LOCUS       NM_001033322           13727 bp    mRNA    linear   ROD 06-AUG-2023
DEFINITION  Mus musculus guanylate cyclase 1, soluble, alpha 2 (Gucy1a2), mRNA.
ACCESSION   NM_001033322 XM_001002873 XM_001002880 XM_915020 XM_923534
            XM_972907
VERSION     NM_001033322.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 13727)
  AUTHORS   Wang F, Ma Q, Dong X, Wang T and Ma C.
  TITLE     circ-Gucy1a2 Protects Mice from Cerebral Ischemia-Reperfusion
            Injury by Attenuating Neuronal Apoptosis and Mitochondrial Membrane
            Potential Loss
  JOURNAL   J Invest Surg 36 (1), 2152509 (2023)
   PUBMED   37277119
  REMARK    GeneRIF: circ-Gucy1a2 Protects Mice from Cerebral
            Ischemia-Reperfusion Injury by Attenuating Neuronal Apoptosis and
            Mitochondrial Membrane Potential Loss.
REFERENCE   2  (bases 1 to 13727)
  AUTHORS   Neitz A, Mergia E, Imbrosci B, Petrasch-Parwez E, Eysel UT,
            Koesling D and Mittmann T.
  TITLE     Postsynaptic NO/cGMP increases NMDA receptor currents via
            hyperpolarization-activated cyclic nucleotide-gated channels in the
            hippocampus
  JOURNAL   Cereb Cortex 24 (7), 1923-1936 (2014)
   PUBMED   23448871
REFERENCE   3  (bases 1 to 13727)
  AUTHORS   Neitz A, Mergia E, Eysel UT, Koesling D and Mittmann T.
  TITLE     Presynaptic nitric oxide/cGMP facilitates glutamate release via
            hyperpolarization-activated cyclic nucleotide-gated channels in the
            hippocampus
  JOURNAL   Eur J Neurosci 33 (9), 1611-1621 (2011)
   PUBMED   21410795
REFERENCE   4  (bases 1 to 13727)
  AUTHORS   Wang H, Kohr MJ, Traynham CJ and Ziolo MT.
  TITLE     Phosphodiesterase 5 restricts NOS3/Soluble guanylate cyclase
            signaling to L-type Ca2+ current in cardiac myocytes
  JOURNAL   J Mol Cell Cardiol 47 (2), 304-314 (2009)
   PUBMED   19345227
  REMARK    GeneRIF: These data suggests a novel physiological role for PDE5 in
            restricting the effects of NOS3/sGC/PKG signaling pathway to
            modulating beta-AR stimulated I(Ca), while limiting effects on
            cardiac contraction.[sGC]
REFERENCE   5  (bases 1 to 13727)
  AUTHORS   Taqatqeh F, Mergia E, Neitz A, Eysel UT, Koesling D and Mittmann T.
  TITLE     More than a retrograde messenger: nitric oxide needs two cGMP
            pathways to induce hippocampal long-term potentiation
  JOURNAL   J Neurosci 29 (29), 9344-9350 (2009)
   PUBMED   19625524
REFERENCE   6  (bases 1 to 13727)
  AUTHORS   Vanneste G, Dhaese I, Sips P, Buys E, Brouckaert P and Lefebvre RA.
  TITLE     Gastric motility in soluble guanylate cyclase alpha 1 knock-out
            mice
  JOURNAL   J Physiol 584 (Pt 3), 907-920 (2007)
   PUBMED   17717014
  REMARK    GeneRIF: The aim of this study was to investigate the role of
            Gucy1a2 in nitrergic regulation of gastric motility in male and
            female Gucy1a2 knock-out mice.
REFERENCE   7  (bases 1 to 13727)
  AUTHORS   Schmidt H, Stonkute A, Juttner R, Schaffer S, Buttgereit J, Feil R,
            Hofmann F and Rathjen FG.
  TITLE     The receptor guanylyl cyclase Npr2 is essential for sensory axon
            bifurcation within the spinal cord
  JOURNAL   J Cell Biol 179 (2), 331-340 (2007)
   PUBMED   17954614
REFERENCE   8  (bases 1 to 13727)
  AUTHORS   Nimmegeers S, Sips P, Buys E, Brouckaert P and Van de Voorde J.
