LOCUS       NM_022031               2055 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus hyaluronan and proteoglycan link protein 2 (Hapln2),
            mRNA.
ACCESSION   NM_022031
VERSION     NM_022031.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Edamatsu,M., Miyano,R., Fujikawa,A., Fujii,F., Hori,T., Sakaba,T.
            and Oohashi,T.
  TITLE     Hapln4/Bral2 is a selective regulator for formation and
            transmission of GABAergic synapses between Purkinje and deep
            cerebellar nuclei neurons
  JOURNAL   J Neurochem 147 (6), 748-763 (2018)
   PUBMED   30125937
  REMARK    GeneRIF: Hapln4/Bral2 is a perineuronal nets component that
            selectively contributes to formation and transmission of Purkinje
            cells-deep cerebellar nuclei synapses in the cerebellum
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Susuki,K., Chang,K.J., Zollinger,D.R., Liu,Y., Ogawa,Y.,
            Eshed-Eisenbach,Y., Dours-Zimmermann,M.T., Oses-Prieto,J.A.,
            Burlingame,A.L., Seidenbecher,C.I., Zimmermann,D.R., Oohashi,T.,
            Peles,E. and Rasband,M.N.
  TITLE     Three mechanisms assemble central nervous system nodes of Ranvier
  JOURNAL   Neuron 78 (3), 469-482 (2013)
   PUBMED   23664614
REFERENCE   3  (bases 1 to 2055)
  AUTHORS   Bekku,Y., Vargova,L., Goto,Y., Vorisek,I., Dmytrenko,L.,
            Narasaki,M., Ohtsuka,A., Fassler,R., Ninomiya,Y., Sykova,E. and
            Oohashi,T.
  TITLE     Bral1: its role in diffusion barrier formation and conduction
            velocity in the CNS
  JOURNAL   J Neurosci 30 (8), 3113-3123 (2010)
   PUBMED   20181608
  REMARK    GeneRIF: These findings provide an understanding of the mechanisms
            underlying the accumulation of cations due to diffusion barriers
            around the nodes during saltatory conduction, emphasizing the
            importance of the Bral1-based extramilieu for neuronal
            conductivity.
REFERENCE   4  (bases 1 to 2055)
  AUTHORS   Desai,J., Shannon,M.E., Johnson,M.D., Ruff,D.W., Hughes,L.A.,
            Kerley,M.K., Carpenter,D.A., Johnson,D.K., Rinchik,E.M. and
            Culiat,C.T.
  TITLE     Nell1-deficient mice have reduced expression of extracellular
            matrix proteins causing cranial and vertebral defects
  JOURNAL   Hum Mol Genet 15 (8), 1329-1341 (2006)
   PUBMED   16537572
REFERENCE   5  (bases 1 to 2055)
  AUTHORS   Karim,S.A., Johnson,K.J., Griffiths,I.R. and Vouyiouklis,D.A.
  TITLE     A physical map of the genomic region on mouse chromosome 3
            containing the hindshaker (hsh) mutation
  JOURNAL   Genomics 83 (2), 225-230 (2004)
   PUBMED   14706451
REFERENCE   6  (bases 1 to 2055)
  AUTHORS   Bekku,Y., Su,W.D., Hirakawa,S., Fassler,R., Ohtsuka,A., Kang,J.S.,
            Sanders,J., Murakami,T., Ninomiya,Y. and Oohashi,T.
  TITLE     Molecular cloning of Bral2, a novel brain-specific link protein,
            and immunohistochemical colocalization with brevican in
            perineuronal nets
  JOURNAL   Mol Cell Neurosci 24 (1), 148-159 (2003)
   PUBMED   14550776
REFERENCE   7  (bases 1 to 2055)
  AUTHORS   Spicer,A.P., Joo,A. and Bowling,R.A. Jr.
  TITLE     A hyaluronan binding link protein gene family whose members are
            physically linked adjacent to chondroitin sulfate proteoglycan core
            protein genes: the missing links
  JOURNAL   J Biol Chem 278 (23), 21083-21091 (2003)
   PUBMED   12663660
REFERENCE   8  (bases 1 to 2055)
  AUTHORS   Oohashi,T., Hirakawa,S., Bekku,Y., Rauch,U., Zimmermann,D.R.,
            Su,W.D., Ohtsuka,A., Murakami,T. and Ninomiya,Y.
  TITLE     Bral1, a brain-specific link protein, colocalizing with the
            versican V2 isoform at the nodes of Ranvier in developing and adult
            mouse central nervous systems
  JOURNAL   Mol Cell Neurosci 19 (1), 43-57 (2002)
   PUBMED   11817897
  REMARK    GeneRIF: colocalizing with the versican V2 isoform at the nodes of
            Ranvier in developing and adult mouse central nervous systems.
REFERENCE   9  (bases 1 to 2055)
  AUTHORS   Hirakawa,S., Oohashi,T., Su,W.D., Yoshioka,H., Murakami,T.,
            Arata,J. and Ninomiya,Y.
  TITLE     The brain link protein-1 (BRAL1): cDNA cloning, genomic structure,
            and characterization as a novel link protein expressed in adult
            brain
  JOURNAL   Biochem Biophys Res Commun 276 (3), 982-989 (2000)
   PUBMED   11027579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY122070.1, AB049055.1, AK081264.1 and BE651789.1.
