LOCUS       NM_001127318            3951 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus guanylate cyclase 2c (Gucy2c), transcript variant 1,
            mRNA.
ACCESSION   NM_001127318
VERSION     NM_001127318.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3951)
  AUTHORS   Barton,J.R., Londregan,A.K., Alexander,T.D., Entezari,A.A.,
            Bar-Ad,S., Cheng,L., Lepore,A.C., Snook,A.E., Covarrubias,M. and
            Waldman,S.A.
  TITLE     Intestinal neuropod cell GUCY2C regulates visceral pain
  JOURNAL   J Clin Invest 133 (4), e165578 (2023)
   PUBMED   36548082
  REMARK    GeneRIF: Intestinal neuropod cell GUCY2C regulates visceral pain.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3951)
  AUTHORS   Ratko,M., Habek,N., Dobrivojevic Radmilovic,M., Skokic,S.,
            Justic,H., Baric,A. and Dugandzic,A.
  TITLE     Role of uroguanylin's signalling pathway in the development of
            ischaemic stroke
  JOURNAL   Eur J Neurosci 56 (1), 3720-3737 (2022)
   PUBMED   35445449
REFERENCE   5  (bases 1 to 3951)
  AUTHORS   Mishra,V., Bose,A., Kiran,S., Banerjee,S., Shah,I.A.,
            Chaukimath,P., Reshi,M.M., Srinivas,S., Barman,A. and
            Visweswariah,S.S.
  TITLE     Gut-associated cGMP mediates colitis and dysbiosis in a mouse model
            of an activating mutation in GUCY2C
  JOURNAL   J Exp Med 218 (11) (2021)
   PUBMED   34546338
  REMARK    GeneRIF: Gut-associated cGMP mediates colitis and dysbiosis in a
            mouse model of an activating mutation in GUCY2C.
            Erratum:[J Exp Med. 2021 Nov 1;218(11):e2021047909292021c. doi:
            10.1084/jem.2021047909292021c.. PMID: 34614150]
REFERENCE   6  (bases 1 to 3951)
  AUTHORS   Isomoto,S., Horio,Y., Matsumoto,S., Kondo,C., Yamada,M.,
            Gilbert,D.J., Copeland,N.G., Jenkins,N.A. and Kurachi,Y.
  TITLE     Sur2 and Kcnj8 genes are tightly linked on the distal region of
            mouse chromosome 6
  JOURNAL   Mamm Genome 8 (10), 790-791 (1997)
   PUBMED   9321483
REFERENCE   7  (bases 1 to 3951)
  AUTHORS   Schulz,S., Lopez,M.J., Kuhn,M. and Garbers,D.L.
  TITLE     Disruption of the guanylyl cyclase-C gene leads to a paradoxical
            phenotype of viable but heat-stable enterotoxin-resistant mice
  JOURNAL   J Clin Invest 100 (6), 1590-1595 (1997)
   PUBMED   9294128
REFERENCE   8  (bases 1 to 3951)
  AUTHORS   Swenson,E.S., Mann,E.A., Jump,M.L., Witte,D.P. and Giannella,R.A.
  TITLE     The guanylin/STa receptor is expressed in crypts and apical
            epithelium throughout the mouse intestine
  JOURNAL   Biochem Biophys Res Commun 225 (3), 1009-1014 (1996)
   PUBMED   8780725
REFERENCE   9  (bases 1 to 3951)
  AUTHORS   Mann,E.A., Swenson,E.S., Copeland,N.G., Gilbert,D.J., Jenkins,N.A.,
            Taguchi,T., Testa,J.R. and Giannella,R.A.
  TITLE     Localization of the guanylyl cyclase C gene to mouse chromosome 6
            and human chromosome 12p12
  JOURNAL   Genomics 34 (2), 265-267 (1996)
   PUBMED   8661067
REFERENCE   10 (bases 1 to 3951)
  AUTHORS   Yang,R.B., Fulle,H.J. and Garbers,D.L.