  TITLE     Functional role of the soluble guanylyl cyclase alpha(1) subunit in
            vascular smooth muscle relaxation
  JOURNAL   Cardiovasc Res 76 (1), 149-159 (2007)
   PUBMED   17610859
REFERENCE   9  (bases 1 to 13727)
  AUTHORS   Haghikia A, Mergia E, Friebe A, Eysel UT, Koesling D and Mittmann
            T.
  TITLE     Long-term potentiation in the visual cortex requires both nitric
            oxide receptor guanylyl cyclases
  JOURNAL   J Neurosci 27 (4), 818-823 (2007)
   PUBMED   17251421
  REMARK    GeneRIF: Requirement of both alpha-2 and alpha-1 guanylate cyclases
            for signaling in long-term potentiation (LTP) indicates the
            existence of two distinct nitric oxide/cGMP-mediated pathways,
            which have to work in concert for expression of LTP.
REFERENCE   10 (bases 1 to 13727)
  AUTHORS   Mergia E, Friebe A, Dangel O, Russwurm M and Koesling D.
  TITLE     Spare guanylyl cyclase NO receptors ensure high NO sensitivity in
            the vascular system
  JOURNAL   J Clin Invest 116 (6), 1731-1737 (2006)
   PUBMED   16614755
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122444.4, AK147527.1, AK165150.1 and AC156642.2.
            
            [WARNING] On Apr 12, 2024 this sequence was replaced by
            NM_001033322.3.
            
            On Jan 27, 2012 this sequence version replaced NM_001033322.1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219380.73272.1,
                                           SRR11927932.2518309.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-429               AC122444.4         153647-154075
            430-1674            AK147527.1         2-1246
            1675-4603           AK165150.1         36-2964
            4604-13727          AC156642.2         178913-188036
FEATURES             Location/Qualifiers
     source          1..13727
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 2.14 cM"
     gene            1..13727
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /note="guanylate cyclase 1, soluble, alpha 2"
                     /db_xref="GeneID:234889"
                     /db_xref="MGI:MGI:2660877"
     exon            1..751
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             455..2647
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /EC_number="4.6.1.2"
                     /codon_start=1
                     /product="guanylate cyclase soluble subunit alpha-2"
                     /protein_id="NP_001028494.1"
                     /db_xref="CCDS:CCDS40526.1"
                     /db_xref="GeneID:234889"
                     /db_xref="MGI:MGI:2660877"
                     /translation="MSRRKISSESFSSLSSDYLETSPEEEGECPLSRLCWNGSRSPPG
                     PPGSREAAKAATSAPAASAAAASAAVAAEYKRAQRRGRVNLDSLGESISLLTAPLPQT
                     IHMTLKRTLQYYEHQVIGYRDAEKNFHNISKRCSYADHSNKEEIEDVSGILQCTANVL
                     GLQFQEIQERFGEEFFKICFDENERVLRAVGSTLQDFFNGFDALLEHIRTSFGKQATL
                     ESSSFLCKELPEGTLMLHYFHPHHTVGFAMLGMIKAAGKRIYHLNVEVEQIENEKLCF
                     DGSNPSNCSCLTFLIKECETTQITKDNPQGTSQVPADLRISINTFCRAFPFHLMFDSN
