LOCUS       NM_008190                560 bp    mRNA    linear   ROD 26-JUN-2024
DEFINITION  Mus musculus guanylate cyclase activator 2a (guanylin) (Guca2a),
            mRNA.
ACCESSION   NM_008190
VERSION     NM_008190.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 560)
  AUTHORS   Pattison,A.M., Barton,J.R., Entezari,A.A., Zalewski,A.,
            Rappaport,J.A., Snook,A.E. and Waldman,S.A.
  TITLE     Silencing the intestinal GUCY2C tumor suppressor axis requires APC
            loss of heterozygosity
  JOURNAL   Cancer Biol Ther 21 (9), 799-805 (2020)
   PUBMED   32594830
REFERENCE   3  (bases 1 to 560)
  AUTHORS   Srivillibhuthur,M., Warder,B.N., Toke,N.H., Shah,P.P., Feng,Q.,
            Gao,N., Bonder,E.M. and Verzi,M.P.
  TITLE     TFAM is required for maturation of the fetal and adult intestinal
            epithelium
  JOURNAL   Dev Biol 439 (2), 92-101 (2018)
   PUBMED   29684311
REFERENCE   4  (bases 1 to 560)
  AUTHORS   Fernandez-Cachon,M.L., Pedersen,S.L., Rigbolt,K.T., Zhang,C.,
            Fabricius,K., Hansen,H.H., Elster,L., Fink,L.N., Schafer,M.,
            Rhee,N.A., Langholz,E., Wandall,E., Friis,S.U., Vilmann,P.,
            Kristiansen,V.B., Schmidt,C., Schreiter,K., Breitschopf,K.,
            Hubschle,T., Jorsal,T., Vilsboll,T., Schmidt,T., Theis,S.,
            Knop,F.K., Larsen,P.J. and Jelsing,J.
  TITLE     Guanylin and uroguanylin mRNA expression is increased following
            Roux-en-Y gastric bypass, but guanylins do not play a significant
            role in body weight regulation and glycemic control
  JOURNAL   Peptides 101, 32-43 (2018)
   PUBMED   29289697
  REMARK    GeneRIF: Guanylin and uroguanylin, as well as their prohormones, do
            not seem to play a significant role in body weight regulation and
            glycemic control, suggesting that guanylin-family peptides do not
            show promise as targets for the treatment of obesity or diabetes.
REFERENCE   5  (bases 1 to 560)
  AUTHORS   Ikpa,P.T., Sleddens,H.F., Steinbrecher,K.A., Peppelenbosch,M.P., de
            Jonge,H.R., Smits,R. and Bijvelds,M.J.
  TITLE     Guanylin and uroguanylin are produced by mouse intestinal
            epithelial cells of columnar and secretory lineage
  JOURNAL   Histochem Cell Biol 146 (4), 445-455 (2016)
   PUBMED   27246004
  REMARK    GeneRIF: guanylin and uroguanylin are produced by mouse intestinal
            epithelial cells of columnar and secretory lineage
REFERENCE   6  (bases 1 to 560)
  AUTHORS   Whitaker,T.L., Steinbrecher,K.A., Copeland,N.G., Gilbert,D.J.,
            Jenkins,N.A. and Cohen,M.B.
  TITLE     The uroguanylin gene (Guca1b) is linked to guanylin (Guca2) on
            mouse chromosome 4
  JOURNAL   Genomics 45 (2), 348-354 (1997)
   PUBMED   9344659
  REMARK    Erratum:[Genomics 2000 May 15;66(1):122]
REFERENCE   7  (bases 1 to 560)
  AUTHORS   Sciaky,D., Jenkins,N.A., Gilbert,D.J., Copeland,N.G., Sonoda,G.,
            Testa,J.R. and Cohen,M.B.
