LOCUS       NM_017469               3251 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus guanylate cyclase 1, soluble, beta 1 (Gucy1b1),
            transcript variant 1, mRNA.
ACCESSION   NM_017469
VERSION     NM_017469.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   He,H., Yang,W., Su,N., Zhang,C., Dai,J., Han,F., Singhal,M.,
            Bai,W., Zhu,X., Zhu,J., Liu,Z., Xia,W., Liu,X., Zhang,C., Jiang,K.,
            Huang,W., Chen,D., Wang,Z., He,X., Kirchhoff,F., Li,Z., Liu,C.,
            Huan,J., Wang,X., Wei,W., Wang,J., Augustin,H.G. and Hu,J.
  TITLE     Activating NO-sGC crosstalk in the mouse vascular niche promotes
            vascular integrity and mitigates acute lung injury
  JOURNAL   J Exp Med 220 (2) (2023)
   PUBMED   36350314
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Bork,N.I., Kuret,A., Cruz Santos,M., Molina,C.E., Reiter,B.,
            Reichenspurner,H., Friebe,A., Skryabin,B.V., Rozhdestvensky,T.S.,
            Kuhn,M., Lukowski,R. and Nikolaev,V.O.
  TITLE     Rise of cGMP by partial phosphodiesterase-3A degradation enhances
            cardioprotection during hypoxia
  JOURNAL   Redox Biol 48, 102179 (2021)
   PUBMED   34763298
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   3  (bases 1 to 3251)
  AUTHORS   Wang,X., Du,W., Li,M., Zhang,Y., Li,H., Sun,K., Liu,J., Dong,P.,
            Meng,X., Yi,W., Yang,L., Zhao,R. and Hu,J.
  TITLE     The beta subunit of soluble guanylyl cyclase GUCY1B3 exerts
            cardioprotective effects against ischemic injury via the
            PKCepsilon/Akt pathway
  JOURNAL   J Cell Biochem 120 (3), 3071-3081 (2019)
   PUBMED   30485489
  REMARK    GeneRIF: GUCY1B3 was upregulated in neonatal rat ventricular
            myocytes in response to ischemia and reperfusion (IR) injury, and
            restored IR-induced cell death and apoptosis. Other results suggest
            that GUCY1B3 has an antiapoptotic effect and this effect is
            mediated by the activation of PKCepsilon and Akt.
REFERENCE   4  (bases 1 to 3251)
  AUTHORS   Mohammadoo Khorasani,M., Karami Tehrani,F., Parizadeh,S.M.R. and
            Atri,M.
  TITLE     Differential expression of alternative transcripts of soluble
            guanylyl cyclase, GYCY1a3 and GUCY1b3 genes, in the malignant and
            benign breast tumors
  JOURNAL   Nitric Oxide 83, 65-71 (2019)
   PUBMED   30597209
  REMARK    GeneRIF: data have provided some evidence for an alteration in the
            expression of alpha1 and beta1 oluble guanylyl cyclase alternative
            splicing forms which may contribute to the loss of sGC functions in
            breast cancer
REFERENCE   5  (bases 1 to 3251)
  AUTHORS   Wen,L., Feil,S., Wolters,M., Thunemann,M., Regler,F., Schmidt,K.,
            Friebe,A., Olbrich,M., Langer,H., Gawaz,M., de Wit,C. and Feil,R.
  TITLE     A shear-dependent NO-cGMP-cGKI cascade in platelets acts as an
            auto-regulatory brake of thrombosis
  JOURNAL   Nat Commun 9 (1), 4301 (2018)
   PUBMED   30327468
  REMARK    Erratum:[Nat Commun. 2018 Nov 20;9(1):4969. doi:
            10.1038/s41467-018-07409-1.. PMID: 30459325]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3251)
  AUTHORS   Kajiya,K., Huggenberger,R., Drinnenberg,I., Ma,B. and Detmar,M.
  TITLE     Nitric oxide mediates lymphatic vessel activation via soluble
            guanylate cyclase alpha1beta1-impact on inflammation
  JOURNAL   FASEB J 22 (2), 530-537 (2008)
   PUBMED   17855621
  REMARK    GeneRIF: findings identify a crucial role of the
            NO/sGCalpha1beta1/cGMP pathway in modulating lymphatic vessel
            function [soluble guanylate cyclase alpha1beta1]
REFERENCE   7  (bases 1 to 3251)
  AUTHORS   Nimmegeers,S., Sips,P., Buys,E., Brouckaert,P. and Van de Voorde,J.
  TITLE     Functional role of the soluble guanylyl cyclase alpha(1) subunit in
            vascular smooth muscle relaxation
  JOURNAL   Cardiovasc Res 76 (1), 149-159 (2007)
   PUBMED   17610859
REFERENCE   8  (bases 1 to 3251)
  AUTHORS   Friebe,A., Mergia,E., Dangel,O., Lange,A. and Koesling,D.