                     MVVLQLGEGLRKQLRYDTHRALKFEDCFEIVSPMINATFDRVLLRLSTPFVIRTKPEA
                     SGTENEDKVMEIKGQMIHVPESSAILFLGSPCVDKLDELMGRGLHLSDIPIHDATRDV
                     ILVGEQAKAQDGLKKRMDKLKATLERTHQALEEEKKKTVDLLYSIFPGDVAQQLWQGQ
                     QVQARKFDDVTMLFSDIVGFTAICAQCTPMQVISMLNELYTRFDHQCGLLDIYKVETI
                     GDAYCVASGLHRKSLCHAKPIALMALKMMELSEEVLTPDGKAIQMRIGIHSGSVLAGV
                     VGVRMPRYCLFGNNVTLASKFESGSHPRRINVSPTTYQLLKREDSFTFIPRSREELPD
                     NFPKEIPGVCYFLELRTDPKPPKPSLSSSRIKKVSYNIGTMFLRETSL"
     exon            752..813
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            814..935
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1654
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..2140
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2284
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2439
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2440..13727
                     /gene="Gucy1a2"
                     /gene_synonym="6330407I18Rik; A230060L24Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaggcggagg aaccttcctg caccccaatc cctgattccc tcagctccgc agcgagagcg
       61 gtgcgcacag gcgggccctg aggctgcgcc tgtagagcag cagctgcaga ggcgacagtg
      121 gcgggtcccc agctcatctc cagccccact gctgagcctg ggccccaagg agccgctgca
      181 atccaagttc ccgccatgag cagctgtctc gaggggctca gcggccccag ggactcgcgt
      241 ctgggcgagc ccgaacgcaa cgccaagcgg ccccgacgct cctgaagctg cctgccacag
      301 caggtcccac caaggcccca gacaccactg cgtccccgcc tccaccacca ccaccaccac
      361 caccaccacc accaccacca ccaccaccac caccaccacc acccaccacc acctccgctt
      421 ccgctgtctc agatgcaata gtgtctccag cagcatgtct cgaagaaaga tttcatccga
      481 gtctttcagc tctttgagct ctgactacct ggagaccagc ccggaggagg aaggggagtg
      541 cccattgtcc aggctctgct ggaatggcag ccggagcccg ccagggccac ctggctctag
      601 agaggccgct aaggctgcca cttcggcccc cgctgcctct gccgccgcgg cctctgctgc
      661 agtcgccgcg gagtacaaga gagcccaacg ccgcggaagg gtcaacctgg actcactagg
      721 cgaaagcatc agcctcctga cggcgccctt gcctcagacg atacacatga ctctcaagag
      781 gacattgcag tattatgaac accaagttat tggttatagg gatgcagaaa agaatttcca
      841 caatatctct aaaagatgct cctatgcaga ccattccaac aaagaggaaa ttgaagatgt
      901 ctcaggaatt cttcagtgca ctgctaatgt actcggactg cagttccaag aaattcaaga
      961 aagatttggt gaggaattct ttaagatatg ctttgatgag aatgaaagag tccttcgagc
     1021 tgtgggcagc acattgcagg acttcttcaa tggctttgat gcattgttag aacacattag
     1081 gacttctttt gggaaacagg ccactctaga gtcatcatct ttcctatgca aagagcttcc
     1141 tgaaggtact ctcatgctcc attacttcca ccctcaccat acggtgggct ttgcaatgct
     1201 gggaatgatt aaggctgcag gaaagagaat ctatcactta aatgtggaag ttgaacaaat
     1261 tgaaaatgag aaactctgct ttgatggttc aaacccaagc aattgcagct gtcttacttt
     1321 ccttatcaaa gaatgtgaaa ctactcagat cacaaaggac aatccacaag gtacttccca
     1381 agttcctgca gacctcagaa tcagcatcaa caccttctgc agagccttcc ccttccattt
     1441 gatgtttgac tctaacatgg tggtccttca gcttggggag ggcctacgga aacaacttcg
     1501 atatgacact cacagagcac tgaagtttga ggactgtttt gagatcgtct ctccaatgat
