LOCUS       NM_013500               5055 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus hyaluronan and proteoglycan link protein 1 (Hapln1),
            mRNA.
ACCESSION   NM_013500
VERSION     NM_013500.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Mead,T.J., Bhutada,S., Foulcer,S.J., Peruzzi,N., Nelson,C.M.,
            Seifert,D.E., Larkin,J., Tran-Lundmark,K., Filmus,J. and Apte,S.S.
  TITLE     Combined genetic-pharmacologic inactivation of tightly linked
            ADAMTS proteases in temporally specific windows uncovers distinct
            roles for versican proteolysis and glypican-6 in cardiac
            development
  JOURNAL   Matrix Biol 131, 1-16 (2024)
   PUBMED   38750698
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Sanchez-Ventura,J., Lago,N., Penas,C., Navarro,X. and Udina,E.
  TITLE     Link Protein 1 Is Involved in the Activity-Dependent Modulation of
            Perineuronal Nets in the Spinal Cord
  JOURNAL   Int J Mol Sci 25 (8), 4267 (2024)
   PUBMED   38673852
  REMARK    GeneRIF: Link Protein 1 Is Involved in the Activity-Dependent
            Modulation of Perineuronal Nets in the Spinal Cord.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5055)
  AUTHORS   Marino-Bravante,G.E., Carey,A.E., Huser,L., Dixit,A., Wang,V.,
            Kaur,A., Liu,Y., Ding,S., Schnellmann,R., Gerecht,S., Gu,L.,
            Eisinger-Mathason,T.S.K., Chhabra,Y. and Weeraratna,A.T.
  TITLE     Age-dependent loss of HAPLN1 erodes vascular integrity via indirect
            upregulation of endothelial ICAM1 in melanoma
  JOURNAL   Nat Aging 4 (3), 350-363 (2024)
   PUBMED   38472454
  REMARK    GeneRIF: Age-dependent loss of HAPLN1 erodes vascular integrity via
            indirect upregulation of endothelial ICAM1 in melanoma.
REFERENCE   4  (bases 1 to 5055)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 5055)
  AUTHORS   Chen,X., Song,Q.L., Wang,J.Y., Ji,R., Cao,M.L., Guo,D.Y., Zhang,Y.
            and Yang,J.
  TITLE     FKBP5 regulates trophoblast-macrophage crosstalk in recurrent
            spontaneous abortion through PI3K/AKT and NF-kappaB signaling
            pathways
  JOURNAL   Free Radic Biol Med 209 (Pt 1), 55-69 (2023)
   PUBMED   37827456
REFERENCE   6  (bases 1 to 5055)
  AUTHORS   Kozak,C.A., Su,Y., Raj,N.B. and Pitha,P.M.
  TITLE     Identification and genetic mapping of differentially expressed
            genes in mice differing at the If1 interferon regulatory locus
  JOURNAL   Mamm Genome 10 (9), 853-857 (1999)
   PUBMED   10441734
REFERENCE   7  (bases 1 to 5055)
  AUTHORS   Watanabe,H. and Yamada,Y.
  TITLE     Mice lacking link protein develop dwarfism and craniofacial
            abnormalities
  JOURNAL   Nat Genet 21 (2), 225-229 (1999)
   PUBMED   9988279
REFERENCE   8  (bases 1 to 5055)
  AUTHORS   Deak,F., Mates,L., Krysan,K., Liu,Z., Szabo,P.E., Mann,J.R.,
            Beier,D.R. and Kiss,I.
  TITLE     Characterization and chromosome location of the mouse link protein
            gene (Crtl1)
  JOURNAL   Cytogenet Cell Genet 87 (1-2), 75-79 (1999)
   PUBMED   10640815
REFERENCE   9  (bases 1 to 5055)
  AUTHORS   Wai,A.W., Ng,L.J., Watanabe,H., Yamada,Y., Tam,P.P. and Cheah,K.S.