  TITLE     Fatal gastrointestinal obstruction and hypertension in mice lacking
            nitric oxide-sensitive guanylyl cyclase
  JOURNAL   Proc Natl Acad Sci U S A 104 (18), 7699-7704 (2007)
   PUBMED   17452643
  REMARK    GeneRIF: Knockout of GCbeta1 completely disrupts the NO/cGMP
            signaling cascade and provides evidence for the unique role of
            NO-GC as NO receptor.
REFERENCE   9  (bases 1 to 3251)
  AUTHORS   Fenske,T.S., McMahon,C., Edwin,D., Jarvis,J.C., Cheverud,J.M.,
            Minn,M., Mathews,V., Bogue,M.A., Province,M.A., McLeod,H.L. and
            Graubert,T.A.
  TITLE     Identification of candidate alkylator-induced cancer susceptibility
            genes by whole genome scanning in mice
  JOURNAL   Cancer Res 66 (10), 5029-5038 (2006)
   PUBMED   16707424
REFERENCE   10 (bases 1 to 3251)
  AUTHORS   Sharina,I.G., Krumenacker,J.S., Martin,E. and Murad,F.
  TITLE     Genomic organization of alpha1 and beta1 subunits of the mammalian
            soluble guanylyl cyclase genes
  JOURNAL   Proc Natl Acad Sci U S A 97 (20), 10878-10883 (2000)
   PUBMED   10984516
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121563.3, AK139406.1, BC050945.1 and AC131757.3.
            
            On Jun 17, 2009 this sequence version replaced NM_017469.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF297083.1, AK147128.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                AC121563.3         102182-102219       c
            39-1955             AK139406.1         2-1918
            1956-2235           BC050945.1         1917-2196
            2236-3251           AC131757.3         134994-136009       c
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 35.97 cM"
     gene            1..3251
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /note="guanylate cyclase 1, soluble, beta 1"
                     /db_xref="GeneID:54195"
                     /db_xref="MGI:MGI:1860604"
     exon            1..140
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     CDS             138..2000
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /EC_number="4.6.1.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     soluble guanylate cyclase beta-1 subunit; guanylate
                     cyclase soluble subunit beta-1; NO-sensitive guanylyl
                     cyclase beta 1 subunit; GCS-beta-1; GCS-beta-3; soluble
                     guanylate cyclase small subunit; guanylate cyclase soluble
                     subunit beta-3; beta 1 sGC; guanylate cyclase 1, soluble,
                     beta 3"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit beta-1 isoform
                     1"
                     /protein_id="NP_059497.1"
                     /db_xref="CCDS:CCDS38460.1"
                     /db_xref="GeneID:54195"
                     /db_xref="MGI:MGI:1860604"
                     /translation="MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYD
                     DSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLD
                     ALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGT
                     EIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFP
                     FHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVF
                     VLRSKEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLT
                     RRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDT
                     LLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLL
                     NDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAG
                     QVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSE
                     YTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETNEEDEN"
     exon            141..214
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            215..315
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            316..434
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            435..632
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            633..863
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            864..980
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1114
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1312
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..1550
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            1551..1691
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1846
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            1847..1973
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..3251