  TITLE     Chromosomal localization and genomic organization of genes encoding
            guanylyl cyclase receptors expressed in olfactory sensory neurons
            and retina
  JOURNAL   Genomics 31 (3), 367-372 (1996)
   PUBMED   8838319
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK136496.1 and CF353627.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK136496.1, SRR7974084.31361.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3934              AK136496.1         25-3958
            3935-3951           CF353627.1         244-260
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 66.67 cM"
     gene            1..3951
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /note="guanylate cyclase 2c"
                     /db_xref="GeneID:14917"
                     /db_xref="MGI:MGI:106903"
     exon            1..353
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     CDS             137..3355
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /EC_number="4.6.1.2"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; guanylyl cyclase C; heat-stable enterotoxin
                     receptor; intestinal guanylate cyclase; STA receptor"
                     /codon_start=1
                     /product="guanylyl cyclase C isoform 1 precursor"
                     /protein_id="NP_001120790.1"
                     /db_xref="CCDS:CCDS51941.1"
                     /db_xref="GeneID:14917"
                     /db_xref="MGI:MGI:106903"
                     /translation="MTSLLGLAVRLLLFQPALMVFWASQVRQNCRNGSYEISVLMMDN
                     SAYKEPMQNLREAVEEGLDIVRKRLREADLNVTVNATFIYSDGLIHKSGDCRSSTCEG
                     LDLLREITRDHKMGCALMGPSCTYSTFQMYLDTELNYPMISAGSYGLSCDYKETLTRI
                     LPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSE
                     VLNFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLF
                     SNHYFEENTTAPEYMDNVLVLTLPSEQSTSNTSVAERFSSGRSDFSLAYLEGTLLFGH
                     MLQTFLENGENVTGPKFARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYK
                     VLMKYDTHKNKTIPVAENPNFIWKNHKLPNDVPGLGPQILMIAVFTLTGILVVLLLIA
                     LLVLRKYRRDHALRQKKWSHIPSENIFPLETNETNHISLKIDDDRRRDTIQRVRQCKY
                     DKKKVILKDLKHSDGNFSEKQKIDLNKLLQSDYYNLTKFYGTVKLDTRIFGVVEYCER
                     GSLREVLNDTISYPDGTFMDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDS
                     RMVVKITDFGCNSILPPKKDLWTAPEHLRQATISQKGDVYSFAIIAQEIILRKETFYT
                     LSCRDHNEKIFRVENSYGKPFRPDLFLETADEKELEVYLLVKSCWEEDPEKRPDFKKI
                     ESTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADHLNFM
                     LLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFD
                     QIVDHHDVYKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFIGTFELEHLPGL
                     PVWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTITILK
                     RTDCQFLYEVRGETYLKGRGTETTYWLTGMKDQEYNLPSPPTVENQQRLQTEFSDMIV
                     SALQKRQASGKKSRRPTRVASYKKGFLEYMQLNNSDHDSTYF"
     exon            354..466
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            467..531
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            532..747
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            748..869
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            870..966
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1084
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1220
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1306
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1418
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1419..1500
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1501..1606
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1607..1669
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1670..1741
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1846
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1933
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2066
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2204
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2205..2290
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2291..2382
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2383..2541
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2542..2734
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2735..2909
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3008
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            3009..3103
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            3104..3180
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     exon            3181..3936
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3916..3921
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /note="hexamer: AATAAA"
     polyA_site      3936
                     /gene="Gucy2c"
                     /gene_synonym="GC-C; Gcc"
                     /note="major polyA site"
ORIGIN      
