LOCUS       NM_008191                602 bp    mRNA    linear   ROD 31-JUL-2024
DEFINITION  Mus musculus guanylate cyclase activator 2b (Guca2b), mRNA.
ACCESSION   NM_008191
VERSION     NM_008191.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Ratko,M., Habek,N., Dobrivojevic Radmilovic,M., Skokic,S.,
            Justic,H., Baric,A. and Dugandzic,A.
  TITLE     Role of uroguanylin's signalling pathway in the development of
            ischaemic stroke
  JOURNAL   Eur J Neurosci 56 (1), 3720-3737 (2022)
   PUBMED   35445449
REFERENCE   2  (bases 1 to 602)
  AUTHORS   Pattison,A.M., Barton,J.R., Entezari,A.A., Zalewski,A.,
            Rappaport,J.A., Snook,A.E. and Waldman,S.A.
  TITLE     Silencing the intestinal GUCY2C tumor suppressor axis requires APC
            loss of heterozygosity
  JOURNAL   Cancer Biol Ther 21 (9), 799-805 (2020)
   PUBMED   32594830
REFERENCE   3  (bases 1 to 602)
  AUTHORS   Fernandez-Cachon,M.L., Pedersen,S.L., Rigbolt,K.T., Zhang,C.,
            Fabricius,K., Hansen,H.H., Elster,L., Fink,L.N., Schafer,M.,
            Rhee,N.A., Langholz,E., Wandall,E., Friis,S.U., Vilmann,P.,
            Kristiansen,V.B., Schmidt,C., Schreiter,K., Breitschopf,K.,
            Hubschle,T., Jorsal,T., Vilsboll,T., Schmidt,T., Theis,S.,
            Knop,F.K., Larsen,P.J. and Jelsing,J.
  TITLE     Guanylin and uroguanylin mRNA expression is increased following
            Roux-en-Y gastric bypass, but guanylins do not play a significant
            role in body weight regulation and glycemic control
  JOURNAL   Peptides 101, 32-43 (2018)
   PUBMED   29289697
  REMARK    GeneRIF: Guanylin and uroguanylin, as well as their prohormones, do
            not seem to play a significant role in body weight regulation and
            glycemic control, suggesting that guanylin-family peptides do not
            show promise as targets for the treatment of obesity or diabetes.
REFERENCE   4  (bases 1 to 602)
  AUTHORS   Ikpa,P.T., Sleddens,H.F., Steinbrecher,K.A., Peppelenbosch,M.P., de
            Jonge,H.R., Smits,R. and Bijvelds,M.J.
  TITLE     Guanylin and uroguanylin are produced by mouse intestinal
            epithelial cells of columnar and secretory lineage
  JOURNAL   Histochem Cell Biol 146 (4), 445-455 (2016)
   PUBMED   27246004
  REMARK    GeneRIF: guanylin and uroguanylin are produced by mouse intestinal
            epithelial cells of columnar and secretory lineage
REFERENCE   5  (bases 1 to 602)
  AUTHORS   Folgueira,C., Sanchez-Rebordelo,E., Barja-Fernandez,S., Leis,R.,
            Tovar,S., Casanueva,F.F., Dieguez,C., Nogueiras,R. and Seoane,L.M.
  TITLE     Uroguanylin levels in intestine and plasma are regulated by
            nutritional status in a leptin-dependent manner
  JOURNAL   Eur J Nutr 55 (2), 529-536 (2016)
   PUBMED   25743370
  REMARK    GeneRIF: In obese mice UGN expression was decreased, whereas leptin
            treatment up-regulated UGN levels in duodenum in these genetically
            modified mice compared to WT.
REFERENCE   6  (bases 1 to 602)
  AUTHORS   Rahbi,H., Narayan,H., Jones,D.J. and Ng,L.L.
  TITLE     The uroguanylin system and human disease
  JOURNAL   Clin Sci (Lond) 123 (12), 659-668 (2012)
   PUBMED   22877138
  REMARK    Review article
REFERENCE   7  (bases 1 to 602)
  AUTHORS   Yuge,S., Inoue,K., Hyodo,S. and Takei,Y.
  TITLE     A novel guanylin family (guanylin, uroguanylin, and renoguanylin)
            in eels: possible osmoregulatory hormones in intestine and kidney
  JOURNAL   J Biol Chem 278 (25), 22726-22733 (2003)
   PUBMED   12684514
REFERENCE   8  (bases 1 to 602)
  AUTHORS   Hidaka,Y., Shimono,C., Ohno,M., Okumura,N., Adermann,K.,
            Forssmann,W.G. and Shimonishi,Y.
