LOCUS       NM_178878               2716 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus hydroxyacyl-CoA dehydrogenase trifunctional
            multienzyme complex subunit alpha (Hadha), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_178878
VERSION     NM_178878.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Wang,X., Sun,L., Yang,B., Li,W., Zhang,C., Yang,X., Sun,Y.,
            Shen,X., Gao,Y., Ju,B., Gao,Y., Liu,D., Song,J., Jia,X., Su,Y.,
            Jiao,A., Liu,H., Zhang,L., He,L., Lei,L., Chen,W. and Zhang,B.
  TITLE     Zfp335 establishes eTreg lineage and neonatal immune tolerance by
            targeting Hadha-mediated fatty acid oxidation
  JOURNAL   J Clin Invest 133 (20), e166628 (2023)
   PUBMED   37843279
  REMARK    GeneRIF: Zfp335 establishes eTreg lineage and neonatal immune
            tolerance by targeting Hadha-mediated fatty acid oxidation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Paredes,A., Justo-Mendez,R., Jimenez-Blasco,D., Nunez,V.,
            Calero,I., Villalba-Orero,M., Alegre-Marti,A., Fischer,T.,
            Gradillas,A., Sant'Anna,V.A.R., Were,F., Huang,Z.,
            Hernansanz-Agustin,P., Contreras,C., Martinez,F., Camafeita,E.,
            Vazquez,J., Ruiz-Cabello,J., Area-Gomez,E., Sanchez-Cabo,F.,
            Treuter,E., Bolanos,J.P., Estebanez-Perpina,E., Ruperez,F.J.,
            Barbas,C., Enriquez,J.A. and Ricote,M.
  TITLE     gamma-Linolenic acid in maternal milk drives cardiac metabolic
            maturation
  JOURNAL   Nature 618 (7964), 365-373 (2023)
   PUBMED   37225978
  REMARK    Erratum:[Nature. 2023 Jul;619(7968):E24. doi:
            10.1038/s41586-023-06316-w.. PMID: 37322272]
REFERENCE   3  (bases 1 to 2716)
  AUTHORS   Zhang,Y., Lv,Y., Zhang,Q., Wang,X., Han,Q., Liang,Y., He,S.,
            Yuan,Q., Zheng,J., Xu,C., Zhang,X., Wang,Z., Yu,H., Xue,L.,
            Wang,J., Xu,F., Pang,J. and Chen,Y.
  TITLE     ALDH2 attenuates myocardial pyroptosis through breaking down
            Mitochondrion-NLRP3 inflammasome pathway in septic shock
  JOURNAL   Front Pharmacol 14, 1125866 (2023)
   PUBMED   36992838
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2716)
  AUTHORS   Ding,J., Wu,L., Zhu,G., Zhu,J., Luo,P. and Li,Y.
  TITLE     HADHA alleviates hepatic steatosis and oxidative stress in NAFLD
            via inactivation of the MKK3/MAPK pathway
  JOURNAL   Mol Biol Rep 50 (2), 961-970 (2023)
   PUBMED   36376538
  REMARK    GeneRIF: HADHA alleviates hepatic steatosis and oxidative stress in
            NAFLD via inactivation of the MKK3/MAPK pathway.
REFERENCE   5  (bases 1 to 2716)
  AUTHORS   Ma,M., Zhang,C., Cao,R., Tang,D., Sang,X., Zou,S., Wang,X., Xu,H.,
            Liu,G., Dai,L., Tian,Y., Gao,X. and Fu,X.
  TITLE     UBE2O promotes lipid metabolic reprogramming and liver cancer
            progression by mediating HADHA ubiquitination
  JOURNAL   Oncogene 41 (48), 5199-5213 (2022)
   PUBMED   36273042
  REMARK    GeneRIF: UBE2O promotes lipid metabolic reprogramming and liver
            cancer progression by mediating HADHA ubiquitination.
REFERENCE   6  (bases 1 to 2716)
  AUTHORS   Ibdah,J.A., Perlegas,P., Zhao,Y., Angdisen,J., Borgerink,H.,
            Shadoan,M.K., Wagner,J.D., Matern,D., Rinaldo,P. and Cline,J.M.
  TITLE     Mice heterozygous for a defect in mitochondrial trifunctional
            protein develop hepatic steatosis and insulin resistance
  JOURNAL   Gastroenterology 128 (5), 1381-1390 (2005)
   PUBMED   15887119
  REMARK    GeneRIF: Heterozygosity for beta-oxidation defects predisposes to
            fatty live disease and insulin resistance in aging mice
REFERENCE   7  (bases 1 to 2716)
  AUTHORS   Shekhawat,P.S., Yang,H.S., Bennett,M.J., Carter,A.L., Matern,D.,
            Tamai,I. and Ganapathy,V.