  TITLE     Disrupted expression of matrix genes in the growth plate of the
            mouse cartilage matrix deficiency (cmd) mutant
  JOURNAL   Dev Genet 22 (4), 349-358 (1998)
   PUBMED   9664687
REFERENCE   10 (bases 1 to 5055)
  AUTHORS   Osborne-Lawrence,S.L., Sinclair,A.K., Hicks,R.C., Lacey,S.W.,
            Eddy,R.L. Jr., Byers,M.G., Shows,T.B. and Duby,A.D.
  TITLE     Complete amino acid sequence of human cartilage link protein
            (CRTL1) deduced from cDNA clones and chromosomal assignment of the
            gene
  JOURNAL   Genomics 8 (3), 562-567 (1990)
   PUBMED   2286376
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK032750.1 and AC154460.2.
            
            On Dec 8, 2007 this sequence version replaced NM_013500.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC066853.1, AK032750.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849379
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2201              AK032750.1         2-2202
            2202-5055           AC154460.2         63454-66307         c
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 45.5 cM"
     gene            1..5055
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /note="hyaluronan and proteoglycan link protein 1"
                     /db_xref="GeneID:12950"
                     /db_xref="MGI:MGI:1337006"
     exon            1..277
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            278..407
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             302..1372
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /note="cartilage link protein 1; cartilage-link protein;
                     proteoglycan link protein; cartilage-linking protein 1"
                     /codon_start=1
                     /product="hyaluronan and proteoglycan link protein 1
                     precursor"
                     /protein_id="NP_038528.3"
                     /db_xref="CCDS:CCDS26671.1"
                     /db_xref="GeneID:12950"
                     /db_xref="MGI:MGI:1337006"
                     /translation="MRSLLLLVLISVCWADHHLSDSYTPPDQDRVIHIQAENGPRLLV
                     EAEQAKVFSHRGGNVTLPCKFYRDPTAFGSGIHKIRIKWTKLTSDYLREVDVFVSMGY
                     HKKTYGGYQGRVFLKGGSDNDASLVITDLTLEDYGRYKCEVIEGLEDDTAVVALELQG
                     VVFPYFPRLGRYNLNFHEARQACLDQDAVIASFDQLYDAWRGGLDWCNAGWLSDGSVQ
                     YPITKPREPCGGQNTVPGVRNYGFWDKDKSRYDVFCFTSNFNGRFYYLIHPTKLTYDE
                     AVQACLNDGAQIAKVGQIFAAWKLLGYDRCDAGWLADGSVRYPISRPRRRCSPTEAAV
                     RFVGFPDKKHKLYGVYCFRAYN"
     exon            408..779
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            780..1082
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..5055
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2392..2397
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /note="hexamer: AATAAA"
     polyA_site      2412
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
     regulatory      5036..5041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /note="hexamer: AATAAA"
     polyA_site      5054
                     /gene="Hapln1"
                     /gene_synonym="CLP; Crtl1; Crtl1l; LP; LP-1"
                     /note="major polyA site"
