LOCUS       NM_027000               9072 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus GTP binding protein 4 (Gtpbp4), transcript variant 1,
            mRNA.
ACCESSION   NM_027000
VERSION     NM_027000.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 9072)
  AUTHORS   Xue,Q., Zhu,Z., Xue,Z., Yang,F., Cao,W., Liu,X., Liu,H. and
            Zheng,H.
  TITLE     NOG1 downregulates type I interferon production by targeting
            phosphorylated interferon regulatory factor 3
  JOURNAL   PLoS Pathog 19 (7), e1011511 (2023)
   PUBMED   37410776
  REMARK    GeneRIF: NOG1 downregulates type I interferon production by
            targeting phosphorylated interferon regulatory factor 3.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9072)
  AUTHORS   Wang,M., Anikin,L. and Pestov,D.G.
  TITLE     Two orthogonal cleavages separate subunit RNAs in mouse ribosome
            biogenesis
  JOURNAL   Nucleic Acids Res 42 (17), 11180-11191 (2014)
   PUBMED   25190460
REFERENCE   4  (bases 1 to 9072)
  AUTHORS   Lapik,Y.R., Misra,J.M., Lau,L.F. and Pestov,D.G.
  TITLE     Restricting conformational flexibility of the switch II region
            creates a dominant-inhibitory phenotype in Obg GTPase Nog1
  JOURNAL   Mol Cell Biol 27 (21), 7735-7744 (2007)
   PUBMED   17785438
  REMARK    GeneRIF: These results suggest that conformational changes in the
            switch II element of Nog1 have a critical importance for the
            dissociation of preribosome-bound factors during intranucleolar
            maturation.
REFERENCE   5  (bases 1 to 9072)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   6  (bases 1 to 9072)
  AUTHORS   Couture,M., Burmester,T., Hankeln,T. and Rousseau,D.L.
  TITLE     The heme environment of mouse neuroglobin. Evidence for the
            presence of two conformations of the heme pocket
  JOURNAL   J Biol Chem 276 (39), 36377-36382 (2001)
   PUBMED   11473111
  REMARK    GeneRIF: Resonance Raman spectroscopy was used to determine the
            structure of the heme environment in Ngb from mice.
REFERENCE   7  (bases 1 to 9072)
  AUTHORS   Laping,N.J., Olson,B.A. and Zhu,Y.
  TITLE     Identification of a novel nuclear guanosine triphosphate-binding
            protein differentially expressed in renal disease
  JOURNAL   J Am Soc Nephrol 12 (5), 883-890 (2001)
   PUBMED   11316846
REFERENCE   8  (bases 1 to 9072)
  AUTHORS   Burmester,T., Weich,B., Reinhardt,S. and Hankeln,T.
  TITLE     A vertebrate globin expressed in the brain
  JOURNAL   Nature 407 (6803), 520-523 (2000)
   PUBMED   11029004
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC125484.4 and AC124732.5.
            
            On Mar 22, 2023 this sequence version replaced NM_027000.4.