     1561 taatgccacc tttgatagag tcttgctacg attgtccact ccatttgtta taaggaccaa
     1621 gcccgaagct tctggtactg agaatgaaga taaggtgatg gaaatcaaag gacaaatgat
     1681 ccatgtccca gaatcaagtg ccattttgtt tttgggatct ccatgtgtgg acaagttgga
     1741 tgaactgatg ggccgaggac tgcatctctc agacatccct attcatgatg ccacccgaga
     1801 tgtaatttta gttggagagc aagccaaggc ccaggatggc ttgaagaaga ggatggataa
     1861 attaaaggcc acattagaaa gaactcacca ggccctggaa gaagaaaaaa agaagacagt
     1921 ggatctgctg tattccattt tccctggtga tgtagcccag cagttgtggc aagggcagca
     1981 agtacaggcc agaaagtttg atgatgtcac catgctcttc tctgacattg tgggctttac
     2041 agccatatgt gctcagtgta ctcccatgca ggtgatcagc atgctcaatg aactctacac
     2101 aagatttgat caccagtgtg gccttttgga tatttacaag gtggaaacaa taggggacgc
     2161 atactgtgtg gcatcaggac tgcacaggaa aagcctatgc catgcaaagc ccattgctct
     2221 gatggcctta aagatgatgg agctttcaga agaagttctg actcccgatg gaaaagcaat
     2281 tcagatgcgg ataggcattc actcaggctc tgtgctggct ggtgtggtcg gagtacgaat
     2341 gccacgatat tgtctttttg gaaacaatgt cactctggca agcaaatttg aatctggaag
     2401 tcatcctcgg cgtatcaatg tcagccctac tacttaccaa ttactaaaac gagaagacag
     2461 tttcacattt attcctcgtt ctcgagaaga acttccagac aactttccga aggaaattcc
     2521 tggggtctgc tatttcctgg agttaaggac tgacccaaag ccaccaaagc catccctatc
     2581 ttcatcgaga ataaaaaagg tttcctacaa tattggcaca atgttcctcc gggaaacaag
     2641 cctctgagac ttgctgcaga tcaaagactc ctccaaaaag cacaagccca gacatgggtc
     2701 ctcatgagga gaggaagaaa ctatttctct tccaatgctc tgtaagaaca tatagtaggt
     2761 tttgtgttaa cgtcaggcaa taatccttga aatggtgaat aactgatgtc aactaaaaac
     2821 aaaaagctgg gtggtgtggg ggaaaagtat aatgtttcca tccatatgag tatttctggt
     2881 aatggatatt ttatttacaa gtacaggcaa tcaaaagtaa ctaatagagc ttatggtttt
     2941 ttgtttgtca gccatgcagg tatctctccc tatacattta taccactttt gaaagccagt
     3001 tttaaatata tattcctaca tttagtgaac tgataggtaa ggaataatct ctaattttct
     3061 ttttctgaat aggcatctca tacatatatt atggcagtgt gattaaactt ccaagcagaa
     3121 gaactataaa ttagctaaat attttaggga tgttacctat ttttatatat ctataactat
     3181 atcttctcct agataattgc atttcccaca catgattaaa tattcaaaat gtgctggggc
     3241 tttgtgggta gatttgatga tgctatatac aagtttcact tacttagaga gattagtgct
     3301 tgaactggga atatgtataa ctaaggatat cagctgttga ggaaacatgt gtttgcttag
     3361 taaacatacc ttttagattt atttcatgta gcttggatcc agaggaaatg gaattttcta
     3421 gtagaaaata aacctgtaac taagcttcag gaaagatttt cttttaaatc taaacacttg
     3481 taagttaaaa caaaacactt tgcaaaacta gatccttttt acattaaaac ttaccaatgt
     3541 aatggtgtat gcaatcttaa gtggctccat aatcaacatt atctctcaaa atgatgtatt
     3601 agtagctgcc cttcctgtct ctgacctatg ccttcaaaga actgactgtc acactccaaa
     3661 gcaaattctg atatcagagt tactgacgaa tatctgtcag gcaattttgc caagaatgtt
     3721 gtttccaatt gcctaacaac aaagcaaaga aaagctcgat ttcatgcctt ttggattcta
     3781 aaatgtatat tctttggagg ttttgttgat acaggctcta aagtatcaaa ctgcctcatg
     3841 aatactggtg taggctgaac ctccagattc cacaaacacc atgtatacat agatagcatt
     3901 ccattgcatg ctctactaaa ttaaggactg ctatcaatag ccaacactgc atgaaaatac