            
            On Oct 26, 2007 this sequence version replaced NM_022031.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB049055.1, BC138584.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-185               BY122070.1         2-186
            186-1304            AB049055.1         115-1233
            1305-1993           AK081264.1         1498-2186
            1994-2055           BE651789.1         444-505
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 38.78 cM"
     gene            1..2055
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /note="hyaluronan and proteoglycan link protein 2"
                     /db_xref="GeneID:73940"
                     /db_xref="MGI:MGI:2137300"
     exon            1..127
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     exon            128..183
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     exon            184..325
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     CDS             238..1263
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /note="brain link protein 1"
                     /codon_start=1
                     /product="hyaluronan and proteoglycan link protein 2
                     precursor"
                     /protein_id="NP_071314.1"
                     /db_xref="CCDS:CCDS17463.1"
                     /db_xref="GeneID:73940"
                     /db_xref="MGI:MGI:2137300"
                     /translation="MPSRIPLPAFCCFLLPWAFTSFHKALGNPAPHPGPHYLLPPIHE
                     VIHSRRGATATLPCVLGTSPPSYKVRWSKVEPGELRETLILITNGLHARDYGLLGGRA
                     SLRRGHRLDASLIIKNVRLEDEGRYRCELINGIEDESVALTLRLEGVVFPYQPSRGRY
                     QFNYFEAKRACEEQDGRLATYGQLYQAWTEGLDWCNAGWLLEGSVRYPVLTARAPCGG
                     HGRPGIRSYGPRDRSRDRYDAFCFTSALAGQVFFVPGRLTLSEAHAACRRRGAVVAKV
                     GHLYAAWKFSGLDQCDGGWLADGSVRFPITTPRPRCGGLPDPGVRSFGFPRPQQASYG
                     TYCYAEK"
     exon            326..679
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     exon            680..796
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     exon            797..979
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     exon            980..2055
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1388..1393
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /note="hexamer: AATAAA"
     polyA_site      1428
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /note="major polyA site"
     regulatory      1663..1668
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
                     /note="hexamer: ACTAAA"
     polyA_site      1684
                     /gene="Hapln2"
                     /gene_synonym="4930401E20Rik; Bral1"
ORIGIN      
        1 agaaaagaaa aggacagaca gacagaaagg gtcagggtcc tgaagcagcc agacatcctg
       61 aaaatctgac tcccatcttg acagcctctt ctgaaaggcc atatatagcc tggcacagag
      121 ggcacaggac aggaagctgc tgagcacagc tcgatttgcc tctgaggaga gatcatcttg
      181 cagtactagg gagtgcccac cacctgtgcc ctgcagacgg tgcttgctga caccatcatg
      241 ccaagcagga tccctctccc tgcgttctgc tgcttccttc tcccctgggc cttcaccagc
      301 tttcacaaag ccctggggaa tccggccccc catccgggtc cccactacct cctgcccccc
      361 atccacgagg tcattcactc tcgtcgtggg gccacggcca cactgccctg cgtcctgggc
      421 acctcgcctc ccagctacaa agtacgctgg agcaaagtgg agcccggtga gctgcgggaa
      481 acgctaatcc tcattaccaa cggactgcac gcccgggact acgggctcct gggagggcgc
      541 gccagcttgc ggagagggca tcggctggac gcctccctga tcatcaagaa cgtgcgtctg
      601 gaggatgagg gtcggtaccg ctgcgagctc atcaatggca ttgaggacga gagcgtggca
      661 ctgaccctgc gcctggaggg tgtggtgttt ccgtaccaac ccagccgagg ccgctaccag
      721 ttcaattact tcgaagcgaa gcgggcgtgt gaagagcagg acggacgcct ggccacttac
      781 ggccaactct accaggcgtg gaccgagggg ctggactggt gcaacgccgg ctggttgctc
      841 gagggctccg tgcgttaccc tgtgctcacc gcgcgggctc cgtgcggcgg ccacgggcgg
      901 ccaggcatcc gcagctacgg gccccgcgac cgcagccgcg accgctatga cgccttctgc
      961 ttcacctcgg ccctggcagg ccaggtgttc ttcgtgccgg ggcggctgac gctgtcagaa
     1021 gcccacgcgg cttgccggag gcgcggagcc gtagtagcca aggtcgggca cctctacgcc
     1081 gcctggaagt tctccggcct ggatcaatgc gacggaggct ggctggcgga cggcagcgtt
     1141 cgcttcccca tcacgacgcc gcggccgcgc tgtgggggtc tccctgaccc aggggtgcgc
     1201 agcttcggct tcccccgacc ccagcaggcg tcctacggga cctactgcta tgccgagaag
     1261 taggagtgcg acagagtggc gggtcccctc tggacagact aaggcccagc cctcacttct
     1321 gccttcccca ggccagcgcg gtgcttcggg ctgctgggca aaggggcgtc ccgggcatta
     1381 actgaccaat aaaataacat ggcaccttct ttgttccata agaacaaaca tatattgcat
     1441 ctatggctca gcaatatact gggtttgtga ttagagagca cccacagcac gaaggacaga
     1501 ggagaggaag gaggagagga agtctcagca gacctggcca tctacacggg agaatacagg
     1561 ctgcccttta tgtctaacca gtcactcaaa ggtttcttat tcaaaatttc caggaactcc
     1621 aaacttccag agctgtctgg gccctacaaa acaaatattc aaactaaact catttctaaa
     1681 tgtacttatc acttccaagg tctgttttat ttagtttatt aattttattt atttattttg
     1741 agacaaggcc tctcaatttt tccttgactg tcctggaact tccactgtgg actaggctgg
     1801 ccttgaattc acacacatca gcctccctct acctcctaag ttctgggatc acatctggct
     1861 aaagtctaca atgaaagacg gtaaattccg tgtggactga gaactcagca gctaagctaa
     1921 cttgatgctc tttcagagga cgaggaccca caatcatctg taactccagt tccagggaat
     1981 ccaacaacct cttctgaccc ccacaggcac caggcaggca cactgtgagc ttgtctacta
     2041 taataaatcc acttg
//
