LOCUS       NM_172711               5018 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus GUF1 homolog, GTPase (Guf1), transcript variant 1,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_172711 XM_001052112 XM_001052113 XM_001052114
VERSION     NM_172711.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Gao,Y., Bai,X., Zhang,D., Han,C., Yuan,J., Liu,W., Cao,X., Chen,Z.,
            Shangguan,F., Zhu,Z., Gao,F. and Qin,Y.
  TITLE     Mammalian elongation factor 4 regulates mitochondrial translation
            essential for spermatogenesis
  JOURNAL   Nat Struct Mol Biol 23 (5), 441-449 (2016)
   PUBMED   27065197
  REMARK    GeneRIF: Genetic ablation of mitochondrial EF4 (mtEF4) in mice
            causes testis-specific dysfunction in oxidative phosphorylation,
            leading to male infertility.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK160956.1, AK084627.1, AK032597.1, AK081776.1 and AK028454.1.
            
            On Jul 16, 2015 this sequence version replaced NM_172711.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.125782.1, AK084627.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AK160956.1         2-39
            39-838              AK084627.1         2-801
            839-839             AK032597.1         1829-1829
            840-2256            AK084627.1         803-2219
            2257-3959           AK081776.1         659-2361
            3960-5018           AK028454.1         718-1776
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 37.13 cM"
     gene            1..5018
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /note="GUF1 homolog, GTPase"
                     /db_xref="GeneID:231279"
                     /db_xref="MGI:MGI:2140726"
     exon            1..242
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..2087
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     GTP-binding protein GUF1 homolog; translation factor Guf1,
                     mitochondrial; ribosomal back-translocase; elongation
                     factor 4 homolog; GUF1 GTPase homolog"
                     /codon_start=1
                     /product="translation factor Guf1, mitochondrial isoform
                     1"
                     /protein_id="NP_766299.1"
                     /db_xref="CCDS:CCDS19322.1"
                     /db_xref="GeneID:231279"
                     /db_xref="MGI:MGI:2140726"
                     /translation="MWALVGRALAPWAAGARHAAASEPRAACRLFSAAELKEKPDMSR
                     FPVEDIRNFSIIAHVDHGKSTLADRLLELTGTIDKTKKNKQVLDKLQVERERGITVKA
                     QTASLFYSFGGKQYLLNLIDTPGHVDFSYEVSRSLSACQGVLLVVDANEGIQAQTVAN
                     FFLAFEAQLSVIPVINKIDLKNADPERVGKQIEKVFDIPSEECIKISAKLGTNVDSVL
                     QAVIERIPPPKVHRENPLKALVFDSTFDQYRGVIANIALFDGVVSKGDKIVSAHTKKA
                     YEVNEVGILNPNEQPTHKLYAGQVGFLIAGMKDVTEAQIGDTLYLHNHPVEPLPGFKS
                     AKPMVFAGVYPIDQSEYNNLKSAIEKLTLNDSSVTVHRDSSLALGAGWRLGFLGLLHM
                     EVFNQRLEQEYNASVILTTPTVPYKAVLSSAKLIKEYKEKEITIINPAQFPEKSQVTE
                     YLEPVVLGTVITPTEYTGKIMALCQARRAIQKNMTFIDENRVMLKYLFPLNEIVVDFY
                     DSLKSLSSGYASFDYEDAGYQTAELVKMDILLNGNMVEELVTVVHREKAYTVGKSICE
                     RLKESLPRQLYEIAIQAAVGSKVIARETVKAYRKNVLAKCYGGDITRKMKLLKRQSEG
                     KKKLRKIGNIEIPKDAFIKVLKTQPNK"
     exon            243..354
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            355..503
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            504..584
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            585..662
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            663..746
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            747..811
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            812..1015