     3961 tctttacagg ctcacactga aattaggagt gcacagtgtg caagaatttt catatttgga
     4021 gccacatata aaacctacat atctaaggag tttagtgatc tggaagcatt tgctgtattg
     4081 aaataatagg atggaccttt tcttcttact caagtatcca gtatgtattc tcactggtag
     4141 gtatgtttac tggacacaat atatactttt tttcaggaat ttgggcctgc attaaacatt
     4201 ctaaggtatt gctatatgtt aattcccaaa gtcgtctgtg attgttaatc actgttctct
     4261 atgtgacttg gcaacattta aattaggcaa agcacttgtt ctattcatga tctttttcta
     4321 actttagctg tattctcacc ctggtataat tgagatacta atttaacata ctttacatgg
     4381 agaaagcaag atatatattt tccaggttct atgctatcac ctgtctcttt tttgccaagc
     4441 atagttctgt ttttacacca tacagataaa gggccagagt atgcctgtga tgctttgtag
     4501 cctgagggca atgtatcctc aatagaataa taacaaatac atttcaagaa acttgaatat
     4561 tagcttacct ccttcatagt agagaaggat gaggagtgta gggagagtag atagagtaag
     4621 ctgaaaacac aaagtagtct tgttctacta ctgatgaaaa tgcctgatga aagctgacag
     4681 tctcttacta ttgaagaaca ggggaaacag ccatgctcac catagtcaat tatatctatt
     4741 atgtgcagta actatgttta ttatgatata ttcaattatt ttacactcaa taaacatgtt
     4801 ctataaccat aaggttgaaa gaatatttcc caggaatcat gcaatatatt ctctgtattt
     4861 aagaaaattt atgaaaaata tacacacacc cccacactca tatcttttaa aacctaatat
     4921 ttgaaatcca ctctattatt cagtgattaa actattacta aataaacaat taaataaagc
     4981 tcaagaggag aatttactca tttattctca gttttaaaag catcacaaat tctgtatttg
     5041 gttacattat tttacatagt taggaaagag ccgttacact gtcagaaatg gcattttaaa
     5101 gaacattgca taaatttatt atatatccct aacaggaaat tatattcttc aatcactata
     5161 atctatattt ctgaagacag tctgaggaag taatataatc tcttgtaagt tttctagact
     5221 gtttcaaatg accatcatag ttacattctt ttttgcttta tgtgcttgta aggcactcaa
     5281 aaaaaaaaag ttccattgtc tgcatttgta taagggagaa gctatgaact ttatatttcc
     5341 atcttttatt ttctacaaag cagagttcat atgttctatc ttagaaaact tggctgcaaa
     5401 tatgtgacag tattttgatt ctattgatta atagctctct attactttca tatatttaac
     5461 aacatgtagg aatggtatac cttttagtgt gtcaggttga aaggaatcta ggaaggtatc
     5521 catagaaacc aagtgattgc aagttggtgt aatatgttta gctcactgta aaatcaactt
     5581 caaatctaac atggcatgaa taacttccct ttctgaatat tggatcaggt agttaattta
     5641 ggaaaggaac tttgcaacta gacattatta ctaacattta ccactgacag aaatatcatt
     5701 tgatgaggag aacattgtca tcttcaaata agtcaataat ctagcatagg tataatcttc
     5761 atatacttgt atagaagcga tcttatggac tttagtaaag aggcaatggt gttcacaaag
     5821 aaaaattacc atgtctgaga aacattgttg ggactttcag atttgtcatc ttgtgaaggt
     5881 gatctatgac ccaactttct aaaatctacc tctgtatgta tatctcctgt tgtattttag
     5941 actaattggg gtatctcttc caattccata ttttatgtgg agaccagtaa gctgtgaccc
     6001 atgtttctaa caccatctct gatatataaa attggagagt tagtcagtgg atccagcaca
     6061 cattagttct tacagtctca aacttttctc tcttgtttaa tgaccatgac attaggatag
     6121 agatatatgt tcagaagtgt gaaagtttgt gtctctttaa ggaaagaaaa aagatattaa
     6181 tgagttaaac aaaatctaat tctcaattgt agcaatgctt taaaacatgt aaatttttcc
     6241 agagagatct accaataaag caaaagtaaa cagaaacaaa aggcaaagcc atttacaaaa
     6301 ccatccaact ctattcagga cagaacagtg tttctgctga ctgaaaggtg ggtctacagg
     6361 tgtgcagtgt taatatggac ttggagatta tggccatcaa cactggcagt ttcttcagtt
