LOCUS       NM_019581               2956 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus GTP binding protein 2 (Gtpbp2), transcript variant 1,
            mRNA.
ACCESSION   NM_019581
VERSION     NM_019581.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Spaulding,E.L., Hines,T.J., Bais,P., Tadenev,A.L.D., Schneider,R.,
            Jewett,D., Pattavina,B., Pratt,S.L., Morelli,K.H., Stum,M.G.,
            Hill,D.P., Gobet,C., Pipis,M., Reilly,M.M., Jennings,M.J.,
            Horvath,R., Bai,Y., Shy,M.E., Alvarez-Castelao,B., Schuman,E.M.,
            Bogdanik,L.P., Storkebaum,E. and Burgess,R.W.
  TITLE     The integrated stress response contributes to tRNA
            synthetase-associated peripheral neuropathy
  JOURNAL   Science 373 (6559), 1156-1161 (2021)
   PUBMED   34516839
REFERENCE   3  (bases 1 to 2956)
  AUTHORS   Terrey,M., Adamson,S.I., Gibson,A.L., Deng,T., Ishimura,R.,
            Chuang,J.H. and Ackerman,S.L.
  TITLE     GTPBP1 resolves paused ribosomes to maintain neuronal homeostasis
  JOURNAL   Elife 9, e62731 (2020)
   PUBMED   33186095
  REMARK    Erratum:[Elife. 2021 Jun 04;10:e70785. doi: 10.7554/eLife.70785..
            PMID: 34085931]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2956)
  AUTHORS   Kapur,M., Ganguly,A., Nagy,G., Adamson,S.I., Chuang,J.H.,
            Frankel,W.N. and Ackerman,S.L.
  TITLE     Expression of the Neuronal tRNA n-Tr20 Regulates Synaptic
            Transmission and Seizure Susceptibility
  JOURNAL   Neuron 108 (1), 193-208 (2020)
   PUBMED   32853550
REFERENCE   5  (bases 1 to 2956)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 2956)
  AUTHORS   Ishimura,R., Nagy,G., Dotu,I., Chuang,J.H. and Ackerman,S.L.
  TITLE     Activation of GCN2 kinase by ribosome stalling links translation
            elongation with translation initiation
  JOURNAL   Elife 5, e14295 (2016)
   PUBMED   27085088
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2956)
  AUTHORS   Ishimura,R., Nagy,G., Dotu,I., Zhou,H., Yang,X.L., Schimmel,P.,
            Senju,S., Nishimura,Y., Chuang,J.H. and Ackerman,S.L.
  TITLE     RNA function. Ribosome stalling induced by mutation of a
            CNS-specific tRNA causes neurodegeneration
  JOURNAL   Science 345 (6195), 455-459 (2014)
   PUBMED   25061210
  REMARK    GeneRIF: these results not only define GTPBP2 as a ribosome rescue
            factor but also unmask the disease potential of mutations in
            nuclear-encoded tRNA genes.
REFERENCE   8  (bases 1 to 2956)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   9  (bases 1 to 2956)
  AUTHORS   Watanabe,M., Yoshida,K., Hida,M., Kato,H., Uchida,K., Yamaguchi,R.,
            Tateyama,S. and Sugano,S.
  TITLE     Cloning, expression analysis, and chromosomal mapping of GTPBP2, a
            novel member of the G protein family
  JOURNAL   Gene 256 (1-2), 51-58 (2000)
   PUBMED   11054535
REFERENCE   10 (bases 1 to 2956)
  AUTHORS   Kudo,H., Senju,S., Mitsuya,H. and Nishimura,Y.
  TITLE     Mouse and human GTPBP2, newly identified members of the GP-1 family
            of GTPase
  JOURNAL   Biochem Biophys Res Commun 272 (2), 456-465 (2000)
   PUBMED   10833435
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC116739.13.
