LOCUS       NM_032544               2053 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus GTP binding protein 3 (Gtpbp3), transcript variant 1,
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_032544
VERSION     NM_032544.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Li,X. and Guan,M.X.
  TITLE     Identification and characterization of mouse GTPBP3 gene encoding a
            mitochondrial GTP-binding protein involved in tRNA modification
  JOURNAL   Biochem Biophys Res Commun 312 (3), 747-754 (2003)
   PUBMED   14680828
  REMARK    GeneRIF: These observations suggest that the mouse Gtpbp3 is an
            evolutionarily conserved mitochondrial GTP-binding protein involved
            in the tRNA modification.
REFERENCE   3  (bases 1 to 2053)
  AUTHORS   Li,X. and Guan,M.X.
  TITLE     A human mitochondrial GTP binding protein related to tRNA
            modification may modulate phenotypic expression of the
            deafness-associated mitochondrial 12S rRNA mutation
  JOURNAL   Mol Cell Biol 22 (21), 7701-7711 (2002)
   PUBMED   12370316
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC127416.7.
            
            On Jun 15, 2018 this sequence version replaced NM_032544.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF361482.2, BC138560.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-113               AC127416.7         151322-151434
            114-361             AC127416.7         151665-151912
            362-448             AC127416.7         152022-152108
            449-651             AC127416.7         152929-153131
            652-724             AC127416.7         153489-153561
            725-868             AC127416.7         153660-153803
            869-1034            AC127416.7         154019-154184
            1035-1313           AC127416.7         154671-154949
            1314-2053           AC127416.7         155133-155872
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 34.43 cM"
     gene            1..2053
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /note="GTP binding protein 3"
                     /db_xref="GeneID:70359"
                     /db_xref="MGI:MGI:1917609"
     exon            1..113
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             61..1539
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /note="tRNA modification GTPase GTPBP3, mitochondrial"
                     /codon_start=1
                     /product="tRNA modification GTPase GTPBP3, mitochondrial
                     precursor"
                     /protein_id="NP_115933.2"
                     /db_xref="CCDS:CCDS40384.1"
                     /db_xref="GeneID:70359"
                     /db_xref="MGI:MGI:1917609"
                     /translation="MWRGLSALVTQAAWAPLRLCARCSTSAESLVPSSTIFALSSGQG
                     RCAIAVIRTSGPASGLALRSLTALQEPPPARRACLRLLRHPCSGEPLDRSLVLWFPGP
                     QSFTGEDCVEFHVHGGPAVVSGVLQALGSVPGLRPAEAGEFTRRAFAHGKLSLTEVEG
                     LADLIRAETEAQRRQALRQLDGELSQLCQGWAKTLTKALAYVEAYIDFGEDDNLEEGV
                     LEQADREVRALEVALGSHLRDARRGQRLLSGANVVVTGPPNAGKSSLVNLLSQKPVSI
                     VSPEPGTTRDVLETPVDLAGFPVLLSDTAGLREGVGAVEQEGVRRARHRLEQADIILG
                     VLDASDLASSSSCSFLDTVVTPLLAQSQDSGGQRLLLLLNKSDLLSANAPACDIALPP
                     HLLLSCHTGAGMDSLLQALKTELAAVCGDPSTGPPLLTRVRHQYHLQGCLDALGHYQL
                     ATDLALAAEALRQARRQLNHLTGGGGTEEILDLIFQDFCVGK"
     exon            114..361
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            362..448
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            449..651
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            652..724
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            725..868
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1034
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1313
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..2053
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2030..2035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      2053
                     /gene="Gtpbp3"
                     /gene_synonym="2410009F13Rik"
                     /note="major polyA site"
ORIGIN      
        1 cttcccgaac ttgaagccct gcgggagtgt cggccaaggt gcttgcaaaa ttggaaatcc
       61 atgtggcgtg ggctgagcgc cctggtgacc caggcggcgt gggcgcccct caggctgtgc
      121 gcacgctgca gcaccagtgc agagtcctta gtccccagca gcaccatctt cgcactcagc
      181 tccggccagg gacgctgcgc catcgcagtg atccgcacca gcggtccagc tagcgggctc
      241 gcgctccgca gcctcacggc gctccaggag ccgcctcctg cccgcagagc ctgcctgcgc
      301 cttctccgcc acccgtgctc cggggagcca ctggaccgct cactcgtcct ctggttccca
      361 ggaccccaga gtttcacggg tgaggattgc gtggagtttc acgtacacgg aggtcctgcg
      421 gtggtcagcg gtgtcttgca ggcattgggt agtgtgccag gactgcggcc agccgaggcc
      481 ggagagttca ccaggagggc atttgcccat ggcaagctga gcctgactga agtggaggga
      541 ctggcagatc ttatccgtgc agaaactgag gcccagcgtc gacaggctct gaggcagctg
      601 gatggggaac tgagccagct ctgccagggc tgggcaaaga ctctcaccaa ggctctggcc
      661 tatgtggagg cctacattga cttcggagag gatgataatt tggaagaggg tgtgttggag
      721 caggcggaca gagaagttcg agccctggag gtggcgctgg gttcccacct gcgagatgct
      781 aggcgtggac aaaggctcct ctcgggggca aatgttgtgg tcactggacc tcccaatgcg
      841 ggcaagagca gtctggtgaa tctactcagc caaaagccag tgtccatcgt gtccccagag
      901 cctgggacca cccgtgacgt gctggagact cccgtggact tggctgggtt ccctgtcttg
      961 ctgagcgaca ctgcagggct ccgggaaggc gtgggcgctg ttgagcagga gggtgtgcgc
     1021 agggcccgcc acaggctgga gcaggctgac atcattctgg gggtgctgga cgcctccgac
     1081 ctggcctcct catccagctg cagcttcctg gacactgtag tgaccccact gctagcacaa
     1141 agccaagaca gcggtgggca gcgcctcctg ctgctgctca acaaatctga tctgctgtct
     1201 gctaatgccc cagcctgcga catagccctg cctcctcacc tgctcctgtc ctgccacacg
     1261 ggagccggga tggacagcct cctgcaggcc ctgaagaccg agctggctgc ggtgtgtggg
     1321 gacccatcca caggaccacc gctcttgacc cgggtcaggc accagtacca cctccagggc
     1381 tgcctggatg ccttgggcca ctaccagctg gccacagacc tggctctggc ggccgaggcc
     1441 ctgcgccagg ctcggaggca actaaatcac ctcacaggtg gaggaggaac ggaggagatc
     1501 ctggatctca tcttccagga cttctgtgtg ggcaagtgag gtccagagcc tgggactcag
     1561 cctcggagtt cccacaaggt catgatccaa gatggcaaac ctagcagagg gcaggagagc
     1621 actggcgtct gcagccctcc acagtgagga gagagacatg gatcagggat ctgacagtgg
     1681 atacttcctc acccccgatg tcgggattaa ggtcagcatc accttgccaa gtgcctgaat
     1741 gatgtagtgc tggatgtgaa acccgttgca tgttatatgc tgagccacga cagggcttct
     1801 ctgtgtagtc ctggctgtcc tggaactcac tctgtagacc aggctggcct tgaacccaga
     1861 aatctgcctg cctctgcctc ccaagtgctg ggattaaagg catttcattt ttaattacca
     1921 aaggagaatg aagagttcag gaaatccaga agcgtgtgtc cacaaggatc agtcagcctt
     1981 accttctaga actttgctga cgtgcttcta tttatggcat cgtttgccaa ttaaatacat
     2041 ttttaaattt tta
//