  TITLE     Carnitine content and expression of mitochondrial beta-oxidation
            enzymes in placentas of wild-type (OCTN2(+/+)) and OCTN2 Null
            (OCTN2(-/-)) Mice
  JOURNAL   Pediatr Res 56 (3), 323-328 (2004)
   PUBMED   15240869
REFERENCE   8  (bases 1 to 2716)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 2716)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
REFERENCE   10 (bases 1 to 2716)
  AUTHORS   Ibdah,J.A., Paul,H., Zhao,Y., Binford,S., Salleng,K., Cline,M.,
            Matern,D., Bennett,M.J., Rinaldo,P. and Strauss,A.W.
  TITLE     Lack of mitochondrial trifunctional protein in mice causes neonatal
            hypoglycemia and sudden death
  JOURNAL   J Clin Invest 107 (11), 1403-1409 (2001)
   PUBMED   11390422
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK029017.1.
            
            On May 18, 2018 this sequence version replaced NM_178878.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029017.1, AK170683.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2716              AK029017.1         2-2717
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 15.95 cM"
     gene            1..2716
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /note="hydroxyacyl-CoA dehydrogenase trifunctional
                     multienzyme complex subunit alpha"
                     /db_xref="GeneID:97212"
                     /db_xref="MGI:MGI:2135593"
     exon            1..99
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..2324
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /EC_number="4.2.1.17"
                     /EC_number="1.1.1.211"
                     /note="trifunctional enzyme subunit alpha, mitochondrial;
                     monolysocardiolipin acyltransferase; hydroxyacyl-Coenzyme
                     A dehydrogenase/3-ketoacyl-Coenzyme A
                     thiolase/enoyl-Coenzyme A hydratase (trifunctional
                     protein), alpha subunit"
                     /codon_start=1
                     /product="trifunctional enzyme subunit alpha,
                     mitochondrial precursor"
                     /protein_id="NP_849209.1"
                     /db_xref="CCDS:CCDS19155.1"
                     /db_xref="GeneID:97212"
                     /db_xref="MGI:MGI:2135593"
                     /translation="MVASRAIGSLSRFSAFRILRSRGCICRSFTTSSALLTRTHINYG
                     VKGDVAVIRINSPNSKVNTLNKEVQSEFIEVMNEIWANDQIRSAVLISSKPGCFVAGA
                     DINMLSSCTTPQEATRISQEGQRMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRI
                     ATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLV
                     DQLVEPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLVEKLTTYAMTVP
                     FVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKAGLEQGSDAGYLAESQKFGELALTK
                     ESKALMGLYNGQVLCKKNKFGAPQKNVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDT
                     TVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFE
                     DLGVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSKRPEKVIGMHYFSPVDKMQL
                     LEIITTDKTSKDTTASAVAVGLRQGKVIIVVKDGPGFYTTRCLAPMMSEVMRILQEGV
                     DPKKLDALTTGFGFPVGAATLADEVGVDVAQHVAEDLGKAFGERFGGGSVELLKQMVS
                     KGFLGRKSGKGFYIYQEGSKNKSLNSEMDNILANLRLPAKPEVSSDEDVQYRVITRFV
                     NEAVLCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAY
                     GTQFTPCQLLLDHANNSSKKFYQ"
     exon            100..141
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            142..212
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            213..346
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            347..485
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            486..605
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            606..708
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            709..831
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            832..950
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1007
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1117
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1118..1252