            
            Sequence Note:.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK166711.1, BC025431.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-96                AC125484.4         45761-45856         c
            97-267              AC125484.4         42457-42627         c
            268-371             AC125484.4         41744-41847         c
            372-508             AC125484.4         41527-41663         c
            509-609             AC125484.4         40510-40610         c
            610-702             AC125484.4         38865-38957         c
            703-894             AC125484.4         37568-37759         c
            895-960             AC125484.4         37061-37126         c
            961-1050            AC125484.4         35450-35539         c
            1051-1161           AC125484.4         35021-35131         c
            1162-1239           AC125484.4         29714-29791         c
            1240-1291           AC124732.5         218-269
            1292-1392           AC124732.5         1070-1170
            1393-1590           AC124732.5         2265-2462
            1591-1656           AC124732.5         4688-4753
            1657-1800           AC124732.5         5391-5534
            1801-9072           AC124732.5         6424-13695
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 3.64 cM"
     gene            1..9072
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="GTP binding protein 4"
                     /db_xref="GeneID:69237"
                     /db_xref="MGI:MGI:1916487"
     exon            1..96
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     CDS             49..1953
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="isoform 1 is encoded by transcript variant 1; GTP
                     binding protein 3; chronic renal failure gene protein;
                     Nucleolar GTP-binding protein 1 (Chronic renal failure
                     gene protein) (GTP-binding protein NGB); chronic renal
                     failure protein"
                     /codon_start=1
                     /product="GTP-binding protein 4 isoform 1"
                     /protein_id="NP_081276.2"
                     /db_xref="CCDS:CCDS26236.1"
                     /db_xref="GeneID:69237"
                     /db_xref="MGI:MGI:1916487"
                     /translation="MAHYNFKKITVVPSAKDFIDLTLSKTQRKTPTVIHKHYQIHRIR
                     HFYMRKVKFTQQNYHDRLSQILSDFPKLDDIHPFYADLMNILYDKDHYKLALGQINIA
                     KNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTIIKRQRQSLEYLEQVRQHLSR
                     LPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHMDYKYLRWQ
                     VVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLKEQLELFQNIR
                     PLFINKPLIVVANKCDVKRIAELSEEDQKIFLDLQAEGFPVIETSTLTEEGVIQVKTE
                     ACDRLLAHRVETKMKGNKVNEVLNRLHLAVPNKRDDKERPPFIPEGVIARRKRMEIEE
                     PKKKRERDLELEMGDDYILDLQKYWDLMNSSEKYDKIPEIWEGHNVADYIDPAIMKKL
                     EELEKEEELRTAAGEYDSDSESEDEEMMEIRQLAKQIREKKKLKILQSKEKNKQGPRM
                     PRTAKKVQRADLENEMRSLGVDMDDKNNAHYAVQARRSRSVTRKRKREESVPPSSTAR
                     SRSCSRTPRDVSGLRDVKMVKKAKTMMKKAQKKMNRLGKKGEADRHVFDMKPKHLLSG
                     KRKAGKKDRR"
     exon            97..267
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            268..371