        1 gaccagtgtg gcaagaccag aaaggtgcgt ggggaagaga agaccaaggg actctgctag
       61 cgactctcca gaggggctcc ctgtgtctct aaaagcgagc aatccagggg ggcatggtgc
      121 tacggtgagc caggtcatga cgtcactgct gggcttggct gtgcggttac tgctcttcca
      181 gcccgcgctg atggtgttct gggcctctca ggtgaggcag aactgccgca atggcagcta
      241 cgagatcagc gtcctgatga tggacaactc agcctacaaa gaacctatgc aaaacctgag
      301 ggaggctgtg gaggaaggac tggacatagt gcgaaagcgc ctgcgtgaag ccgacctaaa
      361 tgtgactgtg aacgcgactt tcatctactc cgacggtctg attcataagt caggtgactg
      421 ccggagcagc acctgtgaag gccttgacct actcagggag attacaagag atcataagat
      481 gggctgcgcc ctcatggggc cctcgtgcac gtattccacc ttccagatgt acctcgacac
      541 agagttgaac tatcccatga tttccgctgg aagttatgga ttgtcctgtg actataagga
      601 aaccctaacc aggatcctgc ctccagccag gaagctgatg tacttcttgg tcgatttctg
      661 gaaagtcaac aatgcatctt tcaaaccctt ttcctggaac tcttcgtatg tttacaagaa
      721 tggatcggaa cctgaagatt gtttctggta cctcaatgct ctggaggctg gggtgtccta
      781 tttttctgag gtgctcaact tcaaggatgt actgagacgc agcgaacagt tccaggaaat
      841 cttaacaggc cataacagaa agagcaatgt gattgttatg tgtggcacgc cagaaagctt
      901 ctatgatgtg aaaggtgacc tccaagtggc tgaagatact gttgtcatcc tggtagatct
      961 gttcagtaac cattactttg aggagaacac cacagctcct gagtatatgg acaatgtcct
     1021 cgtcctgacg ctgccgtctg aacagtccac ctcaaacacc tctgtcgccg agaggttttc
     1081 atcggggaga agtgactttt ctctcgctta cttggaggga accttgctat ttggacacat
     1141 gctgcagacg tttcttgaaa atggagaaaa tgtcacgggt cccaagtttg ctcgtgcatt
     1201 caggaatctc acttttcaag gctttgcagg acctgtgact ctggatgaca gtggggacat
     1261 tgacaacatt atgtcccttc tgtatgtgtc tctggatacc aggaaataca aggttcttat
     1321 gaagtatgac acccacaaaa acaaaactat tccggtggct gagaacccca acttcatctg
     1381 gaagaaccac aagctcccca atgacgttcc tgggctgggc cctcaaatcc tgatgattgc
     1441 cgtcttcacg ctcacgggga tcctggtagt tctgctgctg attgccctcc tcgtgctgag
     1501 aaaatacaga agagatcatg cacttcgaca gaagaaatgg tcccacattc cttctgaaaa
     1561 catctttcct ctggagacca acgagaccaa ccacatcagc ctgaagattg acgatgacag
     1621 gagacgagac acaatccaga gagtgcgaca gtgcaaatac gacaagaaga aagtgattct
     1681 gaaagacctc aagcacagcg acgggaactt cagtgagaag cagaagatag acctgaacaa
     1741 gttgctgcag tctgactact acaacctgac taagttctac ggcaccgtga agctggacac
     1801 caggatcttt ggggtggttg agtactgcga gaggggatcc ctccgggaag tgttaaacga
     1861 cacaatttcc taccctgacg gcacgttcat ggattgggag tttaagatct ctgtcttaaa
     1921 tgacatcgct aaggggatgt cctacctgca ctccagtaag attgaagtcc acgggcgtct
     1981 caaatccacc aactgcgtgg tggacagccg catggtggtg aagatcaccg actttgggtg
     2041 caattccatc ctgcctccaa aaaaagacct gtggacggcc ccggagcacc tgcgccaggc
     2101 caccatctct cagaaaggag acgtgtacag cttcgccatc attgcccagg agatcatcct
     2161 ccgtaaggag actttttaca cgctgagctg tcgggatcac aatgagaaga ttttcagagt
     2221 ggaaaattca tacgggaaac ctttccgccc agacctcttc ctggagactg cagatgagaa
     2281 ggagctggag gtctatctac ttgtcaaaag ctgttgggag gaggatccag aaaagaggcc
     2341 agatttcaag aaaatcgaga gcacactggc caagatattt ggccttttcc atgaccagaa
     2401 aaacgagtct tacatggaca ccttgatccg acgtctccag ctgtactctc gaaacctgga
     2461 acatctggtg gaggaaagga ctcagctgta caaggcggag agggacaggg ctgaccacct
     2521 taacttcatg ctcctcccac ggctggtggt aaagtcactg aaggagaaag gcatcgtgga
     2581 gccagagctg tacgaagaag tcacaatcta cttcagtgac attgtgggct tcaccaccat
     2641 ctgcaagtat agcacgccca tggaggtggt ggacatgctc aacgacatct acaagagctt
     2701 tgaccagatt gtggaccacc atgacgtcta caaggtagaa accatcggtg acgcctacgt
     2761 ggtggccagc ggtctgccta tgagaaacgg caaccgacac gcggtagaca tttccaagat
     2821 ggccttggac atcctcagct tcatagggac ctttgagttg gagcatctcc ctggcctccc
     2881 cgtgtggatc cgcattggag ttcattctgg gccctgcgct gctggtgttg tggggatcaa
     2941 gatgcctcgc tattgcctgt ttggagacac tgtcaacact gcctccagga tggaatccac
     3001 cggcctcccc ttgaggattc acatgagcag ctccaccata accatcctga agagaacgga
     3061 ttgccagttc ctgtatgaag tgaggggaga aacctactta aagggaagag ggacagagac
     3121 cacatactgg ctgactggga tgaaggacca agaatacaac ctgccatccc caccgacagt
     3181 ggagaaccaa cagcgtctgc agactgagtt ctcagacatg atcgttagcg ccttacagaa
     3241 aagacaggcc tcgggcaaga agagccggag gcccactcgg gtggccagct acaagaaagg
     3301 ctttctggaa tacatgcagc tgaacaattc agaccacgat agcacctatt tttagaccaa
     3361 gtgaggtctg agaactgaca gtagcaacct cctatatcat gaatctgtat tttccagaga
     3421 cctcaacaac atagacaagc acttagcctc agtgccctga ctggaacgta gaaccaaccc
     3481 ctcaagtcat gtgggtctga ttttgggttg gttggttggt tggttggttg gttttggttt
     3541 tgttgagaca gagtttcgtg tatcccaagc tggtctcaaa ctcactgtgt agcagcagat
     3601 gactttggac ttctaagatc atccgtgtgt gttcctgaca gtgtgatgag tgtatacgtc
     3661 agagccctgt cccacagttc tccatggagc atcaacctga atggaggagc gaggaggggg
     3721 aatgtcctgt gctgtacaga acttggggtt ttgttctaat tttatctctg gtttggtttt
     3781 gttttctggc tccttccctc tctatgtgtg agagaagttt ttaaattgtc tgaattgtat
     3841 gctaagtagc ttatctacaa gaaagtgtgt ttaactagtg atttttgcag aaaccatgct
     3901 ggatattagg taaaaaataa aagtgtttag agtctaaaaa aaaaaaaaaa a
//
