LOCUS       NM_177900               3576 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus hyaluronan and proteoglycan link protein 4 (Hapln4),
            mRNA.
ACCESSION   NM_177900
VERSION     NM_177900.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Nojima,K., Miyazaki,H., Hori,T., Vargova,L. and Oohashi,T.
  TITLE     Assessment of Possible Contributions of Hyaluronan and Proteoglycan
            Binding Link Protein 4 to Differential Perineuronal Net Formation
            at the Calyx of Held
  JOURNAL   Front Cell Dev Biol 9, 730550 (2021)
   PUBMED   34604231
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3576)
  AUTHORS   Sucha,P., Chmelova,M., Kamenicka,M., Bochin,M., Oohashi,T. and
            Vargova,L.
  TITLE     The Effect of Hapln4 Link Protein Deficiency on Extracellular Space
            Diffusion Parameters and Perineuronal Nets in the Auditory System
            During Aging
  JOURNAL   Neurochem Res 45 (1), 68-82 (2020)
   PUBMED   31664654
  REMARK    GeneRIF: The Effect of Hapln4 Link Protein Deficiency on
            Extracellular Space Diffusion Parameters and Perineuronal Nets in
            the Auditory System During Aging.
REFERENCE   4  (bases 1 to 3576)
  AUTHORS   Edamatsu,M., Miyano,R., Fujikawa,A., Fujii,F., Hori,T., Sakaba,T.
            and Oohashi,T.
  TITLE     Hapln4/Bral2 is a selective regulator for formation and
            transmission of GABAergic synapses between Purkinje and deep
            cerebellar nuclei neurons
  JOURNAL   J Neurochem 147 (6), 748-763 (2018)
   PUBMED   30125937
REFERENCE   5  (bases 1 to 3576)
  AUTHORS   Popelar,J., Diaz Gomez,M., Lindovsky,J., Rybalko,N., Burianova,J.,
            Oohashi,T. and Syka,J.
  TITLE     The absence of brain-specific link protein Bral2 in perineuronal
            nets hampers auditory temporal resolution and neural adaptation in
            mice
  JOURNAL   Physiol Res 66 (5), 867-880 (2017)
   PUBMED   29020454
  REMARK    GeneRIF: The efficiency of the pre-pulse inhibition of the acoustic
            startle reflex significantly increased in post-natal wild-type mice
            compared to Bral2 knock-out mice.
REFERENCE   6  (bases 1 to 3576)
  AUTHORS   Bekku,Y., Saito,M., Moser,M., Fuchigami,M., Maehara,A.,
            Nakayama,M., Kusachi,S., Ninomiya,Y. and Oohashi,T.
  TITLE     Bral2 is indispensable for the proper localization of brevican and
            the structural integrity of the perineuronal net in the brainstem
            and cerebellum
  JOURNAL   J Comp Neurol 520 (8), 1721-1736 (2012)
   PUBMED   22121037
  REMARK    GeneRIF: these results imply that Bral2-brevican interaction may
            play a key role in synaptic stabilization and the structural
            integrity of brain perineuronal nets
REFERENCE   7  (bases 1 to 3576)
  AUTHORS   Schilling,K. and Oberdick,J.
  TITLE     The treasury of the commons: making use of public gene expression
            resources to better characterize the molecular diversity of
            inhibitory interneurons in the cerebellar cortex
  JOURNAL   Cerebellum 8 (4), 477-489 (2009)
   PUBMED   19554387
REFERENCE   8  (bases 1 to 3576)
  AUTHORS   Czipri,M., Otto,J.M., Cs-Szabo,G., Kamath,R.V., Vermes,C.,
            Firneisz,G., Kolman,K.J., Watanabe,H., Li,Y., Roughley,P.J.,
            Yamada,Y., Olsen,B.R. and Glant,T.T.
  TITLE     Genetic rescue of chondrodysplasia and the perinatal lethal effect
            of cartilage link protein deficiency
  JOURNAL   J Biol Chem 278 (40), 39214-39223 (2003)
   PUBMED   12732630
REFERENCE   9  (bases 1 to 3576)
  AUTHORS   Bekku,Y., Su,W.D., Hirakawa,S., Fassler,R., Ohtsuka,A., Kang,J.S.,
            Sanders,J., Murakami,T., Ninomiya,Y. and Oohashi,T.
  TITLE     Molecular cloning of Bral2, a novel brain-specific link protein,
            and immunohistochemical colocalization with brevican in
            perineuronal nets
  JOURNAL   Mol Cell Neurosci 24 (1), 148-159 (2003)
   PUBMED   14550776
REFERENCE   10 (bases 1 to 3576)
  AUTHORS   Spicer,A.P., Joo,A. and Bowling,R.A. Jr.