     6421 tgccactggc tgaaatgggt gttgtacata tataacatag aacttaaata gtgacaaagg
     6481 tttctgtact cttttttttt tttagcaaga cattgattat cccttcctca ccctctgtgt
     6541 gctcaacaga tattatctca acctctcaag cccagccttc aaaaacatca tttcttcttt
     6601 acttctgaac cagatttaat acacatataa gttatataaa catgctgaaa caaaattcat
     6661 gttaagaaaa taaaatgtcc ttatatgttg cctcagatca gaatcctcac agtggtgaat
     6721 gaaaaacaac ttctgatact ctatatacat taacataatc agcagagact gttgagatag
     6781 tatgcaatag tccttcatca cacttgattc aaaaaaacct aagtactagt gttctaccat
     6841 ctttgttctc cctttctttt cattgaaaaa aaaatgacca catcttgtct tataaagtaa
     6901 tgaatctcgt gtgtgtgcac atgtgcaccc ttgcatgtgg ttattggttg taaaaggtca
     6961 tttgagaagg acttgagaat gtgtgtaagt agtttgtctt tgttctgata tgttgtatag
     7021 ccctaaaata ctttcagtca agtacttcat tgctctccca cactgtccta gtttaatatt
     7081 tacttttctg cttaaaagta tttcactggc tgcataataa taatctagga agttttagga
     7141 attggaatta ctgggaatat cctacggcac atggcagagg taggacccaa gaatgagttt
     7201 aggaaaccac cctcattaat actgcataca gccagatgtg gagattatgg aagaacatgc
     7261 ttacaatctc agtgactcaa aggtaataca agttgactaa gatagcaata tactctttta
     7321 tttaattttg aatatttaag cactctgggt ttcttatcat aaattttaat tttagctttg
     7381 gtttcaaatg cttagtatgc tttcagaaca tgaatcttca aacttattag tgacagaagt
     7441 caacactgat tagcatgagg gatgaatatt gctcagtatt attaggcata gtagctatat
     7501 tcttggttac tggtaaagct gtgcagtaca cttgctggcc ttcaccactg aagtaacaga
     7561 aaataagttt aggacataag taagtattga aatgacagcc taccttcaga atgctgttat
     7621 ctatattata aaaagatgca aagttaatac ttaatgcctc gtttcattat gtatatcaca
     7681 tatttctctc aaagcaattt cttagcaata cttataacta tcttttaagc aatattttaa
     7741 gccatggtag tgatgccata tataatgcac acgtgtgcat ttcaccaagc ctttggagaa
     7801 atggagacat ggtttccact agcaataata caggtggttt ggaattttta aagttatttg
     7861 tcgtttcaca acataatgaa aagcaaagga tgaatcatat ttaaaaactg atcaaatagt
     7921 tcacaaatca atctcaaaac tcactaattt gtggacatgt agctaatgag tttacatcag
     7981 agtatgtctg gtgtaattta aaactatcta tattactata atttctattg ctctcaaaga
     8041 catttacgta ctatttgtaa agttgctcaa atttttggat ctctgtagtt tattacagca
     8101 agcaacaaag atggaagtag agtgtaagtt caaagagctg attgagaaag tgaactttga
     8161 taaaatatac gtgagtcttg gctttataag ctcctgaaag atttaagata aaagagcaaa
     8221 gagatttcta aagtaataga gactgttaga agagagagga tcttaaggaa tatggagttt
     8281 ctatagtata cacagttgat ttaatgaatg ctagataacc cttttgctat gtgttacttt
     8341 tgtgtttttt tgtttgcttt cttgttttgg tcattttgtt ttgtttggtt tgagagaaaa
     8401 aattccaaac taaccattcc cagtcctctg atgaggtagt gtcagggtag cagtagctgt
     8461 aaggctcagt aaaacttcaa ttagaagctc acagagccat aaatagcaag tctctgaagg
     8521 tggtagataa aatgattctg ggttatagtg acaaaggtaa gtagaagtgc cagaacatcc
     8581 ggatctacaa aacagagggt caaacattgg ctctgcctta ggttacagag aagcccactg
     8641 aaattagcac aggacagata gtcagtgtgc agaggacatt aaacacagca tcttcacaca
     8701 cgtttggtcg acatgacatt cactagcaaa atgattttgt actttacgct cctccaactg
     8761 ctgaaatagg aatgatatgt ttttattatt gtgaacttga atcacaaagt ctggaaaaca
     8821 ttttacgtcc taaaagcttg tgccatcatt tttgaggtta ttccatagtc aatacttcat