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            372..508
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            509..609
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            610..702
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            703..894
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            895..960
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            961..1050
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1161
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1239
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1240..1291
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1392
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..1590
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1591..1656
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1657..1800
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..9072
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2428..2433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="hexamer: AGTAAA"
     polyA_site      2450
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="major polyA site"
     regulatory      2983..2988
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="hexamer: ATTAAA"
     polyA_site      3005
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
     regulatory      3020..3025
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="hexamer: ATTAAA"
     polyA_site      3045
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
     regulatory      5451..5456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="hexamer: AATACA"
     polyA_site      5478
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
     regulatory      9051..9056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
                     /note="hexamer: AATAAA"
     polyA_site      9072
                     /gene="Gtpbp4"
                     /gene_synonym="2610028C09Rik; Crfg; Gtpbp3; NGB; Nog1"
ORIGIN      
        1 agtccggtcc tccgcttctc ctggttcctc gtgcgttcca ccgccggcat ggcgcattac
       61 aacttcaaga agattacggt ggtgccgtct gccaaggact ttattgacct aacattatcc
      121 aagactcaac gaaagactcc aacggttatt cataaacatt atcaaattca tcggattcga
      181 catttttata tgagaaaagt caaatttact caacagaatt accatgacag actgtcacag
      241 attctgtcgg atttccccaa attggatgat atccatccgt tttatgctga cttgatgaat
      301 attctctatg acaaggatca ctacaagttg gctctaggtc aaataaatat tgccaaaaac
      361 ttggtggaca atgttgctaa agattatgtg cgtcttatga aatatggtga ttctctgtac
      421 cgatgcaagc agctgaagcg cgcggccctt ggacgaatgt gtactattat caagagacag
      481 aggcagagct tggagtatct ggaacaagta cgtcaacatt tatcccgctt gccaaccatt
      541 gatccaaata caaggactct gcttttgtgt gggtatccaa atgttgggaa atccagcttc
      601 atcaacaagg tgacaagagc agatgtggac gttcaaccct atgcatttac caccaaatct
      661 ctgtttgttg ggcacatgga ttataagtat ctgcgctggc aggttgtaga taccccaggg
      721 attttggatc atcctttgga ggatcggaac accattgaga tgcaggccat cacagccctg
      781 gcccacctcc gagctgcagt tctgtatgtg atggatctgt ctgagcagtg tggacatggg
      841 ttaaaggagc agttagaact cttccagaat atcagacctc tgtttatcaa caagcctctt
      901 attgttgtag caaacaaatg tgatgtgaag agaatagctg aactttctga agaagatcag
      961 aaaatatttc tagacttgca ggctgaagga ttccctgtca tcgagaccag taccctgact
     1021 gaggaaggtg tcattcaagt taaaacagag gcgtgtgaca ggcttttggc tcaccgagta