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3189..3194
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /note="hexamer: AATAAA"
     polyA_site      3212
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /note="major polyA site"
     regulatory      3231..3236
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /note="hexamer: AATAAA"
     polyA_site      3251
                     /gene="Gucy1b1"
                     /gene_synonym="GC-S-beta-1; GCbeta1; Gucy1b3"
                     /note="major polyA site"
ORIGIN      
        1 agagttggcc gagactgatg ccctagcttc agctctgtgc tggtctggga gcactgtcgc
       61 cgctgtagtc gctgtctggg tcccttccgc ggtactcttg cctggaagct gcttcctagg
      121 ctccgggcgc ggacaccatg tacggtttcg tgaaccatgc cctggagctg ctggtgatcc
      181 gcaattatgg tcccgaggtg tgggaagaca tcaaaaaaga ggcacagctg gatgaagaag
      241 gccagtttct tgtcagaata atatacgatg attccaaaac atatgacctg gtggctgctg
      301 caagcaaagt cctcaacctc aatgctggcg aaatcctgca gatgtttggg aagatgtttt
      361 tcgtcttctg ccaggagtct ggctatgata ccatcttgcg tgtcctggga tctaatgtca
      421 gagagttttt gcagaacctc gatgccctgc atgaccacct cgccaccatt tacccaggga
      481 tgcgcgcgcc ttccttcagg tgcaccgatg cggagaaagg caaagggctc atcctgcact
      541 actactcgga aagagagggg cttcaggaca tcgtgatcgg gattatcaag actgttgctc
      601 aacagataca cggcactgag atagacatga aggtgattca gcaaagaaat gaagaatgtg
      661 atcacaccca gtttttaatt gaagaaaagg aatcaaaaga agaggatttt tatgaagatc
      721 tggataggtt tgaagagaat ggtacccagg aatcacgtat cagcccttac accttctgca
      781 aagcatttcc ttttcacatc atatttgacc ggaacctagt ggtcactcag tgtggcaatg
      841 ccatctacag agtgctcccc cagctccagc ctgggaactg cagccttctg tctgtcttct
      901 ctctggtccg ccctcacatc gacatcagtt tccatgggat tctttcacac atcaatacag
      961 tctttgtact gagaagcaag gaagggttgc tggatgtgga gaaacttgag tgtgaggatg
     1021 aactgactgg agcagagatt agctgcttac gtctcaaagg ccaaatgatc tacttaccag
     1081 aagcagatag catcctcttc ctctgttcac caagtgtgat gaacctggac gacctaacaa
     1141 gaagaggcct gtatctgagt gacatccctc tccacgatgc tacccgagac ctggttcttt
     1201 tgggagaaca gttccgggag gagtacaaac tgacacaaga gctggaaatc ctcaccgaca
     1261 ggctgcagct cacactgaga gccttggagg atgagaagaa aaagacagac acattgctgt
     1321 attctgtcct tcctccatct gttgccaatg agctgagaca caagcgccca gtgcctgcca
     1381 aaagatacga caatgtgacc atcctcttca gcggcattgt gggcttcaat gctttctgta
     1441 gcaagcatgc atctggagaa ggggccatga agattgtcaa tctcctcaac gatctctaca
     1501 cccgatttga cacactgact gattcacgaa aaaacccatt tgtttacaag gtggaaacag
     1561 ttggtgacaa gtatatgaca gtgagtggct tgccagaacc ttgtatccac catgcacggt
     1621 ccatttgcca cctggcttta gacatgatgg aaattgctgg tcaagttcaa gtagatggtg
     1681 aatctgttca gataacaatc gggatccata ccggggaggt ggtgacaggt gtgattggac
     1741 agcggatgcc tcggtattgt ctctttggga ataccgtcaa cctcacaagc aggacagaaa
     1801 ccacaggaga aaagggaaag ataaatgttt ccgaatatac atacaggtgt ctcatgtctc
     1861 cagaaaactc ggatccactg ttccatttgg agcacagagg cccagtgtct atgaagggca
     1921 agaaggaacc aatgcaagtc tggttcctat ccaggaaaaa tacaggcacg gaggaaacaa
     1981 atgaggagga tgaaaactga gtgtcaggtt gtggaacaaa gagaacgtcg gttgggttcg
     2041 ggtgacagtc taatgtgtgt caagcaagga gcacttcttc cctgtggata gcaatttcta
     2101 cttcgtgttt tagtggccca aggctttctc ctagttacac agatctcaca ctatggttta
     2161 tttgatttta gctctgcttt cgattacttt taaggtctca gtatattttc caaagtttgg
     2221 gtttttgatg tggatgactt gagctgtttc ttaaattctg ctacaagcat tgcctagacg
     2281 gacgttctaa aagtgataag cacccactgt gttaagtttg ttaaatctga tagaacgaga
     2341 cttaatagta tctggccatg cgtgtatata tcatggctca gtagatttgt tttatgctcc
     2401 atgtatatgt gtgtgtatat gtatatttta atgactatac cataaaacaa agtttatatc
     2461 atgttggtgc atggcattct agaaaccatt ttgtacacga gtgaatctaa gttttaggga
     2521 aaaaaggcaa tttatttgta gacttctgaa gtaagaatta gtatgctata ttaggaaaag
     2581 gagtgactat tttgaagtat gtcaattccc ttctgggact ctattattgc aaaaattggt
     2641 tgctcattca aattttatgc caattacatt ttatctaaca tctacattgc cctaatttgt
     2701 actgaagtcc ttgtatattg tgtttggttg actactggta gctgtgatgg gggctgcatt
     2761 gtttcacact gaagggttac atttgcttta gcaagtgttt ggggtcaaac tatgtccagg
     2821 aacaaggctg ggaatacata gctaggacac tgctgttgga ggccccaccc cagcccccga
     2881 acctgcccct ggcctcgctc caggcttgag cttctttatt agcttagaag gatgtcaact
     2941 tatccaggat atcatattca gaatattcat caaaatcatt ttaattctag catagaatgg
     3001 actgaattcc tgttggtata tttggcctat catcctttaa atgtctctga taatttattg
     3061 atatctatct ttataaaata gaaaaaaagt acttttgtgt aaagatattt gtctttaaat
     3121 ttagtatttc atatcagcac atcaatgtat gtataaatgt tacatgttaa ttgtgtaaaa
     3181 gattctacaa taaattattt ttaccacttg tctacagtgc gcattaattt aataaaatgg
     3241 tattgacact a
//