ORIGIN      
        1 agaggatctg ggaggagaag tctcctggtg acgctttgcc tttcttctgc tcttggtgag
       61 aagtggctcc tttatcctcg gatcaggacc tctgccatcc aacaccacaa agagacattc
      121 tgcacacacg cgctcggaca cacacaccca tacccacacc cacacccaca ccgcactcgc
      181 ccagagacaa acttaaggtc aggacgagca ctagctcact tcatctccag ctcccagcta
      241 agtgggactc agcttctgaa ctggaattca ggacaagcag agaagagtct gagctacaag
      301 gatgagaagt ctgcttctcc tggtgctgat ttcagtctgt tgggctgacc accacctttc
      361 agacagctac actcctccag atcaagacag agttattcac atccaagcag aaaatggccc
      421 ccgtctactt gtggaagcag aacaagccaa ggtcttctct caccgaggtg gcaacgtgac
      481 actgccatgc aaattttatc gagaccctac agcatttggc tcaggaatcc acaaaatccg
      541 catcaagtgg accaagctaa cttcagatta cctcagggaa gtagatgtct tcgtttccat
      601 gggctatcac aagaagacct atggaggcta tcaaggtcga gtgtttctga agggaggcag
      661 tgataatgat gcctccctgg tcatcacgga tcttaccctg gaggattatg ggagatataa
      721 atgtgaggtg attgaagggc tagaagatga tactgctgtg gtggcattgg agttacaagg
      781 tgtggtgttc ccttactttc cacgactggg acgctacaat cttaactttc acgaggcacg
      841 ccaggcttgt ctggaccagg acgcagtgat tgcttccttt gaccagctgt acgatgcctg
      901 gcggggtggg ctggactggt gcaatgctgg ctggctcagt gatggatctg tgcagtaccc
      961 aatcaccaaa ccacgagagc cctgcggggg ccaaaacacg gtgcctggag tcaggaacta
     1021 cgggttttgg gacaaggata aaagcagata tgacgttttc tgttttacat ccaacttcaa
     1081 cggccgattt tactacctga tccaccccac caaactcacc tacgatgagg cggtgcaagc
     1141 ttgtctcaat gacggtgctc agatcgcgaa agtgggccag atatttgctg cctggaagct
     1201 tctgggctat gaccgctgcg atgccggctg gctagcggat ggcagcgtcc gctatcctat
     1261 ttctcgacca agaaggcgct gcagtccgac tgaggctgca gtgcgctttg taggtttccc
     1321 agataaaaag cataagctat acggggtcta ttgcttcaga gcatacaact gagtgtgccc
     1381 taggaagagt cagttcttga gttacgtgaa agttttgttt gtttgtttgt ttttgttgtt
     1441 gttgttttta atatgaactc atgcaagtta ccaaaactgt gataaccctt tttcacttac
     1501 tgtaaagagt cgttttcata aaggccaatt cattcatttg ttttgtaaag ctatcagtcg
     1561 atatatatat tataaagtaa tgtaagcgga aggcattcta aaataggggg gtggcggggg
     1621 gtgctttaga gcctaaactg gttcatgcta catcatccca acaagatgcc atttcatgaa
     1681 tgggatatgt tgtaggctga gaatcaccag gattgtagta agggaagtaa tgctacttgc
     1741 cgcagtaatt tccacaagca caaattttac atatttctac aattttgaaa tgcacttcgg
     1801 taaacaaatt agaacaacaa atttgaaata caggcttctt tacataaacc aggattctgg
     1861 gaggtgcaaa aactcactat cataagggaa caacagagac tttttctaaa agttaatact
     1921 ttaagtctcc agtatacaga atagtttact ctttacaatt ctaccttttt gaccaaaaaa
     1981 ttcatataga ctcagatgca gaataataaa gccacagtta gaactcctag gtcaactcat
     2041 ttatatgaaa tatctactta agtacacaga taatacttta gcaatgaaag agtcctacct
     2101 tctaattaca attacctatc tgagaagact tgttttatta tttttgagta gctttactga
     2161 atcatattta aattctaaga acctttgcta agcagtattt agaatctact cagggcagtt
     2221 aaaacgacaa actgctggac agaaaatgtg taaaatgtag cagcttgatt ttatgttagc
     2281 ttatgaaaca ttattgtcct ataaaaataa ttttaatcta tttaatattt cttatttaca
     2341 ttacatgaca aaaaccatca aatgaatgaa taactctaca cttgcctaag aaataaagac
     2401 tcaacttcca aagttgttct tgtttgtatc cacaatacag tgcccaaata agacctttat
     2461 ttatagaaga aagggaaaaa tacatttcta aatagtctct caagtaggaa atacagtcac