  TITLE     Dual function of the propeptide of prouroguanylin in the folding of
            the mature peptide: disulfide-coupled folding and dimerization
  JOURNAL   J Biol Chem 275 (33), 25155-25162 (2000)
   PUBMED   10827170
REFERENCE   9  (bases 1 to 602)
  AUTHORS   Whitaker,T.L., Steinbrecher,K.A., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A. and Cohen,M.B.
  TITLE     The uroguanylin gene (Guca1b) is linked to guanylin (Guca2) on
            mouse chromosome 4
  JOURNAL   Genomics 45 (2), 348-354 (1997)
   PUBMED   9344659
  REMARK    Erratum:[Genomics 2000 May 15;66(1):122]
REFERENCE   10 (bases 1 to 602)
  AUTHORS   Whitaker,T.L., Witte,D.P., Scott,M.C. and Cohen,M.B.
  TITLE     Uroguanylin and guanylin: distinct but overlapping patterns of
            messenger RNA expression in mouse intestine
  JOURNAL   Gastroenterology 113 (3), 1000-1006 (1997)
   PUBMED   9287995
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL645563.12, AK002364.1 and
            AV003693.1.
            
            On Nov 14, 2007 this sequence version replaced NM_008191.1.
            
            Summary: This gene encodes a member of the guanylin family and
            preproprotein that is proteolytically processed to generate a
            mature protein product. The mature protein product, known as
            uroguanylin, is an endogenous ligand for the guanylate cyclase-C
            receptor and may regulate salt and water homeostasis in the
            intestine and kidneys. Homozygous knockout mice for this gene
            exhibit impaired sodium chloride excretion and elevated arterial
            pressure. This gene is present in a gene cluster with a related
            guanylin family member on chromosome 4. [provided by RefSeq, Sep
            2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U90727.1, BG969327.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AL645563.12        3224-3252
            30-421              AK002364.1         2-393
            422-425             AV003693.1         54-57
            426-596             AK002364.1         398-568
            597-602             AL645563.12        5563-5568
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 55.48 cM"
     gene            1..602
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /note="guanylate cyclase activator 2b"
                     /db_xref="GeneID:14916"
                     /db_xref="MGI:MGI:1270851"
     exon            1..165
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /inference="alignment:Splign:2.1.0"
     CDS             91..411
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /note="uroguanylin; granule cell antiserum positive 2;
                     guanylate cyclase activator 2b (retina)"
                     /codon_start=1
                     /product="guanylate cyclase activator 2B preproprotein"
                     /protein_id="NP_032217.2"
                     /db_xref="CCDS:CCDS18587.1"
                     /db_xref="GeneID:14916"
                     /db_xref="MGI:MGI:1270851"
                     /translation="MSRSQLWAAVVLLLLLQSAQGVYIKYHGFQVQLESVKKLNELEE
                     KEMSNPQPRRSGLLPAVCHNPALPLDLQPVCASQEAASTFKALRTIATDECELCINVA
                     CTGC"
     exon            166..352
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /inference="alignment:Splign:2.1.0"
     exon            353..602
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /inference="alignment:Splign:2.1.0"
     regulatory      580..585
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /note="hexamer: AATAAA"
     polyA_site      602
                     /gene="Guca2b"
                     /gene_synonym="Gcap2; Ugn"
                     /note="major polyA site"
ORIGIN      
        1 tataaggggc tgctggccca gaggcaggtg gcaggcaggt ggacagcaga ggaagcagga
       61 acccagaggt gtgagcttgg aagcgaggcc atgtcaagaa gccaactgtg ggctgccgtc
      121 gtcctgctgc tgctcctgca gagtgcacag ggtgtctaca tcaagtacca tggcttccaa
      181 gtccagctgg aatcagtgaa gaagctgaat gagttggagg agaaggagat gtccaatccc
      241 cagcctcgga gaagtggcct cctccctgct gtgtgccata acccagcctt gcccttggac
      301 ctccagcctg tttgtgcctc ccaggaagct gccagcacct tcaaggcctt gaggaccatc
      361 gccaccgacg aatgtgaact gtgtataaat gttgcctgta caggctgctg atgaaatgac
      421 tctgggtctt aagaccactc cggacacttt ccccacagcc caacctgtcc atacttaggc
      481 accattgaag taatcgccat cctcccagca taaatggatc cttagcaagg caatgtggat
      541 gcagagttgc catatttggc ccccaggcag ctgcacctga ataaaaaatg ctacccacaa
      601 aa
//