            
            On Jul 28, 2022 this sequence version replaced NM_019581.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051958.1, AF168991.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               AC116739.13        132375-132661       c
            288-314             AC116739.13        130192-130218       c
            315-499             AC116739.13        129323-129507       c
            500-608             AC116739.13        128868-128976       c
            609-806             AC116739.13        128518-128715       c
            807-981             AC116739.13        128072-128246       c
            982-1201            AC116739.13        127704-127923       c
            1202-1337           AC116739.13        127255-127390       c
            1338-1396           AC116739.13        127051-127109       c
            1397-1568           AC116739.13        126307-126478       c
            1569-1733           AC116739.13        125811-125975       c
            1734-2956           AC116739.13        124333-125555       c
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.89 cM"
     gene            1..2956
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /note="GTP binding protein 2"
                     /db_xref="GeneID:56055"
                     /db_xref="MGI:MGI:1860138"
     exon            1..287
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     CDS             102..1910
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /note="isoform 1 is encoded by transcript variant 1;
                     GTP-binding-like protein 2"
                     /codon_start=1
                     /product="GTP-binding protein 2 isoform 1"
                     /protein_id="NP_062527.2"
                     /db_xref="CCDS:CCDS50122.1"
                     /db_xref="GeneID:56055"
                     /db_xref="MGI:MGI:1860138"
                     /translation="MDSRVSELFGGCCRPGGGPAMGGNLKARGAGGSSSCGGPKGKKK
                     NGRNRGGKANNPPYLPPEAEDGNIEYKLKLVNPSQYRFEHLVTQMKWRLQEGRGEAVY
                     QIGVEDNGLLVGLAEEEMRASLKTLHRMAEKVGADITVLREREVDYDSDVPRKITEVL
                     VRKVPDNQQFLDLRVAVLGNVDSGKSTLLGVLTQGELDNGRGRARLNLFRHLHEIQSG
                     RTSSISFEILGFNSKGEVVNYSDSRTAEEICESSSKMITFIDLAGHHKYLHTTIFGLT
                     SYCPDCALLLVSANTGIAGTTREHLGLALALKVPFFIVVSKVDLCAKTTVERTVRQLE
                     RVLKQPGCHKVPMLVTSEDDAVTAAQQFAQSPNVTPIFTLSSVSGESLDLLKVFLNIL
                     PPLTNSKEQEELMQQLTEFQVDEIYTVPEVGTVVGGTLSSGICREGDQLVVGPTDDGC
                     FLELRVCSIQRNRSACRVLRAGQAATLALGDFDRALLRKGMVMVSPEMNPTICSVFEA
                     EIVLLFHATTFRRGFQVTVHVGNVRQTAVVEKIHAKDKLRTGEKAVVRFRFLKHPEYL
                     KVGAKLLFREGVTKGIGHVTDVQAITAGEAQATMGF"
     exon            288..314
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            315..499
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            500..608
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            609..806
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            807..981
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1201
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            1202..1337
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1396
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1568
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1733
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     exon            1734..2956
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2936..2941
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /note="hexamer: AATAAA"
     polyA_site      2956
                     /gene="Gtpbp2"
                     /gene_synonym="nmf205"
                     /note="major polyA site"