     8881 attttctcaa tttgcactct actaacaaag caagtactca gcttctgaga gaaaaaaaaa
     8941 tggcctaggt ttactcaaga tggaagcccc agagagctag agaacccaag accattagat
     9001 agtgcttagg ggaaaaatga gggtgttctc tctgccttct aacattgaaa gcaaatggat
     9061 actctgtcat tatcactcag agatttctca cttacttgta actgactatc tcatgcagaa
     9121 tgcatttagg agaagtaagt tccctcctat ttttaactag aattactagt cttaaaaaat
     9181 caattattat attgtgtcag ttcacttgta tataaacttc aaagttgata ggttttgaaa
     9241 tcgtatttca taaaatacat caaaagcttt agcaatgaga acctaattct gtggcttagt
     9301 ccttcaggtt ttaacctctg gtgatatcac ttaatcgtaa attaattata gttcagtgct
     9361 tgttattttt ccaagttttc tatgttacta aacaaggttg ttataaataa gaattttaaa
     9421 tggtggcatt gaaccttatt tctcttgttg aacaatattc tcatgcttaa aacattaaac
     9481 tattacagag ctcgatatta aaagccaaag gtagtgtgtt cagactcctt catgttaagg
     9541 catctaacaa agcaccccag tgatctagat aatgcacagg aattgagaat tatctaaagc
     9601 aattatgtgg gagctttaga aacagtcttg aacatttgtg taaggataat gatgagtaga
     9661 tagcagccat gattatcagg cctattattt ttattaattt cagatgtgtc agcatcactt
     9721 gcaaaattca tttcttgtgt aacttcctga tctgaaataa tggttacctc ttgcttattc
     9781 ttttgatcct taaaaaattt ttactgtcat gaaatacaaa ttattatctg accataacag
     9841 tttttctaat ttatgagcat ataagatata tttaggtgga gtcagctgga gggaaagaaa
     9901 gagaaagaaa aagcccccct atagggaagc ttttacttac aatgggaaga tgaaaattat
     9961 gtctgtagtc catctttgtt aacctaagag aaagctaaag atattgatat ggatatatca
    10021 ttaaagattt tttttaaaaa ataagaaata atactgaatg ataggtacac caaataaaat
    10081 tcagcatgtc aaagctttag aaaacaaata agaaacttaa taaaactgtt aattagaatg
    10141 actatttaaa ggaacaatga caaaaataaa tcatcatata tatctaccaa agcagaattt
    10201 gcttctgttt ggaaaattta gtaccatgtc ttcaatcagt attttctgtg acaagctaag
    10261 ctctagtata acctttatgc taatgagaaa ccaggtagga attcaaatat catctgtatg
    10321 agctgcagtt gcatcttaaa tataataggt ctggtatcat ttctaaataa ctctcaataa
    10381 aagtctaatg aatacattgt gtacaaagag actacgtagg gcaaactaat caggtcttta
    10441 aatacattgg cactttaaac tcctgtatgc ccctctctcc tttctaactc cagtccttct
    10501 attctttcct atttcacttt attctgtctt ttctgtctct atttcttaac agctcttata
    10561 aagtgcatgg caatattgtt ttactctttg agctgaggct tttccttttc acatttacat
    10621 gactgaatat tatagacctg tgaggtaagg aggtaaaagt tagaacatag tacagatcat
    10681 ttgaaaagag aagtgactgt gaatttggat cttgactgct gagggctttg tcatacaaga
    10741 gaaagtggat ctatgcaaac ttctttgctc tctcctggga agtatcagaa tcaacatttt
    10801 aaagctcctg cccattgccc ctgcaagtct tcattcagaa aatcctcttc tagcttaatc
    10861 tctaattttt cattagattt gagaatcaca ctgctaaaga ttgagataga aaagcctttt
    10921 ctcaaatctg cttttagaat cataggtaca tgaaatgcaa gttgatttta ctggaccttt
    10981 agatgtaagg gatacactac caaacttctt gggtactgtt acatgagtca gatagttctg
    11041 ttatagtgtt tctagatctt gaagtgtctg acaaaattca acatagaatt tatatctacc
    11101 ctgctgagaa agcaataagt agcattctcc atgtttctta gctctaccct tttaaaattt
    11161 taacacattt acatgtaact ttttaagcac agctttgact tattttctag aattaatgaa
    11221 tttccatggt ttgaaataaa aagacctaat tttgatattt catattgtta tagcaaagga