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1155
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1279
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1412
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1413..1556
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1557..1690
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1792
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1912
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..1949
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..5018
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2079..2084
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /note="hexamer: AATAAA"
     polyA_site      2097
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /note="major polyA site"
     regulatory      3450..3455
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /note="hexamer: AATACA"
     polyA_site      3470
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
     regulatory      3972..3977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
                     /note="hexamer: AATAAA"
     polyA_site      3997
                     /gene="Guf1"
                     /gene_synonym="4631409J12; EF-4; mtEF4"
ORIGIN      
        1 ataagcggcg cgacctgcag gcggcgcgtt tgcttcgcgt caggcctggg tctcggtgtc
       61 agctcaggcc ggagcgcgac cctggctttg tggtctccgt ggcgggttgg gccctgagcc
      121 gccgccgagg catgtgggcg ctcgtggggc gggctctcgc gccgtgggcc gccggggccc
      181 ggcacgcggc tgcctcggag cccagggccg cctgcaggct cttcagcgcg gccgagctca
      241 aggagaaacc tgacatgtct agatttcctg ttgaagacat tagaaatttc agtatcattg
      301 cacatgtgga tcatggcaaa agcactttag ctgacaggct cctggagcta acaggaacaa
      361 ttgataagac gaagaaaaac aagcaggttc ttgataaatt acaagtagaa cgagaaagag
      421 gaatcactgt gaaggcgcag acagcctctc tgttttacag ctttggaggg aaacagtacc
      481 ttttaaacct catcgacaca ccaggccacg ttgattttag ttatgaagtg tccaggtcac
      541 tgtctgcatg ccaaggtgtt ttactggtag tggatgcaaa tgagggtatt caagctcaaa
      601 ctgtggccaa cttcttcctt gcttttgaag cacagctgtc agtaattcca gttatcaata
      661 agatagatct gaagaatgct gaccctgaaa gggtgggaaa gcagattgaa aaagtatttg
      721 atatccctag tgaggagtgt attaagattt ctgcgaaact tgggacaaac gttgacagtg
      781 tgcttcaggc agtcattgaa cgaatacctc ctccaaaagt ccatcgtgaa aacccactga
      841 aagctttggt atttgactcc acctttgacc agtacagagg ggtgatagcc aatatagcac
      901 tgtttgatgg agtggtttcc aagggagata aaattgtatc tgcacatact aaaaaagcat
      961 atgaagtcaa tgaagtaggc attttgaatc ctaatgaaca gccaactcat aaattatatg
     1021 caggacaggt gggctttctg attgctggga tgaaagatgt cactgaagca caaataggag
     1081 acacattata tttacataat catccagtgg aacccttgcc tgggtttaaa tcagcgaaac
     1141 caatggtgtt tgcaggagtg taccctatag accaatctga gtacaataac ctgaagagtg
     1201 ctatagaaaa actgacctta aatgactcaa gtgtaacagt gcatcgggat agtagtctgg
     1261 ccctgggtgc tggctggagg ttggggtttc ttggactttt acatatggag gttttcaacc
     1321 agcgattaga gcaagaatat aacgcttctg tcattttgac aaccccaact gttccataca
     1381 aagctgttct ttcctctgca aaattaataa aggaatataa agagaaggag atcacaatca
     1441 tcaaccctgc acagttccct gagaagtccc aagtaacaga atatttggaa cctgttgttt
     1501 tgggcactgt tattacaccg actgagtaca ctggaaaaat aatggcgctt tgtcaggcac
     1561 gaagagcaat tcagaaaaat atgactttta ttgatgaaaa tagagttatg cttaaatatc
     1621 tcttcccttt gaatgaaatt gtggtagatt tttatgactc tttgaagtct ctctcttctg
     1681 gatatgcaag ttttgattat gaagatgccg gttaccagac tgcagaactc gttaaaatgg
     1741 atattctgct gaatggaaat atggtggaag agcttgtaac tgtggtccat agagagaaag
     1801 cctacactgt tggcaaaagc atatgtgaac gcctgaagga gtctcttccc agacaactgt
     1861 atgagatagc aattcaagct gctgttggga gcaaagttat tgcaagagaa acagtgaaag
     1921 cctacagaaa aaatgttttg gcaaaatgtt atggtggtga tattacccgg aaaatgaaac
     1981 ttctgaagag acagtccgaa gggaaaaaga agctgaggaa gattggcaac attgagattc