     1081 gaaacgaaaa tgaaaggaaa taaagtgaat gaggttctga acagattgca tttagctgtg
     1141 cccaacaaga gagatgataa ggagagaccc cctttcatcc cagaaggagt gatagctcga
     1201 aggaagagaa tggagattga agagcccaag aagaagaggg agcgagatct tgagctggaa
     1261 atgggagatg attacatttt ggatcttcag aaatactggg acttaatgaa ttcatctgag
     1321 aaatatgata agataccaga aatttgggaa ggccacaatg ttgctgatta tattgatcct
     1381 gctatcatga agaaattaga agagttggaa aaggaagaag agctaagaac agctgctggg
     1441 gagtatgaca gtgactctga gagtgaagac gaggaaatga tggaaatccg acagctggca
     1501 aagcaaatta gagaaaagaa gaagttgaaa attctacagt ccaaagaaaa gaataaacag
     1561 ggacccagga tgccccgaac tgccaagaag gttcaacggg cagatttaga gaatgagatg
     1621 cgtagtcttg gtgtggacat ggatgataaa aacaatgccc actatgcagt ccaggcaaga
     1681 aggtctcgga gtgtcacgag gaaaagaaaa cgagaagaat ctgttccacc ctcttccaca
     1741 gcgcggagtc gcagttgttc tagaactcca cgtgatgttt ctggtcttcg ggatgtcaag
     1801 atggtgaaga aagccaagac tatgatgaag aaagctcaga agaagatgaa tcgcttgggg
     1861 aagaaagggg aagctgatag gcatgtgttt gatatgaagc ccaagcactt gctgtctggg
     1921 aagaggaaag ctggtaaaaa ggacaggaga taatgtcctc ttcagttgga acagtttaag
     1981 tagactcttg gttattttgg gtgtggtatg gtgtgttata gaatttggaa tcaccatctg
     2041 ttgaccaaaa gaaacagtgg gaatgacttt taaatgcttg ctgctttgct ggaaactggc
     2101 aaaccctact aagtgtgcag tgctgtgctc actgcgcagt ttgggttttt atgtccagtg
     2161 cgttccgtac ttgatgagct tctttcagca ttttacagaa aagacttatt ggtgtacgga
     2221 ggctgcgtgg gtggaggaaa actgttttgt agtgggactt ttgtcaccta aagtacctat
     2281 ttttgttaag aaagtatacc tgctgggagg tctaggcaga aggctatcct ccttacattt
     2341 agggtttatc agagcttaaa ctactcttaa attattttct aaacctgtag tacaatgtat
     2401 cattgtaact tcaaccattt tatccaaagt aaacacccag caaaaacata gtttgatgac
     2461 ttacattttg tttgatttaa ttgcaataca cttgatgacc aaaaggggtg gtggtggcag
     2521 ggaataggac tttgcttttt tgttcttaga ataaaaaata aaggaagtaa gtgcctgggg
     2581 tataggctca aaaccaaaaa aaaaaaaaaa agaagaaaga aagaaagaaa gaaagaaaga
     2641 aagaaagaaa gaaagaaaga aaatgtcttc tttcataaac acttgctgaa ttaagattga
     2701 tgtcctggaa agtacaaaaa catctacaag cgggatcccg gtgtacctgg aagtgcagcc
     2761 ctgtcctcag attagaggat aatcttcaga cagtcagtgc ttcctgttag gctgcagctt
     2821 ctggccttta tgagaatcca gcgactaccg acggattacg tactctagcc taagattctt
     2881 tggactctat tgagacctct tttttaaatt ggctttctaa tgtgttttag ttctctgaag
     2941 aagtttgatg tcttgatctt tttttttttt aatgtctaga tgattaaagt ctataatcat
     3001 ttctatgcct agtaacttaa ttaaatcata gaattagtca tgtaacagaa gttctagaca
     3061 aggctttttt cttaatggaa cttgtattta ttaaaatgtc tgagacatta gtgagaaagg
     3121 gtagagagac tggaaaaatt aatgagagga ggaaaacaag gcttagctag agtttgtatt
     3181 tcccttggca gaagtcagga aaattcaaca taaattatga aaccaagatt gttgacatac
     3241 ataatactgg tttttctaaa gacatctcag ctgttcaata caaaagaatt aagcatattt
     3301 aggaacagaa tgtttgtgac cttgagctga actgtgttag gcgtcagttc tcattgctgt
     3361 taggactagg aagaggtggc tgtgaatgct ggcttggtac gtaggctgcc tttctgccta
     3421 ccaggtcctt tagcggtgct ggttgctagc aggagaagca ggggtgttgt gaaggttcag
     3481 cagcgtcccc tccagagaga cactaacaag gaaaatgtta gaggacccat tctgattgta
     3541 agcatacaca acaaggtaat agtaataaca ttgtgatact gtcataatga cagccataca
     3601 gactgttgga acaaagtaga caactcagaa ataggtatac taaaaaatgg actggaacta
     3661 accgtgggca aaggactctt tctccagcag ccattttggg aaatgttaac atatatggtc