    11281 tttatgtatc tcaatccaag ccatttattt ttcagggaaa attagataat gcctagcaaa
    11341 gtatttgatt tataacagaa agtaattcaa atatcttaag tgtactaaaa aagaaggcaa
    11401 aaatggaatt ctaggaaata catctaatca ttttaatttt cttcaattga aatgtcttat
    11461 atgttatgtc actgtataaa tattatattc aaatatccat agaatacctt tgtttgtatt
    11521 ctctagtggt atatgagtgt tattattttt tttctattta gaaagatata ttttcaaaat
    11581 caacattgta tacctggtat ttctgacctg gggatgtaat aagtgcccca tctgggaact
    11641 accctcctca atgcaacctt ttacttttca gttctgattt ttaattatat cctttatcag
    11701 aagtcttaaa ttcatatcaa agaaaagaag aagtatataa gttccaaaca aataaacacc
    11761 ccccaaacag caacacattt ctttgtcatc ttctatatat cccattcttt acacttaatt
    11821 tctgagatag gtgcatattt accatctaaa ttgaaactta agtgaacact ttttgtgttt
    11881 tcttataatt ttcctaatta agtatagcca atcttacaac ttaatcaggt aaataaatga
    11941 ataactttaa ttaaaataaa tattaaaata aactttaaat atccttatca attttattta
    12001 cttacagaaa taattttaaa gtgattttta aatgggaaaa atatagataa tgcaatggag
    12061 aaattaagct gtgagccaaa gcaccttttt tgtctctatg tggtttttac ttcctactga
    12121 agatcaattt ctaagattcc attgtccaaa atgtacatgc tactggccac agactttaga
    12181 gcacccacta cttattagga aatctagtcc tgctgttaca gctggtcagg atgtgagaaa
    12241 attaagaaga attggtgaga aaatagtcca tgaagagaaa tgttccttcc ttccttcctt
    12301 ccttccttcc ttccttcctt ccttccttcc ttccttcctt tcttccttcc ttcttttccg
    12361 tgctttctct cttgccttct ttatttcctt ctttccttct ctctctctct ctctctctct
    12421 ctctctctct ctctttcttt ctttctttct ttccctcttt ccctctttct ttctttcttt
    12481 cttttaattt aaattgaatg aacaatttgt ttcccttaga aaagaaaaag aaaatattct
    12541 ttaaagtttg tctttgaaaa tgtacaatta ttgtaatagc ttctgacacc tacactatac
    12601 cttcagtaat caattgacta acctttctaa ttatgctttg aaagcaagcc aattaagtgc
    12661 attcagaaat acatatcatt cctccttcca aaatgaacct acctgaattc agactgttta
    12721 actgtgagtt aactgtgaca ttgtaccaaa gggtcgcaat tcccaagcac tactttaaat
    12781 ctcaaactta ccctgctctt tcaagtgcat aagatcaaca cagcaaacat ttagcatggt
    12841 ttaggaatga ctaggttcct catgctaata taaagacata attatgatgt gctaggcttt
    12901 caatagtgtc ctctgcatga ggtagttttt acttaagtag ataactgagt tattttaaca
    12961 gtttgttcta tttcttgcaa tctgatgagt ctacattttc gcttgacttg ttgtgaataa
    13021 tattatatat atgctatttt actaacagat tatactttgt gatcctttct tttaaaaata
    13081 aatgtagtta tcccaaacat agactataat atcatcaaat aataccaagt aaatatattt
    13141 aaggcatggt taataacata tattttgaaa ggccaagcta tttttttcat atttttattt
    13201 ttaattaatg catctgttaa tcctgtgcca tttttcaaaa gtttcatttt gtggttcaaa
    13261 ttatttctaa aaatattttc ttttacctag tagtacaggt tttgagaaaa atatgattaa
    13321 aatgatcaaa tatgcctcat ttcactagaa ttttaagaat aatacctgta aatgggtgga
    13381 aatattttta aaaataaaac accatatttc tttgtttttt atgtagcatg actttttacc
    13441 tatttgctta ttttacttgt aattatccta agttatgcac aatgtatata ttctctttca
    13501 tgaagcagta gtcattgtta cgtgcagacc acagaatgag gtgagtttgt acatggaaaa
    13561 atgatttatg ggcacctggg attcttctaa aacaaagtgg aaataaaagt tgcctgttat
    13621 atttacatga ctcattacag agctgcataa atcatgtatt atttacctta aaatttcaaa
    13681 atgttacata ttaagtattt aaatcaataa attatacgaa caaaaaa
//
