LOCUS       NM_153052                977 bp    mRNA    linear   ROD 17-FEB-2022
DEFINITION  Mus musculus granzyme N (Gzmn), transcript variant 2, mRNA.
ACCESSION   NM_153052
VERSION     NM_153052.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Gallwitz M, Reimer JM and Hellman L.
  TITLE     Expansion of the mast cell chymase locus over the past 200 million
            years of mammalian evolution
  JOURNAL   Immunogenetics 58 (8), 655-669 (2006)
   PUBMED   16807745
REFERENCE   2  (bases 1 to 977)
  AUTHORS   Takano N, Matusi H and Takahashi T.
  TITLE     Granzyme N, a novel granzyme, is expressed in spermatocytes and
            spermatids of the mouse testis
  JOURNAL   Biol Reprod 71 (6), 1785-1795 (2004)
   PUBMED   15269100
  REMARK    GeneRIF: Gzmn is not involved in the process of germ cell apoptosis
            induced by heat shock, but it may be involved in spermatogenesis in
            the mouse testes.
REFERENCE   3  (bases 1 to 977)
  AUTHORS   Grossman WJ, Revell PA, Lu ZH, Johnson H, Bredemeyer AJ and Ley TJ.
  TITLE     The orphan granzymes of humans and mice
  JOURNAL   Curr Opin Immunol 15 (5), 544-552 (2003)
   PUBMED   14499263
  REMARK    Review article
            Erratum:[Curr Opin Immunol. 2003 Dec;15(6):731]
REFERENCE   4  (bases 1 to 977)
  AUTHORS   Sattar R, Ali SA and Abbasi A.
  TITLE     Bioinformatics of granzymes: sequence comparison and structural
            studies on granzyme family by homology modeling
  JOURNAL   Biochem Biophys Res Commun 308 (4), 726-735 (2003)
   PUBMED   12927779
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC091783.9.
            
            On Feb 17, 2022 this sequence version replaced NM_153052.2.
            
            Transcript Variant: This variant (2) uses an alternate splice site
            in the 3' coding region, which results in a frameshift, compared to
            variant 1. It encodes isoform 2, which has a longer and distinct
            C-terminus, compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB049454.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-64                AC091783.9         113684-113747       c
            65-167              AC091783.9         108150-108252       c
            168-318             AC091783.9         106958-107108       c
            319-454             AC091783.9         106378-106513       c
            455-715             AC091783.9         105914-106174       c
            716-977             AC091783.9         104924-105185       c
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 28.19 cM"
     gene            1..977
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /note="granzyme N"
                     /db_xref="GeneID:245839"
                     /db_xref="MGI:MGI:2675494"
     exon            1..64
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /inference="alignment:Splign:2.1.0"
     exon            65..167
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /inference="alignment:Splign:2.1.0"
     CDS             113..859
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2"
                     /codon_start=1
                     /product="granzyme N isoform 2 precursor"
                     /protein_id="NP_694692.1"
                     /db_xref="CCDS:CCDS27145.1"
                     /db_xref="GeneID:245839"
                     /db_xref="MGI:MGI:2675494"
                     /translation="MLPVLILLIFLLPVGDGAEEVIGGHEVKPHSRPYMALVVFLKVN
                     GIGSSCGGFLVQDYFVLTAAHCIGSSMTVTLGAHNLRAQEETQQIIPVNKALPHPDYN
                     PLDHTNDIMLLKLESKAKGTRDVRPLKLPGPKDKVNPGDVCSVAGWGKTSINTTEGSA
                     LLEEAELIIQENKECKKQFRHYSKITEICAGDPNKIEAPSKGDSGGPLVCNNKAHGVL
                     SYVKSKKISSGVFTKVVHFLPWISTNMKLL"
     exon            168..318
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /inference="alignment:Splign:2.1.0"
     exon            319..454
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /inference="alignment:Splign:2.1.0"
     exon            455..715
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /inference="alignment:Splign:2.1.0"
     exon            716..977
                     /gene="Gzmn"
                     /gene_synonym="GrN"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaagaaccct ctaacctctg ttccatggcc tcccatcttg gattccttct tttagccttc
       61 tgatggctgt gtctactccg ctctccttca actgagcagc cttcctggga ggatgctacc
      121 agttctgatt ctcctgatct ttcttctgcc agtaggagat ggagcagagg aggtcatcgg
      181 gggccatgag gtcaagcccc actctcgccc ttacatggca ttagttgtgt ttctgaaagt
      241 taatgggatt ggaagttcct gtggcggctt cctggttcaa gactactttg tgctgacggc
      301 tgctcactgc attggaagct caatgacagt cacactgggg gcccacaacc tccgtgcaca
      361 ggaggagact cagcagatta ttcctgtgaa taaagctctt ccccacccag actataatcc
      421 tctggatcac accaatgaca tcatgctatt aaagctggag agtaaggcca aggggactag
      481 agatgtgagg cccctcaagt tgcctgggcc caaagacaag gtgaatccag gggatgtgtg
      541 cagtgtggct ggctggggga aaacgtccat caataccact gaaggatctg ctctcttaga
      601 agaggctgaa ttgatcatcc aggagaacaa ggaatgcaaa aaacaattcc gtcactattc
      661 caaaatcaca gagatttgtg ctggagaccc aaacaaaata gaggctccgt ccaagggtga
      721 ctcgggggga cccctcgtgt gtaacaacaa agctcatgga gttttgtctt atgtgaaatc
      781 caagaagatc tcttcaggag tcttcactaa ggttgtgcac ttcctgccgt ggataagcac
      841 aaacatgaag ctgctctaac aggagttaaa ccacccgtgc cttaccagcc tgtccgccct
      901 caggcaagaa ccacgtggag tgggcagcaa agaatgaaaa ttcacaataa ataacctcca
      961 gagtgcatag agctgaa
//