  TITLE     A hyaluronan binding link protein gene family whose members are
            physically linked adjacent to chondroitin sulfate proteoglycan core
            protein genes: the missing links
  JOURNAL   J Biol Chem 278 (23), 21083-21091 (2003)
   PUBMED   12663660
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC167132.12.
            
            On Nov 17, 2006 this sequence version replaced NM_177900.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB107882.1, AK034300.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                AC167132.12        143604-143638
            36-156              AC167132.12        144495-144615
            157-519             AC167132.12        144857-145219
            520-843             AC167132.12        146883-147206
            844-3576            AC167132.12        148205-150937
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.15 cM"
     gene            1..3576
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /note="hyaluronan and proteoglycan link protein 4"
                     /db_xref="GeneID:330790"
                     /db_xref="MGI:MGI:2679531"
     exon            1..35
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..1235
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /note="brain linked protein 2; link protein 4; brain link
                     protein 2"
                     /codon_start=1
                     /product="hyaluronan and proteoglycan link protein 4
                     precursor"
                     /protein_id="NP_808568.1"
                     /db_xref="CCDS:CCDS40365.1"
                     /db_xref="GeneID:330790"
                     /db_xref="MGI:MGI:2679531"
                     /translation="MACAPGALGHRALWAVAWGLLLLVPVLAGAQRGRKKVVHVLEGE
                     SGSVVVQTAPGQVVSHRGGTIVLPCRYHYEAAAHGHDGVRLKWTKVVDPLAFADVFVA
                     LGPQHRAFGPYRGRAELQNDGPGDASLVLRNVTLQDYGRYECEVTNELEDDVGMVKLD
                     LEGVVFPYHPRGGRYKMTFVEAQRACAEQDGILASAEQLHAAWRDGLDWCNAGWLRDG
                     SVQYPVSHAREPCGGTGSTGAGGGTNGGVRNYGYRHNAEERYDAFCFTSNLPGRVFFL
                     KPLRPVALAGAVRACAARGATVAKVGQLFAAWKLQLLDRCTAGWLADGSARYPIVNPR
                     TRCGGPRPGVRSLGFPDASRRLFGVYCYRAPGAPDPAPGGWGWGWAGGGGWAGGSRDP
                     AAWTPLRV"
     exon            36..156
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /inference="alignment:Splign:2.1.0"
     exon            157..519
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /inference="alignment:Splign:2.1.0"
     exon            520..843
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /inference="alignment:Splign:2.1.0"
     exon            844..3576
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3182..3187
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /note="hexamer: AATAAA"
     polyA_site      3214
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /note="major polyA site"
     regulatory      3557..3562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
                     /note="hexamer: ATTAAA"
     polyA_site      3576
                     /gene="Hapln4"
                     /gene_synonym="9330174O11; Bral2; Lpr4"
ORIGIN      
        1 gcactggccg ctcagcaagg gccacgacca ggatggcgtg tgctccagga gccctcggtc
       61 accgcgcact ctgggccgtg gcctggggac tcctgctgct ggtccccgtg ctcgcagggg
      121 cgcagcgtgg caggaagaaa gtcgtgcacg tgctggaggg cgagtccggt tccgtggtgg
      181 tgcagacggc gccggggcag gtggtgagcc accgcggtgg taccattgtc ttaccctgcc
      241 gttaccacta cgaggcggcc gcccacggtc acgacggcgt ccgcctcaag tggacgaaag
      301 tggtagaccc tttggctttc gccgatgtct tcgtggctct gggtccccag catcgagcct
      361 tcggtcccta ccgagggcgg gccgaactgc agaatgacgg acctggggat gcctccctgg
      421 tcctccgaaa tgtcaccctt caggactacg ggcgctacga gtgcgaggtc accaatgagc
      481 tggaggatga cgtcggcatg gtcaagctgg atctggaagg ggtggtcttc ccctatcacc