  TITLE     Mapping of guanylin to murine chromosome 4 and human chromosome
            1p34-p35
  JOURNAL   Genomics 26 (2), 427-429 (1995)
   PUBMED   7601480
REFERENCE   8  (bases 1 to 560)
  AUTHORS   Hill,O., Kuhn,M., Zucht,H.D., Cetin,Y., Kulaksiz,H., Adermann,K.,
            Klock,G., Rechkemmer,G., Forssmann,W.G. and Magert,H.J.
  TITLE     Analysis of the human guanylin gene and the processing and cellular
            localization of the peptide
  JOURNAL   Proc Natl Acad Sci U S A 92 (6), 2046-2050 (1995)
   PUBMED   7892222
REFERENCE   9  (bases 1 to 560)
  AUTHORS   Sciaky,D., Kosiba,J.L. and Cohen,M.B.
  TITLE     Genomic sequence of the murine guanylin gene
  JOURNAL   Genomics 24 (3), 583-587 (1994)
   PUBMED   7713512
REFERENCE   10 (bases 1 to 560)
  AUTHORS   de Sauvage,F.J., Keshav,S., Kuang,W.J., Gillett,N., Henzel,W. and
            Goeddel,D.V.
  TITLE     Precursor structure, expression, and tissue distribution of human
            guanylin
  JOURNAL   Proc Natl Acad Sci U S A 89 (19), 9089-9093 (1992)
   PUBMED   1409606
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from M95175.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M95175.1, BF579252.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 55.47 cM"
     gene            1..560
                     /gene="Guca2a"
                     /gene_synonym="Guca2"
                     /note="guanylate cyclase activator 2a (guanylin)"
                     /db_xref="GeneID:14915"
                     /db_xref="MGI:MGI:102738"
     exon            1..94
                     /gene="Guca2a"
                     /gene_synonym="Guca2"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..370
                     /gene="Guca2a"
                     /gene_synonym="Guca2"
                     /note="guanylate cyclase activator 2 (guanylin 2,
                     intestinal, heatstable)"
                     /codon_start=1
                     /product="guanylin precursor"
                     /protein_id="NP_032216.1"
                     /db_xref="CCDS:CCDS18586.1"
                     /db_xref="GeneID:14915"
                     /db_xref="MGI:MGI:102738"
                     /translation="MNACVLSVLCLLGALAVLVEGVTVQDGDLSFPLESVKKLKGLRE
                     VQEPRLVSHKKFAPRLLQPVAPQLCSSHSALPEALRPVCEKPNAEEILQRLEAIAQDP
                     NTCEICAYAACTGC"
     exon            95..305
                     /gene="Guca2a"
                     /gene_synonym="Guca2"
                     /inference="alignment:Splign:2.1.0"
     exon            306..560
                     /gene="Guca2a"
                     /gene_synonym="Guca2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      560
                     /gene="Guca2a"
                     /gene_synonym="Guca2"
ORIGIN      
        1 tgcattgcat actgctacca tgaatgcctg tgtgctctct gtgctgtgcc tcctgggtgc
       61 cttggctgtc ctggtagaag gggtcactgt gcaggatgga gacctctcct ttcctctgga
      121 gtcagtgaaa aaacttaagg gtctccgcga ggtacaggag cccagactgg tgagtcacaa
      181 gaagtttgct ccccggcttc ttcagcccgt ggcaccacag ctatgtagta gccactctgc
      241 acttccagaa gcactgaggc cggtctgcga gaaacccaac gccgaggaga tcctgcagag
      301 gctagaggcc attgctcagg acccaaacac atgtgagatc tgtgcctacg ctgcctgtac
      361 gggatgctag agtgacatcg cttgcctttc tctcagccca tgtggaagcc cctctatctt
      421 ctagaagtca cgaggctaac aaactcatac cctcagcatc tattgccctg ccacagcggg
      481 gaggaggctg aggagacagc tacctggaga agccttgcct gagggctatg ttaaccctca
      541 gccaataaac caaacttcag
//
