDNFGLMYDLKDQGGELLSLRYDLTVPFARYLAMNKLKKMKRYQVGKVWRRES
                     PAIAQGRYREFCQCDFDIAGEFDPMIPDAECLRIMCEILSGLQLGDFLIKVNDRRVVD
                     GIFAVCGVPESKLRTICSSMDKLDKMSWEGVRHEMVAKKGLAPEVADRIGDFVQYHGG
                     ISLVEDLFKDPRLSQSQLALQGLGDLKLLFEYLRLFGIADKISLDLSLARGLDYYTGV
                     IYEAVLLESPAQAGKETLSVGSVAAGGRYDNLVAQFDPKGHHVPCVGLSIGVERIFYL
                     VEQKMKMSGEKVRTTETQVFVATPQKNFLQERLKIIAELWDAGIKAEMLYKNNPKLLT
                     QLHYCEKADIPLMVIIGEQERNEGVIKLRSVASREEVTINRESLVAEIQKRLSES"
     exon            287..364
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            365..484
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            485..580
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            581..706
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            707..814
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            815..913
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            914..1007
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1135
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1378
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            1379..1495
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1642
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..3135
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2422..2427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /note="hexamer: ATTAAA"
     polyA_site      2446
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /note="major polyA site"
     regulatory      2840..2845
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
                     /note="hexamer: AAGAAA"
     polyA_site      2866
                     /gene="Hars2"
                     /gene_synonym="4631412B19Rik; Harsl; HARSR; HO3"
ORIGIN      
        1 gtctggcgaa ggtggaggga acacttccgg gtcagggagc gtgagatcct cggtgttcaa
       61 tcccaccatc cgaactaact ccgatctcag gtgtttccag ctctcggtag ctttccagaa
      121 atcggactgg cctcgttccc agtcccttga cttcagttcc agaaagccgg cgtcctgcag
      181 cacaatgccc cacctggggc cactgcgtcg cagggcctgg gctgcgctgc tcggccagct
      241 cctgcgacct cccagcactg tgtgcacccg cggctgtcat agccaggttg caaaggcagt
      301 gttaacgtct gaacaactga aatcccatca agagaagccg aattttgtca tcaaggttcc
      361 aaagggcacc agggatttaa gtcctcaaca gatggttgtg agggagaaaa tcctcgataa
      421 gattatcagc tgtttcaaac gtcatggggc gaaggggttg gatacaccag cgtttgagct
      481 aaaagaaatg ctcaccgaga agtatgaaga caacttcggc ctcatgtatg atttgaagga
      541 tcaaggtgga gagttgttgt ctctgcgcta tgaccttact gtcccttttg ctcgatatct
      601 ggccatgaat aaactgaaga aaatgaaacg ataccaagtt ggaaaggtct ggaggcgaga
      661 gagcccagct atagcccagg gccgctaccg ggagttctgc cagtgtgatt ttgacattgc
      721 tggtgagttt gacccaatga tccctgatgc agagtgtttg aggatcatgt gtgaaatcct
      781 aagtggactg cagctggggg actttctcat taaggtaaat gaccgtcggg ttgtagatgg
      841 gatatttgct gtctgtggtg ttcctgagag caagctccga accatctgct cctcaatgga
      901 caaactagac aagatgtctt gggaaggcgt gaggcatgag atggtggcaa agaaaggtct
      961 agctcccgag gtggccgatc gaattgggga cttcgtccaa tatcatgggg ggatatccct
     1021 ggttgaggac ttgttcaagg atcccagact atcccaaagc cagctggcct tgcagggcct
     1081 aggggacctg aagctgctct ttgaatacct gaggttattt ggaattgctg ataagatctc
     1141 ccttgatctg agtctggccc ggggcctgga ctattatact ggagtgattt atgaagcagt
     1201 actgctagag tctccagctc aggctggaaa ggaaactctg agtgtgggta gtgtggctgc
     1261 tggtgggcgc tatgacaatc tggtagccca gtttgatccc aagggccatc acgtgccttg
     1321 tgtggggttg agcattggag tagagagaat cttctacctt gtggaacaga agatgaagat
     1381 gtctggtgag aaggttcgca ccacagagac ccaagtgttt gtggccacac cccagaagaa
     1441 ctttctccaa gaacgtttga agataattgc agagctttgg gatgccggga tcaaggcaga
     1501 gatgctttat aaaaacaacc ctaaactgtt aacccagctg cactattgtg agaaagcaga
     1561 cattcctctg atggtcatta ttggtgagca agagcggaat gaaggtgtca tcaagctccg
     1621 ttccgtggcc agcagagaag aagtgaccat taatcgagaa agtctcgtgg ctgaaattca
     1681 gaagcgactg tctgagtctt gattagtgcc tgatttccat ctgctgcctg tagaagaaaa
     1741 gttttcattt aggaccgagt tctggtggac tttgcaacag tggccagcat ggatgttgta
     1801 caagtgcctt ctgcttccat ctctcctgga tacagaacta cataaggtcc tgaggactcg
     1861 tggactagca ctagcagctg ctctgctaga acgcacatgg ctgtcatgcc tgagaggcat
     1921 cctctagctg caaaggcaga tctgaagtac ctctgaattc tctcaaaggg atgtgaggat
     1981 ggtcgctatg agcaaggctc tggaagagtc gcctgaggct aagactctgc accattgaaa
     2041 tgagaagcta actatgtact ttgttcctga ctgtagcttg ggaactaggt tctggagagc
     2101 ttgtctccat cagactatgg cttgtccatg gagtgttgaa tggaccgaaa aaaagagatg
     2161 gtgtcccagg ggcccagcag agcatctgtt ggttcttcca gttaacagga gaagggtggg
     2221 gctatttcat aattttgaac attgaactta atggatgata agttagcaat atggtctttt
     2281 gaggtttttg aacaccattc cctttgttaa agtaattctc ctggattatt ccatatcctg
     2341 atctggagat tgttctagaa gtggttatat gatatgttac atttcaaaaa tgtactagtg
     2401 gactggagag atggctcacc gattaaaggt gcatattact tttgcagagg acctgagttc
     2461 atttcctagc aaccacatca gggtgattac aactgcctgt aactccagta actggtatct
     2521 gaggctctct tctggcttcc acaaacacct gcacttaatg tgcacatact gatgcacaga
     2581 cacatacaca taattaagaa tgaaataatt ttaaaaagta agcatacttt gagtaattcg
     2641 ttacccaagg attaatttgt gtagacatta ggaaatgttt gaatatatac tggagaactg
     2701 tgtgagttac aagtaatctt tcctggggct cacgtagtag aagaagagaa aagattttct
     2761 tgtttgtcct ctgtcctcta cccacttacc atgacttgtg aagcacacat gtagtcttat
     2821 atacaaaata aattgtaaaa agaaaaacaa cccagtaaaa caccaacata aagtgtggct
     2881 agggaagtgg ctctcttgat agaatgtctg cctagcactt gggaagtgaa agcaggaaga
     2941 tcggaagttc agagtcagct ttggtgccag gcaaggtggc acaggacttt aatcctagca
     3001 caagggaagt agagacaggc caattatctg tgagttatgg gttggccata gctgcatagt
     3061 gaaaccctgt ataaaaattt attcagcctt ggattcacat gagatgctct ctcaataaaa
     3121 caaaacaaaa caaaaaaaaa aaaaaa
//