ORIGIN      
        1 acaccccgct ctcctccggc tggcccgact cggcggtggc agtaggggcg gcggcagtgg
       61 cgggggggag gggtcgggcg gggggctggt ggcagcggcg gatggactcg cgggtatcgg
      121 agctgttcgg cggctgctgc cggcccggag gaggcccggc catgggtgga aacctcaaag
      181 ctcggggggc cggcggtagc agcagttgcg ggggcccaaa ggggaagaag aagaacggaa
      241 ggaacagagg aggtaaagcc aacaaccctc cgtacctgcc cccagaggct gaagatggaa
      301 acatcgaata taaactgaag ctggtgaacc catctcagta ccgctttgag cacttggtga
      361 cacaaatgaa gtggcggctc caggaaggac gtggtgaggc tgtctaccag attggggtag
      421 aggacaatgg gctgctggtg gggctggctg aagaggagat gcgggcttcc ctcaagaccc
      481 tgcaccggat ggcagagaag gttggggcag acatcactgt tcttcgggag cgagaggtgg
      541 attatgacag tgacgtgccc cggaagatca ctgaggtgct agtacgaaag gtccctgaca
      601 accaacagtt cctagatctc cgtgtggccg tcctagggaa tgtggactca gggaagtcaa
      661 ccttgcttgg agtcctgacc caaggagagc tggacaacgg gcggggccgg gcccggctca
      721 accttttccg ccacctgcat gagattcagt ctggccgaac ctccagcatc agctttgaga
      781 tcctgggctt taacagcaag ggagaggtgg tgaattacag cgactcacgg actgcagaag
      841 agatctgtga gagcagctcc aagatgatca ccttcattga cctggcgggc caccataaat
      901 acctgcacac caccatcttt ggcctcacct cctactgccc cgactgtgcc ctgctcctcg
      961 tcagtgccaa cactggaatc gccggcacca cacgggaaca cctggggctg gccttggccc
     1021 tgaaagtgcc cttcttcatc gtggtcagta aagtggacct gtgtgctaag accacagtgg
     1081 agaggacagt tcgccagctg gagcgggtcc tcaagcagcc tggctgccac aaggtcccca
     1141 tgctggtcac ctctgaggat gatgctgtca ctgctgccca gcagtttgcc cagtcaccca
     1201 atgtcacccc tatatttaca ctgtccagtg tgtctggaga gagcctggac cttcttaaag
     1261 tcttcctgaa tatcctgccg cccctcacca acagcaagga gcaggaggag ctcatgcaac
     1321 agttgacaga gttccaggtg gatgaaatct acacagtccc agaggtgggg actgtggttg
     1381 gaggaacact atccagtggg atctgccgcg agggggacca gctggtggta ggcccgacag
     1441 atgatggctg tttcttggag ctgagggtat gcagcatcca gcgcaaccgc tctgcgtgtc
     1501 gtgttcttcg agcaggtcag gctgctacac tcgcccttgg agactttgac cgtgcattgc
     1561 ttcgcaaggg catggtcatg gtgagtcccg agatgaatcc caccatctgc tcagtgtttg
     1621 aggcagagat agtcctactg ttccacgcca ccacctttcg gcggggattc caggtgacag
     1681 tacatgtggg caacgtacgt caaacagcag tggtggaaaa gatccacgca aaggacaagc
     1741 tgcggacagg ggagaaggca gtggtgcgtt tccgtttcct gaaacaccca gaatacctga
     1801 aggtgggcgc caaactgctg ttccgggagg gtgtgaccaa aggcatcggt catgtcacgg
     1861 atgtacaagc catcacagca ggagaggccc aggccaccat gggcttctga gtcatccaaa
     1921 cagctctact gctgtcctac aatacatgag gtgacttccg gccatgctgc ccactgttgg
     1981 cggctctgtg tgttaatagg ctagggagag gagtgtctgc tgccgcttgc ttcctgccac
     2041 gttcttggag aggtgtcaag tttggtatgg ccagggaggg gcagtcctga gggagaagac
     2101 ggttcagggc agtgctcagc agctgtgtgc tcccctggtt gggtcattaa ccctgcagct
     2161 gcctggtctt gtctgctgga gggaggagaa cacagccatc ttggcccact atcaggacca
     2221 ggcatgactc accagtgtca tctctgcact tcaggaattg tcaacaactg aggtggccct
     2281 tgtcagcact tcttttttta tacctcctct caaggccacg taagttacct agctctacct
     2341 gactgtggat aaaagacagt gctctaccca cctggtggac ccagggactg aagcaatggc
     2401 acagagacca tgacagactg tgctcctatc ctgggttgag tcccgatgca tattcctgct
     2461 tcatttcctt ttctcaaggg ccgcttcccc ggaccctcct gtcataggcc ccacttggtg
     2521 ctatttgtgg tgctggctca ggcgtggggc tgccaggctc gggcttggca caccaaaggc
     2581 cagctgcatg tcagtttctc tccttccaca gttatgttgg cttccatgct ttttcagatt
     2641 gtgtggtagc cataggacct gacccagttt gtgcctcctg ggatcctcat ccccaatcct
     2701 gctcttacat aaaccttttt cttgttttag taatttgtag tgatcacccc cttccctctg
     2761 ttgctgggac ccaggaggaa aggggaagat gtcctgtttc ctatcctagg tgtggacgcc
     2821 cgccttgccc tttgttagct tcctgggttc ccatgaactc agggatttgt tggaagattc
     2881 tctgcataat agatatatat gtatatatat ttaaatacac atatatattg tacagaataa
     2941 aaaatgtttt attgga
//