      541 cgcgcggagg tcgctacaag atgactttcg tggaagcgca gcgtgcttgt gccgaacagg
      601 atggcatttt ggcgtccgcg gagcagctgc acgcggcctg gcgcgatggc ctggactggt
      661 gcaacgcggg atggttacgc gatggctcgg tgcagtaccc agtgagccat gcccgggagc
      721 cctgtggcgg gactgggagc actggagctg gtggcggcac caacgggggc gtgcgcaact
      781 atggatatcg tcataacgct gaggaacgct acgatgcctt ctgcttcacg tccaacctcc
      841 cgggccgcgt gtttttcctg aaaccgctgc gacctgtggc cttggccggg gcagtgcgcg
      901 cgtgcgctgc gcggggtgcc acagtggcca aggtcgggca gctgttcgct gcgtggaagc
      961 tgcagctgtt ggaccgctgc actgcaggct ggctggcgga tggcagcgcg cgctatccca
     1021 tcgtgaatcc gcgaactcgc tgtggaggtc ctcggcccgg cgtgcgcagc ctgggtttcc
     1081 ctgacgcctc ccgacgtctc ttcggtgtct actgctaccg cgctccaggt gccccagatc
     1141 cggctccagg cggctggggc tggggctggg ccggcggagg aggttgggct ggagggtcga
     1201 gggatccggc tgcctggacc ccgctgcgtg tatagatctg gaaggatgcg tgtttggggg
     1261 tgttagtggc tgatccagtg gcctcatgca ccccctggat accgatcacg tctcagagtc
     1321 tcagagattc cttggacata tgacacaccc ctgcggtgca ctgcagactg atctagctac
     1381 ctgtggtctt tggacattga ccatacctaa ggtctcctgg ataagcgacc tacccctgcg
     1441 gtgagctgca ggctgaccca cttgttgtgg ttcttggtcg ctgactatgt tgccctctga
     1501 ggatcccctt tggatagtgg atatttcatc ttttagactt tgcccagaag agtcatactc
     1561 tccctaatcc ccgggacccc agtagaccag caggactcta cggtttccaa aagatgaacc
     1621 ttctatggcc ttctgaaaaa caggtggaca cccccctcag tcacctggag gctggacccc
     1681 caggattaac tggagacctg cccccaaacc ttctgtattt cccgggagct ggaaaactgg
     1741 tcttggatcg tcagctgtac actgactcca tcacctggtg aagaaacaga aaaccacagg
     1801 tcacaagatg atcgtgccct gtacgagcaa gtgacccaga ttcccactct ttcagagagt
     1861 ctctttacgt tttcaaggta gcatactttg tccctgggtc aatatggagg acagacccaa
     1921 cacctctggt gtcacaggag agtctcctca gctggctcct ggaacagcgg ccatgcctat
     1981 ccctcaggtt ttcctctgac cagagagtag actcgcttga atgtcccgtc cccgggctcc
     2041 ttgggggatg tcaccaaaga gattccctca gacaagaaac ccctaaaaat ctgacttgca
     2101 gagacaagct gctcggcttc ttaaggtcac ataggccacc aatgcagcct taccaggctc
     2161 acccgctaca cccacagaaa tggcacattc tgccttcctc tgtaaccatg gttaccacac
     2221 ttttcctttc ttactggcct aaagcctaac acctaccact tcatttttgt ctgtccctgc
     2281 cctggtggac tgctgtggag acaatcttcc ccactgagta tcctcagggt aacccaacat
     2341 tcagttctcc aggcctctcc atggaaacag ctgccccttt cccaaagagc atcttctgtt
     2401 tctctcagtt gcccaagccc cgctcctaga cagtttgaaa cacccagact gttgccatgg
     2461 agtccaaacc tctggtgtcc aaggtaacag aatacctgtc ccttcaaggc tttcccctcc
     2521 agggtcccat cctgtccaac tgttgccata gagaacgtcg gtgtctccat gacaactgac
     2581 ctgcagcact caggaacttc ttgctatggg tccctctgac tggagctgcc tgctgtgttc
     2641 ctcaggcgcc tgacaaatgt cccttcttca gatgggccta tgcaggactc tgcttctgga
     2701 acagctatct gcaccatcca tttgcatgct aactttactc cacccagcag ctgagcagga
     2761 gccaccactg caaccccctc cagcatttta aatgttcacc ctgcagacta ggaaactgag
     2821 gctgtggtac cgcacaaagg ccgacctttc cttcttggaa atcaaggagg gcagattctc
     2881 cctgaaacca tcaatttcaa cccatttctg aggacccatg tgtgctcaag ggtcaagacc
     2941 agcccaaatg caggagcctc caggtctcag atgctgctgg aaccagcaag tacccttgcc
     3001 cacacacttt cctgcctgcc ccatctatct cggctaattc cacccctgag tctggaagct
     3061 aaggggctgt gggctccagt cacacctttt gttctaaagt gtgcagccaa acacttatcc
     3121 agactcttct ctgggctagg attgttctga ggtccccagc agcagcctgc agcccaagtt
     3181 caataaacct ttgttttttc tggtgaatgt gaaacaaggc tgccagtccc tgtcagtgca
     3241 gtgggcttct cggttcaaaa gtggctcctc tccctttttt cactgctcca ggatcctgtg
     3301 ggatcctctc tgacctcctc atgtgccctg gggcatgtgt gcatacacat gaatcatagt
     3361 aacatgtcat ctaaaaaacg ttaagtagga gctggctgta gaaatgaggt agccaatctg
     3421 tatttttatt tatttgctta tttattttgt tttttttgag acagggtctc actgtttagc
     3481 cttggctgtc atggaacttg ctacatagac caggcttggc cttgagctca gtgatctgcc
     3541 tgccccaagt gctgagatta aaggtgtaag ccacca
//