     3721 aaaagttgga ctctacaccc cactagatac ataaattttt aaaagcctaa gtataagatc
     3781 cctaaaacta caacgtttta aagaaaatgc taaaactatc ataacattgg atttgacaat
     3841 ttcttggctg tgacatcaga gttataggca ggaaaaaaga aagtacataa acaggactcg
     3901 attagaattc tgtaggagac tgcctacaga atgggagagc aggtttgcaa gtgtgagtaa
     3961 tacatggttt gtaaattcaa cagtgaccag ctcaagtatc ggcaaaagtc ttgaatatct
     4021 atttttccaa acaagatagt caagtgctca gatagccata cgaaagcatc tggggttcac
     4081 ctaatcgtta gggagagtca gcgagaagca cgactgagca ccaagacggt cagatcaaac
     4141 aacagccaag aaggggcaag cacagaaagc caagcaaatc cttcccttgt agttcttccc
     4201 tcggatgttt ggtacagtgc ttacctaaca tggaacacac atggggatag acagtcaaca
     4261 cccaggtggc tgtgagaacg ctcaccatgc ctagctgctt atcctgcttg atactcagat
     4321 aagtatcctc ccttgagaaa cataactaac catattgtaa aaactacctc cttcgttttt
     4381 agttcttttc taaagatagc agtcaatctt gtttgtaaga gtgatgatat catgtttatt
     4441 ctattcctgg ggtttaatac ataaaaagaa aaatgtttat aaccagaaaa tgtaattatt
     4501 tttaactagg ttactctttg ttcactagat ttagattttt ctctagatct gtctgtgata
     4561 atatcactaa tacagtgatc tgaagttagg gtctatttcc atcaaggtaa aaaccattgc
     4621 tcagccaggg ggtggtggcc cacgtcttta atcccagcac ttgggaggca gaggcaggtg
     4681 gatttctgag ttcgaggcca gcctggtcta cagagtgagt tccaggacag ctagggctac
     4741 acagaaaaac cctgtctcaa ataaacagaa aacaatacca aaccattgtt caccgtgggg
     4801 tgggtgtgat gtttattgtt tgttgactac atggggaatt aacaaaaacc caaattggag
     4861 agcacataga gctgtgaggg atttttgctt aatttgaagt tggaaggtgc taggttttga
     4921 ggtcagaaga cacaccttta atccagatct tttgataagg gaagacataa cctctaatta
     4981 gggttgcacc ttctgctgga agcctatata gggacataga aggaggagtc ttttgctctt
     5041 tgcctacacc agcaagtcca ttccttcatt agcattagag ccagcttttt ttttttcata
     5101 taccaatgtt ttcttttaaa atgtttattt ttgccgctaa ttaagtatac gtctgcgtgt
     5161 ctattgtagg gtatgcgcat gtgaatgaag atatctgcat aggagtcaga ggtgttgggt
     5221 tgtagctgaa actacaggtc cttgtgaaac acccaataat ggttctggga attgaactca
     5281 ggtcctttgc aagtgcagca tacattctta actcctgagc catctagagc cagcttcttt
     5341 aggattctag catataccaa agaccaagtg agaaatccaa tctcatagac tgaacaatta
     5401 ctgaggtctt gtaccttctg tacataggca gccattattg gattatctgg aatacagcgt
     5461 gtaaatcatt ctaatgaatc ctgtgtgtgt gtgtgaagag aatcattctt taagctctgt
     5521 tactttagag gtccctgact aaggcaggtg ggaacattac taacatggtc ttagcaactg
     5581 cacacatctt gtctattcca cataaggatg gaggatgcga ggtaagtgac ctcaacactg
     5641 cacggaagct atttaggggg aaacaaagat ttgcaatcag ccttagaagg tcagagatga
     5701 cagaaaaaag tcttggattc ttcctgggaa ttccagtgca aaaattgctg aatttaaatg
     5761 ttgcagcaga acaggggtga tggcataaaa gacatcctgg agtttcaatt tttttttttt
     5821 aatttttcaa gaaaattgtc taagcttggt gtaaaagctt ggcacatggt tttgattcca
     5881 acacctggga ggcagaagta gatggatttt tgagtgggat gctagcctgg tctacagtga
     5941 attccaggac agtcagagct atatagtaag gccctgtatt ggcaaaaaaa aaagaaagaa
     6001 aagaaacata actaataaaa ttactggtag caaggagtct aagaagcaat gaaatagaag
     6061 gctgattttg ttttctcttc actagaaatc agtgaggtaa actgaccttt tccaggtttt
     6121 ttgatgactg aaccttttag cacaattctc atagtaaaga ttatgacctt tggtcattat
     6181 tgataaatat attcacaata acatttgacc aagcaaatgt atcccttgat ccacaccaac
     6241 gaacgttaaa ttgtttgact ttgccactgt tttatgcata gaagatagac ttaatgcttg
     6301 gtgctggggc tcagcgctgg cttaggtaaa aatacagcag gaagtacttg attatgctca
     6361 tcagctccac aaagagtcct tgtggaatct gtgaacccag tgaaaaccaa ggaagctctt