     2521 tgtcagtttc ctttctggtt agaatttggc aaaaataaaa tactggcatt ctcatagcag
     2581 actttacacc tccaggtttt aagtgaaaag ttaagcccag gttcagaaaa gtgactttca
     2641 gtattagcaa ctccagtctt aaattaaagt ttcttgttat gattgcagag accccagagg
     2701 gaccaatatt tatattcaag taatatttct ggtttcccat tatttcacag tgaattcttg
     2761 tgagtaggcc ttagcataat aatccaagta caacatggct agtatgctgt catcaatgtt
     2821 tttatgtaga tttcccccca tgaacataac ataaataaat ctgtttcctg aattgaccat
     2881 ggttgcattt aaagctctgt aataactata ataaaaaatg ctctataaat atagagttat
     2941 gtgtgtggaa agtatagagc agatggaagt ccatgaaacc ataactatca tgatatatta
     3001 cctagataaa atattagaag taaaaataga acctttcttt caggtactta agccatgaca
     3061 atgcatgaag ttataagtta ggatatgcaa attacacaaa agtatacatc caaagaaaat
     3121 gtggggtttc actcaaacag tgctgttgta aattgaattc agacttctaa gctaaacaca
     3181 aattaaagag aatgaatggt ctataatata atgactgtat tacttaagga ggagactcca
     3241 aactagcccc tcaaacacct tacaggctag gctaatctct ttggaggaag atcatacttg
     3301 aacaatcatt acgtatattg taagaatttt gtctgcaatg caagtagata aattttatac
     3361 cgaattaggt gcactccatc cagttattgt catagaactc cacattacag tgcagtagca
     3421 tttcttaact tttgaatact tagtatagga aatggtgctg atcaaaatct ccaaagtgac
     3481 tgttagctga catatgtcta taccaattgt gcaccatttg tccattcttg acaacatcct
     3541 agaatgaaca ctgctgggca gagtttttaa cttctaattt cacacaaagg accagaatcg
     3601 tcatgccctc cctttgaact cctaattgaa aggggtcttt taatgttccc atcttgctgt
     3661 tttcactcca ccaaataatc tattttccta cttcatatct cattataaat gtcttcaaaa
     3721 gaaaaaaagt tgtcttagca caattcctag cagaaagttg acataacact gtctaattca
     3781 gaaatccctt tcgagacttc aagattacca gagtatcaag gtgatgagag gacagacttt
     3841 aaaaaaatga aaacatttta tagtatggcc cacatataaa gaacataata aatctataaa
     3901 ttttactgaa atgccttgat ttgaaacctc taagcaaaaa gaaccaatat atttaaaaat
     3961 acatataagg atgaagataa tttgtcatat taacactgga agccatcttt taatgactgc
     4021 tgccatattt tgtcacagca acttttagcc aaatatagta tttgtaaatt taaattttct
     4081 ttcatataaa gagggccggt acctgtcaaa gtgctatttt atgcagtaag atggtcgctt
     4141 gttattttgg caattagtag actcaaataa gataccaatt tggttgctgg cagccatact
     4201 ggatttcttg atttcagtac tcctaaaagc atgctggtgt catggagtag ctgaccacta
     4261 aaaaagggag gattcaaagg gaatcagttg tcaggtttgg tttagtctta gttttttttt
     4321 ttgttgttgt tttgttttgc tttgtttttt gctttttttt tccatttagt ttgtttcatt
     4381 ttggttttgc atagaatgct tagagaaaac actgacaacc tttttgagaa gtatgtcatt
     4441 gctcaagact gccagcacag tgtacagcaa aagctatgaa taatacagac atcagtaagc
     4501 cacccaatta gaagaaggtc aatatttaaa tgcatttaat agctactaag tacaagtttg
     4561 aggagaaaca atgtgtatta caaaaaagaa tgttttcatt ttctgtaaga tacaatgaac
     4621 tgatgaacaa atattaaaag tcaattttgc tttactatag tatcatttga ttcttatgac
     4681 ttgtagaatc agtcattttt tccccttggg tcaaacttga caaatacacc atgcattctt
     4741 ctgttgctag caatgctttg aatgaatacc cacccaaatg agttagagct ttgacattca
     4801 aacaagaaag tatacttcag aaatttccct taattatttg gaataaatta aacaaccttg
     4861 aagaaaaaaa aaaaactccc aatagtgaga tcaattaaat tttgttttag ttgtaaactc
     4921 tttcaaaatg agggatatca tattcatttt gtcatcattt ttcatatatt tttattcttg
     4981 tcatacaaag tctatgaggc agggtgaaag attctgtgga ggttcttagt ccccaaataa
     5041 aagtaatgaa accat
//