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..1424
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1511
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1512..1652
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1653..1721
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1917
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2032
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2033..2178
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     exon            2179..2716
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2686..2691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /note="hexamer: AATAAA"
     polyA_site      2709
                     /gene="Hadha"
                     /gene_synonym="Mtpa; TP-alpha"
                     /note="major polyA site"
ORIGIN      
        1 actagaagag ctttcgtcct cttctgctca agatggtggc gtcccgggcg attggcagtc
       61 tcagtcgctt ctctgccttc aggatcctac gctccagagg ctgcatttgc cgcagcttta
      121 caacgtcttc tgctctgctg accagaaccc atattaatta tggcgtcaaa ggggatgtgg
      181 cagttattcg gattaactca cccaattcaa aggtaaatac attgaataaa gaagtacagt
      241 cagagttcat agaagtcatg aacgaaatct gggccaacga ccaaatcagg agtgctgtcc
      301 ttatctcatc aaagcctggc tgctttgtcg caggggctga catcaacatg ctatcctctt
      361 gtacaacccc ccaagaagca acacgaatat cacaggaagg tcagagaatg tttgagaaac
      421 ttgaaaagtc cccaaagccc gttgtggccg ccatcagtgg atcctgcttg ggaggaggac
      481 ttgagcttgc catagcatgc caatacagaa tagcaacaaa agacagaaaa acagtattag
      541 gtgtccctga agtgttgctg gggatcttac caggagccgg aggtacccag agactgccca
      601 aaatggtggg tgtgcctgct gcatttgaca tgatgctgac tggcaggaac attcgtgcag
      661 acagagcaaa gaaaatggga ctggttgacc agttggtgga accgctggga ccaggaataa
      721 aatcaccaga agaaaggaca attgaatacc tagaggaagt tgcagttaat tttgccaaag
      781 gcctggctga tcggaaagtc tctgcaaagc agagcaaagg ccttgtggaa aagctgacaa
      841 cgtatgccat gactgtccca tttgtcagac aacaggttta caaaacagtt gaagaaaaag
      901 tgaagaagca gactaaaggc ctttatcctg cccctttgaa aataattgat gctgtgaagg
      961 ctggacttga gcaaggcagt gacgctggtt atcttgctga atcgcagaaa tttggagagc
     1021 ttgcattgac caaagaatcg aaggccctga tggggcttta taacggccag gtcctgtgca
     1081 agaaaaataa atttggagca ccacagaaga acgttcagca gctagccatc cttggcgcag
     1141 ggctgatggg ggctggcatt gcccaggtct ctgtggataa gggactgaaa actcttctta
     1201 aagacaccac agtgacgggg ctgggccggg gacagcagca agtgttcaaa gggctgaacg
     1261 acaaagtgaa gaagaaagct ctcacatcat ttgaaaggga ttccatcttc agcaacttga
     1321 ttgggcagct ggattacaaa ggctttgaga aggccgacat ggtgattgag gctgtgtttg
     1381 aggacctcgg tgtaaagcac aaagtgttaa aggaagtgga aagcgtgact ccagaacact
     1441 gtatctttgc cagcaacaca tctgctctcc caatcaatca aattgctgct gtcagcaaaa
     1501 ggcctgagaa ggtgatcggc atgcactact tctcccccgt ggacaagatg cagctcctag
     1561 aaatcatcac cactgacaaa acctccaagg acacaacggc ctctgccgtg gccgtggggc
     1621 tcaggcaagg gaaggtcatc attgtggtca aggacggacc cggcttctac accaccaggt
     1681 gtcttgcacc catgatgtct gaagtcatgc gaatcctcca ggaaggagtt gaccctaaga
     1741 agctggacgc cttgaccaca ggtttcggct tccctgtggg tgctgccacg ctggcagacg
     1801 aagtgggtgt ggatgtagca cagcacgtag cagaagatct aggcaaagcc tttggggagc
     1861 gatttggagg tggcagcgta gaactgctga aacagatggt ctccaagggc ttcttaggtc
     1921 gcaagtctgg gaagggcttt tacatctatc aggagggctc aaagaataag agtttgaatt
     1981 ctgaaatgga taatatcttg gcaaatctga ggctgcctgc caagcccgag gtctcctctg
     2041 atgaagacgt ccaataccgt gtgataacaa ggttcgtgaa cgaggcagtc ctgtgcctac
     2101 aggaagggat cctggccaca ccggcagagg gagacatcgg agctgtcttt gggcttggct
     2161 ttcccccttg tctcggaggg cccttccgct ttgtggatct gtatggtgct cagaaggtag
     2221 tggaccggct acggaagtac gagtctgcct atggaacaca gtttacccca tgccagctgc
     2281 tcctcgacca cgctaacaac tctagcaaga agttctacca gtgacaggcc ctcccaccct
     2341 accccaccat gcactaaccc agacctggca gtgccgcttc tcagctgtgc tgtccaaatt
     2401 atcaggaagc aggaaaaaga ccaaggctag ccttgggctt gctcctccat cagagtgcct
     2461 tctgccctgg cccgctcttc ctcctggtga agtctgactg tgaattaact gtttgcactt
     2521 catgttaagg gggagcccca ctgtgcttct tttgcaagcc ctgcctgaga cccaaatcaa
     2581 cagctaagag taacccagaa cacctgctgc ctgtgccttc ccagaggcca gtggggcctg
     2641 gcagtggtaa gggcattttt tgcaccaagc caaacacagg ataacaataa aatccggact
     2701 ttgtgcctct gccgga
//