     6421 tgttatccat atccatatcc atcctcactc accatatgcc acctggagag agaaccttgg
     6481 acaggtgagc ctgtgttacc catgggagag tggtgtcacc aggggaaggt tttaggagaa
     6541 aactgacact ttccatagat gatattgaaa aaatttaaga gctggagaga gagaagcaat
     6601 taaaatgaag agcttgaatt ttagtttagt gcaattaaag gaaattagta ttcaagtttc
     6661 tgttaaacat gacagtttta accatgggtt agtaatgaac ccaacttttt ctttcttttt
     6721 ttttttttta agatatttta aatttttatg tgtatgggtt atttgcctgg atctgtatgt
     6781 ataccacata atgcgtgcca ggtacccaca gaggtcataa gaggggtaag atcccctgga
     6841 actagagtta tagtagtgag ccacaatatg agtgctggaa tggaaccccg gactcatgca
     6901 ggagcatcgt agttactgag tcatttttca accccattcc ctgcttcgaa cattactttt
     6961 tatatccctc agagtcgata tatcttttca cacacatcct caaaataagg ccatcaaggg
     7021 aacaggaagt cctctaccat gcttctgaac tgtgtggcga tctcttctca caggcctccc
     7081 tgtccagtag ctcctgcacg tcccagctca tatacaccac ttccctggta tctgggttta
     7141 gcataaggta ttttctcacc aacagaaagt agccaatctc atgatctccc cagaccgcat
     7201 ctggctggca cttgtggtat tttcgggtca ttaagattat ttcctttatg aaaattgttg
     7261 gagttcgtca aaagaccctc actcacaaag accacagaga ttgccatcgc tgcaaaccac
     7321 atgaggattt ttattgatcc aacatgttgg ggactcccct ctcagcatgc aggccagagg
     7381 agagacccag aacaaagaac acacttatat cttgttatgg tgatttctct gaaaacagct
     7441 aatattattt tggcagttgc aagcttagtc attttgactg ctaaaacttt ttacatttgt
     7501 ttagccagct tctttatctg tctctgtact ttgcctgcta gtacagtttg gaaagtcctt
     7561 cttgaagggg gaaggtactg ggtggtcttg aatttatttt ggatattaaa aagtatattt
     7621 ggatattaag aagttggaac tgtattaatt cagtaataga gaaactggac atttagatag
     7681 aaggcagctc attttttttc ccctcattcc cttcaagggt gccacacaag cacttaagct
     7741 taattagatg ataggtagat cacagtgaag agggcactga ccctcttggg tttcatttcc
     7801 tggccactat ggtttccttt ttcatttaca atgaagacca tgtatataca ggatgcaaac
     7861 ccagttatgt acaataggac atacaagtat gtaaaagatt tcagaatgga aatgaaaaca
     7921 ctggagggct gatgatggtt gcacatgcct ttaatcacat acttctgagg cagaggtagg
     7981 cagataactg tgagggtgag gttcacctct acaaagcaag ttccgggaca gccaggctta
     8041 cttacacaca cacctcctat gaaaaacagc aatcaccact caagctccag tacagtggct
     8101 tgtttcacag caagtcctgt ggaccaggat tgggcacttc cacaggaaag ctgtttccta
     8161 tgcaagacag agccatgtta gccctgcagc tacttcctga gttagcattt tggtggatga
     8221 aactagacag cactgtcata tgacattatt cctattaaaa gatccctgag cacatggaga
     8281 ggtacactga gccccatgct ggagtgagtg ggtacctggt catgagagat gcccagctca
     8341 gcctgcagac gtctcaggac tgcactcctc actcatttga acgggtgttg ggaacttaac
     8401 tgagatactc tggaagagca gtgaacactc ttaattgctg aactatctcc cctagcctct
     8461 aacatgtcca catttaaata ttaaaagttg gcagtggctc attatctcta cgtgactcta
     8521 ctaacaataa atatgtagtg agtggatgta tgtactaaaa cattgtgggg ttgtagatct
     8581 taaatttaac tagaaactcc agattgcctg acaatcatgc tgctggttct tcacttcctt
     8641 gcttttattt tatactcata cacacacaca cagcactctg taatcccgca ctatttgcaa
     8701 aaagtggaac atatttgtag aaaaattaaa aatattttgt atggtgtgct gttgagcatt
     8761 cttgtcaact tcttcttgga gaagaaggaa cttcagttga gaaaatggct ccataataag
     8821 atctgtagga catttttaaa atgagtgatt aatggggagg gcccaaccca ttgtgagttg
     8881 aactactgct gggctggtgg tcctgggtag cataagaaag caggctgagc aagccatgag
     8941 gagcaagcca gtaagcagca cccctccatg acttgtgcat cagctcctgc ctcccaagtt
     9001 cctgccctga tttccttcag taatgaaaag tggttcagaa gcgtaagcca aataaaccct
     9061 ttcctccctg aa
//