     2041 caaaagacgc tttcataaaa gtcctgaaaa cccagcctaa taaatgactc aagggcagca
     2101 ggtgttctct gttccttctc atttatagtg acagcagaat gaaaagtctg agatgcatat
     2161 tacttccagg gtcttcaact atgacctgct tcagtggttc tcaaccttcc caatgatgcc
     2221 accctttaat atagttcctc atgttgtggt gaccccagac atagaattat tttttgttgc
     2281 tacttcataa cgttaatttt gctactttta tggatcatga tgtaatatac taatgtaaaa
     2341 tgagtatgtc tgatatgcga cctctgtgaa agggttgttg gaccctagtt gagaagtgct
     2401 ggcttgttgg tagagaagag gttaaatgta tgctacattg cttgttctct ctcatgttct
     2461 acgactcact agtaatctcc acagaactcc ttacctaaga ctgagattgt gttactacac
     2521 cgcctcacag gtaactttgg aaattaaagg aggttaatac attttcaata ttcatgaggg
     2581 gcaggcagag tatgttatac atttaatcac agtgaatttt aaacttccct tacacttttt
     2641 gttttttgtt tttagtgtct tgagacagag ttctgccatg tagcatggct agcccagtgt
     2701 cttcagtagg ggttgacacc agtgattgca gcgactctta ggaaggaaag cctttatttc
     2761 tctggggctg gcttattgtt ttaaagggtt atagtccatt attgtcagag tgagaagcat
     2821 tattgcaggt agacatggtg ctggagaagt ggctgggagt ttgtacaggc agcagcagga
     2881 gactgtcaca ccaagcctag cttgagcata ggagacctca aagcttgatt ctatagggac
     2941 acagtgccat tccccctgat agtaccattc cctgtgggcc aagcattcac acatgagtct
     3001 ttggggacca tttctgctca aaccaccaca tacctcaagc ttgtgaccat ctttccacct
     3061 cagccttctc attgttggga ttttacagcc atttgctact gtcttcaact tggtgcgggc
     3121 tttcttacag atgctaaaag gagccctaat tgcatctagt agcaactttc tggaatcacc
     3181 ttgtattaca gtttttagct caaatgcttg atctgatatg acttgctttt taagatatag
     3241 actcaatgtc ttactagaca tttgttgttt ttttatgcct atacattgaa tcaagcattt
     3301 ctgtatcttg ttttttatga gtacattcct gcaaaatgcc ataatttgat attagcagca
     3361 gctgttaata aattagaagc tattaactaa acatacctaa atgacacagc taataaatgg
     3421 attagtttca tgttcataac aaactgaaaa atacattact ccaagaaaaa gtcttgatac
     3481 tggattttat tttctgccgt ctgtcttgag catgacataa atctggtagg aaaaaaggtt
     3541 acagatgagt cttagcattt tggtgggatt ttaaagtgag gatttataag ttaggtagcc
     3601 ttagtttgtc ctattacata aaccatctca cttctcccag cttgatttgc taggtataaa
     3661 ataagttccc actgcagtat ctttttaaaa tggcaatata tttgaaacta agacaatgca
     3721 atttttaaaa tgttgttctt atattattgg taagggaaag ttaaaaaact ggtgtaatct
     3781 ctaccgtgta gtgcttgagc tactttagat atgggctatt ctttaaccca taattcttta
     3841 ggaattaacg ttgtagtaac aggagaaaac atttgctagg ctgaattttg agttatataa
     3901 cttgagctta ttccttcggg ttctagaatc acaatttgta tagtcgtatg acaaaaaaat
     3961 aattgccact taataaattt ggtatgggtt gccagtatgc tgagttaaat acactttttt
     4021 cagtctctgt aacagcagag ggtaagatgc tgccacttat atcccctggt gacagaagct
     4081 aaataatgtt ggttttgctt caaagacaaa gatcattgct acatctgaca tatcttcaat
     4141 ggtttctaag cgtcttaata gtatatcaca gtaagttagg atgctatagt gattagggaa
     4201 gtgctgagga gagacactgt gagacagcat tagggataga atgaaaaaaa gatctttttt
     4261 taagaccttt ggagcagagg gaatgaatgg tacatcagaa gagactgtaa actcttcatt
     4321 atgatgctaa acagccaaag atggggtaat cttgagtaaa cgtgatagtg ttaaaatcat
     4381 tactgcagca tggatgctac tagttgactt gttaactaaa atatgttaag agcataagga
     4441 ccccttgagc aaattatgat tctgccaggt atttgtctaa gcaaaatgta atgatgtagt
     4501 gtgactatat ttctattata ggacgaaaga gaggaagaac accagtaagg ctaacttcta
     4561 ctttgtaccc atctgaatga atgatgtacc agttttaata ctaagtaggg aggcagaata
     4621 gcctaagaga gatttagagc tcagcttaat tttagaatag tccatagtat tcctatgtgg
     4681 tgttcttcag caggctattg gaaatgtgaa tttgaaggtc agaagacaga tcatagtgct
     4741 atgaatccaa agaggttcca gaatatatat ttatgtgctc ggggatgtga aaccctatca
     4801 tgtggggctt ctcagcttcc agtaatttgt atagcacctt tatacttaca tggttgtttg
     4861 atatctccct aatatatata tatatatata tatatgcata tgcattttaa tatttatgca
     4921 ttatatattt atgtatgtat atttggctta gtttcatgaa taacagctat taagacataa
     4981 ctgggaatta aagcaccatt gttggtgaca ttattgtt
//
